Restriction map for
pF1KB6439
(Circular) (Six-base) MAPSORT of: pF1KB6439.seq Check: 6941 from: 1 to: 4055
pF1KB6439 4055 bp
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AccI GT'mk_AC
Cuts at: 804 1093 804
Size: 289 3766
Acc65I G'GTAC_C
Cuts at: 1071 1071
Size: 4055
AclI AA'CG_TT
Cuts at: 3895 3895
Size: 4055
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1880 2312 3059 3327 1880
Size: 432 747 268 2608
Fragments arranged by size:
2608 747 432 268
AflIII A'CryG_T
Cuts at: 859 1407 2511 3488 859
Size: 548 1104 977 1426
Fragments arranged by size:
1426 1104 977 548
AgeI A'CCGG_T
Cuts at: 2443 2443
Size: 4055
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 595 595
Size: 4055
AlwNI CAG_nnn'CTG
Cuts at: 1360 2927 1360
Size: 1567 2488
ApaLI G'TGCA_C
Cuts at: 633 2825 633
Size: 2192 1863
ApoI r'AATT_y
Cuts at: 265 618 1059 3408 3664 265
Size: 353 441 2349 256 656
Fragments arranged by size:
2349 656 441 353 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4055
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4055
AvaI C'yCGr_G
Cuts at: 1075 1075
Size: 4055
BamHI G'GATC_C
Cuts at: 979 1080 979
Size: 101 3954
BanI G'GyrC_C
Cuts at: 1071 1485 1733 1768 1071
Size: 414 248 35 3358
Fragments arranged by size:
3358 414 248 35
BanII G_rGCy'C
Cuts at: 500 1069 2099 500
Size: 569 1030 2456
Fragments arranged by size:
2456 1030 569
BbeI G_GCGC'C
Cuts at: 1489 1737 1489
Size: 248 3807
BbsI GAAGACnn'nnnn_
Cuts at: 436 436
Size: 4055
BciVI GTATCCnnnnn_n'
Cuts at: 1953 2714 4055 1953
Size: 761 1341 1953
Fragments arranged by size:
1953 1341 761
BclI T'GATC_A
Cuts at: 1575 1575
Size: 4055
BglII A'GATC_T
Cuts at: 1570 3607 1570
Size: 2037 2018
BlpI GC'TnA_GC
Cuts at: 1167 1167
Size: 4055
Bme1580I G_kGCm'C
Cuts at: 605 637 1680 1773 2829 605
Size: 32 1043 93 1056 1831
Fragments arranged by size:
1831 1056 1043 93 32
BmrI ACTGGGnnnn_n'
Cuts at: 684 743 1023 1267 1348 1683 3827 684
Size: 59 280 244 81 335 2144 912
Fragments arranged by size:
2144 912 335 280 244 81 59
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2422 2422
Size: 4055
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 769 1231 2110 2602 2900 769
Size: 462 879 492 298 1924
Fragments arranged by size:
1924 879 492 462 298
BsaI GGTCTCn'nnnn_
Cuts at: 475 3759 475
Size: 3284 771
BsaAI yAC'GTr
Cuts at: 135 1408 135
Size: 1273 2782
BsaBI GATnn'nnATC
Cuts at: 6 1569 3606 6
Size: 1563 2037 455
Fragments arranged by size:
2037 1563 455
BsaHI Gr'CG_yC
Cuts at: 1486 1734 2436 3932 1486
Size: 248 702 1496 1609
Fragments arranged by size:
1609 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1244 1765 2443 2717 2864 3275 1244
Size: 521 678 274 147 411 2024
Fragments arranged by size:
2024 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1480 2238 2815 3311 1480
Size: 758 577 496 2224
Fragments arranged by size:
2224 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1597 1643 2851 104
Size: 1493 46 1208 1308
Fragments arranged by size:
1493 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 637 1069 1847 2037 2829 637
