Restriction map for
pF1KB6437
(Circular) (Six-base) MAPSORT of: pF1KB6437.seq Check: 6201 from: 1 to: 4052
pF1KB6437 4052 bp
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AccI GT'mk_AC
Cuts at: 449 1090 449
Size: 641 3411
Acc65I G'GTAC_C
Cuts at: 1068 1068
Size: 4052
AclI AA'CG_TT
Cuts at: 3892 3892
Size: 4052
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 697 1877 2309 3056 3324 697
Size: 1180 432 747 268 1425
Fragments arranged by size:
1425 1180 747 432 268
AflIII A'CryG_T
Cuts at: 1404 2508 3485 1404
Size: 1104 977 1971
Fragments arranged by size:
1971 1104 977
AgeI A'CCGG_T
Cuts at: 2440 2440
Size: 4052
AlwNI CAG_nnn'CTG
Cuts at: 1357 2924 1357
Size: 1567 2485
ApaLI G'TGCA_C
Cuts at: 758 2822 758
Size: 2064 1988
ApoI r'AATT_y
Cuts at: 222 265 431 552 1056 3405 3661 222
Size: 43 166 121 504 2349 256 613
Fragments arranged by size:
2349 613 504 256 166 121 43
AseI AT'TA_AT
Cuts at: 20 20
Size: 4052
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4052
AvaI C'yCGr_G
Cuts at: 1072 1072
Size: 4052
AvrII C'CTAG_G
Cuts at: 978 978
Size: 4052
BamHI G'GATC_C
Cuts at: 1077 1077
Size: 4052
BanI G'GyrC_C
Cuts at: 1068 1482 1730 1765 1068
Size: 414 248 35 3355
Fragments arranged by size:
3355 414 248 35
BanII G_rGCy'C
Cuts at: 141 1066 2096 141
Size: 925 1030 2097
Fragments arranged by size:
2097 1030 925
BbeI G_GCGC'C
Cuts at: 1486 1734 1486
Size: 248 3804
BciVI GTATCCnnnnn_n'
Cuts at: 105 1950 2711 4052 105
Size: 1845 761 1341 105
Fragments arranged by size:
1845 1341 761 105
BclI T'GATC_A
Cuts at: 1572 1572
Size: 4052
BglI GCCn_nnn'nGGC
Cuts at: 182 182
Size: 4052
BglII A'GATC_T
Cuts at: 1567 3604 1567
Size: 2037 2015
BlpI GC'TnA_GC
Cuts at: 1164 1164
Size: 4052
Bme1580I G_kGCm'C
Cuts at: 762 1677 1770 2826 762
Size: 915 93 1056 1988
Fragments arranged by size:
1988 1056 915 93
BmrI ACTGGGnnnn_n'
Cuts at: 1264 1345 1680 3824 1264
Size: 81 335 2144 1492
Fragments arranged by size:
2144 1492 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 610 2419 610
Size: 1809 2243
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 391 391
Size: 4052
Bpu10I CC'TnA_GC
Cuts at: 242 581 242
Size: 339 3713
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1228 2107 2599 2897 1228
Size: 879 492 298 2383
Fragments arranged by size:
2383 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 534 3756 534
Size: 3222 830
BsaAI yAC'GTr
Cuts at: 1405 1405
Size: 4052
BsaBI GATnn'nnATC
Cuts at: 6 1566 3603 6
Size: 1560 2037 455
Fragments arranged by size:
2037 1560 455
BsaHI Gr'CG_yC
Cuts at: 1483 1731 2433 3929 1483
Size: 248 702 1496 1606
Fragments arranged by size:
1606 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1241 1762 2440 2714 2861 3272 1241
Size: 521 678 274 147 411 2021
Fragments arranged by size:
2021 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 812 842 812
Size: 30 4022
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 650 650
Size: 4052
BseYI C'CCAG_C
Cuts at: 140 163 1477 2235 2812 3308 140
Size: 23 1314 758 577 496 884
Fragments arranged by size:
1314 884 758 577 496 23
BsiEI CG_ry'CG
Cuts at: 104 1594 1640 2848 104
Size: 1490 46 1208 1308
Fragments arranged by size:
