Restriction map for
pF1KB6450
(Circular) (Six-base) MAPSORT of: pF1KB6450.seq Check: 445 from: 1 to: 4160
pF1KB6450 4160 bp
With 182 enzymes: SgfI *
October 24, 2019 20:49 ..
AccI GT'mk_AC
Cuts at: 1198 1198
Size: 4160
Acc65I G'GTAC_C
Cuts at: 1176 1176
Size: 4160
AclI AA'CG_TT
Cuts at: 4000 4000
Size: 4160
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1985 2417 3164 3432 1985
Size: 432 747 268 2713
Fragments arranged by size:
2713 747 432 268
AfeI AGC'GCT
Cuts at: 803 803
Size: 4160
AflIII A'CryG_T
Cuts at: 1512 2616 3593 1512
Size: 1104 977 2079
Fragments arranged by size:
2079 1104 977
AgeI A'CCGG_T
Cuts at: 2548 2548
Size: 4160
AleI CACnn'nnGTG
Cuts at: 488 488
Size: 4160
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 749 781 749
Size: 32 4128
AlwNI CAG_nnn'CTG
Cuts at: 1465 3032 1465
Size: 1567 2593
ApaLI G'TGCA_C
Cuts at: 2930 2930
Size: 4160
ApoI r'AATT_y
Cuts at: 577 605 1164 3513 3769 577
Size: 28 559 2349 256 968
Fragments arranged by size:
2349 968 559 256 28
AseI AT'TA_AT
Cuts at: 20 508 20
Size: 488 3672
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4160
AvaI C'yCGr_G
Cuts at: 776 842 848 1180 776
Size: 66 6 332 3756
Fragments arranged by size:
3756 332 66 6
BamHI G'GATC_C
Cuts at: 159 1185 159
Size: 1026 3134
BanI G'GyrC_C
Cuts at: 730 855 879 1098 1176 1590 1838 1873 730
Size: 125 24 219 78 414 248 35 3017
Fragments arranged by size:
3017 414 248 219 125 78 35 24
BanII G_rGCy'C
Cuts at: 849 1174 2204 849
Size: 325 1030 2805
Fragments arranged by size:
2805 1030 325
BbeI G_GCGC'C
Cuts at: 1594 1842 1594
Size: 248 3912
BbsI GAAGACnn'nnnn_
Cuts at: 242 242
Size: 4160
BciVI GTATCCnnnnn_n'
Cuts at: 329 865 1120 2058 2819 4160 329
Size: 536 255 938 761 1341 329
Fragments arranged by size:
1341 938 761 536 329 255
BclI T'GATC_A
Cuts at: 1680 1680
Size: 4160
BfrBI ATG'CAT
Cuts at: 559 559
Size: 4160
BglI GCCn_nnn'nGGC
Cuts at: 853 853
Size: 4160
BglII A'GATC_T
Cuts at: 1675 3712 1675
Size: 2037 2123
BlpI GC'TnA_GC
Cuts at: 1272 1272
Size: 4160
Bme1580I G_kGCm'C
Cuts at: 858 882 1785 1878 2934 858
Size: 24 903 93 1056 2084
Fragments arranged by size:
2084 1056 903 93 24
BmrI ACTGGGnnnn_n'
Cuts at: 1372 1453 1788 3932 1372
Size: 81 335 2144 1600
Fragments arranged by size:
2144 1600 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 784 2527 784
Size: 1743 2417
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 875 875
Size: 4160
Bpu10I CC'TnA_GC
Cuts at: 131 440 131
Size: 309 3851
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 507 1336 2215 2707 3005 507
Size: 829 879 492 298 1662
Fragments arranged by size:
1662 879 829 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3864 3864
Size: 4160
BsaAI yAC'GTr
Cuts at: 547 625 1513 547
Size: 78 888 3194
Fragments arranged by size:
3194 888 78
BsaBI GATnn'nnATC
Cuts at: 6 1674 3711 6
Size: 1668 2037 455
Fragments arranged by size:
2037 1668 455
BsaHI Gr'CG_yC
Cuts at: 1591 1839 2541 4037 1591
Size: 248 702 1496 1714
Fragments arranged by size:
1714 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1349 1870 2548 2822 2969 3380 1349
Size: 521 678 274 147 411 2129
Fragments arranged by size:
2129 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 130 731 875 878 130
Size: 601 144 3 3412
Fragments arranged by size:
3412 601 144 3
BseYI C'CCAG_C
Cuts at: 1585 2343 2920 3416 1585
Size: 758 577 496 2329
