Restriction map for
pF1KB6436
(Circular) (Six-base) MAPSORT of: pF1KB6436.seq Check: 7531 from: 1 to: 4046
pF1KB6436 4046 bp
With 182 enzymes: SgfI *
March 2, 2010 14:26 ..
AccI GT'mk_AC
Cuts at: 1084 1084
Size: 4046
Acc65I G'GTAC_C
Cuts at: 1062 1062
Size: 4046
AclI AA'CG_TT
Cuts at: 3886 3886
Size: 4046
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 931 1871 2303 3050 3318 931
Size: 940 432 747 268 1659
Fragments arranged by size:
1659 940 747 432 268
AflII C'TTAA_G
Cuts at: 391 391
Size: 4046
AflIII A'CryG_T
Cuts at: 1398 2502 3479 1398
Size: 1104 977 1965
Fragments arranged by size:
1965 1104 977
AgeI A'CCGG_T
Cuts at: 1038 2434 1038
Size: 1396 2650
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 486 539 486
Size: 53 3993
AlwNI CAG_nnn'CTG
Cuts at: 1351 2918 1351
Size: 1567 2479
ApaLI G'TGCA_C
Cuts at: 524 2816 524
Size: 2292 1754
ApoI r'AATT_y
Cuts at: 580 725 877 1006 1050 3399 3655 580
Size: 145 152 129 44 2349 256 971
Fragments arranged by size:
2349 971 256 152 145 129 44
AseI AT'TA_AT
Cuts at: 20 20
Size: 4046
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4046
AvaI C'yCGr_G
Cuts at: 1066 1066
Size: 4046
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1025 1058 1025
Size: 33 4013
BamHI G'GATC_C
Cuts at: 1071 1071
Size: 4046
BanI G'GyrC_C
Cuts at: 156 859 1062 1476 1724 1759 156
Size: 703 203 414 248 35 2443
Fragments arranged by size:
2443 703 414 248 203 35
BanII G_rGCy'C
Cuts at: 1060 2090 1060
Size: 1030 3016
BbeI G_GCGC'C
Cuts at: 160 1480 1728 160
Size: 1320 248 2478
Fragments arranged by size:
2478 1320 248
BbsI GAAGACnn'nnnn_
Cuts at: 951 951
Size: 4046
BbvCI CC'TCA_GC
Cuts at: 893 893
Size: 4046
BciVI GTATCCnnnnn_n'
Cuts at: 1944 2705 4046 1944
Size: 761 1341 1944
Fragments arranged by size:
1944 1341 761
BclI T'GATC_A
Cuts at: 1566 1566
Size: 4046
BglI GCCn_nnn'nGGC
Cuts at: 121 154 165 121
Size: 33 11 4002
Fragments arranged by size:
4002 33 11
BglII A'GATC_T
Cuts at: 1561 3598 1561
Size: 2037 2009
BlpI GC'TnA_GC
Cuts at: 1158 1158
Size: 4046
Bme1580I G_kGCm'C
Cuts at: 528 1671 1764 2820 528
Size: 1143 93 1056 1754
Fragments arranged by size:
1754 1143 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 934 951 1258 1339 1674 3818 934
Size: 17 307 81 335 2144 1162
Fragments arranged by size:
2144 1162 335 307 81 17
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2413 2413
Size: 4046
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 370 432 519 370
Size: 62 87 3897
Fragments arranged by size:
3897 87 62
Bpu10I CC'TnA_GC
Cuts at: 893 893
Size: 4046
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 697 812 1222 2101 2593 2891 697
Size: 115 410 879 492 298 1852
Fragments arranged by size:
1852 879 492 410 298 115
BsaI GGTCTCn'nnnn_
Cuts at: 3750 3750
Size: 4046
BsaAI yAC'GTr
Cuts at: 1399 1399
Size: 4046
BsaBI GATnn'nnATC
Cuts at: 6 1560 3597 6
Size: 1554 2037 455
Fragments arranged by size:
2037 1554 455
BsaHI Gr'CG_yC
Cuts at: 157 227 1477 1725 2427 3923 157
Size: 70 1250 248 702 1496 280
Fragments arranged by size:
1496 1250 702 280 248 70
BsaWI w'CCGG_w
Cuts at: 1038 1235 1756 2434 2708 2855 3266 1038
Size: 197 521 678 274 147 411 1818
Fragments arranged by size:
1818 678 521 411 274 197 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 362 392 651 681 362
Size: 30 259 30 3727
Fragments arranged by size:
3727 259 30 30
BseYI C'CCAG_C
Cuts at: 138 190 1471 2229 2806 3302 138
