Restriction map for
pF1KB6442
(Circular) (Six-base) MAPSORT of: pF1KB6442.seq Check: 7216 from: 1 to: 4151
pF1KB6442 4151 bp
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 542 542
Size: 4151
AatII G_ACGT'C
Cuts at: 973 973
Size: 4151
AccI GT'mk_AC
Cuts at: 1189 1189
Size: 4151
Acc65I G'GTAC_C
Cuts at: 716 1167 716
Size: 451 3700
AclI AA'CG_TT
Cuts at: 3991 3991
Size: 4151
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 195 250 421 1976 2408 3155 3423 195
Size: 55 171 1555 432 747 268 923
Fragments arranged by size:
1555 923 747 432 268 171 55
AflIII A'CryG_T
Cuts at: 1503 2607 3584 1503
Size: 1104 977 2070
Fragments arranged by size:
2070 1104 977
AgeI A'CCGG_T
Cuts at: 2539 2539
Size: 4151
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 169 201 169
Size: 32 4119
AlwNI CAG_nnn'CTG
Cuts at: 746 1141 1456 3023 746
Size: 395 315 1567 1874
Fragments arranged by size:
1874 1567 395 315
ApaLI G'TGCA_C
Cuts at: 2921 2921
Size: 4151
ApoI r'AATT_y
Cuts at: 1155 3504 3760 1155
Size: 2349 256 1546
Fragments arranged by size:
2349 1546 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4151
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4151
AvaI C'yCGr_G
Cuts at: 994 1171 994
Size: 177 3974
AvrII C'CTAG_G
Cuts at: 216 216
Size: 4151
BamHI G'GATC_C
Cuts at: 1176 1176
Size: 4151
BanI G'GyrC_C
Cuts at: 716 1167 1581 1829 1864 716
Size: 451 414 248 35 3003
Fragments arranged by size:
3003 451 414 248 35
BanII G_rGCy'C
Cuts at: 167 481 509 836 1165 2195 167
Size: 314 28 327 329 1030 2123
Fragments arranged by size:
2123 1030 329 327 314 28
BbeI G_GCGC'C
Cuts at: 1585 1833 1585
Size: 248 3903
BbsI GAAGACnn'nnnn_
Cuts at: 318 574 318
Size: 256 3895
BciVI GTATCCnnnnn_n'
Cuts at: 2049 2810 4151 2049
Size: 761 1341 2049
Fragments arranged by size:
2049 1341 761
BclI T'GATC_A
Cuts at: 1671 1671
Size: 4151
BglI GCCn_nnn'nGGC
Cuts at: 1138 1138
Size: 4151
BglII A'GATC_T
Cuts at: 347 1666 3703 347
Size: 1319 2037 795
Fragments arranged by size:
2037 1319 795
BlpI GC'TnA_GC
Cuts at: 871 1263 871
Size: 392 3759
Bme1580I G_kGCm'C
Cuts at: 290 1776 1869 2925 290
Size: 1486 93 1056 1516
Fragments arranged by size:
1516 1486 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1363 1444 1779 3923 1363
Size: 81 335 2144 1591
Fragments arranged by size:
2144 1591 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2518 2518
Size: 4151
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 505 1051 1087 505
Size: 546 36 3569
Fragments arranged by size:
3569 546 36
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 493 1327 2206 2698 2996 493
Size: 834 879 492 298 1648
Fragments arranged by size:
1648 879 834 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1042 3855 1042
Size: 2813 1338
BsaAI yAC'GTr
Cuts at: 1504 1504
Size: 4151
BsaBI GATnn'nnATC
Cuts at: 6 1665 3702 6
Size: 1659 2037 455
Fragments arranged by size:
2037 1659 455
BsaHI Gr'CG_yC
Cuts at: 359 731 970 1582 1830 2532 4028 359
Size: 372 239 612 248 702 1496 482
Fragments arranged by size:
1496 702 612 482 372 248 239
BsaWI w'CCGG_w
Cuts at: 1340 1861 2539 2813 2960 3371 1340
Size: 521 678 274 147 411 2120
Fragments arranged by size:
2120 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 169 199 655 685 169
Size: 30 456 30 3635
Fragments arranged by size:
3635 456 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 264 844 907 264
Size: 580 63 3508
Fragments arranged by size:
3508 580 63
BseYI C'CCAG_C
Cuts at: 340 369 1083 1576 2334 2911 3407 340
Size: 29 714 493 758 577 496 1084