Size: 432 778 190 792 1863
Fragments arranged by size:
1863 792 778 432 190
Bsp1286I G_dGCh'C
Cuts at: 500 605 637 1069 1680 1773 1847 2037 2099
Size: 105 32 432 611 93 74 190 62
Cuts at: 2099 2829 500
Size: 730 1726
Fragments arranged by size:
1726 730 611 432 190 105 93 74
62 32
BspEI T'CCGG_A
Cuts at: 3275 3275
Size: 4055
BspHI T'CATG_A
Cuts at: 639 3231 639
Size: 2592 1463
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1105 1319 1621 2002 1105
Size: 214 302 381 3158
Fragments arranged by size:
3158 381 302 214
BsrBI CCG'CTC
Cuts at: 50 518 2347 2401 3885 4050 50
Size: 468 1829 54 1484 165 55
Fragments arranged by size:
1829 1484 468 165 55 54
BsrDI GCAATG_nn'
Cuts at: 500 1967 500
Size: 1467 2588
BsrFI r'CCGG_y
Cuts at: 561 2053 2234 2443 561
Size: 1492 181 209 2173
Fragments arranged by size:
2173 1492 209 181
BsrGI T'GTAC_A
Cuts at: 533 533
Size: 4055
BssSI C'ACGA_G
Cuts at: 2326 2684 2326
Size: 358 3697
BstAPI GCAn_nnn'nTGC
Cuts at: 283 1432 283
Size: 1149 2906
BstBI TT'CG_AA
Cuts at: 2416 2416
Size: 4055
BstEII G'GTnAC_C
Cuts at: 909 909
Size: 4055
BstYI r'GATC_y
Cuts at: 1 979 1080 1547 1570 1905 2151 3163 3249
Size: 978 101 467 23 335 246 1012 86
Cuts at: 3249 3261 3607 1
Size: 12 346 449
Fragments arranged by size:
1012 978 467 449 346 335 246 101
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 527 527
Size: 4055
BtgI C'CryG_G
Cuts at: 107 812 107
Size: 705 3350
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2005 2186 2349 2005
Size: 181 163 3711
Fragments arranged by size:
3711 181 163
BtsI GCAGTG_nn'
Cuts at: 1298 1298
Size: 4055
DraI TTT'AAA
Cuts at: 1054 1054
Size: 4055
DrdI GACnn_nn'nnGTC
Cuts at: 1761 2619 1761
Size: 858 3197
EaeI y'GGCC_r
Cuts at: 110 350 567 815 999 1640 1814 2205 2232
Size: 240 217 248 184 641 174 391 27
Cuts at: 2232 3577 110
Size: 1345 588
Fragments arranged by size:
1345 641 588 391 248 240 217 184
174 27
EagI C'GGCC_G
Cuts at: 1640 1640
Size: 4055
EarI CTCTTCn'nnn_
Cuts at: 384 434 2078 2288 384
Size: 50 1644 210 2151
Fragments arranged by size:
2151 1644 210 50
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2263 2573 2719 3865 2263
Size: 310 146 1146 2453
Fragments arranged by size:
2453 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1067 1067
Size: 4055
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1880 2312 3059 3327 1880
Size: 432 747 268 2608
Fragments arranged by size:
2608 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1194 1194
Size: 4055
EcoRI G'AATT_C
Cuts at: 1059 1059
Size: 4055
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 144 2074 144
Size: 1930 2125
FspI TGC'GCA
Cuts at: 1836 1836
Size: 4055
HaeII r_GCGC'y
Cuts at: 1489 1737 2759 3738 1489
Size: 248 1022 979 1806
Fragments arranged by size:
1806 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1897 1927 1929 1959 1897
Size: 30 2 30 3993
Fragments arranged by size:
3993 30 30 2
HincII GTy'rAC
Cuts at: 1094 1094
Size: 4055
KasI G'GCGC_C
Cuts at: 1485 1733 1485
Size: 248 3807
KpnI G_GTAC'C
Cuts at: 1075 1075