1490 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 762 1066 1844 2034 2826 762
Size: 304 778 190 792 1988
Fragments arranged by size:
1988 792 778 304 190
BsmI GAATG_Cn'
Cuts at: 406 406
Size: 4052
Bsp1286I G_dGCh'C
Cuts at: 141 762 1066 1677 1770 1844 2034 2096 2826
Size: 621 304 611 93 74 190 62 730
Cuts at: 2826 141
Size: 1367
Fragments arranged by size:
1367 730 621 611 304 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3272 3272
Size: 4052
BspHI T'CATG_A
Cuts at: 3228 3228
Size: 4052
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1102 1316 1618 1999 1102
Size: 214 302 381 3155
Fragments arranged by size:
3155 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2344 2398 3882 4047 50
Size: 2294 54 1484 165 55
Fragments arranged by size:
2294 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1964 1964
Size: 4052
BsrFI r'CCGG_y
Cuts at: 2050 2231 2440 2050
Size: 181 209 3662
Fragments arranged by size:
3662 209 181
BsrGI T'GTAC_A
Cuts at: 873 873
Size: 4052
BssSI C'ACGA_G
Cuts at: 2323 2681 2323
Size: 358 3694
BstAPI GCAn_nnn'nTGC
Cuts at: 1429 1429
Size: 4052
BstBI TT'CG_AA
Cuts at: 2413 2413
Size: 4052
BstYI r'GATC_y
Cuts at: 1 963 1077 1544 1567 1902 2148 3160 3246
Size: 962 114 467 23 335 246 1012 86
Cuts at: 3246 3258 3604 1
Size: 12 346 449
Fragments arranged by size:
1012 962 467 449 346 335 246 114
86 23 12
BtgI C'CryG_G
Cuts at: 107 254 107
Size: 147 3905
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2002 2183 2346 2002
Size: 181 163 3708
Fragments arranged by size:
3708 181 163
BtsI GCAGTG_nn'
Cuts at: 1295 1295
Size: 4052
DraI TTT'AAA
Cuts at: 393 1051 393
Size: 658 3394
DrdI GACnn_nn'nnGTC
Cuts at: 1758 2616 1758
Size: 858 3194
EaeI y'GGCC_r
Cuts at: 1637 1811 2202 2229 3574 1637
Size: 174 391 27 1345 2115
Fragments arranged by size:
2115 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1637 1637
Size: 4052
EarI CTCTTCn'nnn_
Cuts at: 149 2075 2285 149
Size: 1926 210 1916
Fragments arranged by size:
1926 1916 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2260 2570 2716 3862 2260
Size: 310 146 1146 2450
Fragments arranged by size:
2450 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1064 1064
Size: 4052
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 391 697 1877 2309 3056 3324 391
Size: 306 1180 432 747 268 1119
Fragments arranged by size:
1180 1119 747 432 306 268
EcoO109I rG'GnC_Cy
Cuts at: 1191 1191
Size: 4052
EcoRI G'AATT_C
Cuts at: 431 1056 431
Size: 625 3427
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 924 2071 924
Size: 1147 2905
FspI TGC'GCA
Cuts at: 1833 1833
Size: 4052
HaeII r_GCGC'y
Cuts at: 1486 1734 2756 3735 1486
Size: 248 1022 979 1803
Fragments arranged by size:
1803 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 364 396 582 614 1894 1924 1926 1956 364
Size: 32 186 32 1280 30 2 30 2460
Fragments arranged by size:
2460 1280 186 32 32 30 30 2
HincII GTy'rAC
Cuts at: 360 494 1091 360
Size: 134 597 3321
Fragments arranged by size:
3321 597 134
HpaI GTT'AAC
Cuts at: 360 360
Size: 4052
KasI G'GCGC_C
Cuts at: 1482 1730 1482
Size: 248 3804
KpnI G_GTAC'C
Cuts at: 1072 1072
Size: 4052
MfeI C'AATT_G
Cuts at: 932 932
Size: 4052
MluI A'CGCG_T
Cuts at: 3485 3485
