Restriction map for
pF1KB6448
(Circular) (Six-base) MAPSORT of: pF1KB6448.seq Check: 2316 from: 1 to: 4160
pF1KB6448 4160 bp
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 705 705
Size: 4160
AccI GT'mk_AC
Cuts at: 1198 1198
Size: 4160
Acc65I G'GTAC_C
Cuts at: 1176 1176
Size: 4160
AclI AA'CG_TT
Cuts at: 1154 4000 1154
Size: 2846 1314
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1985 2417 3164 3432 1985
Size: 432 747 268 2713
Fragments arranged by size:
2713 747 432 268
AflIII A'CryG_T
Cuts at: 1512 2616 3593 1512
Size: 1104 977 2079
Fragments arranged by size:
2079 1104 977
AgeI A'CCGG_T
Cuts at: 2548 2548
Size: 4160
AleI CACnn'nnGTG
Cuts at: 812 812
Size: 4160
AlwNI CAG_nnn'CTG
Cuts at: 1465 3032 1465
Size: 1567 2593
ApaI G_GGCC'C
Cuts at: 1121 1121
Size: 4160
ApaLI G'TGCA_C
Cuts at: 2930 2930
Size: 4160
ApoI r'AATT_y
Cuts at: 478 1164 3513 3769 478
Size: 686 2349 256 869
Fragments arranged by size:
2349 869 686 256
AseI AT'TA_AT
Cuts at: 20 617 20
Size: 597 3563
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4160
AvaI C'yCGr_G
Cuts at: 155 257 1180 155
Size: 102 923 3135
Fragments arranged by size:
3135 923 102
BamHI G'GATC_C
Cuts at: 1185 1185
Size: 4160
BanI G'GyrC_C
Cuts at: 1176 1590 1838 1873 1176
Size: 414 248 35 3463
Fragments arranged by size:
3463 414 248 35
BanII G_rGCy'C
Cuts at: 155 174 1008 1121 1174 2204 155
Size: 19 834 113 53 1030 2111
Fragments arranged by size:
2111 1030 834 113 53 19
BbeI G_GCGC'C
Cuts at: 1594 1842 1594
Size: 248 3912
BciVI GTATCCnnnnn_n'
Cuts at: 329 2058 2819 4160 329
Size: 1729 761 1341 329
Fragments arranged by size:
1729 1341 761 329
BclI T'GATC_A
Cuts at: 1136 1680 1136
Size: 544 3616
BfrBI ATG'CAT
Cuts at: 755 755
Size: 4160
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 4160
BglII A'GATC_T
Cuts at: 1675 3712 1675
Size: 2037 2123
BlpI GC'TnA_GC
Cuts at: 1272 1272
Size: 4160
Bme1580I G_kGCm'C
Cuts at: 1121 1785 1878 2934 1121
Size: 664 93 1056 2347
Fragments arranged by size:
2347 1056 664 93
BmrI ACTGGGnnnn_n'
Cuts at: 192 1372 1453 1788 3932 192
Size: 1180 81 335 2144 420
Fragments arranged by size:
2144 1180 420 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2527 2527
Size: 4160
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 167 188 167
Size: 21 4139
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1336 2215 2707 3005 1336
Size: 879 492 298 2491
Fragments arranged by size:
2491 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3864 3864
Size: 4160
BsaAI yAC'GTr
Cuts at: 1513 1513
Size: 4160
BsaBI GATnn'nnATC
Cuts at: 6 976 1674 3711 6
Size: 970 698 2037 455
Fragments arranged by size:
2037 970 698 455
BsaHI Gr'CG_yC
Cuts at: 1591 1839 2541 4037 1591
Size: 248 702 1496 1714
Fragments arranged by size:
1714 1496 702 248
BsaWI w'CCGG_w
Cuts at: 768 1349 1870 2548 2822 2969 3380 768
Size: 581 521 678 274 147 411 1548
Fragments arranged by size:
1548 678 581 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 175 280 298 536 175
Size: 105 18 238 3799
Fragments arranged by size:
3799 238 105 18
BseYI C'CCAG_C
Cuts at: 641 1585 2343 2920 3416 641
Size: 944 758 577 496 1385
Fragments arranged by size:
1385 944 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1702 1748 2956 104
Size: 1598 46 1208 1308
Fragments arranged by size:
1598 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1174 1952 2142 2934 1174
