Restriction map for
pF1KB6445
(Circular) (Six-base) MAPSORT of: pF1KB6445.seq Check: 3146 from: 1 to: 4058
pF1KB6445 4058 bp
With 182 enzymes: SgfI *
March 2, 2010 14:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 390 672 390
Size: 282 3776
AccI GT'mk_AC
Cuts at: 410 758 1010 1096 410
Size: 348 252 86 3372
Fragments arranged by size:
3372 348 252 86
Acc65I G'GTAC_C
Cuts at: 1074 1074
Size: 4058
AclI AA'CG_TT
Cuts at: 3898 3898
Size: 4058
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 238 1883 2315 3062 3330 238
Size: 1645 432 747 268 966
Fragments arranged by size:
1645 966 747 432 268
AflIII A'CryG_T
Cuts at: 659 1410 2514 3491 659
Size: 751 1104 977 1226
Fragments arranged by size:
1226 1104 977 751
AgeI A'CCGG_T
Cuts at: 2446 2446
Size: 4058
AhdI GACnn_n'nnGTC
Cuts at: 615 835 615
Size: 220 3838
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 169 378 169
Size: 209 3849
AlwNI CAG_nnn'CTG
Cuts at: 1363 2930 1363
Size: 1567 2491
ApaI G_GGCC'C
Cuts at: 556 556
Size: 4058
ApaLI G'TGCA_C
Cuts at: 2828 2828
Size: 4058
ApoI r'AATT_y
Cuts at: 139 634 1062 3411 3667 139
Size: 495 428 2349 256 530
Fragments arranged by size:
2349 530 495 428 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4058
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4058
AvaI C'yCGr_G
Cuts at: 421 441 465 777 1078 421
Size: 20 24 312 301 3401
Fragments arranged by size:
3401 312 301 24 20
BamHI G'GATC_C
Cuts at: 1083 1083
Size: 4058
BanI G'GyrC_C
Cuts at: 390 964 1074 1488 1736 1771 390
Size: 574 110 414 248 35 2677
Fragments arranged by size:
2677 574 414 248 110 35
BanII G_rGCy'C
Cuts at: 193 545 556 869 997 1004 1072 2102 193
Size: 352 11 313 128 7 68 1030 2149
Fragments arranged by size:
2149 1030 352 313 128 68 11 7
BbeI G_GCGC'C
Cuts at: 1492 1740 1492
Size: 248 3810
BbsI GAAGACnn'nnnn_
Cuts at: 614 614
Size: 4058
BbvCI CC'TCA_GC
Cuts at: 919 919
Size: 4058
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 789 823 789
Size: 34 4024
BciVI GTATCCnnnnn_n'
Cuts at: 1956 2717 4058 1956
Size: 761 1341 1956
Fragments arranged by size:
1956 1341 761
BclI T'GATC_A
Cuts at: 1578 1578
Size: 4058
BglI GCCn_nnn'nGGC
Cuts at: 123 123
Size: 4058
BglII A'GATC_T
Cuts at: 1573 3610 1573
Size: 2037 2021
BlpI GC'TnA_GC
Cuts at: 127 1170 127
Size: 1043 3015
Bme1580I G_kGCm'C
Cuts at: 386 393 556 1683 1776 2832 386
Size: 7 163 1127 93 1056 1612
Fragments arranged by size:
1612 1127 1056 163 93 7
BmrI ACTGGGnnnn_n'
Cuts at: 396 1270 1351 1686 3830 396
Size: 874 81 335 2144 624
Fragments arranged by size:
2144 874 624 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2425 2425
Size: 4058
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 207 207
Size: 4058
Bpu10I CC'TnA_GC
Cuts at: 919 919
Size: 4058
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1234 2113 2605 2903 1234
Size: 879 492 298 2389
Fragments arranged by size:
2389 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3762 3762
Size: 4058
BsaAI yAC'GTr
Cuts at: 1411 1411
Size: 4058
BsaBI GATnn'nnATC
Cuts at: 6 1572 3609 6
Size: 1566 2037 455
Fragments arranged by size:
2037 1566 455
BsaHI Gr'CG_yC
Cuts at: 523 1489 1737 2439 3935 523
Size: 966 248 702 1496 646
Fragments arranged by size:
1496 966 702 646 248
BsaWI w'CCGG_w
Cuts at: 1247 1768 2446 2720 2867 3278 1247
Size: 521 678 274 147 411 2027
Fragments arranged by size:
2027 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 856 886 856
Size: 30 4028
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 406 553 406
Size: 147 3911
BseYI C'CCAG_C
Cuts at: 549 1483 2241 2818 3314 549
Size: 934 758 577 496 1293
Fragments arranged by size:
1293 934 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 180 180
Size: 4058
BsiEI CG_ry'CG
Cuts at: 104 1600 1646 2854 104
Size: 1496 46 1208 1308
Fragments arranged by size:
1496 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 193 739 815 1072 1850 2040 2832 193
Size: 546 76 257 778 190 792 1419
Fragments arranged by size:
1419 792 778 546 257 190 76
Bsp1286I G_dGCh'C
Cuts at: 193 386 393 545 556 739 815 869 997
Size: 193 7 152 11 183 76 54 128
Cuts at: 997 1004 1072 1683 1776 1850 2040 2102 2832
Size: 7 68 611 93 74 190 62 730
Cuts at: 2832 193
Size: 1419
Fragments arranged by size:
1419 730 611 193 190 183 152 128
93 76 74 68 62 54 11 7
7
BspEI T'CCGG_A
Cuts at: 3278 3278
Size: 4058
BspHI T'CATG_A
Cuts at: 3234 3234
Size: 4058
BspMI ACCTGCnnnn'nnnn_
Cuts at: 390 672 719 770 794 1108 1322 1624 2005
Size: 282 47 51 24 314 214 302 381
Cuts at: 2005 390
Size: 2443
Fragments arranged by size:
2443 381 314 302 282 214 51 47
24
BsrBI CCG'CTC
Cuts at: 50 182 2350 2404 3888 4053 50
Size: 132 2168 54 1484 165 55
Fragments arranged by size:
2168 1484 165 132 55 54
BsrDI GCAATG_nn'
Cuts at: 899 1970 899
Size: 1071 2987
BsrFI r'CCGG_y
Cuts at: 1022 2056 2237 2446 1022
Size: 1034 181 209 2634
Fragments arranged by size:
2634 1034 209 181
BssSI C'ACGA_G
Cuts at: 2329 2687 2329
Size: 358 3700
BstAPI GCAn_nnn'nTGC
Cuts at: 1435 1435
Size: 4058
BstBI TT'CG_AA
Cuts at: 2419 2419
Size: 4058
BstXI CCAn_nnnn'nTGG
Cuts at: 936 936
Size: 4058
BstYI r'GATC_y
Cuts at: 1 1083 1550 1573 1908 2154 3166 3252 3264
Size: 1082 467 23 335 246 1012 86 12
Cuts at: 3264 3610 1
Size: 346 449
Fragments arranged by size:
1082 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 989 989
Size: 4058
BtgI C'CryG_G
Cuts at: 951 951
Size: 4058
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 485 2008 2189 2352 485
Size: 1523 181 163 2191
Fragments arranged by size:
2191 1523 181 163
BtsI GCAGTG_nn'
Cuts at: 339 510 1301 339
Size: 171 791 3096
Fragments arranged by size:
3096 791 171
DraI TTT'AAA
Cuts at: 1057 1057
Size: 4058
DrdI GACnn_nn'nnGTC
Cuts at: 1764 2622 1764
Size: 858 3200
EaeI y'GGCC_r
Cuts at: 1643 1817 2208 2235 3580 1643
Size: 174 391 27 1345 2121
Fragments arranged by size:
2121 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1643 1643
Size: 4058
EarI CTCTTCn'nnn_
Cuts at: 2081 2291 2081
Size: 210 3848
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2266 2576 2722 3868 2266
Size: 310 146 1146 2456
Fragments arranged by size:
2456 1146 310 146
EcoICRI GAG'CTC
Cuts at: 191 1070 191
Size: 879 3179
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 207 238 1883 2315 3062 3330 207
Size: 31 1645 432 747 268 935
Fragments arranged by size:
1645 935 747 432 268 31
EcoNI CCTnn'n_nnAGG
Cuts at: 204 204
Size: 4058
EcoO109I rG'GnC_Cy
Cuts at: 251 915 1197 251
Size: 664 282 3112
Fragments arranged by size:
3112 664 282
EcoRI G'AATT_C
Cuts at: 139 634 1062 139
Size: 495 428 3135
Fragments arranged by size:
3135 495 428
EcoRV GAT'ATC
Cuts at: 1041 1041
Size: 4058
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2077 2077
Size: 4058
FspI TGC'GCA
Cuts at: 1839 1839
Size: 4058
HaeII r_GCGC'y
Cuts at: 1492 1740 2762 3741 1492
Size: 248 1022 979 1809
Fragments arranged by size:
1809 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 601 633 821 853 1900 1930 1932 1962 601
Size: 32 188 32 1047 30 2 30 2697
Fragments arranged by size:
2697 1047 188 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1097 1097
Size: 4058
KasI G'GCGC_C
Cuts at: 1488 1736 1488
Size: 248 3810
KpnI G_GTAC'C
Cuts at: 1078 1078
Size: 4058
MfeI C'AATT_G
Cuts at: 641 641
Size: 4058
MluI A'CGCG_T
Cuts at: 3491 3491
Size: 4058
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 767 2729 2913 767
Size: 1962 184 1912
Fragments arranged by size:
1962 1912 184
MscI TGG'CCA
Cuts at: 1819 1819
Size: 4058
MslI CAynn'nnrTG
Cuts at: 664 934 2047 2174 664
Size: 270 1113 127 2548
Fragments arranged by size:
2548 1113 270 127
MspA1I CmG'CkG
Cuts at: 368 953 1169 1483 1843 2856 3101 368
Size: 585 216 314 360 1013 245 1325
Fragments arranged by size:
1325 1013 585 360 314 245 216
NaeI GCC'GGC
Cuts at: 2239 2239
Size: 4058
NarI GG'CG_CC
Cuts at: 1489 1737 1489
Size: 248 3810
NgoMIV G'CCGG_C
Cuts at: 2237 2237
Size: 4058
NspI r_CATG'y
Cuts at: 1111 1611 2518 1111
Size: 500 907 2651
Fragments arranged by size:
2651 907 500
PasI CC'CwG_GG
Cuts at: 621 621
Size: 4058
PciI A'CATG_T
Cuts at: 2514 2514
Size: 4058
PmeI GTTT'AAAC
Cuts at: 1057 1057
Size: 4058
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 787 819 3197 3229 787
Size: 32 2378 32 1616
Fragments arranged by size:
2378 1616 32 32
PpuMI rG'GwC_Cy
Cuts at: 251 251
Size: 4058
PshAI GACnn'nnGTC
Cuts at: 700 700
Size: 4058
PspOMI G'GGCC_C
Cuts at: 552 552
Size: 4058
PstI C_TGCA'G
Cuts at: 791 1105 1790 791
Size: 314 685 3059
Fragments arranged by size:
3059 685 314
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4058
PvuII CAG'CTG
Cuts at: 1483 1843 1483
Size: 360 3698
RsrII CG'GwC_CG
Cuts at: 2253 2253
Size: 4058
SacI G_AGCT'C
Cuts at: 193 1072 193
Size: 879 3179
SacII CC_GC'GG
Cuts at: 954 954
Size: 4058
SalI G'TCGA_C
Cuts at: 1095 1095
Size: 4058
SapI GCTCTTCn'nnn_
Cuts at: 2081 2291 2081
Size: 210 3848
SbfI CC_TGCA'GG
Cuts at: 1105 1105
Size: 4058
ScaI AGT'ACT
Cuts at: 267 3575 267
Size: 3308 750
SexAI A'CCwGG_T
Cuts at: 611 611
Size: 4058
SfcI C'TryA_G
Cuts at: 33 787 1101 1786 2779 2970 33
Size: 754 314 685 993 191 1121
Fragments arranged by size:
1121 993 754 685 314 191
SfoI GGC'GCC
Cuts at: 1490 1738 1490
Size: 248 3810
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4058
SmaI CCC'GGG
Cuts at: 423 467 1080 423
Size: 44 613 3401
Fragments arranged by size:
3401 613 44
SmlI C'TyrA_G
Cuts at: 441 1213 2128 2620 2882 441
Size: 772 915 492 262 1617
Fragments arranged by size:
1617 915 772 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4058
SphI G_CATG'C
Cuts at: 1111 1611 1111
Size: 500 3558
StuI AGG'CCT
Cuts at: 290 290
Size: 4058
StyI C'CwwG_G
Cuts at: 1004 1192 1546 1004
Size: 188 354 3516
Fragments arranged by size:
3516 354 188
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 151 185 394 1641 1774 2233 2442 151
Size: 34 209 1247 133 459 209 1767
Fragments arranged by size:
1767 1247 459 209 209 133 34
TatI w'GTAC_w
Cuts at: 265 941 3573 265
Size: 676 2632 750
Fragments arranged by size:
2632 750 676
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 856 888 957 989 3575 3607 856
Size: 32 69 32 2586 32 1307
Fragments arranged by size:
2586 1307 69 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1855 1855
Size: 4058
XbaI T'CTAG_A
Cuts at: 1089 1089
Size: 4058
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1553 1553
Size: 4058
XhoI C'TCGA_G
Cuts at: 441 441
Size: 4058
XmaI C'CCGG_G
Cuts at: 421 465 1078 421
Size: 44 613 3401
Fragments arranged by size:
3401 613 44
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PasI PciI PmeI PpiI PpuMI PshAI
PspOMI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AfeI AflII AleI AloI AscI AvrII BaeI
BfrBI BmgBI BmtI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstZ17I ClaI CspCI DraIII FseI FspAI HindIII
HpaI NcoI NdeI NheI NotI NruI NsiI PacI
PflMI PfoI PmlI PsiI PspXI PsrI SanDI SfiI
SgrAI SnaBI SrfI SspI SwaI XmnI ZraI