Restriction map for
pF1KB6449
(Circular) (Six-base) MAPSORT of: pF1KB6449.seq Check: 9298 from: 1 to: 4064
pF1KB6449 4064 bp
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AccI GT'mk_AC
Cuts at: 666 1102 666
Size: 436 3628
Acc65I G'GTAC_C
Cuts at: 1080 1080
Size: 4064
AclI AA'CG_TT
Cuts at: 3904 3904
Size: 4064
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 187 655 922 1889 2321 3068 3336 187
Size: 468 267 967 432 747 268 915
Fragments arranged by size:
967 915 747 468 432 268 267
AflIII A'CryG_T
Cuts at: 659 661 1416 2520 3497 659
Size: 2 755 1104 977 1226
Fragments arranged by size:
1226 1104 977 755 2
AgeI A'CCGG_T
Cuts at: 2452 2452
Size: 4064
AhdI GACnn_n'nnGTC
Cuts at: 804 981 804
Size: 177 3887
AlwNI CAG_nnn'CTG
Cuts at: 912 945 1050 1369 2936 912
Size: 33 105 319 1567 2040
Fragments arranged by size:
2040 1567 319 105 33
ApaI G_GGCC'C
Cuts at: 154 154
Size: 4064
ApaLI G'TGCA_C
Cuts at: 2834 2834
Size: 4064
ApoI r'AATT_y
Cuts at: 1068 3417 3673 1068
Size: 2349 256 1459
Fragments arranged by size:
2349 1459 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4064
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4064
AvaI C'yCGr_G
Cuts at: 268 1084 268
Size: 816 3248
BamHI G'GATC_C
Cuts at: 1089 1089
Size: 4064
BanI G'GyrC_C
Cuts at: 282 1080 1494 1742 1777 282
Size: 798 414 248 35 2569
Fragments arranged by size:
2569 798 414 248 35
BanII G_rGCy'C
Cuts at: 154 302 524 749 1078 2108 154
Size: 148 222 225 329 1030 2110
Fragments arranged by size:
2110 1030 329 225 222 148
BbeI G_GCGC'C
Cuts at: 1498 1746 1498
Size: 248 3816
BbvCI CC'TCA_GC
Cuts at: 488 488
Size: 4064
BciVI GTATCCnnnnn_n'
Cuts at: 893 1962 2723 4064 893
Size: 1069 761 1341 893
Fragments arranged by size:
1341 1069 893 761
BclI T'GATC_A
Cuts at: 464 1584 464
Size: 1120 2944
BglII A'GATC_T
Cuts at: 1579 3616 1579
Size: 2037 2027
BlpI GC'TnA_GC
Cuts at: 1176 1176
Size: 4064
Bme1580I G_kGCm'C
Cuts at: 154 1689 1782 2838 154
Size: 1535 93 1056 1380
Fragments arranged by size:
1535 1380 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1276 1357 1692 3836 1276
Size: 81 335 2144 1504
Fragments arranged by size:
2144 1504 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2431 2431
Size: 4064
Bpu10I CC'TnA_GC
Cuts at: 488 518 740 488
Size: 30 222 3812
Fragments arranged by size:
3812 222 30
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1240 2119 2611 2909 1240
Size: 879 492 298 2395
Fragments arranged by size:
2395 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 398 3768 398
Size: 3370 694
BsaAI yAC'GTr
Cuts at: 662 1417 662
Size: 755 3309
BsaBI GATnn'nnATC
Cuts at: 6 1578 3615 6
Size: 1572 2037 455
Fragments arranged by size:
2037 1572 455
BsaHI Gr'CG_yC
Cuts at: 1495 1743 2445 3941 1495
Size: 248 702 1496 1618
Fragments arranged by size:
1618 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1253 1774 2452 2726 2873 3284 1253
Size: 521 678 274 147 411 2033
Fragments arranged by size:
2033 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 610 640 610
Size: 30 4034
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 241 979 241
Size: 738 3326
BseYI C'CCAG_C
Cuts at: 912 1489 2247 2824 3320 912
Size: 577 758 577 496 1656
Fragments arranged by size:
1656 758 577 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 325 591 325
Size: 266 3798
BsiEI CG_ry'CG
Cuts at: 104 122 192 1606 1652 2860 104
Size: 18 70 1414 46 1208 1308
Fragments arranged by size:
1414 1308 1208 70 46 18
BsiHKAI G_wGCw'C
Cuts at: 524 1078 1856 2046 2838 524
Size: 554 778 190 792 1750
Fragments arranged by size:
1750 792 778 554 190
Bsp1286I G_dGCh'C
Cuts at: 154 302 524 749 1078 1689 1782 1856 2046
Size: 148 222 225 329 611 93 74 190
Cuts at: 2046 2108 2838 154
Size: 62 730 1380
Fragments arranged by size:
1380 730 611 329 225 222 190 148
93 74 62
BspEI T'CCGG_A
Cuts at: 3284 3284
Size: 4064
BspHI T'CATG_A
Cuts at: 3240 3240
Size: 4064
BspMI ACCTGCnnnn'nnnn_
Cuts at: 443 794 1114 1328 1630 2011 443
Size: 351 320 214 302 381 2496
Fragments arranged by size:
2496 381 351 320 302 214
BsrBI CCG'CTC
Cuts at: 50 177 2356 2410 3894 4059 50
Size: 127 2179 54 1484 165 55
Fragments arranged by size:
2179 1484 165 127 55 54
BsrDI GCAATG_nn'
Cuts at: 1976 1976
Size: 4064
BsrFI r'CCGG_y
Cuts at: 2062 2243 2452 2062
Size: 181 209 3674
Fragments arranged by size:
3674 209 181
BssSI C'ACGA_G
Cuts at: 2335 2693 2335
Size: 358 3706
BstAPI GCAn_nnn'nTGC
Cuts at: 282 1441 282
Size: 1159 2905
BstBI TT'CG_AA
Cuts at: 2425 2425
Size: 4064
BstYI r'GATC_y
Cuts at: 1 260 1089 1556 1579 1914 2160 3172 3258
Size: 259 829 467 23 335 246 1012 86
Cuts at: 3258 3270 3616 1
Size: 12 346 449
Fragments arranged by size:
1012 829 467 449 346 335 259 246
86 23 12
BtgI C'CryG_G
Cuts at: 143 377 684 143
Size: 234 307 3523
Fragments arranged by size:
3523 307 234
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2014 2195 2358 2014
Size: 181 163 3720
Fragments arranged by size:
3720 181 163
BtsI GCAGTG_nn'
Cuts at: 785 1307 785
Size: 522 3542
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 437 472 437
Size: 35 4029
DraI TTT'AAA
Cuts at: 1063 1063
Size: 4064
DrdI GACnn_nn'nnGTC
Cuts at: 1770 2628 1770
Size: 858 3206
EaeI y'GGCC_r
Cuts at: 119 347 578 654 756 1649 1823 2214 2241
Size: 228 231 76 102 893 174 391 27
Cuts at: 2241 3586 119
Size: 1345 597
Fragments arranged by size:
1345 893 597 391 231 228 174 102
76 27
EagI C'GGCC_G
Cuts at: 119 1649 119
Size: 1530 2534
EarI CTCTTCn'nnn_
Cuts at: 2087 2297 2087
Size: 210 3854
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 253 379 2272 2582 2728 3874 253
Size: 126 1893 310 146 1146 443
Fragments arranged by size:
1893 1146 443 310 146 126
EcoICRI GAG'CTC
Cuts at: 522 1076 522
Size: 554 3510
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 187 655 922 1889 2321 3068 3336 187
Size: 468 267 967 432 747 268 915
Fragments arranged by size:
967 915 747 468 432 268 267
EcoO109I rG'GnC_Cy
Cuts at: 150 151 1203 150
Size: 1 1052 3011
Fragments arranged by size:
3011 1052 1
EcoRI G'AATT_C
Cuts at: 1068 1068
Size: 4064
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2083 2083
Size: 4064
FspI TGC'GCA
Cuts at: 1845 1845
Size: 4064
HaeII r_GCGC'y
Cuts at: 129 1498 1746 2768 3747 129
Size: 1369 248 1022 979 446
Fragments arranged by size:
1369 1022 979 446 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 316 348 790 822 967 999 1906 1936 1938
Size: 32 442 32 145 32 907 30 2
Cuts at: 1938 1968 316
Size: 30 2412
Fragments arranged by size:
2412 907 442 145 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 837 1103 837
Size: 266 3798
KasI G'GCGC_C
Cuts at: 1494 1742 1494
Size: 248 3816
KpnI G_GTAC'C
Cuts at: 1084 1084
Size: 4064
MluI A'CGCG_T
Cuts at: 3497 3497
Size: 4064
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2735 2919 2735
Size: 184 3880
MscI TGG'CCA
Cuts at: 349 580 656 1825 349