Size: 778 190 792 2400
Fragments arranged by size:
2400 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 155 174 1008 1121 1174 1785 1878 1952 2142
Size: 19 834 113 53 611 93 74 190
Cuts at: 2142 2204 2934 155
Size: 62 730 1381
Fragments arranged by size:
1381 834 730 611 190 113 93 74
62 53 19
BspEI T'CCGG_A
Cuts at: 768 3380 768
Size: 2612 1548
BspHI T'CATG_A
Cuts at: 483 3336 483
Size: 2853 1307
BspMI ACCTGCnnnn'nnnn_
Cuts at: 356 671 705 1210 1424 1726 2107 356
Size: 315 34 505 214 302 381 2409
Fragments arranged by size:
2409 505 381 315 302 214 34
BsrBI CCG'CTC
Cuts at: 50 2452 2506 3990 4155 50
Size: 2402 54 1484 165 55
Fragments arranged by size:
2402 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 372 2072 372
Size: 1700 2460
BsrFI r'CCGG_y
Cuts at: 2158 2339 2548 2158
Size: 181 209 3770
Fragments arranged by size:
3770 209 181
BsrGI T'GTAC_A
Cuts at: 383 383
Size: 4160
BssSI C'ACGA_G
Cuts at: 2431 2789 2431
Size: 358 3802
BstAPI GCAn_nnn'nTGC
Cuts at: 1537 1537
Size: 4160
BstBI TT'CG_AA
Cuts at: 2521 2521
Size: 4160
BstXI CCAn_nnnn'nTGG
Cuts at: 935 935
Size: 4160
BstYI r'GATC_y
Cuts at: 1 1185 1652 1675 2010 2256 3268 3354 3366
Size: 1184 467 23 335 246 1012 86 12
Cuts at: 3366 3712 1
Size: 346 449
Fragments arranged by size:
1184 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4160
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2110 2291 2454 2110
Size: 181 163 3816
Fragments arranged by size:
3816 181 163
BtsI GCAGTG_nn'
Cuts at: 1403 1403
Size: 4160
DraI TTT'AAA
Cuts at: 1159 1159
Size: 4160
DrdI GACnn_nn'nnGTC
Cuts at: 1866 2724 1866
Size: 858 3302
EaeI y'GGCC_r
Cuts at: 431 1055 1745 1919 2310 2337 3682 431
Size: 624 690 174 391 27 1345 909
Fragments arranged by size:
1345 909 690 624 391 174 27
EagI C'GGCC_G
Cuts at: 1745 1745
Size: 4160
EarI CTCTTCn'nnn_
Cuts at: 524 2183 2393 524
Size: 1659 210 2291
Fragments arranged by size:
2291 1659 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 139 2368 2678 2824 3970 139
Size: 2229 310 146 1146 329
Fragments arranged by size:
2229 1146 329 310 146
EcoICRI GAG'CTC
Cuts at: 1172 1172
Size: 4160
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 167 188 1985 2417 3164 3432 167
Size: 21 1797 432 747 268 895
Fragments arranged by size:
1797 895 747 432 268 21
EcoO109I rG'GnC_Cy
Cuts at: 336 1117 1118 1299 336
Size: 781 1 181 3197
Fragments arranged by size:
3197 781 181 1
EcoRI G'AATT_C
Cuts at: 1164 1164
Size: 4160
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2179 2179
Size: 4160
FspI TGC'GCA
Cuts at: 205 1941 205
Size: 1736 2424
HaeII r_GCGC'y
Cuts at: 1594 1842 2864 3843 1594
Size: 248 1022 979 1911
Fragments arranged by size:
1911 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 525 557 684 716 2002 2032 2034 2064 525
Size: 32 127 32 1286 30 2 30 2621
Fragments arranged by size:
2621 1286 127 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1199 1199
Size: 4160
KasI G'GCGC_C
Cuts at: 1590 1838 1590
Size: 248 3912
KpnI G_GTAC'C
Cuts at: 1180 1180
Size: 4160
MluI A'CGCG_T
Cuts at: 3593 3593
Size: 4160
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 485 2831 3015 485
Size: 2346 184 1630
Fragments arranged by size:
2346 1630 184
MscI TGG'CCA
Cuts at: 433 1057 1921 433
Size: 624 864 2672
Fragments arranged by size:
