Restriction map for
pF1KB6446
(Circular) (Six-base) MAPSORT of: pF1KB6446.seq Check: 4020 from: 1 to: 4154
pF1KB6446 4154 bp
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 157 604 678 157
Size: 447 74 3633
Fragments arranged by size:
3633 447 74
AccI GT'mk_AC
Cuts at: 1192 1192
Size: 4154
Acc65I G'GTAC_C
Cuts at: 1082 1170 1082
Size: 88 4066
AclI AA'CG_TT
Cuts at: 3994 3994
Size: 4154
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 406 1979 2411 3158 3426 406
Size: 1573 432 747 268 1134
Fragments arranged by size:
1573 1134 747 432 268
AflIII A'CryG_T
Cuts at: 1506 2610 3587 1506
Size: 1104 977 2073
Fragments arranged by size:
2073 1104 977
AgeI A'CCGG_T
Cuts at: 2542 2542
Size: 4154
AhdI GACnn_n'nnGTC
Cuts at: 1018 1018
Size: 4154
AleI CACnn'nnGTG
Cuts at: 901 901
Size: 4154
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 955 955
Size: 4154
AlwNI CAG_nnn'CTG
Cuts at: 1459 3026 1459
Size: 1567 2587
ApaLI G'TGCA_C
Cuts at: 2924 2924
Size: 4154
ApoI r'AATT_y
Cuts at: 1158 3507 3763 1158
Size: 2349 256 1549
Fragments arranged by size:
2349 1549 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4154
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4154
AvaI C'yCGr_G
Cuts at: 512 677 1106 1174 512
Size: 165 429 68 3492
Fragments arranged by size:
3492 429 165 68
BamHI G'GATC_C
Cuts at: 1179 1179
Size: 4154
BanI G'GyrC_C
Cuts at: 145 814 892 1082 1170 1584 1832 1867 145
Size: 669 78 190 88 414 248 35 2432
Fragments arranged by size:
2432 669 414 248 190 88 78 35
BanII G_rGCy'C
Cuts at: 332 513 521 684 1168 2198 332
Size: 181 8 163 484 1030 2288
Fragments arranged by size:
2288 1030 484 181 163 8
BbeI G_GCGC'C
Cuts at: 1588 1836 1588
Size: 248 3906
BbsI GAAGACnn'nnnn_
Cuts at: 828 828
Size: 4154
BciVI GTATCCnnnnn_n'
Cuts at: 896 2052 2813 4154 896
Size: 1156 761 1341 896
Fragments arranged by size:
1341 1156 896 761
BclI T'GATC_A
Cuts at: 1674 1674
Size: 4154
BglI GCCn_nnn'nGGC
Cuts at: 217 346 929 217
Size: 129 583 3442
Fragments arranged by size:
3442 583 129
BglII A'GATC_T
Cuts at: 1669 3706 1669
Size: 2037 2117
BlpI GC'TnA_GC
Cuts at: 1266 1266
Size: 4154
Bme1580I G_kGCm'C
Cuts at: 1779 1872 2928 1779
Size: 93 1056 3005
Fragments arranged by size:
3005 1056 93
BmgBI CAC'GTC
Cuts at: 995 995
Size: 4154
BmrI ACTGGGnnnn_n'
Cuts at: 1366 1447 1782 3926 1366
Size: 81 335 2144 1594
Fragments arranged by size:
2144 1594 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2521 2521
Size: 4154
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 271 271
Size: 4154
Bpu10I CC'TnA_GC
Cuts at: 193 193
Size: 4154
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 337 1330 2209 2701 2999 337
Size: 993 879 492 298 1492
Fragments arranged by size:
1492 993 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 196 774 1005 3858 196
Size: 578 231 2853 492
Fragments arranged by size:
2853 578 492 231
BsaAI yAC'GTr
Cuts at: 1507 1507
Size: 4154
BsaBI GATnn'nnATC
Cuts at: 6 1668 3705 6
Size: 1662 2037 455
Fragments arranged by size:
2037 1662 455
BsaHI Gr'CG_yC
Cuts at: 779 1585 1833 2535 4031 779
Size: 806 248 702 1496 902
Fragments arranged by size:
1496 902 806 702 248
BsaWI w'CCGG_w
Cuts at: 1343 1864 2542 2816 2963 3374 1343
Size: 521 678 274 147 411 2123
Fragments arranged by size:
2123 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 464 494 464
Size: 30 4124
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 247 518 521 548 586 1132 247
Size: 271 3 27 38 546 3269
Fragments arranged by size:
3269 546 271 38 27 3
BseYI C'CCAG_C
Cuts at: 207 796 1070 1579 2337 2914 3410 207
Size: 589 274 509 758 577 496 951
Fragments arranged by size:
951 758 589 577 509 496 274
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1041 1041
Size: 4154
BsiEI CG_ry'CG
Cuts at: 104 717 1696 1742 2950 104
Size: 613 979 46 1208 1308
Fragments arranged by size:
1308 1208 979 613 46
BsiHKAI G_wGCw'C
Cuts at: 239 449 513 595 1168 1946 2136 2928 239
Size: 210 64 82 573 778 190 792 1465
Fragments arranged by size:
1465 792 778 573 210 190 82 64
BsmI GAATG_Cn'
Cuts at: 630 651 630
Size: 21 4133
BsmBI CGTCTCn'nnnn_
Cuts at: 979 979
Size: 4154
Bsp1286I G_dGCh'C
Cuts at: 239 332 449 513 521 595 684 1168 1779
Size: 93 117 64 8 74 89 484 611
Cuts at: 1779 1872 1946 2136 2198 2928 239
Size: 93 74 190 62 730 1465
Fragments arranged by size:
1465 730 611 484 190 117 93 93
89 74 74 64 62 8
BspEI T'CCGG_A
Cuts at: 3374 3374
Size: 4154
BspHI T'CATG_A
Cuts at: 3330 3330
Size: 4154
BspMI ACCTGCnnnn'nnnn_
Cuts at: 157 604 678 1204 1418 1720 2101 157
Size: 447 74 526 214 302 381 2210
Fragments arranged by size:
2210 526 447 381 302 214 74
BsrBI CCG'CTC
Cuts at: 50 2446 2500 3984 4149 50
Size: 2396 54 1484 165 55
Fragments arranged by size:
2396 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2066 2066
Size: 4154
BsrFI r'CCGG_y
Cuts at: 647 2152 2333 2542 647
Size: 1505 181 209 2259
Fragments arranged by size:
2259 1505 209 181
BssSI C'ACGA_G
Cuts at: 2425 2783 2425
Size: 358 3796
BstAPI GCAn_nnn'nTGC
Cuts at: 1531 1531
Size: 4154
BstBI TT'CG_AA
Cuts at: 2515 2515
Size: 4154
BstXI CCAn_nnnn'nTGG
Cuts at: 609 636 903 1078 609
Size: 27 267 175 3685
Fragments arranged by size:
3685 267 175 27
BstYI r'GATC_y
Cuts at: 1 617 1179 1646 1669 2004 2250 3262 3348
Size: 616 562 467 23 335 246 1012 86
Cuts at: 3348 3360 3706 1
Size: 12 346 449
Fragments arranged by size:
1012 616 562 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 926 926
Size: 4154
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2104 2285 2448 2104
Size: 181 163 3810
Fragments arranged by size:
3810 181 163
BtsI GCAGTG_nn'
Cuts at: 1397 1397
Size: 4154
DraI TTT'AAA
Cuts at: 1153 1153
Size: 4154
DraIII CAC_nnn'GTG
Cuts at: 243 243
Size: 4154
DrdI GACnn_nn'nnGTC
Cuts at: 1860 2718 1860
Size: 858 3296
EaeI y'GGCC_r
Cuts at: 714 1739 1913 2304 2331 3676 714
Size: 1025 174 391 27 1345 1192
Fragments arranged by size:
1345 1192 1025 391 174 27
EagI C'GGCC_G
Cuts at: 714 1739 714
Size: 1025 3129
EarI CTCTTCn'nnn_
Cuts at: 557 575 2177 2387 557
Size: 18 1602 210 2324
Fragments arranged by size:
2324 1602 210 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 774 2362 2672 2818 3964 774
Size: 1588 310 146 1146 964
Fragments arranged by size:
1588 1146 964 310 146
EcoICRI GAG'CTC
Cuts at: 511 1166 511
Size: 655 3499
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 271 406 1979 2411 3158 3426 271
Size: 135 1573 432 747 268 999
Fragments arranged by size:
1573 999 747 432 268 135
EcoNI CCTnn'n_nnAGG
Cuts at: 568 568
Size: 4154
EcoO109I rG'GnC_Cy
Cuts at: 304 1066 1293 304
Size: 762 227 3165
Fragments arranged by size:
3165 762 227
EcoRI G'AATT_C
Cuts at: 1158 1158
Size: 4154
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2173 2173
Size: 4154
FspI TGC'GCA
Cuts at: 1935 1935
Size: 4154
HaeII r_GCGC'y
Cuts at: 1588 1836 2858 3837 1588
Size: 248 1022 979 1905
Fragments arranged by size:
1905 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1004 1036 1996 2026 2028 2058 1004
Size: 32 960 30 2 30 3100
Fragments arranged by size:
3100 960 32 30 30 2
HincII GTy'rAC
Cuts at: 1193 1193
Size: 4154
KasI G'GCGC_C
Cuts at: 1584 1832 1584