Fragments arranged by size:
1084 758 714 577 496 493 29
BsiEI CG_ry'CG
Cuts at: 104 1693 1739 2947 104
Size: 1589 46 1208 1308
Fragments arranged by size:
1589 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 836 1165 1943 2133 2925 836
Size: 329 778 190 792 2062
Fragments arranged by size:
2062 792 778 329 190
Bsp1286I G_dGCh'C
Cuts at: 167 290 481 509 836 1165 1776 1869 1943
Size: 123 191 28 327 329 611 93 74
Cuts at: 1943 2133 2195 2925 167
Size: 190 62 730 1393
Fragments arranged by size:
1393 730 611 329 327 191 190 123
93 74 62 28
BspEI T'CCGG_A
Cuts at: 3371 3371
Size: 4151
BspHI T'CATG_A
Cuts at: 173 560 890 3327 173
Size: 387 330 2437 997
Fragments arranged by size:
2437 997 387 330
BspMI ACCTGCnnnn'nnnn_
Cuts at: 542 1201 1415 1717 2098 542
Size: 659 214 302 381 2595
Fragments arranged by size:
2595 659 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2443 2497 3981 4146 50
Size: 2393 54 1484 165 55
Fragments arranged by size:
2393 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2063 2063
Size: 4151
BsrFI r'CCGG_y
Cuts at: 2149 2330 2539 2149
Size: 181 209 3761
Fragments arranged by size:
3761 209 181
BssSI C'ACGA_G
Cuts at: 2422 2780 2422
Size: 358 3793
BstAPI GCAn_nnn'nTGC
Cuts at: 1528 1528
Size: 4151
BstBI TT'CG_AA
Cuts at: 2512 2512
Size: 4151
BstEII G'GTnAC_C
Cuts at: 936 936
Size: 4151
BstYI r'GATC_y
Cuts at: 1 347 1176 1643 1666 2001 2247 3259 3345
Size: 346 829 467 23 335 246 1012 86
Cuts at: 3345 3357 3703 1
Size: 12 346 449
Fragments arranged by size:
1012 829 467 449 346 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 763 899 107
Size: 656 136 3359
Fragments arranged by size:
3359 656 136
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 269 2101 2282 2445 269
Size: 1832 181 163 1975
Fragments arranged by size:
1975 1832 181 163
BtsI GCAGTG_nn'
Cuts at: 1394 1394
Size: 4151
DraI TTT'AAA
Cuts at: 1150 1150
Size: 4151
DrdI GACnn_nn'nnGTC
Cuts at: 1857 2715 1857
Size: 858 3293
EaeI y'GGCC_r
Cuts at: 1736 1910 2301 2328 3673 1736
Size: 174 391 27 1345 2214
Fragments arranged by size:
2214 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1736 1736
Size: 4151
EarI CTCTTCn'nnn_
Cuts at: 433 448 934 1030 2174 2384 433
Size: 15 486 96 1144 210 2200
Fragments arranged by size:
2200 1144 486 210 96 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2359 2669 2815 3961 2359
Size: 310 146 1146 2549
Fragments arranged by size:
2549 1146 310 146
EcoICRI GAG'CTC
Cuts at: 834 1163 834
Size: 329 3822
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 195 250 421 505 1051 1087 1976 2408 3155
Size: 55 171 84 546 36 889 432 747
Cuts at: 3155 3423 195
Size: 268 923
Fragments arranged by size:
923 889 747 546 432 268 171 84
55 36
EcoO109I rG'GnC_Cy
Cuts at: 1131 1290 1131
Size: 159 3992
EcoRI G'AATT_C
Cuts at: 1155 1155
Size: 4151
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2170 2170
Size: 4151
FspI TGC'GCA
Cuts at: 1932 1932
Size: 4151
HaeII r_GCGC'y
Cuts at: 115 1585 1833 2855 3834 115
Size: 1470 248 1022 979 432
Fragments arranged by size:
1470 1022 979 432 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 636 655 668 687 970 1002 1993 2023 2025
Size: 19 13 19 283 32 991 30 2
Cuts at: 2025 2055 636
Size: 30 2732
Fragments arranged by size:
2732 991 283 32 30 30 19 19
13 2
HincII GTy'rAC
Cuts at: 1190 1190
Size: 4151
KasI G'GCGC_C
Cuts at: 1581 1829 1581
Size: 248 3903
KpnI G_GTAC'C
Cuts at: 720 1171 720
Size: 451 3700
MfeI C'AATT_G
Cuts at: 947 947
Size: 4151
MluI A'CGCG_T
Cuts at: 3584 3584
Size: 4151