Size: 231 76 1169 2588
Fragments arranged by size:
2588 1169 231 76
MslI CAynn'nnrTG
Cuts at: 382 2053 2180 382
Size: 1671 127 2266
Fragments arranged by size:
2266 1671 127
MspA1I CmG'CkG
Cuts at: 203 912 921 1175 1489 1849 2862 3107 203
Size: 709 9 254 314 360 1013 245 1160
Fragments arranged by size:
1160 1013 709 360 314 254 245 9
NaeI GCC'GGC
Cuts at: 2245 2245
Size: 4064
NarI GG'CG_CC
Cuts at: 1495 1743 1495
Size: 248 3816
NcoI C'CATG_G
Cuts at: 143 377 684 143
Size: 234 307 3523
Fragments arranged by size:
3523 307 234
NgoMIV G'CCGG_C
Cuts at: 2243 2243
Size: 4064
NspI r_CATG'y
Cuts at: 1117 1617 2524 1117
Size: 500 907 2657
Fragments arranged by size:
2657 907 500
PciI A'CATG_T
Cuts at: 2520 2520
Size: 4064
PmeI GTTT'AAAC
Cuts at: 1063 1063
Size: 4064
PmlI CAC'GTG
Cuts at: 662 662
Size: 4064
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 973 1005 3203 3235 973
Size: 32 2198 32 1802
Fragments arranged by size:
2198 1802 32 32
PspOMI G'GGCC_C
Cuts at: 150 150
Size: 4064
PspXI vC'TCGA_Gb
Cuts at: 268 268
Size: 4064
PstI C_TGCA'G
Cuts at: 257 440 1111 1796 257
Size: 183 671 685 2525
Fragments arranged by size:
2525 685 671 183
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4064
PvuII CAG'CTG
Cuts at: 912 921 1489 1849 912
Size: 9 568 360 3127
Fragments arranged by size:
3127 568 360 9
RsrII CG'GwC_CG
Cuts at: 2259 2259
Size: 4064
SacI G_AGCT'C
Cuts at: 524 1078 524
Size: 554 3510
SalI G'TCGA_C
Cuts at: 1101 1101
Size: 4064
SapI GCTCTTCn'nnn_
Cuts at: 2087 2297 2087
Size: 210 3854
SbfI CC_TGCA'GG
Cuts at: 1111 1111
Size: 4064
ScaI AGT'ACT
Cuts at: 3581 3581
Size: 4064
SexAI A'CCwGG_T
Cuts at: 800 800
Size: 4064
SfcI C'TryA_G
Cuts at: 33 253 436 1107 1792 2785 2976 33
Size: 220 183 671 685 993 191 1121
Fragments arranged by size:
1121 993 685 671 220 191 183
SfoI GGC'GCC
Cuts at: 1496 1744 1496
Size: 248 3816
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4064
SmaI CCC'GGG
Cuts at: 1086 1086
Size: 4064
SmlI C'TyrA_G
Cuts at: 268 1219 2134 2626 2888 268
Size: 951 915 492 262 1444
Fragments arranged by size:
1444 951 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4064
SphI G_CATG'C
Cuts at: 1117 1617 1117
Size: 500 3564
StyI C'CwwG_G
Cuts at: 143 377 684 719 1198 1552 143
Size: 234 307 35 479 354 2655
Fragments arranged by size:
2655 479 354 307 234 35
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 712 1647 1780 2239 2448 712
Size: 935 133 459 209 2328
Fragments arranged by size:
2328 935 459 209 133
TatI w'GTAC_w
Cuts at: 3579 3579
Size: 4064
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 610 642 3581 3613 610
Size: 32 2939 32 1061
Fragments arranged by size:
2939 1061 32 32
Tth111I GACn'n_nGTC
Cuts at: 600 1861 600
Size: 1261 2803
XbaI T'CTAG_A
Cuts at: 1095 1095
Size: 4064
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1559 1559
Size: 4064
XhoI C'TCGA_G
Cuts at: 268 268
Size: 4064
XmaI C'CCGG_G
Cuts at: 1084 1084
Size: 4064
XmnI GAAnn'nnTTC
Cuts at: 935 935
Size: 4064
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BciVI BclI BglII BlpI Bme1580I
BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PmeI PmlI PpiI PspOMI PspXI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI AscI
AvrII BaeI BbsI BcgI BfrBI BglI BmgBI BmtI
BpmI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PfoI PpuMI PshAI PsiI PsrI
SacII SanDI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI ZraI