Fragments arranged by size:
2329 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1702 1748 2956 104
Size: 1598 46 1208 1308
Fragments arranged by size:
1598 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1174 1952 2142 2934 1174
Size: 778 190 792 2400
Fragments arranged by size:
2400 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 849 858 882 1174 1785 1878 1952 2142 2204
Size: 9 24 292 611 93 74 190 62
Cuts at: 2204 2934 849
Size: 730 2075
Fragments arranged by size:
2075 730 611 292 190 93 74 62
24 9
BspEI T'CCGG_A
Cuts at: 3380 3380
Size: 4160
BspHI T'CATG_A
Cuts at: 3336 3336
Size: 4160
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1210 1424 1726 2107 1210
Size: 214 302 381 3263
Fragments arranged by size:
3263 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2452 2506 3990 4155 50
Size: 2402 54 1484 165 55
Fragments arranged by size:
2402 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2072 2072
Size: 4160
BsrFI r'CCGG_y
Cuts at: 2158 2339 2548 2158
Size: 181 209 3770
Fragments arranged by size:
3770 209 181
BssSI C'ACGA_G
Cuts at: 2431 2789 2431
Size: 358 3802
BstAPI GCAn_nnn'nTGC
Cuts at: 1537 1537
Size: 4160
BstBI TT'CG_AA
Cuts at: 2521 2521
Size: 4160
BstXI CCAn_nnnn'nTGG
Cuts at: 1070 1070
Size: 4160
BstYI r'GATC_y
Cuts at: 1 159 1185 1652 1675 2010 2256 3268 3354
Size: 158 1026 467 23 335 246 1012 86
Cuts at: 3354 3366 3712 1
Size: 12 346 449
Fragments arranged by size:
1026 1012 467 449 346 335 246 158
86 23 12
BtgI C'CryG_G
Cuts at: 107 723 795 107
Size: 616 72 3472
Fragments arranged by size:
3472 616 72
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2110 2291 2454 2110
Size: 181 163 3816
Fragments arranged by size:
3816 181 163
BtsI GCAGTG_nn'
Cuts at: 1403 1403
Size: 4160
DraI TTT'AAA
Cuts at: 1159 1159
Size: 4160
DraIII CAC_nnn'GTG
Cuts at: 1000 1000
Size: 4160
DrdI GACnn_nn'nnGTC
Cuts at: 1866 2724 1866
Size: 858 3302
EaeI y'GGCC_r
Cuts at: 1745 1919 2310 2337 3682 1745
Size: 174 391 27 1345 2223
Fragments arranged by size:
2223 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1745 1745
Size: 4160
EarI CTCTTCn'nnn_
Cuts at: 107 367 454 560 2183 2393 107
Size: 260 87 106 1623 210 1874
Fragments arranged by size:
1874 1623 260 210 106 87
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2368 2678 2824 3970 2368
Size: 310 146 1146 2558
Fragments arranged by size:
2558 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1172 1172
Size: 4160
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 875 1985 2417 3164 3432 875
Size: 1110 432 747 268 1603
Fragments arranged by size:
1603 1110 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 372 758 372
Size: 386 3774
EcoO109I rG'GnC_Cy
Cuts at: 1299 1299
Size: 4160
EcoRI G'AATT_C
Cuts at: 1164 1164
Size: 4160
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2179 2179
Size: 4160
FspI TGC'GCA
Cuts at: 1941 1941
Size: 4160
HaeII r_GCGC'y
Cuts at: 805 1594 1842 2864 3843 805
Size: 789 248 1022 979 1122
Fragments arranged by size:
1122 1022 979 789 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2002 2032 2034 2064 2002
Size: 30 2 30 4098
Fragments arranged by size:
4098 30 30 2
HincII GTy'rAC
Cuts at: 1199 1199
Size: 4160
HindIII A'AGCT_T
Cuts at: 536 902 536
Size: 366 3794
KasI G'GCGC_C
Cuts at: 1590 1838 1590
Size: 248 3912
KpnI G_GTAC'C
Cuts at: 1180 1180
Size: 4160
MluI A'CGCG_T