Size: 248 3906
KpnI G_GTAC'C
Cuts at: 1086 1174 1086
Size: 88 4066
MluI A'CGCG_T
Cuts at: 3587 3587
Size: 4154
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2825 3009 2825
Size: 184 3970
MscI TGG'CCA
Cuts at: 1915 1915
Size: 4154
MslI CAynn'nnrTG
Cuts at: 901 997 2143 2270 901
Size: 96 1146 127 2785
Fragments arranged by size:
2785 1146 127 96
MspA1I CmG'CkG
Cuts at: 732 1055 1074 1265 1579 1939 2952 3197 732
Size: 323 19 191 314 360 1013 245 1689
Fragments arranged by size:
1689 1013 360 323 314 245 191 19
NaeI GCC'GGC
Cuts at: 649 2335 649
Size: 1686 2468
NarI GG'CG_CC
Cuts at: 1585 1833 1585
Size: 248 3906
NgoMIV G'CCGG_C
Cuts at: 647 2333 647
Size: 1686 2468
NotI GC'GGCC_GC
Cuts at: 714 714
Size: 4154
NspI r_CATG'y
Cuts at: 1207 1707 2614 1207
Size: 500 907 2747
Fragments arranged by size:
2747 907 500
PciI A'CATG_T
Cuts at: 2610 2610
Size: 4154
PfoI T'CCnGG_A
Cuts at: 914 914
Size: 4154
PmeI GTTT'AAAC
Cuts at: 1153 1153
Size: 4154
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3293 3325 3293
Size: 32 4122
PpuMI rG'GwC_Cy
Cuts at: 304 1066 304
Size: 762 3392
PshAI GACnn'nnGTC
Cuts at: 971 971
Size: 4154
PstI C_TGCA'G
Cuts at: 689 1201 1886 689
Size: 512 685 2957
Fragments arranged by size:
2957 685 512
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4154
PvuII CAG'CTG
Cuts at: 732 1055 1074 1579 1939 732
Size: 323 19 505 360 2947
Fragments arranged by size:
2947 505 360 323 19
RsrII CG'GwC_CG
Cuts at: 2349 2349
Size: 4154
SacI G_AGCT'C
Cuts at: 513 1168 513
Size: 655 3499
SalI G'TCGA_C
Cuts at: 1191 1191
Size: 4154
SanDI GG'GwC_CC
Cuts at: 1066 1066
Size: 4154
SapI GCTCTTCn'nnn_
Cuts at: 575 2177 2387 575
Size: 1602 210 2342
Fragments arranged by size:
2342 1602 210
SbfI CC_TGCA'GG
Cuts at: 1201 1201
Size: 4154
ScaI AGT'ACT
Cuts at: 3671 3671
Size: 4154
SfcI C'TryA_G
Cuts at: 33 685 1197 1882 2875 3066 33
Size: 652 512 685 993 191 1121
Fragments arranged by size:
1121 993 685 652 512 191
SfoI GGC'GCC
Cuts at: 1586 1834 1586
Size: 248 3906
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4154
SmaI CCC'GGG
Cuts at: 679 1108 1176 679
Size: 429 68 3657
Fragments arranged by size:
3657 429 68
SmlI C'TyrA_G
Cuts at: 352 1309 2224 2716 2978 352
Size: 957 915 492 262 1528
Fragments arranged by size:
1528 957 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4154
SphI G_CATG'C
Cuts at: 1207 1707 1207
Size: 500 3654
StuI AGG'CCT
Cuts at: 700 700
Size: 4154
StyI C'CwwG_G
Cuts at: 1288 1642 1288
Size: 354 3800
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 881 1029 1737 1870 2329 2538 881
Size: 148 708 133 459 209 2497
Fragments arranged by size:
2497 708 459 209 148 133
TatI w'GTAC_w
Cuts at: 3669 3669
Size: 4154
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 229 261 464 496 3671 3703 229
Size: 32 203 32 3175 32 680
Fragments arranged by size:
3175 680 203 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 168 1951 168
Size: 1783 2371
XbaI T'CTAG_A
Cuts at: 1185 1185
Size: 4154
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 751 1649 751
Size: 898 3256
XmaI C'CCGG_G
Cuts at: 677 1106 1174 677
Size: 429 68 3657
Fragments arranged by size:
3657 429 68
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NotI NspI PciI PfoI PmeI PpiI PpuMI PshAI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AloI ApaI AscI AvrII BaeI
BbvCI BcgI BfrBI BmtI BsiWI BsrGI BssHII BstEII
BstZ17I BtgI ClaI CspCI EcoRV FseI FspAI HindIII
HpaI MfeI NcoI NdeI NheI NruI NsiI PacI
PasI PflMI PmlI PsiI PspOMI PspXI PsrI SacII
SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
XmnI ZraI