Size: 52 1281 758 577 496 882
Fragments arranged by size:
1281 882 758 577 496 52
BsiEI CG_ry'CG
Cuts at: 104 1588 1634 2842 104
Size: 1484 46 1208 1308
Fragments arranged by size:
1484 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 405 448 528 1060 1838 2028 2820 405
Size: 43 80 532 778 190 792 1631
Fragments arranged by size:
1631 792 778 532 190 80 43
Bsp1286I G_dGCh'C
Cuts at: 405 448 528 1060 1671 1764 1838 2028 2090
Size: 43 80 532 611 93 74 190 62
Cuts at: 2090 2820 405
Size: 730 1631
Fragments arranged by size:
1631 730 611 532 190 93 80 74
62 43
BspEI T'CCGG_A
Cuts at: 3266 3266
Size: 4046
BspHI T'CATG_A
Cuts at: 3222 3222
Size: 4046
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1096 1310 1612 1993 1096
Size: 214 302 381 3149
Fragments arranged by size:
3149 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2338 2392 3876 4041 50
Size: 2288 54 1484 165 55
Fragments arranged by size:
2288 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1958 1958
Size: 4046
BsrFI r'CCGG_y
Cuts at: 159 280 1038 2044 2225 2434 159
Size: 121 758 1006 181 209 1771
Fragments arranged by size:
1771 1006 758 209 181 121
BssSI C'ACGA_G
Cuts at: 2317 2675 2317
Size: 358 3688
BstAPI GCAn_nnn'nTGC
Cuts at: 1423 1423
Size: 4046
BstBI TT'CG_AA
Cuts at: 2407 2407
Size: 4046
BstXI CCAn_nnnn'nTGG
Cuts at: 132 132
Size: 4046
BstYI r'GATC_y
Cuts at: 1 1071 1538 1561 1896 2142 3154 3240 3252
Size: 1070 467 23 335 246 1012 86 12
Cuts at: 3252 3598 1
Size: 346 449
Fragments arranged by size:
1070 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 620 620
Size: 4046
BtgI C'CryG_G
Cuts at: 107 695 107
Size: 588 3458
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1996 2177 2340 1996
Size: 181 163 3702
Fragments arranged by size:
3702 181 163
BtsI GCAGTG_nn'
Cuts at: 1289 1289
Size: 4046
DraI TTT'AAA
Cuts at: 741 1045 741
Size: 304 3742
DrdI GACnn_nn'nnGTC
Cuts at: 1752 2610 1752
Size: 858 3188
EaeI y'GGCC_r
Cuts at: 216 1631 1805 2196 2223 3568 216
Size: 1415 174 391 27 1345 694
Fragments arranged by size:
1415 1345 694 391 174 27
EagI C'GGCC_G
Cuts at: 1631 1631
Size: 4046
EarI CTCTTCn'nnn_
Cuts at: 256 2069 2279 256
Size: 1813 210 2023
Fragments arranged by size:
2023 1813 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2254 2564 2710 3856 2254
Size: 310 146 1146 2444
Fragments arranged by size:
2444 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1058 1058
Size: 4046
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 370 432 519 931 1871 2303 3050 3318 370
Size: 62 87 412 940 432 747 268 1098
Fragments arranged by size:
1098 940 747 432 412 268 87 62
EcoO109I rG'GnC_Cy
Cuts at: 433 1185 433
Size: 752 3294
EcoRI G'AATT_C
Cuts at: 877 1050 877
Size: 173 3873
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2065 2065
Size: 4046
FspI TGC'GCA
Cuts at: 175 1827 175
Size: 1652 2394
HaeII r_GCGC'y
Cuts at: 160 1480 1728 2750 3729 160
Size: 1320 248 1022 979 477
Fragments arranged by size:
1320 1022 979 477 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 313 345 1888 1918 1920 1950 313
Size: 32 1543 30 2 30 2409
Fragments arranged by size:
2409 1543 32 30 30 2
HincII GTy'rAC
Cuts at: 1085 1085
Size: 4046
KasI G'GCGC_C
Cuts at: 156 1476 1724 156
Size: 1320 248 2478
Fragments arranged by size:
2478 1320 248
KpnI G_GTAC'C
Cuts at: 1066 1066
Size: 4046
MluI A'CGCG_T
Cuts at: 3479 3479
Size: 4046