Size: 4055
MluI A'CGCG_T
Cuts at: 3488 3488
Size: 4055
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2726 2910 2726
Size: 184 3871
MscI TGG'CCA
Cuts at: 112 352 817 1001 1816 112
Size: 240 465 184 815 2351
Fragments arranged by size:
2351 815 465 240 184
MslI CAynn'nnrTG
Cuts at: 577 2044 2171 577
Size: 1467 127 2461
Fragments arranged by size:
2461 1467 127
MspA1I CmG'CkG
Cuts at: 626 1166 1480 1840 2853 3098 626
Size: 540 314 360 1013 245 1583
Fragments arranged by size:
1583 1013 540 360 314 245
NaeI GCC'GGC
Cuts at: 563 2236 563
Size: 1673 2382
NarI GG'CG_CC
Cuts at: 1486 1734 1486
Size: 248 3807
NcoI C'CATG_G
Cuts at: 107 812 107
Size: 705 3350
NgoMIV G'CCGG_C
Cuts at: 561 2234 561
Size: 1673 2382
NspI r_CATG'y
Cuts at: 863 895 1108 1608 2515 863
Size: 32 213 500 907 2403
Fragments arranged by size:
2403 907 500 213 32
PciI A'CATG_T
Cuts at: 859 2511 859
Size: 1652 2403
PmeI GTTT'AAAC
Cuts at: 1054 1054
Size: 4055
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3194 3226 3194
Size: 32 4023
PstI C_TGCA'G
Cuts at: 291 1102 1787 291
Size: 811 685 2559
Fragments arranged by size:
2559 811 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4055
PvuII CAG'CTG
Cuts at: 1480 1840 1480
Size: 360 3695
RsrII CG'GwC_CG
Cuts at: 2250 2250
Size: 4055
SacI G_AGCT'C
Cuts at: 1069 1069
Size: 4055
SalI G'TCGA_C
Cuts at: 1092 1092
Size: 4055
SapI GCTCTTCn'nnn_
Cuts at: 384 2078 2288 384
Size: 1694 210 2151
Fragments arranged by size:
2151 1694 210
SbfI CC_TGCA'GG
Cuts at: 1102 1102
Size: 4055
ScaI AGT'ACT
Cuts at: 3572 3572
Size: 4055
SexAI A'CCwGG_T
Cuts at: 798 798
Size: 4055
SfcI C'TryA_G
Cuts at: 33 287 1098 1783 2776 2967 33
Size: 254 811 685 993 191 1121
Fragments arranged by size:
1121 993 811 685 254 191
SfoI GGC'GCC
Cuts at: 1487 1735 1487
Size: 248 3807
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4055
SmaI CCC'GGG
Cuts at: 1077 1077
Size: 4055
SmlI C'TyrA_G
Cuts at: 784 1210 2125 2617 2879 784
Size: 426 915 492 262 1960
Fragments arranged by size:
1960 915 492 426 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4055
SphI G_CATG'C
Cuts at: 1108 1608 1108
Size: 500 3555
StuI AGG'CCT
Cuts at: 920 920
Size: 4055
StyI C'CwwG_G
Cuts at: 107 812 959 1189 1543 107
Size: 705 147 230 354 2619
Fragments arranged by size:
2619 705 354 230 147
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 127 1638 1771 2230 2439 127
Size: 1511 133 459 209 1743
Fragments arranged by size:
1743 1511 459 209 133
TatI w'GTAC_w
Cuts at: 533 3570 533
Size: 3037 1018
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3572 3604 3572
Size: 32 4023
Tth111I GACn'n_nGTC
Cuts at: 800 1852 800
Size: 1052 3003
XbaI T'CTAG_A
Cuts at: 1086 1086
Size: 4055
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 730 1550 730
Size: 820 3235
XmaI C'CCGG_G
Cuts at: 1075 1075
Size: 4055
XmnI GAAnn'nnTTC
Cuts at: 760 760
Size: 4055
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI BpmI Bpu10I BsaXI BseRI BsgI BsiWI BsmI
BsmBI BssHII BstXI BstZ17I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI ZraI