Restriction map for
pF1KB6452
(Circular) (Six-base) MAPSORT of: pF1KB6452.seq Check: 8705 from: 1 to: 4163
pF1KB6452 4163 bp
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AccI GT'mk_AC
Cuts at: 143 1201 143
Size: 1058 3105
Acc65I G'GTAC_C
Cuts at: 1179 1179
Size: 4163
AclI AA'CG_TT
Cuts at: 4003 4003
Size: 4163
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 340 904 1988 2420 3167 3435 340
Size: 564 1084 432 747 268 1068
Fragments arranged by size:
1084 1068 747 564 432 268
AflIII A'CryG_T
Cuts at: 752 1515 2619 3596 752
Size: 763 1104 977 1319
Fragments arranged by size:
1319 1104 977 763
AgeI A'CCGG_T
Cuts at: 2551 2551
Size: 4163
AhdI GACnn_n'nnGTC
Cuts at: 423 985 423
Size: 562 3601
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 791 823 791
Size: 32 4131
AlwNI CAG_nnn'CTG
Cuts at: 492 1047 1468 3035 492
Size: 555 421 1567 1620
Fragments arranged by size:
1620 1567 555 421
ApaI G_GGCC'C
Cuts at: 138 138
Size: 4163
ApaLI G'TGCA_C
Cuts at: 2933 2933
Size: 4163
ApoI r'AATT_y
Cuts at: 649 1167 3516 3772 649
Size: 518 2349 256 1040
Fragments arranged by size:
2349 1040 518 256
AseI AT'TA_AT
Cuts at: 20 281 20
Size: 261 3902
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4163
AvaI C'yCGr_G
Cuts at: 137 201 1183 137
Size: 64 982 3117
Fragments arranged by size:
3117 982 64
BamHI G'GATC_C
Cuts at: 1188 1188
Size: 4163
BanI G'GyrC_C
Cuts at: 1179 1593 1841 1876 1179
Size: 414 248 35 3466
Fragments arranged by size:
3466 414 248 35
BanII G_rGCy'C
Cuts at: 138 716 1177 2207 138
Size: 578 461 1030 2094
Fragments arranged by size:
2094 1030 578 461
BbeI G_GCGC'C
Cuts at: 1597 1845 1597
Size: 248 3915
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4129
BciVI GTATCCnnnnn_n'
Cuts at: 2061 2822 4163 2061
Size: 761 1341 2061
Fragments arranged by size:
2061 1341 761
BclI T'GATC_A
Cuts at: 1683 1683
Size: 4163
BglI GCCn_nnn'nGGC
Cuts at: 1060 1060
Size: 4163
BglII A'GATC_T
Cuts at: 1005 1678 3715 1005
Size: 673 2037 1453
Fragments arranged by size:
2037 1453 673
BlpI GC'TnA_GC
Cuts at: 1275 1275
Size: 4163
Bme1580I G_kGCm'C
Cuts at: 138 1788 1881 2937 138
Size: 1650 93 1056 1364
Fragments arranged by size:
1650 1364 1056 93
BmgBI CAC'GTC
Cuts at: 198 198
Size: 4163
BmrI ACTGGGnnnn_n'
Cuts at: 189 1375 1456 1791 3935 189
Size: 1186 81 335 2144 417
Fragments arranged by size:
2144 1186 417 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2530 2530
Size: 4163
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 385 385
Size: 4163
Bpu10I CC'TnA_GC
Cuts at: 665 665
Size: 4163
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 675 1339 2218 2710 3008 675
Size: 664 879 492 298 1830
Fragments arranged by size:
1830 879 664 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1147 3867 1147
Size: 2720 1443
BsaAI yAC'GTr
Cuts at: 1516 1516
Size: 4163
BsaBI GATnn'nnATC
Cuts at: 6 1677 3714 6
Size: 1671 2037 455
Fragments arranged by size:
2037 1671 455
BsaHI Gr'CG_yC
Cuts at: 1594 1842 2544 4040 1594
Size: 248 702 1496 1717
Fragments arranged by size:
1717 1496 702 248
BsaWI w'CCGG_w
Cuts at: 509 1352 1873 2551 2825 2972 3383 509
Size: 843 521 678 274 147 411 1289
Fragments arranged by size:
1289 843 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 698 728 698
Size: 30 4133
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 180 706 180
Size: 526 3637
BseYI C'CCAG_C
Cuts at: 206 950 1106 1588 2346 2923 3419 206
Size: 744 156 482 758 577 496 950
Fragments arranged by size:
950 758 744 577 496 482 156
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1136 1136
Size: 4163
BsiEI CG_ry'CG
Cuts at: 104 122 1705 1751 2959 104
Size: 18 1583 46 1208 1308
Fragments arranged by size:
1583 1308 1208 46 18
BsiHKAI G_wGCw'C
Cuts at: 716 1073 1177 1955 2145 2937 716
Size: 357 104 778 190 792 1942
Fragments arranged by size:
1942 792 778 357 190 104
Bsp1286I G_dGCh'C
Cuts at: 138 716 1073 1177 1788 1881 1955 2145 2207
Size: 578 357 104 611 93 74 190 62
Cuts at: 2207 2937 138
Size: 730 1364
Fragments arranged by size:
1364 730 611 578 357 190 104 93
74 62
BspEI T'CCGG_A
Cuts at: 3383 3383
Size: 4163
BspHI T'CATG_A
Cuts at: 596 3339 596
Size: 2743 1420
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1213 1427 1729 2110 1213
Size: 214 302 381 3266
Fragments arranged by size:
3266 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2455 2509 3993 4158 50
Size: 2405 54 1484 165 55
Fragments arranged by size:
2405 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 842 2075 842
Size: 1233 2930
BsrFI r'CCGG_y
Cuts at: 2161 2342 2551 2161
Size: 181 209 3773
Fragments arranged by size:
3773 209 181
BssSI C'ACGA_G
Cuts at: 718 1154 2434 2792 718
Size: 436 1280 358 2089
Fragments arranged by size:
2089 1280 436 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1123 1540 1123
Size: 417 3746
BstBI TT'CG_AA
Cuts at: 237 792 2524 237
Size: 555 1732 1876
Fragments arranged by size:
1876 1732 555
BstXI CCAn_nnnn'nTGG
Cuts at: 855 855
Size: 4163
BstYI r'GATC_y
Cuts at: 1 488 906 1005 1188 1655 1678 2013 2259
Size: 487 418 99 183 467 23 335 246
Cuts at: 2259 3271 3357 3369 3715 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 487 467 449 418 346 335 246
183 99 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 995 995
Size: 4163
BtgI C'CryG_G
Cuts at: 122 810 1064 122
Size: 688 254 3221
Fragments arranged by size:
3221 688 254
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2113 2294 2457 2113
Size: 181 163 3819
Fragments arranged by size:
3819 181 163
BtsI GCAGTG_nn'
Cuts at: 1406 1406
Size: 4163
DraI TTT'AAA
Cuts at: 1162 1162
Size: 4163
DrdI GACnn_nn'nnGTC
Cuts at: 1869 2727 1869
Size: 858 3305
EaeI y'GGCC_r
Cuts at: 119 1748 1922 2313 2340 3685 119
Size: 1629 174 391 27 1345 597
Fragments arranged by size:
1629 1345 597 391 174 27
EagI C'GGCC_G
Cuts at: 119 1748 119
Size: 1629 2534
EarI CTCTTCn'nnn_
Cuts at: 2186 2396 2186
Size: 210 3953
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2371 2681 2827 3973 2371
Size: 310 146 1146 2561
Fragments arranged by size:
2561 1146 310 146
EcoICRI GAG'CTC
Cuts at: 714 1175 714
Size: 461 3702
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 340 385 904 1988 2420 3167 3435 340
Size: 45 519 1084 432 747 268 1068
Fragments arranged by size:
1084 1068 747 519 432 268 45
EcoO109I rG'GnC_Cy
Cuts at: 134 417 999 1302 134
Size: 283 582 303 2995
Fragments arranged by size:
2995 582 303 283
EcoRI G'AATT_C
Cuts at: 649 1167 649
Size: 518 3645
EcoRV GAT'ATC
Cuts at: 507 924 507
Size: 417 3746
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2182 2182
Size: 4163
FspI TGC'GCA
Cuts at: 1944 1944
Size: 4163
HaeII r_GCGC'y
Cuts at: 1597 1845 2867 3846 1597
Size: 248 1022 979 1914
Fragments arranged by size:
1914 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 409 441 698 730 971 1003 2005 2035 2037
Size: 32 257 32 241 32 1002 30 2
Cuts at: 2037 2067 409
Size: 30 2505
Fragments arranged by size:
2505 1002 257 241 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1202 1202
Size: 4163
KasI G'GCGC_C
Cuts at: 1593 1841 1593
Size: 248 3915
KpnI G_GTAC'C
Cuts at: 1183 1183
Size: 4163
MluI A'CGCG_T
Cuts at: 3596 3596
Size: 4163
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2834 3018 2834
Size: 184 3979
MscI TGG'CCA
Cuts at: 1924 1924
Size: 4163
MslI CAynn'nnrTG
Cuts at: 575 2152 2279 575
Size: 1577 127 2459
Fragments arranged by size:
2459 1577 127
MspA1I CmG'CkG
Cuts at: 124 1274 1588 1948 2961 3206 124
Size: 1150 314 360 1013 245 1081
Fragments arranged by size:
1150 1081 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2344 2344
Size: 4163
NarI GG'CG_CC
Cuts at: 1594 1842 1594
Size: 248 3915
NcoI C'CATG_G
Cuts at: 810 1064 810
Size: 254 3909
NgoMIV G'CCGG_C
Cuts at: 2342 2342
Size: 4163
NspI r_CATG'y
Cuts at: 756 1216 1716 2623 756
Size: 460 500 907 2296
Fragments arranged by size:
2296 907 500 460
PciI A'CATG_T
Cuts at: 752 2619 752
Size: 1867 2296
PmeI GTTT'AAAC
Cuts at: 1162 1162
Size: 4163
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3302 3334 3302
Size: 32 4131
PpuMI rG'GwC_Cy
Cuts at: 417 417
Size: 4163
PspOMI G'GGCC_C
Cuts at: 134 134
Size: 4163
PstI C_TGCA'G
Cuts at: 1210 1895 1210
Size: 685 3478
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4163
PvuII CAG'CTG
Cuts at: 1588 1948 1588
Size: 360 3803
RsrII CG'GwC_CG
Cuts at: 2358 2358
Size: 4163
SacI G_AGCT'C
Cuts at: 716 1177 716
Size: 461 3702
SacII CC_GC'GG
Cuts at: 125 125
Size: 4163
SalI G'TCGA_C
Cuts at: 1200 1200
Size: 4163
SapI GCTCTTCn'nnn_
Cuts at: 2186 2396 2186
Size: 210 3953
SbfI CC_TGCA'GG
Cuts at: 1210 1210
Size: 4163
ScaI AGT'ACT
Cuts at: 178 3680 178
Size: 3502 661
SfcI C'TryA_G
Cuts at: 33 1206 1891 2884 3075 33
Size: 1173 685 993 191 1121
Fragments arranged by size:
1173 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1595 1843 1595
Size: 248 3915
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4163
SmaI CCC'GGG
Cuts at: 139 1185 139
Size: 1046 3117
SmlI C'TyrA_G
Cuts at: 690 1318 2233 2725 2987 690
Size: 628 915 492 262 1866
Fragments arranged by size:
1866 915 628 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4163
SphI G_CATG'C
Cuts at: 1216 1716 1216
Size: 500 3663
StyI C'CwwG_G
Cuts at: 810 848 1064 1297 1651 810
Size: 38 216 233 354 3322
Fragments arranged by size:
3322 354 233 216 38
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1088 1746 1879 2338 2547 1088
Size: 658 133 459 209 2704
Fragments arranged by size:
2704 658 459 209 133
TatI w'GTAC_w
Cuts at: 176 671 3678 176
Size: 495 3007 661
Fragments arranged by size:
3007 661 495
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3680 3712 3680
Size: 32 4131
Tth111I GACn'n_nGTC
Cuts at: 1960 1960
Size: 4163
XbaI T'CTAG_A
Cuts at: 1194 1194
Size: 4163
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1062 1658 1062
Size: 596 3567
XmaI C'CCGG_G
Cuts at: 137 1183 137
Size: 1046 3117
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AscI AvrII
BaeI BbsI BbvCI BfrBI BmtI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstZ17I ClaI CspCI DraIII EcoNI
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmlI PshAI
PsiI PspXI PsrI SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI