Restriction map for
pF1KB6466
(Circular) (Six-base) MAPSORT of: pF1KB6466.seq Check: 5447 from: 1 to: 4169
pF1KB6466 4169 bp
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AccI GT'mk_AC
Cuts at: 1207 1207
Size: 4169
Acc65I G'GTAC_C
Cuts at: 480 1185 480
Size: 705 3464
AclI AA'CG_TT
Cuts at: 4009 4009
Size: 4169
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 301 524 728 1177 1994 2426 3173 3441 301
Size: 223 204 449 817 432 747 268 1029
Fragments arranged by size:
1029 817 747 449 432 268 223 204
AflIII A'CryG_T
Cuts at: 920 1521 2625 3602 920
Size: 601 1104 977 1487
Fragments arranged by size:
1487 1104 977 601
AgeI A'CCGG_T
Cuts at: 2557 2557
Size: 4169
AlwNI CAG_nnn'CTG
Cuts at: 939 1474 3041 939
Size: 535 1567 2067
Fragments arranged by size:
2067 1567 535
ApaI G_GGCC'C
Cuts at: 193 217 193
Size: 24 4145
ApaLI G'TGCA_C
Cuts at: 2939 2939
Size: 4169
ApoI r'AATT_y
Cuts at: 1173 3522 3778 1173
Size: 2349 256 1564
Fragments arranged by size:
2349 1564 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4169
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4169
AvaI C'yCGr_G
Cuts at: 186 216 818 1076 1189 186
Size: 30 602 258 113 3166
Fragments arranged by size:
3166 602 258 113 30
AvrII C'CTAG_G
Cuts at: 984 984
Size: 4169
BamHI G'GATC_C
Cuts at: 1194 1194
Size: 4169
BanI G'GyrC_C
Cuts at: 480 1185 1599 1847 1882 480
Size: 705 414 248 35 2767
Fragments arranged by size:
2767 705 414 248 35
BanII G_rGCy'C
Cuts at: 193 217 286 679 1103 1183 2213 193
Size: 24 69 393 424 80 1030 2149
Fragments arranged by size:
2149 1030 424 393 80 69 24
BbeI G_GCGC'C
Cuts at: 1603 1851 1603
Size: 248 3921
BbsI GAAGACnn'nnnn_
Cuts at: 996 996
Size: 4169
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 622 656 622
Size: 34 4135
BciVI GTATCCnnnnn_n'
Cuts at: 2067 2828 4169 2067
Size: 761 1341 2067
Fragments arranged by size:
2067 1341 761
BclI T'GATC_A
Cuts at: 995 1689 995
Size: 694 3475
BglII A'GATC_T
Cuts at: 266 1684 3721 266
Size: 1418 2037 714
Fragments arranged by size:
2037 1418 714
BlpI GC'TnA_GC
Cuts at: 1281 1281
Size: 4169
Bme1580I G_kGCm'C
Cuts at: 193 217 1794 1887 2943 193
Size: 24 1577 93 1056 1419
Fragments arranged by size:
1577 1419 1056 93 24
BmrI ACTGGGnnnn_n'
Cuts at: 1381 1462 1797 3941 1381
Size: 81 335 2144 1609
Fragments arranged by size:
2144 1609 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2536 2536
Size: 4169
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 663 663
Size: 4169
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 605 1345 2224 2716 3014 605
Size: 740 879 492 298 1760
Fragments arranged by size:
1760 879 740 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3873 3873
Size: 4169
BsaAI yAC'GTr
Cuts at: 1522 1522
Size: 4169
BsaBI GATnn'nnATC
Cuts at: 6 1683 3720 6
Size: 1677 2037 455
Fragments arranged by size:
2037 1677 455
BsaHI Gr'CG_yC
Cuts at: 461 869 1600 1848 2550 4046 461
Size: 408 731 248 702 1496 584
Fragments arranged by size:
1496 731 702 584 408 248
BsaWI w'CCGG_w
Cuts at: 1358 1879 2557 2831 2978 3389 1358
Size: 521 678 274 147 411 2138
Fragments arranged by size:
2138 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 874 904 1090 1120 874
Size: 30 186 30 3923
Fragments arranged by size:
3923 186 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1111 1111
Size: 4169
BseYI C'CCAG_C
Cuts at: 409 1090 1594 2352 2929 3425 409
Size: 681 504 758 577 496 1153
Fragments arranged by size:
1153 758 681 577 504 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1018 1018
Size: 4169
BsiEI CG_ry'CG
Cuts at: 104 1711 1757 2965 104
Size: 1607 46 1208 1308
Fragments arranged by size:
1607 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 679 1103 1183 1961 2151 2943 679
Size: 424 80 778 190 792 1905
Fragments arranged by size:
1905 792 778 424 190 80
BsmI GAATG_Cn'
Cuts at: 308 308
Size: 4169
Bsp1286I G_dGCh'C
Cuts at: 193 217 286 679 1103 1183 1794 1887 1961
Size: 24 69 393 424 80 611 93 74
Cuts at: 1961 2151 2213 2943 193
Size: 190 62 730 1419
Fragments arranged by size:
1419 730 611 424 393 190 93 80
74 69 62 24
BspEI T'CCGG_A
Cuts at: 3389 3389
Size: 4169
BspHI T'CATG_A
Cuts at: 3345 3345
Size: 4169
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1219 1433 1735 2116 1219
Size: 214 302 381 3272
Fragments arranged by size:
3272 381 302 214
BsrBI CCG'CTC
Cuts at: 50 207 2461 2515 3999 4164 50
Size: 157 2254 54 1484 165 55
Fragments arranged by size:
2254 1484 165 157 55 54
BsrDI GCAATG_nn'
Cuts at: 2081 2081
Size: 4169
BsrFI r'CCGG_y
Cuts at: 2167 2348 2557 2167
Size: 181 209 3779
Fragments arranged by size:
3779 209 181
BssSI C'ACGA_G
Cuts at: 2440 2798 2440
Size: 358 3811
BstAPI GCAn_nnn'nTGC
Cuts at: 1546 1546
Size: 4169
BstBI TT'CG_AA
Cuts at: 2530 2530
Size: 4169
BstYI r'GATC_y
Cuts at: 1 266 1194 1661 1684 2019 2265 3277 3363
Size: 265 928 467 23 335 246 1012 86
Cuts at: 3363 3375 3721 1
Size: 12 346 449
Fragments arranged by size:
1012 928 467 449 346 335 265 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 843 908 1150 107
Size: 736 65 242 3126
Fragments arranged by size:
3126 736 242 65
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 546 1053 1068 2119 2300 2463 546
Size: 507 15 1051 181 163 2252
Fragments arranged by size:
2252 1051 507 181 163 15
BtsI GCAGTG_nn'
Cuts at: 1412 1412
Size: 4169
ClaI AT'CG_AT
Cuts at: 776 776
Size: 4169
DraI TTT'AAA
Cuts at: 1168 1168
Size: 4169
DrdI GACnn_nn'nnGTC
Cuts at: 1875 2733 1875
Size: 858 3311
EaeI y'GGCC_r
Cuts at: 1754 1928 2319 2346 3691 1754
Size: 174 391 27 1345 2232
Fragments arranged by size:
2232 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1754 1754
Size: 4169
EarI CTCTTCn'nnn_
Cuts at: 317 389 679 2192 2402 317
Size: 72 290 1513 210 2084
Fragments arranged by size:
2084 1513 290 210 72
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2377 2687 2833 3979 2377
Size: 310 146 1146 2567
Fragments arranged by size:
2567 1146 310 146
EcoICRI GAG'CTC
Cuts at: 677 1101 1181 677
Size: 424 80 3665
Fragments arranged by size:
3665 424 80
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 301 524 663 728 1177 1994 2426 3173 3441
Size: 223 139 65 449 817 432 747 268
Cuts at: 3441 301
Size: 1029
Fragments arranged by size:
1029 817 747 449 432 268 223 139
65
EcoO109I rG'GnC_Cy
Cuts at: 213 224 472 603 849 900 1308 213
Size: 11 248 131 246 51 408 3074
Fragments arranged by size:
3074 408 248 246 131 51 11
EcoRI G'AATT_C
Cuts at: 1173 1173
Size: 4169
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2188 2188
Size: 4169
FspI TGC'GCA
Cuts at: 1950 1950
Size: 4169
HaeII r_GCGC'y
Cuts at: 431 1603 1851 2873 3852 431
Size: 1172 248 1022 979 748
Fragments arranged by size:
1172 1022 979 748 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 688 720 2011 2041 2043 2073 688
Size: 32 1291 30 2 30 2784
Fragments arranged by size:
2784 1291 32 30 30 2
HincII GTy'rAC
Cuts at: 1208 1208
Size: 4169
KasI G'GCGC_C
Cuts at: 1599 1847 1599
Size: 248 3921
KpnI G_GTAC'C
Cuts at: 484 1189 484
Size: 705 3464
MluI A'CGCG_T
Cuts at: 3602 3602
Size: 4169
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2840 3024 2840
Size: 184 3985
MscI TGG'CCA
Cuts at: 1930 1930
Size: 4169
MslI CAynn'nnrTG
Cuts at: 2158 2285 2158
Size: 127 4042
MspA1I CmG'CkG
Cuts at: 1280 1594 1954 2967 3212 1280
Size: 314 360 1013 245 2237
Fragments arranged by size:
2237 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2350 2350
Size: 4169
NarI GG'CG_CC
Cuts at: 1600 1848 1600
Size: 248 3921
NcoI C'CATG_G
Cuts at: 107 107
Size: 4169
NgoMIV G'CCGG_C
Cuts at: 2348 2348
Size: 4169
NspI r_CATG'y
Cuts at: 924 1165 1222 1722 2629 924
Size: 241 57 500 907 2464
Fragments arranged by size:
2464 907 500 241 57
PciI A'CATG_T
Cuts at: 920 2625 920
Size: 1705 2464
PfoI T'CCnGG_A
Cuts at: 679 679
Size: 4169
PmeI GTTT'AAAC
Cuts at: 1168 1168
Size: 4169
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 280 312 3308 3340 280
Size: 32 2996 32 1109
Fragments arranged by size:
2996 1109 32 32
PpuMI rG'GwC_Cy
Cuts at: 472 849 900 472
Size: 377 51 3741
Fragments arranged by size:
3741 377 51
PspOMI G'GGCC_C
Cuts at: 189 213 189
Size: 24 4145
PstI C_TGCA'G
Cuts at: 704 1216 1901 704
Size: 512 685 2972
Fragments arranged by size:
2972 685 512
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4169
PvuII CAG'CTG
Cuts at: 1594 1954 1594
Size: 360 3809
RsrII CG'GwC_CG
Cuts at: 2364 2364
Size: 4169
SacI G_AGCT'C
Cuts at: 679 1103 1183 679
Size: 424 80 3665
Fragments arranged by size:
3665 424 80
SalI G'TCGA_C
Cuts at: 1206 1206
Size: 4169
SapI GCTCTTCn'nnn_
Cuts at: 679 2192 2402 679
Size: 1513 210 2446
Fragments arranged by size:
2446 1513 210
SbfI CC_TGCA'GG
Cuts at: 1216 1216
Size: 4169
ScaI AGT'ACT
Cuts at: 3686 3686
Size: 4169
SfcI C'TryA_G
Cuts at: 33 381 700 1212 1897 2890 3081 33
Size: 348 319 512 685 993 191 1121
Fragments arranged by size:
1121 993 685 512 348 319 191
SfoI GGC'GCC
Cuts at: 1601 1849 1601
Size: 248 3921
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4169
SmaI CCC'GGG
Cuts at: 188 218 820 1191 188
Size: 30 602 371 3166
Fragments arranged by size:
3166 602 371 30
SmlI C'TyrA_G
Cuts at: 620 1324 2239 2731 2993 620
Size: 704 915 492 262 1796
Fragments arranged by size:
1796 915 704 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4169
SphI G_CATG'C
Cuts at: 1222 1722 1222
Size: 500 3669
StyI C'CwwG_G
Cuts at: 107 984 1303 1657 107
Size: 877 319 354 2619
Fragments arranged by size:
2619 877 354 319
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1752 1885 2344 2553 1752
Size: 133 459 209 3368
Fragments arranged by size:
3368 459 209 133
TatI w'GTAC_w
Cuts at: 923 3684 923
Size: 2761 1408
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3686 3718 3686
Size: 32 4137
Tth111I GACn'n_nGTC
Cuts at: 1966 1966
Size: 4169
XbaI T'CTAG_A
Cuts at: 1200 1200
Size: 4169
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1664 1664
Size: 4169
XmaI C'CCGG_G
Cuts at: 186 216 818 1189 186
Size: 30 602 371 3166
Fragments arranged by size:
3166 602 371 30
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI ClaI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PfoI PmeI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI BaeI BbvCI BfrBI BglI BmgBI BmtI Bpu10I
BsiWI BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PmlI PshAI PsiI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI