Restriction map for
pF1KB6456
(Circular) (Six-base) MAPSORT of: pF1KB6456.seq Check: 5434 from: 1 to: 4166
pF1KB6456 4166 bp
With 182 enzymes: SgfI *
October 7, 2009 14:20 ..
AccI GT'mk_AC
Cuts at: 314 1204 314
Size: 890 3276
Acc65I G'GTAC_C
Cuts at: 1182 1182
Size: 4166
AclI AA'CG_TT
Cuts at: 4006 4006
Size: 4166
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 149 830 1991 2423 3170 3438 149
Size: 681 1161 432 747 268 877
Fragments arranged by size:
1161 877 747 681 432 268
AfeI AGC'GCT
Cuts at: 436 436
Size: 4166
AflIII A'CryG_T
Cuts at: 1518 2622 3599 1518
Size: 1104 977 2085
Fragments arranged by size:
2085 1104 977
AgeI A'CCGG_T
Cuts at: 2554 2554
Size: 4166
AlwNI CAG_nnn'CTG
Cuts at: 1471 3038 1471
Size: 1567 2599
ApaLI G'TGCA_C
Cuts at: 2936 2936
Size: 4166
ApoI r'AATT_y
Cuts at: 423 488 565 1071 1170 3519 3775 423
Size: 65 77 506 99 2349 256 814
Fragments arranged by size:
2349 814 506 256 99 77 65
AseI AT'TA_AT
Cuts at: 20 20
Size: 4166
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4166
AvaI C'yCGr_G
Cuts at: 1186 1186
Size: 4166
BamHI G'GATC_C
Cuts at: 1191 1191
Size: 4166
BanI G'GyrC_C
Cuts at: 1182 1596 1844 1879 1182
Size: 414 248 35 3469
Fragments arranged by size:
3469 414 248 35
BanII G_rGCy'C
Cuts at: 1180 2210 1180
Size: 1030 3136
BbeI G_GCGC'C
Cuts at: 1600 1848 1600
Size: 248 3918
BbsI GAAGACnn'nnnn_
Cuts at: 1131 1131
Size: 4166
BciVI GTATCCnnnnn_n'
Cuts at: 2064 2825 4166 2064
Size: 761 1341 2064
Fragments arranged by size:
2064 1341 761
BclI T'GATC_A
Cuts at: 1686 1686
Size: 4166
BglII A'GATC_T
Cuts at: 1681 3718 1681
Size: 2037 2129
BlpI GC'TnA_GC
Cuts at: 1278 1278
Size: 4166
Bme1580I G_kGCm'C
Cuts at: 1791 1884 2940 1791
Size: 93 1056 3017
Fragments arranged by size:
3017 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1378 1459 1794 3938 1378
Size: 81 335 2144 1606
Fragments arranged by size:
2144 1606 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2533 2533
Size: 4166
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1342 2221 2713 3011 1342
Size: 879 492 298 2497
Fragments arranged by size:
2497 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3870 3870
Size: 4166
BsaAI yAC'GTr
Cuts at: 1519 1519
Size: 4166
BsaBI GATnn'nnATC
Cuts at: 6 1680 3717 6
Size: 1674 2037 455
Fragments arranged by size:
2037 1674 455
BsaHI Gr'CG_yC
Cuts at: 1597 1845 2547 4043 1597
Size: 248 702 1496 1720
Fragments arranged by size:
1720 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1355 1876 2554 2828 2975 3386 1355
Size: 521 678 274 147 411 2135
Fragments arranged by size:
2135 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1591 2349 2926 3422 1591
Size: 758 577 496 2335
Fragments arranged by size:
2335 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 472 472
Size: 4166
BsiEI CG_ry'CG
Cuts at: 104 137 1708 1754 2962 104
Size: 33 1571 46 1208 1308
Fragments arranged by size:
1571 1308 1208 46 33
BsiHKAI G_wGCw'C
Cuts at: 1180 1958 2148 2940 1180
Size: 778 190 792 2406
Fragments arranged by size:
2406 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 1180 1791 1884 1958 2148 2210 2940 1180
Size: 611 93 74 190 62 730 2406
Fragments arranged by size:
2406 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3386 3386
Size: 4166
BspHI T'CATG_A
Cuts at: 3342 3342
Size: 4166
BspMI ACCTGCnnnn'nnnn_
Cuts at: 444 1216 1430 1732 2113 444
Size: 772 214 302 381 2497
Fragments arranged by size:
2497 772 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2458 2512 3996 4161 50
Size: 2408 54 1484 165 55
Fragments arranged by size:
2408 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2078 2078
Size: 4166
BsrFI r'CCGG_y
Cuts at: 227 2164 2345 2554 227
Size: 1937 181 209 1839
Fragments arranged by size:
1937 1839 209 181
BsrGI T'GTAC_A
Cuts at: 518 518
Size: 4166
BssSI C'ACGA_G
Cuts at: 2437 2795 2437
Size: 358 3808
BstAPI GCAn_nnn'nTGC
Cuts at: 1543 1543
Size: 4166
BstBI TT'CG_AA
Cuts at: 2527 2527
Size: 4166
BstYI r'GATC_y
Cuts at: 1 327 549 706 1191 1658 1681 2016 2262
Size: 326 222 157 485 467 23 335 246
Cuts at: 2262 3274 3360 3372 3718 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 485 467 449 346 335 326 246
222 157 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4166
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 642 2116 2297 2460 642
Size: 1474 181 163 2348
Fragments arranged by size:
2348 1474 181 163
BtsI GCAGTG_nn'
Cuts at: 1409 1409
Size: 4166
DraI TTT'AAA
Cuts at: 1165 1165
Size: 4166
DrdI GACnn_nn'nnGTC
Cuts at: 1872 2730 1872
Size: 858 3308
EaeI y'GGCC_r
Cuts at: 134 1751 1925 2316 2343 3688 134
Size: 1617 174 391 27 1345 612
Fragments arranged by size:
1617 1345 612 391 174 27
EagI C'GGCC_G
Cuts at: 134 1751 134
Size: 1617 2549
EarI CTCTTCn'nnn_
Cuts at: 194 302 730 2189 2399 194
Size: 108 428 1459 210 1961
Fragments arranged by size:
1961 1459 428 210 108
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2374 2684 2830 3976 2374
Size: 310 146 1146 2564
Fragments arranged by size:
2564 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1178 1178
Size: 4166
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 149 830 1991 2423 3170 3438 149
Size: 681 1161 432 747 268 877
Fragments arranged by size:
1161 877 747 681 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1305 1305
Size: 4166
EcoRI G'AATT_C
Cuts at: 1170 1170
Size: 4166
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2185 2185
Size: 4166
FspI TGC'GCA
Cuts at: 1947 1947
Size: 4166
HaeII r_GCGC'y
Cuts at: 438 1600 1848 2870 3849 438
Size: 1162 248 1022 979 755
Fragments arranged by size:
1162 1022 979 755 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 142 145 174 177 631 663 2008 2038 2040
Size: 3 29 3 454 32 1345 30 2
Cuts at: 2040 2070 142
Size: 30 2238
Fragments arranged by size:
2238 1345 454 32 30 30 29 3
3 2
HincII GTy'rAC
Cuts at: 315 1205 315
Size: 890 3276
HindIII A'AGCT_T
Cuts at: 346 346
Size: 4166
KasI G'GCGC_C
Cuts at: 1596 1844 1596
Size: 248 3918
KpnI G_GTAC'C
Cuts at: 1186 1186
Size: 4166
MfeI C'AATT_G
Cuts at: 581 581
Size: 4166
MluI A'CGCG_T
Cuts at: 3599 3599
Size: 4166
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2837 3021 2837
Size: 184 3982
MscI TGG'CCA
Cuts at: 1927 1927
Size: 4166
MslI CAynn'nnrTG
Cuts at: 934 949 2155 2282 934
Size: 15 1206 127 2818
Fragments arranged by size:
2818 1206 127 15
MspA1I CmG'CkG
Cuts at: 1277 1591 1951 2964 3209 1277
Size: 314 360 1013 245 2234
Fragments arranged by size:
2234 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2347 2347
Size: 4166
NarI GG'CG_CC
Cuts at: 1597 1845 1597
Size: 248 3918
NcoI C'CATG_G
Cuts at: 107 107
Size: 4166
NgoMIV G'CCGG_C
Cuts at: 2345 2345
Size: 4166
NspI r_CATG'y
Cuts at: 1219 1719 2626 1219
Size: 500 907 2759
Fragments arranged by size:
2759 907 500
PciI A'CATG_T
Cuts at: 2622 2622
Size: 4166
PmeI GTTT'AAAC
Cuts at: 1165 1165
Size: 4166
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3305 3337 3305
Size: 32 4134
PstI C_TGCA'G
Cuts at: 1213 1898 1213
Size: 685 3481
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4166
PvuII CAG'CTG
Cuts at: 1591 1951 1591
Size: 360 3806
RsrII CG'GwC_CG
Cuts at: 2361 2361
Size: 4166
SacI G_AGCT'C
Cuts at: 1180 1180
Size: 4166
SalI G'TCGA_C
Cuts at: 313 1203 313
Size: 890 3276
SapI GCTCTTCn'nnn_
Cuts at: 194 2189 2399 194
Size: 1995 210 1961
Fragments arranged by size:
1995 1961 210
SbfI CC_TGCA'GG
Cuts at: 1213 1213
Size: 4166
ScaI AGT'ACT
Cuts at: 367 3683 367
Size: 3316 850
SfcI C'TryA_G
Cuts at: 33 683 1209 1894 2887 3078 33
Size: 650 526 685 993 191 1121
Fragments arranged by size:
1121 993 685 650 526 191
SfoI GGC'GCC
Cuts at: 1598 1846 1598
Size: 248 3918
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4166
SmaI CCC'GGG
Cuts at: 1188 1188
Size: 4166
SmlI C'TyrA_G
Cuts at: 1321 2236 2728 2990 1321
Size: 915 492 262 2497
Fragments arranged by size:
2497 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4166
SphI G_CATG'C
Cuts at: 1219 1719 1219
Size: 500 3666
StyI C'CwwG_G
Cuts at: 107 1145 1300 1654 107
Size: 1038 155 354 2619
Fragments arranged by size:
2619 1038 354 155
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1749 1882 2341 2550 1749
Size: 133 459 209 3365
Fragments arranged by size:
3365 459 209 133
TatI w'GTAC_w
Cuts at: 365 518 959 3681 365
Size: 153 441 2722 850
Fragments arranged by size:
2722 850 441 153
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3683 3715 3683
Size: 32 4134
Tth111I GACn'n_nGTC
Cuts at: 1963 1963
Size: 4166
XbaI T'CTAG_A
Cuts at: 1197 1197
Size: 4166
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1661 1661
Size: 4166
XmaI C'CCGG_G
Cuts at: 1186 1186
Size: 4166
XmnI GAAnn'nnTTC
Cuts at: 219 367 794 219
Size: 148 427 3591
Fragments arranged by size:
3591 427 148
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PmeI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI BpmI Bpu10I BsaXI BseRI BsiWI BsmI BsmBI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HpaI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
ZraI