Restriction map for
pF1KB6459
(Circular) (Six-base) MAPSORT of: pF1KB6459.seq Check: 7656 from: 1 to: 4067
pF1KB6459 4067 bp
With 182 enzymes: SgfI *
October 7, 2009 14:20 ..
AccI GT'mk_AC
Cuts at: 1105 1105
Size: 4067
Acc65I G'GTAC_C
Cuts at: 1083 1083
Size: 4067
AclI AA'CG_TT
Cuts at: 3907 3907
Size: 4067
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1892 2324 3071 3339 1892
Size: 432 747 268 2620
Fragments arranged by size:
2620 747 432 268
AflIII A'CryG_T
Cuts at: 1419 2523 3500 1419
Size: 1104 977 1986
Fragments arranged by size:
1986 1104 977
AgeI A'CCGG_T
Cuts at: 2455 2455
Size: 4067
AhdI GACnn_n'nnGTC
Cuts at: 561 561
Size: 4067
AlwNI CAG_nnn'CTG
Cuts at: 577 1372 2939 577
Size: 795 1567 1705
Fragments arranged by size:
1705 1567 795
ApaLI G'TGCA_C
Cuts at: 2837 2837
Size: 4067
ApoI r'AATT_y
Cuts at: 458 491 610 876 1071 3420 3676 458
Size: 33 119 266 195 2349 256 849
Fragments arranged by size:
2349 849 266 256 195 119 33
AseI AT'TA_AT
Cuts at: 20 589 784 20
Size: 569 195 3303
Fragments arranged by size:
3303 569 195
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4067
AvaI C'yCGr_G
Cuts at: 1087 1087
Size: 4067
BamHI G'GATC_C
Cuts at: 1092 1092
Size: 4067
BanI G'GyrC_C
Cuts at: 1083 1497 1745 1780 1083
Size: 414 248 35 3370
Fragments arranged by size:
3370 414 248 35
BanII G_rGCy'C
Cuts at: 297 1081 2111 297
Size: 784 1030 2253
Fragments arranged by size:
2253 1030 784
BbeI G_GCGC'C
Cuts at: 1501 1749 1501
Size: 248 3819
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 919 953 919
Size: 34 4033
BciVI GTATCCnnnnn_n'
Cuts at: 1965 2726 4067 1965
Size: 761 1341 1965
Fragments arranged by size:
1965 1341 761
BclI T'GATC_A
Cuts at: 1587 1587
Size: 4067
BglII A'GATC_T
Cuts at: 1582 3619 1582
Size: 2037 2030
BlpI GC'TnA_GC
Cuts at: 1179 1179
Size: 4067
Bme1580I G_kGCm'C
Cuts at: 1692 1785 2841 1692
Size: 93 1056 2918
Fragments arranged by size:
2918 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 587 1279 1360 1695 3839 587
Size: 692 81 335 2144 815
Fragments arranged by size:
2144 815 692 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2434 2434
Size: 4067
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 187 964 1243 2122 2614 2912 187
Size: 777 279 879 492 298 1342
Fragments arranged by size:
1342 879 777 492 298 279
BsaI GGTCTCn'nnnn_
Cuts at: 3771 3771
Size: 4067
BsaAI yAC'GTr
Cuts at: 1420 1420
Size: 4067
BsaBI GATnn'nnATC
Cuts at: 6 1016 1581 3618 6
Size: 1010 565 2037 455
Fragments arranged by size:
2037 1010 565 455
BsaHI Gr'CG_yC
Cuts at: 1498 1746 2448 3944 1498
Size: 248 702 1496 1621
Fragments arranged by size:
1621 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1256 1777 2455 2729 2876 3287 1256
Size: 521 678 274 147 411 2036
Fragments arranged by size:
2036 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 358 358
Size: 4067
BseYI C'CCAG_C
Cuts at: 696 1492 2250 2827 3323 696
Size: 796 758 577 496 1440
Fragments arranged by size:
1440 796 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 590 590
Size: 4067
BsiEI CG_ry'CG
Cuts at: 104 1609 1655 2863 104
Size: 1505 46 1208 1308
Fragments arranged by size:
1505 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 185 297 709 1081 1859 2049 2841 185
