Restriction map for
pF1KB6472
(Circular) (Six-base) MAPSORT of: pF1KB6472.seq Check: 1214 from: 1 to: 4178
pF1KB6472 4178 bp
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AccI GT'mk_AC
Cuts at: 326 1216 326
Size: 890 3288
Acc65I G'GTAC_C
Cuts at: 1194 1194
Size: 4178
AclI AA'CG_TT
Cuts at: 4018 4018
Size: 4178
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1174 2003 2435 3182 3450 1174
Size: 829 432 747 268 1902
Fragments arranged by size:
1902 829 747 432 268
AfeI AGC'GCT
Cuts at: 151 151
Size: 4178
AflIII A'CryG_T
Cuts at: 893 1530 2634 3611 893
Size: 637 1104 977 1460
Fragments arranged by size:
1460 1104 977 637
AgeI A'CCGG_T
Cuts at: 2566 2566
Size: 4178
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 446 456 446
Size: 10 4168
AlwNI CAG_nnn'CTG
Cuts at: 1483 3050 1483
Size: 1567 2611
ApaLI G'TGCA_C
Cuts at: 2948 2948
Size: 4178
ApoI r'AATT_y
Cuts at: 1182 3531 3787 1182
Size: 2349 256 1573
Fragments arranged by size:
2349 1573 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4178
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4178
AvaI C'yCGr_G
Cuts at: 1198 1198
Size: 4178
BamHI G'GATC_C
Cuts at: 743 1203 743
Size: 460 3718
BanI G'GyrC_C
Cuts at: 1194 1608 1856 1891 1194
Size: 414 248 35 3481
Fragments arranged by size:
3481 414 248 35
BanII G_rGCy'C
Cuts at: 1192 2222 1192
Size: 1030 3148
BbeI G_GCGC'C
Cuts at: 1612 1860 1612
Size: 248 3930
BbvCI CC'TCA_GC
Cuts at: 815 815
Size: 4178
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 547 581 547
Size: 34 4144
BciVI GTATCCnnnnn_n'
Cuts at: 2076 2837 4178 2076
Size: 761 1341 2076
Fragments arranged by size:
2076 1341 761
BclI T'GATC_A
Cuts at: 1698 1698
Size: 4178
BfrBI ATG'CAT
Cuts at: 840 840
Size: 4178
BglII A'GATC_T
Cuts at: 1064 1693 3730 1064
Size: 629 2037 1512
Fragments arranged by size:
2037 1512 629
BlpI GC'TnA_GC
Cuts at: 1290 1290
Size: 4178
Bme1580I G_kGCm'C
Cuts at: 1803 1896 2952 1803
Size: 93 1056 3029
Fragments arranged by size:
3029 1056 93
BmgBI CAC'GTC
Cuts at: 896 896
Size: 4178
BmrI ACTGGGnnnn_n'
Cuts at: 1390 1471 1806 3950 1390
Size: 81 335 2144 1618
Fragments arranged by size:
2144 1618 335 81
BmtI G_CTAG'C
Cuts at: 814 814
Size: 4178
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2545 2545
Size: 4178
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 387 607 387
Size: 220 3958
Bpu10I CC'TnA_GC
Cuts at: 815 815
Size: 4178
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 522 810 1354 2233 2725 3023 522
Size: 288 544 879 492 298 1677
Fragments arranged by size:
1677 879 544 492 298 288
BsaI GGTCTCn'nnnn_
Cuts at: 3882 3882
Size: 4178
BsaAI yAC'GTr
Cuts at: 1082 1531 1082
Size: 449 3729
BsaBI GATnn'nnATC
Cuts at: 6 268 1692 3729 6
Size: 262 1424 2037 455
Fragments arranged by size:
2037 1424 455 262
BsaHI Gr'CG_yC
Cuts at: 416 485 761 1121 1609 1857 2559 4055 416
Size: 69 276 360 488 248 702 1496 539
Fragments arranged by size:
1496 702 539 488 360 276 248 69
BsaWI w'CCGG_w
Cuts at: 491 1367 1888 2566 2840 2987 3398 491
Size: 876 521 678 274 147 411 1271
Fragments arranged by size:
1271 876 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 196 226 196
Size: 30 4148
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 310 466 838 949 310
Size: 156 372 111 3539
Fragments arranged by size:
3539 372 156 111
BseYI C'CCAG_C
Cuts at: 229 1603 2361 2938 3434 229
Size: 1374 758 577 496 973
Fragments arranged by size:
1374 973 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 99 426 538 1126 99
Size: 327 112 588 3151
Fragments arranged by size:
3151 588 327 112
BsiEI CG_ry'CG
Cuts at: 104 146 1720 1766 2974 104
Size: 42 1574 46 1208 1308
Fragments arranged by size:
1574 1308 1208 46 42
BsiHKAI G_wGCw'C
Cuts at: 250 467 1192 1970 2160 2952 250
Size: 217 725 778 190 792 1476
Fragments arranged by size:
1476 792 778 725 217 190
BsmI GAATG_Cn'
Cuts at: 307 307
Size: 4178
Bsp1286I G_dGCh'C
Cuts at: 250 467 1192 1803 1896 1970 2160 2222 2952
Size: 217 725 611 93 74 190 62 730
Cuts at: 2952 250
Size: 1476
Fragments arranged by size:
1476 730 725 611 217 190 93 74
62
BspEI T'CCGG_A
Cuts at: 491 3398 491
Size: 2907 1271
BspHI T'CATG_A
Cuts at: 3354 3354
Size: 4178
BspMI ACCTGCnnnn'nnnn_
Cuts at: 182 386 1228 1442 1744 2125 182
Size: 204 842 214 302 381 2235
Fragments arranged by size:
2235 842 381 302 214 204
BsrBI CCG'CTC
Cuts at: 50 409 536 732 2470 2524 4008 4173 50
Size: 359 127 196 1738 54 1484 165 55
Fragments arranged by size:
1738 1484 359 196 165 127 55 54
BsrDI GCAATG_nn'
Cuts at: 2090 2090
Size: 4178
BsrFI r'CCGG_y
Cuts at: 305 2176 2357 2566 305
Size: 1871 181 209 1917
Fragments arranged by size:
1917 1871 209 181
BssSI C'ACGA_G
Cuts at: 277 2449 2807 277
Size: 2172 358 1648
Fragments arranged by size:
2172 1648 358
BstAPI GCAn_nnn'nTGC
Cuts at: 462 630 1555 462
Size: 168 925 3085
Fragments arranged by size:
3085 925 168
BstBI TT'CG_AA
Cuts at: 2539 2539
Size: 4178
BstXI CCAn_nnnn'nTGG
Cuts at: 283 283
Size: 4178
BstYI r'GATC_y
Cuts at: 1 743 1064 1203 1670 1693 2028 2274 3286
Size: 742 321 139 467 23 335 246 1012
Cuts at: 3286 3372 3384 3730 1
Size: 86 12 346 449
Fragments arranged by size:
1012 742 467 449 346 335 321 246
139 86 23 12
BtgI C'CryG_G
Cuts at: 107 368 510 653 107
Size: 261 142 143 3632
Fragments arranged by size:
3632 261 143 142
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2128 2309 2472 2128
Size: 181 163 3834
Fragments arranged by size:
3834 181 163
BtsI GCAGTG_nn'
Cuts at: 747 1421 747
Size: 674 3504
DraI TTT'AAA
Cuts at: 1177 1177
Size: 4178
DraIII CAC_nnn'GTG
Cuts at: 604 604
Size: 4178
DrdI GACnn_nn'nnGTC
Cuts at: 1884 2742 1884
Size: 858 3320
EaeI y'GGCC_r
Cuts at: 143 353 531 1763 1937 2328 2355 3700 143
Size: 210 178 1232 174 391 27 1345 621
Fragments arranged by size:
1345 1232 621 391 210 178 174 27
EagI C'GGCC_G
Cuts at: 143 1763 143
Size: 1620 2558
EarI CTCTTCn'nnn_
Cuts at: 238 913 1174 2201 2411 238
Size: 675 261 1027 210 2005
Fragments arranged by size:
2005 1027 675 261 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2386 2696 2842 3988 2386
Size: 310 146 1146 2576
Fragments arranged by size:
2576 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1190 1190
Size: 4178
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 387 607 1174 2003 2435 3182 3450 387
Size: 220 567 829 432 747 268 1115
Fragments arranged by size:
1115 829 747 567 432 268 220
EcoNI CCTnn'n_nnAGG
Cuts at: 178 931 178
Size: 753 3425
EcoO109I rG'GnC_Cy
Cuts at: 687 924 1035 1317 687
Size: 237 111 282 3548
Fragments arranged by size:
3548 282 237 111
EcoRI G'AATT_C
Cuts at: 1182 1182
Size: 4178
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2197 2197
Size: 4178
FspI TGC'GCA
Cuts at: 1959 1959
Size: 4178
HaeII r_GCGC'y
Cuts at: 128 153 797 1612 1860 2882 3861 128
Size: 25 644 815 248 1022 979 445
Fragments arranged by size:
1022 979 815 644 445 248 25
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 274 306 463 495 2020 2050 2052 2082 274
Size: 32 157 32 1525 30 2 30 2370
Fragments arranged by size:
2370 1525 157 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1217 1217
Size: 4178
KasI G'GCGC_C
Cuts at: 1608 1856 1608
Size: 248 3930
KpnI G_GTAC'C
Cuts at: 1198 1198
Size: 4178
MluI A'CGCG_T
Cuts at: 3611 3611
Size: 4178
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2849 3033 2849
Size: 184 3994
MscI TGG'CCA
Cuts at: 355 1939 355
Size: 1584 2594
MslI CAynn'nnrTG
Cuts at: 281 2167 2294 281
Size: 1886 127 2165
Fragments arranged by size:
2165 1886 127
MspA1I CmG'CkG
Cuts at: 565 723 1289 1603 1963 2976 3221 565
Size: 158 566 314 360 1013 245 1522
Fragments arranged by size:
1522 1013 566 360 314 245 158
NaeI GCC'GGC
Cuts at: 307 2359 307
Size: 2052 2126
NarI GG'CG_CC
Cuts at: 1609 1857 1609
Size: 248 3930
NcoI C'CATG_G
Cuts at: 107 368 510 107
Size: 261 142 3775
Fragments arranged by size:
3775 261 142
NgoMIV G'CCGG_C
Cuts at: 305 2357 305
Size: 2052 2126
NheI G'CTAG_C
Cuts at: 810 810
Size: 4178
NsiI A_TGCA'T
Cuts at: 842 842
Size: 4178
NspI r_CATG'y
Cuts at: 840 1231 1731 2638 840
Size: 391 500 907 2380
Fragments arranged by size:
2380 907 500 391
PciI A'CATG_T
Cuts at: 2634 2634
Size: 4178
PmeI GTTT'AAAC
Cuts at: 1177 1177
Size: 4178
PmlI CAC'GTG
Cuts at: 1082 1082
Size: 4178
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3317 3349 3317
Size: 32 4146
PpuMI rG'GwC_Cy
Cuts at: 687 924 1035 687
Size: 237 111 3830
Fragments arranged by size:
3830 237 111
PstI C_TGCA'G
Cuts at: 383 1225 1910 383
Size: 842 685 2651
Fragments arranged by size:
2651 842 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4178
PvuII CAG'CTG
Cuts at: 565 1603 1963 565
Size: 1038 360 2780
Fragments arranged by size:
2780 1038 360
RsrII CG'GwC_CG
Cuts at: 2373 2373
Size: 4178
SacI G_AGCT'C
Cuts at: 1192 1192
Size: 4178
SalI G'TCGA_C
Cuts at: 1215 1215
Size: 4178
SapI GCTCTTCn'nnn_
Cuts at: 238 2201 2411 238
Size: 1963 210 2005
Fragments arranged by size:
2005 1963 210
SbfI CC_TGCA'GG
Cuts at: 1225 1225
Size: 4178
ScaI AGT'ACT
Cuts at: 3695 3695
Size: 4178
SfcI C'TryA_G
Cuts at: 33 327 379 1221 1906 2899 3090 33
Size: 294 52 842 685 993 191 1121
Fragments arranged by size:
1121 993 842 685 294 191 52
SfoI GGC'GCC
Cuts at: 1610 1858 1610
Size: 248 3930
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4178
SmaI CCC'GGG
Cuts at: 1200 1200
Size: 4178
SmlI C'TyrA_G
Cuts at: 537 789 1333 2248 2740 3002 537
Size: 252 544 915 492 262 1713
Fragments arranged by size:
1713 915 544 492 262 252
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4178
SphI G_CATG'C
Cuts at: 840 1231 1731 840
Size: 391 500 3287
Fragments arranged by size:
3287 500 391
StuI AGG'CCT
Cuts at: 523 523
Size: 4178
StyI C'CwwG_G
Cuts at: 107 368 510 1058 1312 1666 107
Size: 261 142 548 254 354 2619
Fragments arranged by size:
2619 548 354 261 254 142
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 214 703 1761 1894 2353 2562 214
Size: 489 1058 133 459 209 1830
Fragments arranged by size:
1830 1058 489 459 209 133
TatI w'GTAC_w
Cuts at: 977 3693 977
Size: 2716 1462
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 936 968 3695 3727 936
Size: 32 2727 32 1387
Fragments arranged by size:
2727 1387 32 32
Tth111I GACn'n_nGTC
Cuts at: 1123 1975 1123
Size: 852 3326
XbaI T'CTAG_A
Cuts at: 1209 1209
Size: 4178
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 660 1673 660
Size: 1013 3165
XmaI C'CCGG_G
Cuts at: 1198 1198
Size: 4178
XmnI GAAnn'nnTTC
Cuts at: 856 856
Size: 4178
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BcgI BciVI BclI BfrBI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NheI NsiI NspI PciI PmeI PmlI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AloI ApaI AscI
AvrII BaeI BbsI BglI BsiWI BsmBI BsrGI BssHII
BstEII BstZ17I Bsu36I ClaI CspCI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NotI NruI PacI PasI
PflMI PfoI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI ZraI