Restriction map for
pF1KB6470
(Circular) (Six-base) MAPSORT of: pF1KB6470.seq Check: 6404 from: 1 to: 4175
pF1KB6470 4175 bp
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AccI GT'mk_AC
Cuts at: 1213 1213
Size: 4175
Acc65I G'GTAC_C
Cuts at: 738 1191 738
Size: 453 3722
AclI AA'CG_TT
Cuts at: 4015 4015
Size: 4175
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 162 2000 2432 3179 3447 162
Size: 1838 432 747 268 890
Fragments arranged by size:
1838 890 747 432 268
AflIII A'CryG_T
Cuts at: 857 1527 2631 3608 857
Size: 670 1104 977 1424
Fragments arranged by size:
1424 1104 977 670
AgeI A'CCGG_T
Cuts at: 2563 2563
Size: 4175
AlwNI CAG_nnn'CTG
Cuts at: 804 1480 3047 804
Size: 676 1567 1932
Fragments arranged by size:
1932 1567 676
ApaI G_GGCC'C
Cuts at: 337 384 394 337
Size: 47 10 4118
Fragments arranged by size:
4118 47 10
ApaLI G'TGCA_C
Cuts at: 2945 2945
Size: 4175
ApoI r'AATT_y
Cuts at: 1179 3528 3784 1179
Size: 2349 256 1570
Fragments arranged by size:
2349 1570 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4175
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4175
AvaI C'yCGr_G
Cuts at: 377 1195 377
Size: 818 3357
BamHI G'GATC_C
Cuts at: 1200 1200
Size: 4175
BanI G'GyrC_C
Cuts at: 738 1191 1605 1853 1888 738
Size: 453 414 248 35 3025
Fragments arranged by size:
3025 453 414 248 35
BanII G_rGCy'C
Cuts at: 337 384 394 558 647 881 1189 2219 337
Size: 47 10 164 89 234 308 1030 2293
Fragments arranged by size:
2293 1030 308 234 164 89 47 10
BbeI G_GCGC'C
Cuts at: 1609 1857 1609
Size: 248 3927
BbsI GAAGACnn'nnnn_
Cuts at: 275 1153 275
Size: 878 3297
BciVI GTATCCnnnnn_n'
Cuts at: 1063 2073 2834 4175 1063
Size: 1010 761 1341 1063
Fragments arranged by size:
1341 1063 1010 761
BclI T'GATC_A
Cuts at: 1695 1695
Size: 4175
BglI GCCn_nnn'nGGC
Cuts at: 1162 1162
Size: 4175
BglII A'GATC_T
Cuts at: 1690 3727 1690
Size: 2037 2138
BlpI GC'TnA_GC
Cuts at: 1287 1287
Size: 4175
Bme1580I G_kGCm'C
Cuts at: 317 337 384 394 1800 1893 2949 317
Size: 20 47 10 1406 93 1056 1543
Fragments arranged by size:
1543 1406 1056 93 47 20 10
BmrI ACTGGGnnnn_n'
Cuts at: 1387 1468 1803 3947 1387
Size: 81 335 2144 1615
Fragments arranged by size:
2144 1615 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2542 2542
Size: 4175
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 323 1069 323
Size: 746 3429
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 588 1351 2230 2722 3020 588
Size: 763 879 492 298 1743
Fragments arranged by size:
1743 879 763 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3879 3879
Size: 4175
BsaAI yAC'GTr
Cuts at: 1528 1528
Size: 4175
BsaBI GATnn'nnATC
Cuts at: 6 1689 3726 6
Size: 1683 2037 455
Fragments arranged by size:
2037 1683 455
BsaHI Gr'CG_yC
Cuts at: 692 1606 1854 2556 4052 692
Size: 914 248 702 1496 815
Fragments arranged by size:
1496 914 815 702 248
BsaWI w'CCGG_w
Cuts at: 671 1364 1885 2563 2837 2984 3395 671
Size: 693 521 678 274 147 411 1451
Fragments arranged by size:
1451 693 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 585 585
Size: 4175
BseYI C'CCAG_C
Cuts at: 387 393 557 1600 2358 2935 3431 387
Size: 6 164 1043 758 577 496 1131
Fragments arranged by size:
1131 1043 758 577 496 164 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 683 683
Size: 4175
BsiEI CG_ry'CG
Cuts at: 104 1717 1763 2971 104
Size: 1613 46 1208 1308
Fragments arranged by size:
1613 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1189 1967 2157 2949 1189
Size: 778 190 792 2415
Fragments arranged by size:
2415 792 778 190
BsmI GAATG_Cn'
Cuts at: 362 927 362
Size: 565 3610
Bsp1286I G_dGCh'C
Cuts at: 317 337 384 394 558 647 881 1189 1800
Size: 20 47 10 164 89 234 308 611
Cuts at: 1800 1893 1967 2157 2219 2949 317
Size: 93 74 190 62 730 1543
Fragments arranged by size:
1543 730 611 308 234 190 164 93
89 74 62 47 20 10
BspEI T'CCGG_A
Cuts at: 3395 3395
Size: 4175
BspHI T'CATG_A
Cuts at: 3351 3351
Size: 4175
BspMI ACCTGCnnnn'nnnn_
Cuts at: 821 1225 1439 1741 2122 821
Size: 404 214 302 381 2874
Fragments arranged by size:
2874 404 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2467 2521 4005 4170 50
Size: 2417 54 1484 165 55
Fragments arranged by size:
2417 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 326 2087 326
Size: 1761 2414
BsrFI r'CCGG_y
Cuts at: 199 346 2173 2354 2563 199
Size: 147 1827 181 209 1811
Fragments arranged by size:
1827 1811 209 181 147
BssSI C'ACGA_G
Cuts at: 2446 2804 2446
Size: 358 3817
BstAPI GCAn_nnn'nTGC
Cuts at: 368 1552 368
Size: 1184 2991
BstBI TT'CG_AA
Cuts at: 2536 2536
Size: 4175
BstXI CCAn_nnnn'nTGG
Cuts at: 114 114
Size: 4175
BstYI r'GATC_y
Cuts at: 1 698 1200 1667 1690 2025 2271 3283 3369
Size: 697 502 467 23 335 246 1012 86
Cuts at: 3369 3381 3727 1
Size: 12 346 449
Fragments arranged by size:
1012 697 502 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 217 578 107
Size: 110 361 3704
Fragments arranged by size:
3704 361 110
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2125 2306 2469 2125
Size: 181 163 3831
Fragments arranged by size:
3831 181 163
BtsI GCAGTG_nn'
Cuts at: 886 1418 886
Size: 532 3643
DraI TTT'AAA
Cuts at: 1174 1174
Size: 4175
DraIII CAC_nnn'GTG
Cuts at: 918 918
Size: 4175
DrdI GACnn_nn'nnGTC
Cuts at: 1881 2739 1881
Size: 858 3317
EaeI y'GGCC_r
Cuts at: 201 1163 1760 1934 2325 2352 3697 201
Size: 962 597 174 391 27 1345 679
Fragments arranged by size:
1345 962 679 597 391 174 27
EagI C'GGCC_G
Cuts at: 1760 1760
Size: 4175
EarI CTCTTCn'nnn_
Cuts at: 500 2198 2408 500
Size: 1698 210 2267
Fragments arranged by size:
2267 1698 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2383 2693 2839 3985 2383
Size: 310 146 1146 2573
Fragments arranged by size:
2573 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1187 1187
Size: 4175
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 162 323 1069 2000 2432 3179 3447 162
Size: 161 746 931 432 747 268 890
Fragments arranged by size:
931 890 747 746 432 268 161
EcoO109I rG'GnC_Cy
Cuts at: 186 334 381 1314 186
Size: 148 47 933 3047
Fragments arranged by size:
3047 933 148 47
EcoRI G'AATT_C
Cuts at: 1179 1179
Size: 4175
EcoRV GAT'ATC
Cuts at: 990 990
Size: 4175
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 511 2194 511
Size: 1683 2492
FspI TGC'GCA
Cuts at: 1956 1956
Size: 4175
HaeII r_GCGC'y
Cuts at: 200 805 1609 1857 2879 3858 200
Size: 605 804 248 1022 979 517
Fragments arranged by size:
1022 979 804 605 517 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 500 532 939 971 2017 2047 2049 2079 500
Size: 32 407 32 1046 30 2 30 2596
Fragments arranged by size:
2596 1046 407 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1214 1214
Size: 4175
KasI G'GCGC_C
Cuts at: 1605 1853 1605
Size: 248 3927
KpnI G_GTAC'C
Cuts at: 742 1195 742
Size: 453 3722
MluI A'CGCG_T
Cuts at: 3608 3608
Size: 4175
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 395 2846 3030 395