Size: 112 412 372 778 190 792 1411
Fragments arranged by size:
1411 792 778 412 372 190 112
Bsp1286I G_dGCh'C
Cuts at: 185 297 709 1081 1692 1785 1859 2049 2111
Size: 112 412 372 611 93 74 190 62
Cuts at: 2111 2841 185
Size: 730 1411
Fragments arranged by size:
1411 730 611 412 372 190 112 93
74 62
BspEI T'CCGG_A
Cuts at: 3287 3287
Size: 4067
BspHI T'CATG_A
Cuts at: 323 606 3243 323
Size: 283 2637 1147
Fragments arranged by size:
2637 1147 283
BspMI ACCTGCnnnn'nnnn_
Cuts at: 276 1117 1331 1633 2014 276
Size: 841 214 302 381 2329
Fragments arranged by size:
2329 841 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2359 2413 3897 4062 50
Size: 2309 54 1484 165 55
Fragments arranged by size:
2309 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1979 1979
Size: 4067
BsrFI r'CCGG_y
Cuts at: 2065 2246 2455 2065
Size: 181 209 3677
Fragments arranged by size:
3677 209 181
BssSI C'ACGA_G
Cuts at: 338 2338 2696 338
Size: 2000 358 1709
Fragments arranged by size:
2000 1709 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1444 1444
Size: 4067
BstBI TT'CG_AA
Cuts at: 2428 2428
Size: 4067
BstYI r'GATC_y
Cuts at: 1 1011 1092 1559 1582 1917 2163 3175 3261
Size: 1010 81 467 23 335 246 1012 86
Cuts at: 3261 3273 3619 1
Size: 12 346 449
Fragments arranged by size:
1012 1010 467 449 346 335 246 86
81 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4067
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2017 2198 2361 2017
Size: 181 163 3723
Fragments arranged by size:
3723 181 163
BtsI GCAGTG_nn'
Cuts at: 1310 1310
Size: 4067
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 547 582 547
Size: 35 4032
DraI TTT'AAA
Cuts at: 962 1066 962
Size: 104 3963
DrdI GACnn_nn'nnGTC
Cuts at: 1773 2631 1773
Size: 858 3209
EaeI y'GGCC_r
Cuts at: 595 1652 1826 2217 2244 3589 595
Size: 1057 174 391 27 1345 1073
Fragments arranged by size:
1345 1073 1057 391 174 27
EagI C'GGCC_G
Cuts at: 1652 1652
Size: 4067
EarI CTCTTCn'nnn_
Cuts at: 2090 2300 2090
Size: 210 3857
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2275 2585 2731 3877 2275
Size: 310 146 1146 2465
Fragments arranged by size:
2465 1146 310 146
EcoICRI GAG'CTC
Cuts at: 295 1079 295
Size: 784 3283
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1892 2324 3071 3339 1892
Size: 432 747 268 2620
Fragments arranged by size:
2620 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1206 1206
Size: 4067
EcoRI G'AATT_C
Cuts at: 1071 1071
Size: 4067
EcoRV GAT'ATC
Cuts at: 1039 1039
Size: 4067
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2086 2086
Size: 4067
FspI TGC'GCA
Cuts at: 1848 1848
Size: 4067
HaeII r_GCGC'y
Cuts at: 1501 1749 2771 3750 1501
Size: 248 1022 979 1818
Fragments arranged by size:
1818 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 321 353 547 579 1909 1939 1941 1971 321
Size: 32 194 32 1330 30 2 30 2417
Fragments arranged by size:
2417 1330 194 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1106 1106
Size: 4067
HindIII A'AGCT_T
Cuts at: 286 520 286
Size: 234 3833
KasI G'GCGC_C
Cuts at: 1497 1745 1497
Size: 248 3819
KpnI G_GTAC'C
Cuts at: 1087 1087
Size: 4067
MluI A'CGCG_T
Cuts at: 3500 3500
Size: 4067
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 719 2738 2922 719
Size: 2019 184 1864
Fragments arranged by size:
2019 1864 184
MscI TGG'CCA
Cuts at: 1828 1828
Size: 4067
MslI CAynn'nnrTG
Cuts at: 328 853 2056 2183 328
Size: 525 1203 127 2212
Fragments arranged by size:
2212 1203 525 127
MspA1I CmG'CkG
Cuts at: 1178 1492 1852 2865 3110 1178
Size: 314 360 1013 245 2135
Fragments arranged by size:
2135 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2248 2248
Size: 4067
NarI GG'CG_CC
Cuts at: 1498 1746 1498
Size: 248 3819
NcoI C'CATG_G
Cuts at: 107 107
Size: 4067
NgoMIV G'CCGG_C
Cuts at: 2246 2246
Size: 4067
NspI r_CATG'y
Cuts at: 1120 1620 2527 1120
Size: 500 907 2660
Fragments arranged by size:
2660 907 500
PasI CC'CwG_GG
Cuts at: 506 506
Size: 4067
PciI A'CATG_T
Cuts at: 2523 2523
Size: 4067
PflMI CCAn_nnn'nTGG
Cuts at: 898 898
Size: 4067
PmeI GTTT'AAAC
Cuts at: 1066 1066
Size: 4067
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3206 3238 3206
Size: 32 4035
PsiI TTA'TAA
Cuts at: 722 722
Size: 4067
PstI C_TGCA'G
Cuts at: 273 1114 1799 273
Size: 841 685 2541
Fragments arranged by size:
2541 841 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4067
PvuII CAG'CTG
Cuts at: 1492 1852 1492
Size: 360 3707
RsrII CG'GwC_CG
Cuts at: 2262 2262
Size: 4067
SacI G_AGCT'C
Cuts at: 297 1081 297
Size: 784 3283
SalI G'TCGA_C
Cuts at: 1104 1104
Size: 4067
SapI GCTCTTCn'nnn_
Cuts at: 2090 2300 2090
Size: 210 3857
SbfI CC_TGCA'GG
Cuts at: 1114 1114
Size: 4067
ScaI AGT'ACT
Cuts at: 3584 3584
Size: 4067
SfcI C'TryA_G
Cuts at: 33 269 1110 1795 2788 2979 33
Size: 236 841 685 993 191 1121
Fragments arranged by size:
1121 993 841 685 236 191
SfoI GGC'GCC
Cuts at: 1499 1747 1499
Size: 248 3819
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4067
SmaI CCC'GGG
Cuts at: 1089 1089
Size: 4067
SmlI C'TyrA_G
Cuts at: 202 979 1222 2137 2629 2891 202
Size: 777 243 915 492 262 1378
Fragments arranged by size:
1378 915 777 492 262 243
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4067
SphI G_CATG'C
Cuts at: 1120 1620 1120
Size: 500 3567
SspI AAT'ATT
Cuts at: 1027 1027
Size: 4067
StuI AGG'CCT
Cuts at: 915 915
Size: 4067
StyI C'CwwG_G
Cuts at: 107 772 1201 1555 107
Size: 665 429 354 2619
Fragments arranged by size:
2619 665 429 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1650 1783 2242 2451 1650
Size: 133 459 209 3266
Fragments arranged by size:
3266 459 209 133
TatI w'GTAC_w
Cuts at: 479 762 3582 479
Size: 283 2820 964
Fragments arranged by size:
2820 964 283
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3584 3616 3584
Size: 32 4035
Tth111I GACn'n_nGTC
Cuts at: 1864 1864
Size: 4067
XbaI T'CTAG_A
Cuts at: 1098 1098
Size: 4067
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 241 1562 241
Size: 1321 2746
XmaI C'CCGG_G
Cuts at: 1087 1087
Size: 4067
XmnI GAAnn'nnTTC
Cuts at: 822 822
Size: 4067
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BcgI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PflMI PmeI PpiI PsiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BfrBI BglI BmgBI
BmtI BpmI Bpu10I BsaXI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI DraIII EcoNI
FseI FspAI HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PfoI PmlI PpuMI PshAI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SwaI XhoI ZraI