Cuts at: 3593 3593
Size: 4160
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2831 3015 2831
Size: 184 3976
MscI TGG'CCA
Cuts at: 1921 1921
Size: 4160
MslI CAynn'nnrTG
Cuts at: 488 1142 2149 2276 488
Size: 654 1007 127 2372
Fragments arranged by size:
2372 1007 654 127
MspA1I CmG'CkG
Cuts at: 1271 1585 1945 2958 3203 1271
Size: 314 360 1013 245 2228
Fragments arranged by size:
2228 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2341 2341
Size: 4160
NarI GG'CG_CC
Cuts at: 1591 1839 1591
Size: 248 3912
NcoI C'CATG_G
Cuts at: 107 107
Size: 4160
NgoMIV G'CCGG_C
Cuts at: 2339 2339
Size: 4160
NsiI A_TGCA'T
Cuts at: 561 561
Size: 4160
NspI r_CATG'y
Cuts at: 178 1213 1713 2620 178
Size: 1035 500 907 1718
Fragments arranged by size:
1718 1035 907 500
PciI A'CATG_T
Cuts at: 2616 2616
Size: 4160
PflMI CCAn_nnn'nTGG
Cuts at: 754 754
Size: 4160
PmeI GTTT'AAAC
Cuts at: 1159 1159
Size: 4160
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3299 3331 3299
Size: 32 4128
PshAI GACnn'nnGTC
Cuts at: 659 1022 659
Size: 363 3797
PspXI vC'TCGA_Gb
Cuts at: 842 842
Size: 4160
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 310 342 310
Size: 32 4128
PstI C_TGCA'G
Cuts at: 1207 1892 1207
Size: 685 3475
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4160
PvuII CAG'CTG
Cuts at: 1585 1945 1585
Size: 360 3800
RsrII CG'GwC_CG
Cuts at: 2355 2355
Size: 4160
SacI G_AGCT'C
Cuts at: 1174 1174
Size: 4160
SalI G'TCGA_C
Cuts at: 1197 1197
Size: 4160
SapI GCTCTTCn'nnn_
Cuts at: 2183 2393 2183
Size: 210 3950
SbfI CC_TGCA'GG
Cuts at: 1207 1207
Size: 4160
ScaI AGT'ACT
Cuts at: 299 3677 299
Size: 3378 782
SfcI C'TryA_G
Cuts at: 33 379 818 1203 1888 2881 3072 33
Size: 346 439 385 685 993 191 1121
Fragments arranged by size:
1121 993 685 439 385 346 191
SfoI GGC'GCC
Cuts at: 1592 1840 1592
Size: 248 3912
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4160
SmaI CCC'GGG
Cuts at: 850 1182 850
Size: 332 3828
SmlI C'TyrA_G
Cuts at: 486 842 1315 2230 2722 2984 486
Size: 356 473 915 492 262 1662
Fragments arranged by size:
1662 915 492 473 356 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4160
SphI G_CATG'C
Cuts at: 178 1213 1713 178
Size: 1035 500 2625
Fragments arranged by size:
2625 1035 500
SspI AAT'ATT
Cuts at: 124 124
Size: 4160
StyI C'CwwG_G
Cuts at: 107 1294 1648 107
Size: 1187 354 2619
Fragments arranged by size:
2619 1187 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1743 1876 2335 2544 1743
Size: 133 459 209 3359
Fragments arranged by size:
3359 459 209 133
TatI w'GTAC_w
Cuts at: 297 3675 297
Size: 3378 782
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3677 3709 3677
Size: 32 4128
Tth111I GACn'n_nGTC
Cuts at: 1957 1957
Size: 4160
XbaI T'CTAG_A
Cuts at: 1191 1191
Size: 4160
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1655 1655
Size: 4160
XhoI C'TCGA_G
Cuts at: 842 842
Size: 4160
XmaI C'CCGG_G
Cuts at: 848 1180 848
Size: 332 3828
XmnI GAAnn'nnTTC
Cuts at: 209 209
Size: 4160
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PciI
PflMI PmeI PpiI PshAI PspXI PsrI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AlfI ApaI AscI AvrII
BaeI BbvCI BcgI BmgBI BmtI BsaXI BsgI BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI
CspCI EcoRV FseI FspAI HpaI MfeI NdeI NheI
NotI NruI PacI PasI PfoI PmlI PpuMI PsiI
PspOMI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
StuI SwaI ZraI