Restriction map for
pF1KB6455
(Circular) (Six-base) MAPSORT of: pF1KB6455.seq Check: 1876 from: 1 to: 4067
pF1KB6455 4067 bp
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 454 454
Size: 4067
AccI GT'mk_AC
Cuts at: 266 533 1105 266
Size: 267 572 3228
Fragments arranged by size:
3228 572 267
Acc65I G'GTAC_C
Cuts at: 1083 1083
Size: 4067
AclI AA'CG_TT
Cuts at: 3907 3907
Size: 4067
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 111 332 1892 2324 3071 3339 111
Size: 221 1560 432 747 268 839
Fragments arranged by size:
1560 839 747 432 268 221
AflIII A'CryG_T
Cuts at: 1419 2523 3500 1419
Size: 1104 977 1986
Fragments arranged by size:
1986 1104 977
AgeI A'CCGG_T
Cuts at: 2455 2455
Size: 4067
AlwNI CAG_nnn'CTG
Cuts at: 1372 2939 1372
Size: 1567 2500
ApaLI G'TGCA_C
Cuts at: 2837 2837
Size: 4067
ApoI r'AATT_y
Cuts at: 208 506 1071 3420 3676 208
Size: 298 565 2349 256 599
Fragments arranged by size:
2349 599 565 298 256
AseI AT'TA_AT
Cuts at: 20 329 20
Size: 309 3758
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4067
AvaI C'yCGr_G
Cuts at: 1087 1087
Size: 4067
BamHI G'GATC_C
Cuts at: 1092 1092
Size: 4067
BanI G'GyrC_C
Cuts at: 420 826 1083 1497 1745 1780 420
Size: 406 257 414 248 35 2707
Fragments arranged by size:
2707 414 406 257 248 35
BanII G_rGCy'C
Cuts at: 1081 2111 1081
Size: 1030 3037
BbeI G_GCGC'C
Cuts at: 424 830 1501 1749 424
Size: 406 671 248 2742
Fragments arranged by size:
2742 671 406 248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4033
BciVI GTATCCnnnnn_n'
Cuts at: 1965 2726 4067 1965
Size: 761 1341 1965
Fragments arranged by size:
1965 1341 761
BclI T'GATC_A
Cuts at: 320 1587 320
Size: 1267 2800
BglII A'GATC_T
Cuts at: 1582 3619 1582
Size: 2037 2030
BlpI GC'TnA_GC
Cuts at: 1179 1179
Size: 4067
Bme1580I G_kGCm'C
Cuts at: 1692 1785 2841 1692
Size: 93 1056 2918
Fragments arranged by size:
2918 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 523 1279 1360 1695 3839 523
Size: 756 81 335 2144 751
Fragments arranged by size:
2144 756 751 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 600 2434 600
Size: 1834 2233
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 202 202
Size: 4067
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1243 2122 2614 2912 1243
Size: 879 492 298 2398
Fragments arranged by size:
2398 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 217 3771 217
Size: 3554 513
BsaAI yAC'GTr
Cuts at: 253 1420 253
Size: 1167 2900
BsaBI GATnn'nnATC
Cuts at: 6 979 1581 3618 6
Size: 973 602 2037 455
Fragments arranged by size:
2037 973 602 455
BsaHI Gr'CG_yC
Cuts at: 421 827 1498 1746 2448 3944 421
Size: 406 671 248 702 1496 544
Fragments arranged by size:
1496 702 671 544 406 248
BsaWI w'CCGG_w
Cuts at: 868 1256 1777 2455 2729 2876 3287 868
Size: 388 521 678 274 147 411 1648
Fragments arranged by size:
1648 678 521 411 388 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 248 278 1016 1046 248
Size: 30 738 30 3269
Fragments arranged by size:
3269 738 30 30
BseYI C'CCAG_C
Cuts at: 1492 2250 2827 3323 1492
Size: 758 577 496 2236
Fragments arranged by size:
2236 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 488 585 488
Size: 97 3970
BsiEI CG_ry'CG
Cuts at: 104 1609 1655 2863 104
Size: 1505 46 1208 1308
Fragments arranged by size:
1505 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1081 1859 2049 2841 1081
Size: 778 190 792 2307
Fragments arranged by size:
2307 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 1081 1692 1785 1859 2049 2111 2841 1081
Size: 611 93 74 190 62 730 2307
Fragments arranged by size:
2307 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3287 3287
Size: 4067
BspHI T'CATG_A
Cuts at: 323 3243 323
Size: 2920 1147
BspMI ACCTGCnnnn'nnnn_
Cuts at: 454 1117 1331 1633 2014 454
Size: 663 214 302 381 2507
Fragments arranged by size:
2507 663 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2359 2413 3897 4062 50
Size: 2309 54 1484 165 55
Fragments arranged by size:
2309 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 647 954 1979 647
Size: 307 1025 2735
Fragments arranged by size:
2735 1025 307
BsrFI r'CCGG_y
Cuts at: 552 2065 2246 2455 552
Size: 1513 181 209 2164
Fragments arranged by size:
2164 1513 209 181
BssSI C'ACGA_G
Cuts at: 2338 2696 2338
Size: 358 3709
BstAPI GCAn_nnn'nTGC
Cuts at: 794 1444 794
Size: 650 3417
BstBI TT'CG_AA
Cuts at: 2428 2428
Size: 4067
BstXI CCAn_nnnn'nTGG
Cuts at: 711 762 711
Size: 51 4016
BstYI r'GATC_y
Cuts at: 1 1092 1559 1582 1917 2163 3175 3261 3273
Size: 1091 467 23 335 246 1012 86 12
Cuts at: 3273 3619 1
Size: 346 449
Fragments arranged by size:
1091 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 935 935
Size: 4067
BtgI C'CryG_G
Cuts at: 485 485
Size: 4067
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2017 2198 2361 2017
Size: 181 163 3723
Fragments arranged by size:
3723 181 163
BtsI GCAGTG_nn'
Cuts at: 1310 1310
Size: 4067
DraI TTT'AAA
Cuts at: 1066 1066
Size: 4067
DrdI GACnn_nn'nnGTC
Cuts at: 1773 2631 1773
Size: 858 3209
EaeI y'GGCC_r
Cuts at: 752 1652 1826 2217 2244 3589 752
Size: 900 174 391 27 1345 1230
Fragments arranged by size:
1345 1230 900 391 174 27
EagI C'GGCC_G
Cuts at: 1652 1652
Size: 4067
EarI CTCTTCn'nnn_
Cuts at: 2090 2300 2090
Size: 210 3857
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2275 2585 2731 3877 2275
Size: 310 146 1146 2465
Fragments arranged by size:
2465 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1079 1079
Size: 4067
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 111 202 332 1892 2324 3071 3339 111
Size: 91 130 1560 432 747 268 839
Fragments arranged by size:
1560 839 747 432 268 130 91
EcoO109I rG'GnC_Cy
Cuts at: 1206 1206
Size: 4067
EcoRI G'AATT_C
Cuts at: 1071 1071
Size: 4067
EcoRV GAT'ATC
Cuts at: 525 525
Size: 4067
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2086 2086
Size: 4067
FspI TGC'GCA
Cuts at: 1848 1848
Size: 4067
HaeII r_GCGC'y
Cuts at: 424 830 1501 1749 2771 3750 424
Size: 406 671 248 1022 979 741
Fragments arranged by size:
1022 979 741 671 406 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 248 280 1909 1939 1941 1971 248
Size: 32 1629 30 2 30 2344
Fragments arranged by size:
2344 1629 32 30 30 2
HincII GTy'rAC
Cuts at: 1106 1106
Size: 4067
HindIII A'AGCT_T
Cuts at: 436 436
Size: 4067
KasI G'GCGC_C
Cuts at: 420 826 1497 1745 420
Size: 406 671 248 2742
Fragments arranged by size:
2742 671 406 248
KpnI G_GTAC'C
Cuts at: 1087 1087
Size: 4067
MluI A'CGCG_T
Cuts at: 3500 3500
Size: 4067
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2738 2922 2738
Size: 184 3883
MscI TGG'CCA
Cuts at: 754 1828 754
Size: 1074 2993
MslI CAynn'nnrTG
Cuts at: 760 985 2056 2183 760
Size: 225 1071 127 2644
Fragments arranged by size:
2644 1071 225 127
MspA1I CmG'CkG
Cuts at: 131 805 823 1178 1492 1852 2865 3110 131
Size: 674 18 355 314 360 1013 245 1088
Fragments arranged by size:
1088 1013 674 360 355 314 245 18
NaeI GCC'GGC
Cuts at: 554 2248 554
Size: 1694 2373
NarI GG'CG_CC
Cuts at: 421 827 1498 1746 421
Size: 406 671 248 2742
Fragments arranged by size:
2742 671 406 248
NcoI C'CATG_G
Cuts at: 485 485
Size: 4067
NgoMIV G'CCGG_C
Cuts at: 552 2246 552
Size: 1694 2373
NspI r_CATG'y
Cuts at: 499 902 1120 1620 2527 499
Size: 403 218 500 907 2039
Fragments arranged by size:
2039 907 500 403 218
PciI A'CATG_T
Cuts at: 2523 2523
Size: 4067
PmeI GTTT'AAAC
Cuts at: 1066 1066
Size: 4067
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3206 3238 3206
Size: 32 4035
PstI C_TGCA'G
Cuts at: 369 1114 1799 369
Size: 745 685 2637
Fragments arranged by size:
2637 745 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4067
PvuII CAG'CTG
Cuts at: 805 823 1492 1852 805
Size: 18 669 360 3020
Fragments arranged by size:
3020 669 360 18
RsrII CG'GwC_CG
Cuts at: 2262 2262
Size: 4067
SacI G_AGCT'C
Cuts at: 1081 1081
Size: 4067
SalI G'TCGA_C
Cuts at: 1104 1104
Size: 4067
SapI GCTCTTCn'nnn_
Cuts at: 2090 2300 2090
Size: 210 3857
SbfI CC_TGCA'GG
Cuts at: 1114 1114
Size: 4067
ScaI AGT'ACT
Cuts at: 3584 3584
Size: 4067
SfcI C'TryA_G
Cuts at: 33 365 458 1110 1795 2788 2979 33
Size: 332 93 652 685 993 191 1121
Fragments arranged by size:
1121 993 685 652 332 191 93
SfoI GGC'GCC
Cuts at: 422 828 1499 1747 422
Size: 406 671 248 2742
Fragments arranged by size:
2742 671 406 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4067
SmaI CCC'GGG
Cuts at: 1089 1089
Size: 4067
SmlI C'TyrA_G
Cuts at: 1222 2137 2629 2891 1222
Size: 915 492 262 2398
Fragments arranged by size:
2398 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4067
SphI G_CATG'C
Cuts at: 902 1120 1620 902
Size: 218 500 3349
Fragments arranged by size:
3349 500 218
StyI C'CwwG_G
Cuts at: 485 1201 1555 485
Size: 716 354 2997
Fragments arranged by size:
2997 716 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1650 1783 2242 2451 1650
Size: 133 459 209 3266
Fragments arranged by size:
3266 459 209 133
TatI w'GTAC_w
Cuts at: 3582 3582
Size: 4067
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3584 3616 3584
Size: 32 4035
Tth111I GACn'n_nGTC
Cuts at: 1864 1864
Size: 4067
XbaI T'CTAG_A
Cuts at: 1098 1098
Size: 4067
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1562 1562
Size: 4067
XmaI C'CCGG_G
Cuts at: 1087 1087
Size: 4067
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BcgI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PmeI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BfrBI BglI BmgBI
BmtI Bpu10I BseRI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstZ17I ClaI CspCI DraIII EcoNI FseI FspAI
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI