Restriction map for
pF1KB6461
(Circular) (Six-base) MAPSORT of: pF1KB6461.seq Check: 6229 from: 1 to: 4076
pF1KB6461 4076 bp
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 555 555
Size: 4076
AccI GT'mk_AC
Cuts at: 1114 1114
Size: 4076
Acc65I G'GTAC_C
Cuts at: 1092 1092
Size: 4076
AclI AA'CG_TT
Cuts at: 3916 3916
Size: 4076
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 494 811 1901 2333 3080 3348 494
Size: 317 1090 432 747 268 1222
Fragments arranged by size:
1222 1090 747 432 317 268
AflIII A'CryG_T
Cuts at: 590 1428 2532 3509 590
Size: 838 1104 977 1157
Fragments arranged by size:
1157 1104 977 838
AgeI A'CCGG_T
Cuts at: 2464 2464
Size: 4076
AleI CACnn'nnGTG
Cuts at: 332 332
Size: 4076
AlwNI CAG_nnn'CTG
Cuts at: 381 839 1030 1381 2948 381
Size: 458 191 351 1567 1509
Fragments arranged by size:
1567 1509 458 351 191
ApaLI G'TGCA_C
Cuts at: 2846 2846
Size: 4076
ApoI r'AATT_y
Cuts at: 1080 3429 3685 1080
Size: 2349 256 1471
Fragments arranged by size:
2349 1471 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4076
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4076
AvaI C'yCGr_G
Cuts at: 1096 1096
Size: 4076
BamHI G'GATC_C
Cuts at: 1101 1101
Size: 4076
BanI G'GyrC_C
Cuts at: 646 940 1092 1506 1754 1789 646
Size: 294 152 414 248 35 2933
Fragments arranged by size:
2933 414 294 248 152 35
BanII G_rGCy'C
Cuts at: 846 857 901 1090 2120 846
Size: 11 44 189 1030 2802
Fragments arranged by size:
2802 1030 189 44 11
BbeI G_GCGC'C
Cuts at: 944 1510 1758 944
Size: 566 248 3262
Fragments arranged by size:
3262 566 248
BciVI GTATCCnnnnn_n'
Cuts at: 1974 2735 4076 1974
Size: 761 1341 1974
Fragments arranged by size:
1974 1341 761
BclI T'GATC_A
Cuts at: 1596 1596
Size: 4076
BglI GCCn_nnn'nGGC
Cuts at: 758 758
Size: 4076
BglII A'GATC_T
Cuts at: 462 1591 3628 462
Size: 1129 2037 910
Fragments arranged by size:
2037 1129 910
BlpI GC'TnA_GC
Cuts at: 1188 1188
Size: 4076
Bme1580I G_kGCm'C
Cuts at: 598 649 1701 1794 2850 598
Size: 51 1052 93 1056 1824
Fragments arranged by size:
1824 1056 1052 93 51
BmrI ACTGGGnnnn_n'
Cuts at: 255 351 849 1288 1369 1704 3848 255
Size: 96 498 439 81 335 2144 483
Fragments arranged by size:
2144 498 483 439 335 96 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2443 2443
Size: 4076
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 403 830 403
Size: 427 3649
Bpu10I CC'TnA_GC
Cuts at: 550 550
Size: 4076
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 287 1252 2131 2623 2921 287
Size: 965 879 492 298 1442
Fragments arranged by size:
1442 965 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3780 3780
Size: 4076
BsaAI yAC'GTr
Cuts at: 593 825 1429 593
Size: 232 604 3240
Fragments arranged by size:
3240 604 232
BsaBI GATnn'nnATC
Cuts at: 6 414 1590 3627 6
Size: 408 1176 2037 455
Fragments arranged by size:
2037 1176 455 408
BsaHI Gr'CG_yC
Cuts at: 917 941 1507 1755 2457 3953 917
Size: 24 566 248 702 1496 1040
Fragments arranged by size:
1496 1040 702 566 248 24
BsaWI w'CCGG_w
Cuts at: 901 1265 1786 2464 2738 2885 3296 901
Size: 364 521 678 274 147 411 1681
Fragments arranged by size:
1681 678 521 411 364 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 141 171 745 775 141
Size: 30 574 30 3442
Fragments arranged by size:
3442 574 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 151 550 736 151
Size: 399 186 3491
Fragments arranged by size:
3491 399 186
BseYI C'CCAG_C
Cuts at: 186 214 936 1501 2259 2836 3332 186
Size: 28 722 565 758 577 496 930
Fragments arranged by size:
930 758 722 577 565 496 28
BsiEI CG_ry'CG
Cuts at: 104 906 930 1038 1618 1664 2872 104
Size: 802 24 108 580 46 1208 1308
Fragments arranged by size:
1308 1208 802 580 108 46 24
BsiHKAI G_wGCw'C
Cuts at: 1090 1868 2058 2850 1090
Size: 778 190 792 2316
Fragments arranged by size:
2316 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 303 303
Size: 4076
Bsp1286I G_dGCh'C
Cuts at: 598 649 846 857 901 1090 1701 1794 1868
Size: 51 197 11 44 189 611 93 74
Cuts at: 1868 2058 2120 2850 598
Size: 190 62 730 1824
Fragments arranged by size:
1824 730 611 197 190 189 93 74
62 51 44 11
BspEI T'CCGG_A
Cuts at: 3296 3296
Size: 4076
BspHI T'CATG_A
Cuts at: 3252 3252
Size: 4076
BspMI ACCTGCnnnn'nnnn_
Cuts at: 555 1126 1340 1642 2023 555
Size: 571 214 302 381 2608
Fragments arranged by size:
2608 571 381 302 214
BsrBI CCG'CTC
Cuts at: 50 205 2368 2422 3906 4071 50
Size: 155 2163 54 1484 165 55
Fragments arranged by size:
2163 1484 165 155 55 54
BsrDI GCAATG_nn'
Cuts at: 1988 1988
Size: 4076
BsrFI r'CCGG_y
Cuts at: 757 2074 2255 2464 757
Size: 1317 181 209 2369
Fragments arranged by size:
2369 1317 209 181
BssSI C'ACGA_G
Cuts at: 2347 2705 2347
Size: 358 3718
BstAPI GCAn_nnn'nTGC
Cuts at: 593 1453 593
Size: 860 3216
BstBI TT'CG_AA
Cuts at: 2437 2437
Size: 4076
BstEII G'GTnAC_C
Cuts at: 195 334 195
Size: 139 3937
BstXI CCAn_nnnn'nTGG
Cuts at: 318 318
Size: 4076
BstYI r'GATC_y
Cuts at: 1 462 1101 1568 1591 1926 2172 3184 3270
Size: 461 639 467 23 335 246 1012 86
Cuts at: 3270 3282 3628 1
Size: 12 346 449
Fragments arranged by size:
1012 639 467 461 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 330 995 1004 107
Size: 223 665 9 3179
Fragments arranged by size:
3179 665 223 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2026 2207 2370 2026
Size: 181 163 3732
Fragments arranged by size:
3732 181 163
BtsI GCAGTG_nn'
Cuts at: 661 1319 661
Size: 658 3418
DraI TTT'AAA
Cuts at: 1075 1075
Size: 4076
DrdI GACnn_nn'nnGTC
Cuts at: 1782 2640 1782
Size: 858 3218
EaeI y'GGCC_r
Cuts at: 962 1035 1661 1835 2226 2253 3598 962
Size: 73 626 174 391 27 1345 1440
Fragments arranged by size:
1440 1345 626 391 174 73 27
EagI C'GGCC_G
Cuts at: 1035 1661 1035
Size: 626 3450
EarI CTCTTCn'nnn_
Cuts at: 272 2099 2309 272
Size: 1827 210 2039
Fragments arranged by size:
2039 1827 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2284 2594 2740 3886 2284
Size: 310 146 1146 2474
Fragments arranged by size:
2474 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1088 1088
Size: 4076
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 403 494 811 830 1901 2333 3080 3348 403
Size: 91 317 19 1071 432 747 268 1131
Fragments arranged by size:
1131 1071 747 432 317 268 91 19
EcoO109I rG'GnC_Cy
Cuts at: 1009 1215 1009
Size: 206 3870
EcoRI G'AATT_C
Cuts at: 1080 1080
Size: 4076
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2095 2095
Size: 4076
FspI TGC'GCA
Cuts at: 1857 1857
Size: 4076
HaeII r_GCGC'y
Cuts at: 944 1510 1758 2780 3759 944
Size: 566 248 1022 979 1261
Fragments arranged by size:
1261 1022 979 566 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 189 221 1918 1948 1950 1980 189
Size: 32 1697 30 2 30 2285
Fragments arranged by size:
2285 1697 32 30 30 2
HincII GTy'rAC
Cuts at: 1115 1115
Size: 4076
HindIII A'AGCT_T
Cuts at: 506 506
Size: 4076
KasI G'GCGC_C
Cuts at: 940 1506 1754 940
Size: 566 248 3262
Fragments arranged by size:
3262 566 248
KpnI G_GTAC'C
Cuts at: 1096 1096
Size: 4076
MluI A'CGCG_T
Cuts at: 3509 3509
Size: 4076
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 642 2747 2931 642
Size: 2105 184 1787
Fragments arranged by size:
2105 1787 184
MscI TGG'CCA
Cuts at: 964 1837 964
Size: 873 3203
MslI CAynn'nnrTG
Cuts at: 332 2065 2192 332
Size: 1733 127 2216
Fragments arranged by size:
2216 1733 127
MspA1I CmG'CkG
Cuts at: 273 575 699 960 997 1187 1501 1861 2874
Size: 302 124 261 37 190 314 360 1013
Cuts at: 2874 3119 273
Size: 245 1230
Fragments arranged by size:
1230 1013 360 314 302 261 245 190
124 37
NaeI GCC'GGC
Cuts at: 2257 2257
Size: 4076
NarI GG'CG_CC
Cuts at: 941 1507 1755 941
Size: 566 248 3262
Fragments arranged by size:
3262 566 248
NcoI C'CATG_G
Cuts at: 107 1004 107
Size: 897 3179
NgoMIV G'CCGG_C
Cuts at: 2255 2255
Size: 4076
NspI r_CATG'y
Cuts at: 748 971 1129 1629 2536 748
Size: 223 158 500 907 2288
Fragments arranged by size:
2288 907 500 223 158
PciI A'CATG_T
Cuts at: 2532 2532
Size: 4076
PflMI CCAn_nnn'nTGG
Cuts at: 656 656
Size: 4076
PfoI T'CCnGG_A
Cuts at: 436 530 763 436
Size: 94 233 3749
Fragments arranged by size:
3749 233 94
PmeI GTTT'AAAC
Cuts at: 1075 1075
Size: 4076
PmlI CAC'GTG
Cuts at: 593 825 593
Size: 232 3844
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3215 3247 3215
Size: 32 4044
PpuMI rG'GwC_Cy
Cuts at: 1009 1009
Size: 4076
PstI C_TGCA'G
Cuts at: 1123 1808 1123
Size: 685 3391
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4076
PvuII CAG'CTG
Cuts at: 273 699 960 1501 1861 273
Size: 426 261 541 360 2488
Fragments arranged by size:
2488 541 426 360 261
RsrII CG'GwC_CG
Cuts at: 2271 2271
Size: 4076
SacI G_AGCT'C
Cuts at: 1090 1090
Size: 4076
SacII CC_GC'GG
Cuts at: 998 998
Size: 4076
SalI G'TCGA_C
Cuts at: 1113 1113
Size: 4076
SapI GCTCTTCn'nnn_
Cuts at: 2099 2309 2099
Size: 210 3866
SbfI CC_TGCA'GG
Cuts at: 1123 1123
Size: 4076
ScaI AGT'ACT
Cuts at: 3593 3593
Size: 4076
SfcI C'TryA_G
Cuts at: 33 831 1119 1804 2797 2988 33
Size: 798 288 685 993 191 1121
Fragments arranged by size:
1121 993 798 685 288 191
SfoI GGC'GCC
Cuts at: 942 1508 1756 942
Size: 566 248 3262
Fragments arranged by size:
3262 566 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4076
SmaI CCC'GGG
Cuts at: 1098 1098
Size: 4076
SmlI C'TyrA_G
Cuts at: 302 1231 2146 2638 2900 302
Size: 929 915 492 262 1478
Fragments arranged by size:
1478 929 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4076
SphI G_CATG'C
Cuts at: 748 1129 1629 748
Size: 381 500 3195
Fragments arranged by size:
3195 500 381
SspI AAT'ATT
Cuts at: 124 124
Size: 4076
StyI C'CwwG_G
Cuts at: 107 1004 1210 1564 107
Size: 897 206 354 2619
Fragments arranged by size:
2619 897 354 206
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 798 936 1659 1792 2251 2460 798
Size: 138 723 133 459 209 2414
Fragments arranged by size:
2414 723 459 209 138 133
TatI w'GTAC_w
Cuts at: 3591 3591
Size: 4076
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 745 777 3593 3625 745
Size: 32 2816 32 1196
Fragments arranged by size:
2816 1196 32 32
Tth111I GACn'n_nGTC
Cuts at: 1873 1873
Size: 4076
XbaI T'CTAG_A
Cuts at: 1107 1107
Size: 4076
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1571 1571
Size: 4076
XmaI C'CCGG_G
Cuts at: 1096 1096
Size: 4076
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BmgBI BmtI
BsgI BsiWI BsmI BsrGI BssHII BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PshAI
PsiI PspOMI PspXI PsrI SanDI SexAI SfiI SgrAI
SnaBI SrfI StuI SwaI XhoI XmnI ZraI