Restriction map for
pF1KB6438
(Circular) (Six-base) MAPSORT of: pF1KB6438.seq Check: 1264 from: 1 to: 4133
pF1KB6438 4133 bp
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 379 755 379
Size: 376 3757
AccI GT'mk_AC
Cuts at: 1171 1171
Size: 4133
Acc65I G'GTAC_C
Cuts at: 1149 1149
Size: 4133
AclI AA'CG_TT
Cuts at: 3973 3973
Size: 4133
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 631 976 978 1958 2390 3137 3405 631
Size: 345 2 980 432 747 268 1359
Fragments arranged by size:
1359 980 747 432 345 268 2
AflIII A'CryG_T
Cuts at: 293 1485 2589 3566 293
Size: 1192 1104 977 860
Fragments arranged by size:
1192 1104 977 860
AgeI A'CCGG_T
Cuts at: 2521 2521
Size: 4133
AleI CACnn'nnGTG
Cuts at: 460 460
Size: 4133
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 361 361
Size: 4133
AlwNI CAG_nnn'CTG
Cuts at: 903 1065 1438 3005 903
Size: 162 373 1567 2031
Fragments arranged by size:
2031 1567 373 162
ApaI G_GGCC'C
Cuts at: 284 419 682 929 284
Size: 135 263 247 3488
Fragments arranged by size:
3488 263 247 135
ApaLI G'TGCA_C
Cuts at: 2903 2903
Size: 4133
ApoI r'AATT_y
Cuts at: 1137 3486 3742 1137
Size: 2349 256 1528
Fragments arranged by size:
2349 1528 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4133
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4133
AvaI C'yCGr_G
Cuts at: 419 1153 419
Size: 734 3399
BamHI G'GATC_C
Cuts at: 1158 1158
Size: 4133
BanI G'GyrC_C
Cuts at: 147 217 704 1149 1563 1811 1846 147
Size: 70 487 445 414 248 35 2434
Fragments arranged by size:
2434 487 445 414 248 70 35
BanII G_rGCy'C
Cuts at: 128 284 419 682 929 1147 2177 128
Size: 156 135 263 247 218 1030 2084
Fragments arranged by size:
2084 1030 263 247 218 156 135
BbeI G_GCGC'C
Cuts at: 708 1567 1815 708
Size: 859 248 3026
Fragments arranged by size:
3026 859 248
BciVI GTATCCnnnnn_n'
Cuts at: 1036 2031 2792 4133 1036
Size: 995 761 1341 1036
Fragments arranged by size:
1341 1036 995 761
BclI T'GATC_A
Cuts at: 1653 1653
Size: 4133
BglI GCCn_nnn'nGGC
Cuts at: 279 906 279
Size: 627 3506
BglII A'GATC_T
Cuts at: 1648 3685 1648
Size: 2037 2096
BlpI GC'TnA_GC
Cuts at: 851 1245 851
Size: 394 3739
Bme1580I G_kGCm'C
Cuts at: 220 284 419 638 682 694 774 929 1758
Size: 64 135 219 44 12 80 155 829
Cuts at: 1758 1851 2907 220
Size: 93 1056 1446
Fragments arranged by size:
1446 1056 829 219 155 135 93 80
64 44 12
BmrI ACTGGGnnnn_n'
Cuts at: 923 993 1345 1426 1761 3905 923
Size: 70 352 81 335 2144 1151
Fragments arranged by size:
2144 1151 352 335 81 70
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2500 2500
Size: 4133
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 205 624 205
Size: 419 3714
Bpu10I CC'TnA_GC
Cuts at: 122 943 122
Size: 821 3312
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 485 1309 2188 2680 2978 485
Size: 824 879 492 298 1640
Fragments arranged by size:
1640 879 824 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3837 3837
Size: 4133
BsaAI yAC'GTr
Cuts at: 296 775 1024 1486 296
Size: 479 249 462 2943
Fragments arranged by size:
2943 479 462 249
BsaBI GATnn'nnATC
Cuts at: 6 1647 3684 6
Size: 1641 2037 455
Fragments arranged by size:
2037 1641 455
BsaHI Gr'CG_yC
Cuts at: 329 705 989 1564 1812 2514 4010 329
Size: 376 284 575 248 702 1496 452
Fragments arranged by size:
1496 702 575 452 376 284 248
BsaWI w'CCGG_w
Cuts at: 1322 1843 2521 2795 2942 3353 1322
Size: 521 678 274 147 411 2102
Fragments arranged by size:
2102 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1054 1057 1060 1054
Size: 3 3 4127
Fragments arranged by size:
4127 3 3
BseYI C'CCAG_C
Cuts at: 154 163 431 1558 2316 2893 3389 154
Size: 9 268 1127 758 577 496 898
Fragments arranged by size:
1127 898 758 577 496 268 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1093 1093
Size: 4133
BsiEI CG_ry'CG
Cuts at: 104 1675 1721 2929 104
Size: 1571 46 1208 1308
Fragments arranged by size:
1571 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 128 1052 1147 1925 2115 2907 128
Size: 924 95 778 190 792 1354
Fragments arranged by size:
1354 924 792 778 190 95
Bsp1286I G_dGCh'C
Cuts at: 128 220 284 419 638 682 694 774 929
Size: 92 64 135 219 44 12 80 155
Cuts at: 929 1052 1147 1758 1851 1925 2115 2177 2907
Size: 123 95 611 93 74 190 62 730
Cuts at: 2907 128
Size: 1354
Fragments arranged by size:
1354 730 611 219 190 155 135 123
95 93 92 80 74 64 62 44
12
BspEI T'CCGG_A
Cuts at: 3353 3353
Size: 4133
BspHI T'CATG_A
Cuts at: 560 3309 560
Size: 2749 1384
BspMI ACCTGCnnnn'nnnn_
Cuts at: 379 731 755 1183 1397 1699 2080 379
Size: 352 24 428 214 302 381 2432
Fragments arranged by size:
2432 428 381 352 302 214 24
BsrBI CCG'CTC
Cuts at: 50 450 2425 2479 3963 4128 50
Size: 400 1975 54 1484 165 55
Fragments arranged by size:
1975 1484 400 165 55 54
BsrDI GCAATG_nn'
Cuts at: 357 2045 357
Size: 1688 2445
BsrFI r'CCGG_y
Cuts at: 2131 2312 2521 2131
Size: 181 209 3743
Fragments arranged by size:
3743 209 181
BssHII G'CGCG_C
Cuts at: 159 353 389 846 159
Size: 194 36 457 3446
Fragments arranged by size:
3446 457 194 36
BssSI C'ACGA_G
Cuts at: 2404 2762 2404
Size: 358 3775
BstAPI GCAn_nnn'nTGC
Cuts at: 224 903 1510 224
Size: 679 607 2847
Fragments arranged by size:
2847 679 607
BstBI TT'CG_AA
Cuts at: 2494 2494
Size: 4133
BstYI r'GATC_y
Cuts at: 1 1061 1094 1158 1625 1648 1983 2229 3241
Size: 1060 33 64 467 23 335 246 1012
Cuts at: 3241 3327 3339 3685 1
Size: 86 12 346 449
Fragments arranged by size:
1060 1012 467 449 346 335 246 86
64 33 23 12
BtgI C'CryG_G
Cuts at: 255 275 440 255
Size: 20 165 3948
Fragments arranged by size:
3948 165 20
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 192 2083 2264 2427 192
Size: 1891 181 163 1898
Fragments arranged by size:
1898 1891 181 163
BtsI GCAGTG_nn'
Cuts at: 355 1376 355
Size: 1021 3112
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 641 676 641
Size: 35 4098
DraI TTT'AAA
Cuts at: 1132 1132
Size: 4133
DraIII CAC_nnn'GTG
Cuts at: 224 299 224
Size: 75 4058
DrdI GACnn_nn'nnGTC
Cuts at: 1839 2697 1839
Size: 858 3275
EaeI y'GGCC_r
Cuts at: 252 258 861 1718 1892 2283 2310 3655 252
Size: 6 603 857 174 391 27 1345 730
Fragments arranged by size:
1345 857 730 603 391 174 27 6
EagI C'GGCC_G
Cuts at: 1718 1718
Size: 4133
EarI CTCTTCn'nnn_
Cuts at: 562 2156 2366 562
Size: 1594 210 2329
Fragments arranged by size:
2329 1594 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1045 2341 2651 2797 3943 1045
Size: 1296 310 146 1146 1235
Fragments arranged by size:
1296 1235 1146 310 146
EcoICRI GAG'CTC
Cuts at: 126 1145 126
Size: 1019 3114
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 205 624 631 976 978 1958 2390 3137 3405
Size: 419 7 345 2 980 432 747 268
Cuts at: 3405 205
Size: 933
Fragments arranged by size:
980 933 747 432 419 345 268 7
2
EcoNI CCTnn'n_nnAGG
Cuts at: 727 727
Size: 4133
EcoO109I rG'GnC_Cy
Cuts at: 280 416 423 678 789 925 939 1104 1272
Size: 136 7 255 111 136 14 165 168
Cuts at: 1272 280
Size: 3141
Fragments arranged by size:
3141 255 168 165 136 136 111 14
7
EcoRI G'AATT_C
Cuts at: 1137 1137
Size: 4133
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2152 2152
Size: 4133
FspI TGC'GCA
Cuts at: 205 337 1914 205
Size: 132 1577 2424
Fragments arranged by size:
2424 1577 132
HaeII r_GCGC'y
Cuts at: 708 1567 1815 2837 3816 708
Size: 859 248 1022 979 1025
Fragments arranged by size:
1025 1022 979 859 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1975 2005 2007 2037 1975
Size: 30 2 30 4071
Fragments arranged by size:
4071 30 30 2
HincII GTy'rAC
Cuts at: 1172 1172
Size: 4133
KasI G'GCGC_C
Cuts at: 704 1563 1811 704
Size: 859 248 3026
Fragments arranged by size:
3026 859 248
KpnI G_GTAC'C
Cuts at: 1153 1153
Size: 4133
MluI A'CGCG_T
Cuts at: 3566 3566
Size: 4133
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 866 2804 2988 866
Size: 1938 184 2011
Fragments arranged by size:
2011 1938 184
MscI TGG'CCA
Cuts at: 260 1894 260
Size: 1634 2499
MslI CAynn'nnrTG
Cuts at: 460 749 2122 2249 460
Size: 289 1373 127 2344
Fragments arranged by size:
2344 1373 289 127
MspA1I CmG'CkG
Cuts at: 154 243 277 383 442 609 1035 1244 1558
Size: 89 34 106 59 167 426 209 314
Cuts at: 1558 1918 2931 3176 154
Size: 360 1013 245 1111
Fragments arranged by size:
1111 1013 426 360 314 245 209 167
106 89 59 34
NaeI GCC'GGC
Cuts at: 2314 2314
Size: 4133
NarI GG'CG_CC
Cuts at: 705 1564 1812 705
Size: 859 248 3026
Fragments arranged by size:
3026 859 248
NgoMIV G'CCGG_C
Cuts at: 2312 2312
Size: 4133
NspI r_CATG'y
Cuts at: 754 1002 1186 1686 2593 754
Size: 248 184 500 907 2294
Fragments arranged by size:
2294 907 500 248 184
PciI A'CATG_T
Cuts at: 2589 2589
Size: 4133
PmeI GTTT'AAAC
Cuts at: 1132 1132
Size: 4133
PmlI CAC'GTG
Cuts at: 296 775 296
Size: 479 3654
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3272 3304 3272
Size: 32 4101
PpuMI rG'GwC_Cy
Cuts at: 423 423
Size: 4133
PspOMI G'GGCC_C
Cuts at: 280 415 678 925 280
Size: 135 263 247 3488
Fragments arranged by size:
3488 263 247 135
PstI C_TGCA'G
Cuts at: 350 1034 1070 1180 1865 350
Size: 684 36 110 685 2618
Fragments arranged by size:
2618 685 684 110 36
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4133
PvuII CAG'CTG
Cuts at: 243 609 1558 1918 243
Size: 366 949 360 2458
Fragments arranged by size:
2458 949 366 360
RsrII CG'GwC_CG
Cuts at: 2328 2328
Size: 4133
SacI G_AGCT'C
Cuts at: 128 1147 128
Size: 1019 3114
SacII CC_GC'GG
Cuts at: 278 443 278
Size: 165 3968
SalI G'TCGA_C
Cuts at: 1170 1170
Size: 4133
SapI GCTCTTCn'nnn_
Cuts at: 2156 2366 2156
Size: 210 3923
SbfI CC_TGCA'GG
Cuts at: 1180 1180
Size: 4133
ScaI AGT'ACT
Cuts at: 1075 3650 1075
Size: 2575 1558
SfcI C'TryA_G
Cuts at: 33 346 1030 1066 1176 1861 2854 3045 33
Size: 313 684 36 110 685 993 191 1121
Fragments arranged by size:
1121 993 685 684 313 191 110 36
SfoI GGC'GCC
Cuts at: 706 1565 1813 706
Size: 859 248 3026
Fragments arranged by size:
3026 859 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4133
SmaI CCC'GGG
Cuts at: 421 1155 421
Size: 734 3399
SmlI C'TyrA_G
Cuts at: 500 1288 2203 2695 2957 500
Size: 788 915 492 262 1676
Fragments arranged by size:
1676 915 788 492 262
SnaBI TAC'GTA
Cuts at: 1024 1024
Size: 4133
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4133
SphI G_CATG'C
Cuts at: 1002 1186 1686 1002
Size: 184 500 3449
Fragments arranged by size:
3449 500 184
StuI AGG'CCT
Cuts at: 344 344
Size: 4133
StyI C'CwwG_G
Cuts at: 728 1122 1267 1621 728
Size: 394 145 354 3240
Fragments arranged by size:
3240 394 354 145
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1716 1849 2308 2517 1716
Size: 133 459 209 3332
Fragments arranged by size:
3332 459 209 133
TatI w'GTAC_w
Cuts at: 530 869 1073 3648 530
Size: 339 204 2575 1015
Fragments arranged by size:
2575 1015 339 204
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3650 3682 3650
Size: 32 4101
Tth111I GACn'n_nGTC
Cuts at: 1930 1930
Size: 4133
XbaI T'CTAG_A
Cuts at: 1164 1164
Size: 4133
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 240 1628 240
Size: 1388 2745
XmaI C'CCGG_G
Cuts at: 419 1153 419
Size: 734 3399
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PciI PmeI PmlI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AloI AscI AvrII BaeI
BbsI BbvCI BcgI BfrBI BmgBI BmtI BsaXI BsiWI
BsmI BsmBI BsrGI BstEII BstXI BstZ17I Bsu36I ClaI
EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PshAI PsiI PspXI PsrI SanDI SexAI SfiI SgrAI
SrfI SspI SwaI XhoI XmnI ZraI