Size: 2451 184 1540
Fragments arranged by size:
2451 1540 184
MscI TGG'CCA
Cuts at: 1165 1936 1165
Size: 771 3404
MslI CAynn'nnrTG
Cuts at: 112 1078 2164 2291 112
Size: 966 1086 127 1996
Fragments arranged by size:
1996 1086 966 127
MspA1I CmG'CkG
Cuts at: 659 1286 1600 1960 2973 3218 659
Size: 627 314 360 1013 245 1616
Fragments arranged by size:
1616 1013 627 360 314 245
NaeI GCC'GGC
Cuts at: 201 2356 201
Size: 2155 2020
NarI GG'CG_CC
Cuts at: 1606 1854 1606
Size: 248 3927
NcoI C'CATG_G
Cuts at: 107 578 107
Size: 471 3704
NgoMIV G'CCGG_C
Cuts at: 199 2354 199
Size: 2155 2020
NspI r_CATG'y
Cuts at: 1228 1728 2635 1228
Size: 500 907 2768
Fragments arranged by size:
2768 907 500
PasI CC'CwG_GG
Cuts at: 783 783
Size: 4175
PciI A'CATG_T
Cuts at: 2631 2631
Size: 4175
PfoI T'CCnGG_A
Cuts at: 339 339
Size: 4175
PmeI GTTT'AAAC
Cuts at: 1174 1174
Size: 4175
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 844 876 3314 3346 844
Size: 32 2438 32 1673
Fragments arranged by size:
2438 1673 32 32
PspOMI G'GGCC_C
Cuts at: 333 380 390 333
Size: 47 10 4118
Fragments arranged by size:
4118 47 10
PstI C_TGCA'G
Cuts at: 818 1222 1907 818
Size: 404 685 3086
Fragments arranged by size:
3086 685 404
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4175
PvuII CAG'CTG
Cuts at: 1600 1960 1600
Size: 360 3815
RsrII CG'GwC_CG
Cuts at: 2370 2370
Size: 4175
SacI G_AGCT'C
Cuts at: 1189 1189
Size: 4175
SalI G'TCGA_C
Cuts at: 1212 1212
Size: 4175
SapI GCTCTTCn'nnn_
Cuts at: 2198 2408 2198
Size: 210 3965
SbfI CC_TGCA'GG
Cuts at: 1222 1222
Size: 4175
ScaI AGT'ACT
Cuts at: 3692 3692
Size: 4175
SfcI C'TryA_G
Cuts at: 33 814 1218 1903 2896 3087 33
Size: 781 404 685 993 191 1121
Fragments arranged by size:
1121 993 781 685 404 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 1162 1162
Size: 4175
SfoI GGC'GCC
Cuts at: 1607 1855 1607
Size: 248 3927
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4175
SmaI CCC'GGG
Cuts at: 379 1197 379
Size: 818 3357
SmlI C'TyrA_G
Cuts at: 567 1330 2245 2737 2999 567
Size: 763 915 492 262 1743
Fragments arranged by size:
1743 915 763 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4175
SphI G_CATG'C
Cuts at: 1228 1728 1228
Size: 500 3675
SrfI GCCC'GGGC
Cuts at: 379 379
Size: 4175
StuI AGG'CCT
Cuts at: 1124 1124
Size: 4175
StyI C'CwwG_G
Cuts at: 107 578 1309 1663 107
Size: 471 731 354 2619
Fragments arranged by size:
2619 731 471 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 562 703 899 1758 1891 2350 2559 562
Size: 141 196 859 133 459 209 2178
Fragments arranged by size:
2178 859 459 209 196 141 133
TatI w'GTAC_w
Cuts at: 3690 3690
Size: 4175
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3692 3724 3692
Size: 32 4143
Tth111I GACn'n_nGTC
Cuts at: 1972 1972
Size: 4175
XbaI T'CTAG_A
Cuts at: 1206 1206
Size: 4175
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1670 1670
Size: 4175
XmaI C'CCGG_G
Cuts at: 377 1195 377
Size: 818 3357
XmnI GAAnn'nnTTC
Cuts at: 859 859
Size: 4175
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PfoI PmeI PpiI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfiI SfoI SgfI SmaI SmlI
SpeI SphI SrfI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI
Bpu10I BsaXI BsiWI BsmBI BsrGI BssHII BstEII BstZ17I
Bsu36I ClaI CspCI EcoNI FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PflMI
PmlI PpuMI PshAI PsiI PspXI PsrI SacII SanDI
SexAI SgrAI SnaBI SspI SwaI XhoI ZraI