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2717 2901 2717
Size: 184 3862
MscI TGG'CCA
Cuts at: 1807 1807
Size: 4046
MslI CAynn'nnrTG
Cuts at: 2035 2162 2035
Size: 127 3919
MspA1I CmG'CkG
Cuts at: 210 239 1157 1471 1831 2844 3089 210
Size: 29 918 314 360 1013 245 1167
Fragments arranged by size:
1167 1013 918 360 314 245 29
NaeI GCC'GGC
Cuts at: 161 282 2227 161
Size: 121 1945 1980
Fragments arranged by size:
1980 1945 121
NarI GG'CG_CC
Cuts at: 157 1477 1725 157
Size: 1320 248 2478
Fragments arranged by size:
2478 1320 248
NcoI C'CATG_G
Cuts at: 107 695 107
Size: 588 3458
NdeI CA'TA_TG
Cuts at: 333 333
Size: 4046
NgoMIV G'CCGG_C
Cuts at: 159 280 2225 159
Size: 121 1945 1980
Fragments arranged by size:
1980 1945 121
NspI r_CATG'y
Cuts at: 1099 1599 2506 1099
Size: 500 907 2639
Fragments arranged by size:
2639 907 500
PciI A'CATG_T
Cuts at: 2502 2502
Size: 4046
PmeI GTTT'AAAC
Cuts at: 1045 1045
Size: 4046
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3185 3217 3185
Size: 32 4014
PstI C_TGCA'G
Cuts at: 415 475 982 1093 1778 415
Size: 60 507 111 685 2683
Fragments arranged by size:
2683 685 507 111 60
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4046
PvuII CAG'CTG
Cuts at: 1471 1831 1471
Size: 360 3686
RsrII CG'GwC_CG
Cuts at: 2241 2241
Size: 4046
SacI G_AGCT'C
Cuts at: 1060 1060
Size: 4046
SalI G'TCGA_C
Cuts at: 1083 1083
Size: 4046
SapI GCTCTTCn'nnn_
Cuts at: 2069 2279 2069
Size: 210 3836
SbfI CC_TGCA'GG
Cuts at: 1093 1093
Size: 4046
ScaI AGT'ACT
Cuts at: 3563 3563
Size: 4046
SfcI C'TryA_G
Cuts at: 33 411 471 978 1089 1774 2767 2958 33
Size: 378 60 507 111 685 993 191 1121
Fragments arranged by size:
1121 993 685 507 378 191 111 60
SfoI GGC'GCC
Cuts at: 158 1478 1726 158
Size: 1320 248 2478
Fragments arranged by size:
2478 1320 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4046
SgrAI Cr'CCGG_yG
Cuts at: 159 159
Size: 4046
SmaI CCC'GGG
Cuts at: 1068 1068
Size: 4046
SmlI C'TyrA_G
Cuts at: 391 676 827 1201 2116 2608 2870 391
Size: 285 151 374 915 492 262 1567
Fragments arranged by size:
1567 915 492 374 285 262 151
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4046
SphI G_CATG'C
Cuts at: 1099 1599 1099
Size: 500 3546
SspI AAT'ATT
Cuts at: 822 872 822
Size: 50 3996
StuI AGG'CCT
Cuts at: 129 129
Size: 4046
StyI C'CwwG_G
Cuts at: 107 695 1180 1534 107
Size: 588 485 354 2619
Fragments arranged by size:
2619 588 485 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 655 1629 1762 2221 2430 655
Size: 974 133 459 209 2271
Fragments arranged by size:
2271 974 459 209 133
TatI w'GTAC_w
Cuts at: 3561 3561
Size: 4046
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 362 394 651 683 798 830 3563 3595 362
Size: 32 257 32 115 32 2733 32 813
Fragments arranged by size:
2733 813 257 115 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1843 1843
Size: 4046
XbaI T'CTAG_A
Cuts at: 1077 1077
Size: 4046
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 394 870 1541 394
Size: 476 671 2899
Fragments arranged by size:
2899 671 476
XmaI C'CCGG_G
Cuts at: 1066 1066
Size: 4046
XmnI GAAnn'nnTTC
Cuts at: 770 821 770
Size: 51 3995
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NspI PciI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AloI ApaI AscI
AvrII BcgI BfrBI BmgBI BmtI BseRI BsgI BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstZ17I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SnaBI SrfI SwaI XhoI ZraI