2672 864 624
MslI CAynn'nnrTG
Cuts at: 676 707 812 928 982 2149 2276 676
Size: 31 105 116 54 1167 127 2560
Fragments arranged by size:
2560 1167 127 116 105 54 31
MspA1I CmG'CkG
Cuts at: 121 1271 1585 1945 2958 3203 121
Size: 1150 314 360 1013 245 1078
Fragments arranged by size:
1150 1078 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2341 2341
Size: 4160
NarI GG'CG_CC
Cuts at: 1591 1839 1591
Size: 248 3912
NcoI C'CATG_G
Cuts at: 107 107
Size: 4160
NgoMIV G'CCGG_C
Cuts at: 2339 2339
Size: 4160
NsiI A_TGCA'T
Cuts at: 757 757
Size: 4160
NspI r_CATG'y
Cuts at: 1213 1713 2620 1213
Size: 500 907 2753
Fragments arranged by size:
2753 907 500
PciI A'CATG_T
Cuts at: 2616 2616
Size: 4160
PflMI CCAn_nnn'nTGG
Cuts at: 879 879
Size: 4160
PmeI GTTT'AAAC
Cuts at: 1159 1159
Size: 4160
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3299 3331 3299
Size: 32 4128
PpuMI rG'GwC_Cy
Cuts at: 336 336
Size: 4160
PspOMI G'GGCC_C
Cuts at: 1117 1117
Size: 4160
PstI C_TGCA'G
Cuts at: 506 548 1207 1892 506
Size: 42 659 685 2774
Fragments arranged by size:
2774 685 659 42
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4160
PvuII CAG'CTG
Cuts at: 1585 1945 1585
Size: 360 3800
RsrII CG'GwC_CG
Cuts at: 2355 2355
Size: 4160
SacI G_AGCT'C
Cuts at: 1174 1174
Size: 4160
SalI G'TCGA_C
Cuts at: 1197 1197
Size: 4160
SapI GCTCTTCn'nnn_
Cuts at: 2183 2393 2183
Size: 210 3950
SbfI CC_TGCA'GG
Cuts at: 1207 1207
Size: 4160
ScaI AGT'ACT
Cuts at: 3677 3677
Size: 4160
SfcI C'TryA_G
Cuts at: 33 502 544 1203 1888 2881 3072 33
Size: 469 42 659 685 993 191 1121
Fragments arranged by size:
1121 993 685 659 469 191 42
SfoI GGC'GCC
Cuts at: 1592 1840 1592
Size: 248 3912
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4160
SmaI CCC'GGG
Cuts at: 157 259 1182 157
Size: 102 923 3135
Fragments arranged by size:
3135 923 102
SmlI C'TyrA_G
Cuts at: 1315 2230 2722 2984 1315
Size: 915 492 262 2491
Fragments arranged by size:
2491 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4160
SphI G_CATG'C
Cuts at: 1213 1713 1213
Size: 500 3660
SspI AAT'ATT
Cuts at: 360 360
Size: 4160
StuI AGG'CCT
Cuts at: 1093 1093
Size: 4160
StyI C'CwwG_G
Cuts at: 107 1094 1294 1648 107
Size: 987 200 354 2619
Fragments arranged by size:
2619 987 354 200
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 316 412 824 1743 1876 2335 2544 316
Size: 96 412 919 133 459 209 1932
Fragments arranged by size:
1932 919 459 412 209 133 96
TatI w'GTAC_w
Cuts at: 383 410 932 3675 383
Size: 27 522 2743 868
Fragments arranged by size:
2743 868 522 27
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3677 3709 3677
Size: 32 4128
Tth111I GACn'n_nGTC
Cuts at: 1957 1957
Size: 4160
XbaI T'CTAG_A
Cuts at: 1191 1191
Size: 4160
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 880 935 1655 880
Size: 55 720 3385
Fragments arranged by size:
3385 720 55
XmaI C'CCGG_G
Cuts at: 155 257 1180 155
Size: 102 923 3135
Fragments arranged by size:
3135 923 102
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NsiI NspI PciI PflMI PmeI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI AscI AvrII
BaeI BbsI BbvCI BcgI BmgBI BmtI Bpu10I BsaXI
BsgI BsiWI BsmI BsmBI BssHII BstEII BstZ17I Bsu36I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI PacI PasI
PfoI PmlI PshAI PsiI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SwaI XhoI XmnI
ZraI