Size: 4052
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 952 2723 2907 952
Size: 1771 184 2097
Fragments arranged by size:
2097 1771 184
MscI TGG'CCA
Cuts at: 1813 1813
Size: 4052
MslI CAynn'nnrTG
Cuts at: 259 641 2041 2168 259
Size: 382 1400 127 2143
Fragments arranged by size:
2143 1400 382 127
MspA1I CmG'CkG
Cuts at: 1163 1477 1837 2850 3095 1163
Size: 314 360 1013 245 2120
Fragments arranged by size:
2120 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2233 2233
Size: 4052
NarI GG'CG_CC
Cuts at: 1483 1731 1483
Size: 248 3804
NcoI C'CATG_G
Cuts at: 107 254 107
Size: 147 3905
NgoMIV G'CCGG_C
Cuts at: 2231 2231
Size: 4052
NspI r_CATG'y
Cuts at: 189 448 1105 1605 2512 189
Size: 259 657 500 907 1729
Fragments arranged by size:
1729 907 657 500 259
PciI A'CATG_T
Cuts at: 2508 2508
Size: 4052
PfoI T'CCnGG_A
Cuts at: 426 426
Size: 4052
PmeI GTTT'AAAC
Cuts at: 1051 1051
Size: 4052
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3191 3223 3191
Size: 32 4020
PstI C_TGCA'G
Cuts at: 678 1099 1784 678
Size: 421 685 2946
Fragments arranged by size:
2946 685 421
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4052
PvuII CAG'CTG
Cuts at: 1477 1837 1477
Size: 360 3692
RsrII CG'GwC_CG
Cuts at: 2247 2247
Size: 4052
SacI G_AGCT'C
Cuts at: 1066 1066
Size: 4052
SalI G'TCGA_C
Cuts at: 1089 1089
Size: 4052
SapI GCTCTTCn'nnn_
Cuts at: 2075 2285 2075
Size: 210 3842
SbfI CC_TGCA'GG
Cuts at: 1099 1099
Size: 4052
ScaI AGT'ACT
Cuts at: 3569 3569
Size: 4052
SfcI C'TryA_G
Cuts at: 33 674 774 971 984 1095 1780 2773 2964
Size: 641 100 197 13 111 685 993 191
Cuts at: 2964 33
Size: 1121
Fragments arranged by size:
1121 993 685 641 197 191 111 100
13
SfoI GGC'GCC
Cuts at: 1484 1732 1484
Size: 248 3804
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4052
SmaI CCC'GGG
Cuts at: 1074 1074
Size: 4052
SmlI C'TyrA_G
Cuts at: 1207 2122 2614 2876 1207
Size: 915 492 262 2383
Fragments arranged by size:
2383 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4052
SphI G_CATG'C
Cuts at: 189 1105 1605 189
Size: 916 500 2636
Fragments arranged by size:
2636 916 500
SspI AAT'ATT
Cuts at: 345 345
Size: 4052
StyI C'CwwG_G
Cuts at: 107 254 978 1186 1540 107
Size: 147 724 208 354 2619
Fragments arranged by size:
2619 724 354 208 147
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 495 1635 1768 2227 2436 495
Size: 1140 133 459 209 2111
Fragments arranged by size:
2111 1140 459 209 133
TatI w'GTAC_w
Cuts at: 873 3567 873
Size: 2694 1358
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 872 904 3569 3601 872
Size: 32 2665 32 1323
Fragments arranged by size:
2665 1323 32 32
Tth111I GACn'n_nGTC
Cuts at: 1849 1849
Size: 4052
XbaI T'CTAG_A
Cuts at: 1083 1083
Size: 4052
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1547 1547
Size: 4052
XmaI C'CCGG_G
Cuts at: 1072 1072
Size: 4052
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PfoI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI BaeI BbsI BbvCI BcgI BfrBI BmgBI
BmtI BsgI BsiWI BsmBI BssHII BstEII BstXI BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII NdeI NheI NotI NruI NsiI PacI PasI
PflMI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI StuI
SwaI XhoI XmnI ZraI