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1013 2822 3006 1013
Size: 1809 184 2158
Fragments arranged by size:
2158 1809 184
MscI TGG'CCA
Cuts at: 1912 1912
Size: 4151
MslI CAynn'nnrTG
Cuts at: 577 2140 2267 577
Size: 1563 127 2461
Fragments arranged by size:
2461 1563 127
MspA1I CmG'CkG
Cuts at: 743 1262 1576 1936 2949 3194 743
Size: 519 314 360 1013 245 1700
Fragments arranged by size:
1700 1013 519 360 314 245
NaeI GCC'GGC
Cuts at: 2332 2332
Size: 4151
NarI GG'CG_CC
Cuts at: 1582 1830 1582
Size: 248 3903
NcoI C'CATG_G
Cuts at: 107 899 107
Size: 792 3359
NgoMIV G'CCGG_C
Cuts at: 2330 2330
Size: 4151
NspI r_CATG'y
Cuts at: 582 1204 1704 2611 582
Size: 622 500 907 2122
Fragments arranged by size:
2122 907 622 500
PasI CC'CwG_GG
Cuts at: 495 495
Size: 4151
PciI A'CATG_T
Cuts at: 2607 2607
Size: 4151
PflMI CCAn_nnn'nTGG
Cuts at: 1141 1141
Size: 4151
PmeI GTTT'AAAC
Cuts at: 1150 1150
Size: 4151
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 169 201 3290 3322 169
Size: 32 3089 32 998
Fragments arranged by size:
3089 998 32 32
PstI C_TGCA'G
Cuts at: 587 859 1198 1883 587
Size: 272 339 685 2855
Fragments arranged by size:
2855 685 339 272
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4151
PvuII CAG'CTG
Cuts at: 743 1576 1936 743
Size: 833 360 2958
Fragments arranged by size:
2958 833 360
RsrII CG'GwC_CG
Cuts at: 2346 2346
Size: 4151
SacI G_AGCT'C
Cuts at: 836 1165 836
Size: 329 3822
SalI G'TCGA_C
Cuts at: 1188 1188
Size: 4151
SapI GCTCTTCn'nnn_
Cuts at: 934 1030 2174 2384 934
Size: 96 1144 210 2701
Fragments arranged by size:
2701 1144 210 96
SbfI CC_TGCA'GG
Cuts at: 1198 1198
Size: 4151
ScaI AGT'ACT
Cuts at: 526 3668 526
Size: 3142 1009
SexAI A'CCwGG_T
Cuts at: 719 1097 719
Size: 378 3773
SfcI C'TryA_G
Cuts at: 33 583 807 855 879 964 1194 1879 2872
Size: 550 224 48 24 85 230 685 993
Cuts at: 2872 3063 33
Size: 191 1121
Fragments arranged by size:
1121 993 685 550 230 224 191 85
48 24
SfoI GGC'GCC
Cuts at: 1583 1831 1583
Size: 248 3903
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4151
SmaI CCC'GGG
Cuts at: 1173 1173
Size: 4151
SmlI C'TyrA_G
Cuts at: 508 1306 2221 2713 2975 508
Size: 798 915 492 262 1684
Fragments arranged by size:
1684 915 798 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4151
SphI G_CATG'C
Cuts at: 1204 1704 1204
Size: 500 3651
StuI AGG'CCT
Cuts at: 1060 1060
Size: 4151
StyI C'CwwG_G
Cuts at: 107 159 216 501 899 1285 1639 107
Size: 52 57 285 398 386 354 2619
Fragments arranged by size:
2619 398 386 354 285 57 52
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 834 892 1734 1867 2326 2535 834
Size: 58 842 133 459 209 2450
Fragments arranged by size:
2450 842 459 209 133 58
TatI w'GTAC_w
Cuts at: 524 3666 524
Size: 3142 1009
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3668 3700 3668
Size: 32 4119
Tth111I GACn'n_nGTC
Cuts at: 730 1948 730
Size: 1218 2933
XbaI T'CTAG_A
Cuts at: 1182 1182
Size: 4151
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1646 1646
Size: 4151
XmaI C'CCGG_G
Cuts at: 1171 1171
Size: 4151
XmnI GAAnn'nnTTC
Cuts at: 181 181
Size: 4151
ZraI GAC'GTC
Cuts at: 971 971
Size: 4151
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbsI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PflMI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AleI AlfI ApaI AscI BaeI
BbvCI BcgI BfrBI BmgBI BmtI Bpu10I BsgI BsiWI
BsmI BsmBI BsrGI BssHII BstXI BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
NdeI NheI NotI NruI NsiI PacI PfoI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI