Restriction map for
pF1KB6463
(Circular) (Six-base) MAPSORT of: pF1KB6463.seq Check: 1070 from: 1 to: 4079
pF1KB6463 4079 bp
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AccI GT'mk_AC
Cuts at: 1117 1117
Size: 4079
Acc65I G'GTAC_C
Cuts at: 520 1095 520
Size: 575 3504
AclI AA'CG_TT
Cuts at: 3919 3919
Size: 4079
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 688 1015 1904 2336 3083 3351 688
Size: 327 889 432 747 268 1416
Fragments arranged by size:
1416 889 747 432 327 268
AflIII A'CryG_T
Cuts at: 1431 2535 3512 1431
Size: 1104 977 1998
Fragments arranged by size:
1998 1104 977
AgeI A'CCGG_T
Cuts at: 2467 2467
Size: 4079
AleI CACnn'nnGTG
Cuts at: 956 956
Size: 4079
AlwNI CAG_nnn'CTG
Cuts at: 1384 2951 1384
Size: 1567 2512
ApaLI G'TGCA_C
Cuts at: 2849 2849
Size: 4079
ApoI r'AATT_y
Cuts at: 286 501 539 868 1083 3432 3688 286
Size: 215 38 329 215 2349 256 677
Fragments arranged by size:
2349 677 329 256 215 215 38
AseI AT'TA_AT
Cuts at: 20 662 20
Size: 642 3437
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4079
AvaI C'yCGr_G
Cuts at: 1099 1099
Size: 4079
BamHI G'GATC_C
Cuts at: 162 1104 162
Size: 942 3137
BanI G'GyrC_C
Cuts at: 520 1095 1509 1757 1792 520
Size: 575 414 248 35 2807
Fragments arranged by size:
2807 575 414 248 35
BanII G_rGCy'C
Cuts at: 1093 2123 1093
Size: 1030 3049
BbeI G_GCGC'C
Cuts at: 1513 1761 1513
Size: 248 3831
BbsI GAAGACnn'nnnn_
Cuts at: 224 452 224
Size: 228 3851
BciVI GTATCCnnnnn_n'
Cuts at: 1977 2738 4079 1977
Size: 761 1341 1977
Fragments arranged by size:
1977 1341 761
BclI T'GATC_A
Cuts at: 1599 1599
Size: 4079
BglII A'GATC_T
Cuts at: 387 1594 3631 387
Size: 1207 2037 835
Fragments arranged by size:
2037 1207 835
BlpI GC'TnA_GC
Cuts at: 1191 1191
Size: 4079
Bme1580I G_kGCm'C
Cuts at: 726 1704 1797 2853 726
Size: 978 93 1056 1952
Fragments arranged by size:
1952 1056 978 93
BmrI ACTGGGnnnn_n'
Cuts at: 163 519 1291 1372 1707 3851 163
Size: 356 772 81 335 2144 391
Fragments arranged by size:
2144 772 391 356 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2446 2446
Size: 4079
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1255 2134 2626 2924 1255
Size: 879 492 298 2410
Fragments arranged by size:
2410 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3783 3783
Size: 4079
BsaAI yAC'GTr
Cuts at: 1432 1432
Size: 4079
BsaBI GATnn'nnATC
Cuts at: 6 1593 3630 6
Size: 1587 2037 455
Fragments arranged by size:
2037 1587 455
BsaHI Gr'CG_yC
Cuts at: 112 1510 1758 2460 3956 112
Size: 1398 248 702 1496 235
Fragments arranged by size:
1496 1398 702 248 235
BsaWI w'CCGG_w
Cuts at: 1268 1789 2467 2741 2888 3299 1268
Size: 521 678 274 147 411 2048
Fragments arranged by size:
2048 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 895 895
Size: 4079
BseYI C'CCAG_C
Cuts at: 1504 2262 2839 3335 1504
Size: 758 577 496 2248
Fragments arranged by size:
2248 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1621 1667 2875 104
Size: 1517 46 1208 1308
Fragments arranged by size:
1517 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1093 1871 2061 2853 1093
Size: 778 190 792 2319
Fragments arranged by size:
2319 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 726 1093 1704 1797 1871 2061 2123 2853 726
Size: 367 611 93 74 190 62 730 1952
Fragments arranged by size:
1952 730 611 367 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3299 3299
Size: 4079
BspHI T'CATG_A
Cuts at: 1049 3255 1049
Size: 2206 1873
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1129 1343 1645 2026 1129
Size: 214 302 381 3182
Fragments arranged by size:
3182 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2371 2425 3909 4074 50
Size: 2321 54 1484 165 55
Fragments arranged by size:
2321 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 459 1991 459
Size: 1532 2547
BsrFI r'CCGG_y
Cuts at: 2077 2258 2467 2077
Size: 181 209 3689
Fragments arranged by size:
3689 209 181
BssSI C'ACGA_G
Cuts at: 2350 2708 2350
Size: 358 3721
BstAPI GCAn_nnn'nTGC
Cuts at: 1456 1456
Size: 4079
BstBI TT'CG_AA
Cuts at: 2440 2440
Size: 4079
BstXI CCAn_nnnn'nTGG
Cuts at: 633 633
Size: 4079
BstYI r'GATC_y
Cuts at: 1 162 387 1104 1571 1594 1929 2175 3187
Size: 161 225 717 467 23 335 246 1012
Cuts at: 3187 3273 3285 3631 1
Size: 86 12 346 449
Fragments arranged by size:
1012 717 467 449 346 335 246 225
161 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 896 896
Size: 4079
BtgI C'CryG_G
Cuts at: 107 134 107
Size: 27 4052
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2029 2210 2373 2029
Size: 181 163 3735
Fragments arranged by size:
3735 181 163
BtsI GCAGTG_nn'
Cuts at: 371 1322 371
Size: 951 3128
DraI TTT'AAA
Cuts at: 500 614 1078 500
Size: 114 464 3501
Fragments arranged by size:
3501 464 114
DrdI GACnn_nn'nnGTC
Cuts at: 1785 2643 1785
Size: 858 3221
EaeI y'GGCC_r
Cuts at: 1664 1838 2229 2256 3601 1664
Size: 174 391 27 1345 2142
Fragments arranged by size:
2142 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1664 1664
Size: 4079
EarI CTCTTCn'nnn_
Cuts at: 182 245 918 2102 2312 182
Size: 63 673 1184 210 1949
Fragments arranged by size:
1949 1184 673 210 63
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2287 2597 2743 3889 2287
Size: 310 146 1146 2477
Fragments arranged by size:
2477 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1091 1091
Size: 4079
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 688 1015 1904 2336 3083 3351 688
Size: 327 889 432 747 268 1416
Fragments arranged by size:
1416 889 747 432 327 268
EcoO109I rG'GnC_Cy
Cuts at: 1218 1218
Size: 4079
EcoRI G'AATT_C
Cuts at: 1083 1083
Size: 4079
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 774 2098 774
Size: 1324 2755
FspI TGC'GCA
Cuts at: 1860 1860
Size: 4079
HaeII r_GCGC'y
Cuts at: 1513 1761 2783 3762 1513
Size: 248 1022 979 1830
Fragments arranged by size:
1830 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1921 1951 1953 1983 1921
Size: 30 2 30 4017
Fragments arranged by size:
4017 30 30 2
HincII GTy'rAC
Cuts at: 1118 1118
Size: 4079
HindIII A'AGCT_T
Cuts at: 293 293
Size: 4079
KasI G'GCGC_C
Cuts at: 1509 1757 1509
Size: 248 3831
KpnI G_GTAC'C
Cuts at: 524 1099 524
Size: 575 3504
MluI A'CGCG_T
Cuts at: 3512 3512
Size: 4079
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1038 2750 2934 1038
Size: 1712 184 2183
Fragments arranged by size:
2183 1712 184
MscI TGG'CCA
Cuts at: 1840 1840
Size: 4079
MslI CAynn'nnrTG
Cuts at: 956 2068 2195 956
Size: 1112 127 2840
Fragments arranged by size:
2840 1112 127
MspA1I CmG'CkG
Cuts at: 600 1190 1504 1864 2877 3122 600
Size: 590 314 360 1013 245 1557
Fragments arranged by size:
1557 1013 590 360 314 245
NaeI GCC'GGC
Cuts at: 2260 2260
Size: 4079
NarI GG'CG_CC
Cuts at: 1510 1758 1510
Size: 248 3831
NcoI C'CATG_G
Cuts at: 107 107
Size: 4079
NgoMIV G'CCGG_C
Cuts at: 2258 2258
Size: 4079
NspI r_CATG'y
Cuts at: 1132 1632 2539 1132
Size: 500 907 2672
Fragments arranged by size:
2672 907 500
PciI A'CATG_T
Cuts at: 2535 2535
Size: 4079
PmeI GTTT'AAAC
Cuts at: 1078 1078
Size: 4079
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3218 3250 3218
Size: 32 4047
PstI C_TGCA'G
Cuts at: 344 378 1126 1811 344
Size: 34 748 685 2612
Fragments arranged by size:
2612 748 685 34
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4079
PvuII CAG'CTG
Cuts at: 600 1504 1864 600
Size: 904 360 2815
Fragments arranged by size:
2815 904 360
RsrII CG'GwC_CG
Cuts at: 2274 2274
Size: 4079
SacI G_AGCT'C
Cuts at: 1093 1093
Size: 4079
SalI G'TCGA_C
Cuts at: 1116 1116
Size: 4079
SapI GCTCTTCn'nnn_
Cuts at: 2102 2312 2102
Size: 210 3869
SbfI CC_TGCA'GG
Cuts at: 1126 1126
Size: 4079
ScaI AGT'ACT
Cuts at: 3596 3596
Size: 4079
SfcI C'TryA_G
Cuts at: 33 340 374 1122 1807 2800 2991 33
Size: 307 34 748 685 993 191 1121
Fragments arranged by size:
1121 993 748 685 307 191 34
SfoI GGC'GCC
Cuts at: 1511 1759 1511
Size: 248 3831
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4079
SmaI CCC'GGG
Cuts at: 1101 1101
Size: 4079
SmlI C'TyrA_G
Cuts at: 1234 2149 2641 2903 1234
Size: 915 492 262 2410
Fragments arranged by size:
2410 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4079
SphI G_CATG'C
Cuts at: 1132 1632 1132
Size: 500 3579
SspI AAT'ATT
Cuts at: 282 282
Size: 4079
StyI C'CwwG_G
Cuts at: 107 1213 1567 107
Size: 1106 354 2619
Fragments arranged by size:
2619 1106 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1662 1795 2254 2463 1662
Size: 133 459 209 3278
Fragments arranged by size:
3278 459 209 133
TatI w'GTAC_w
Cuts at: 3594 3594
Size: 4079
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3596 3628 3596
Size: 32 4047
Tth111I GACn'n_nGTC
Cuts at: 259 1876 259
Size: 1617 2462
XbaI T'CTAG_A
Cuts at: 1110 1110
Size: 4079
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 630 1574 630
Size: 944 3135
XmaI C'CCGG_G
Cuts at: 1099 1099
Size: 4079
XmnI GAAnn'nnTTC
Cuts at: 539 938 539
Size: 399 3680
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PmeI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI BpmI Bpu10I BsaXI BsgI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstZ17I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI StuI SwaI XhoI ZraI