Restriction map for
pF1KB6474
(Circular) (Six-base) MAPSORT of: pF1KB6474.seq Check: 4015 from: 1 to: 4178
pF1KB6474 4178 bp
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AccI GT'mk_AC
Cuts at: 725 1216 725
Size: 491 3687
Acc65I G'GTAC_C
Cuts at: 1194 1194
Size: 4178
AclI AA'CG_TT
Cuts at: 4018 4018
Size: 4178
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 141 284 2003 2435 3182 3450 141
Size: 143 1719 432 747 268 869
Fragments arranged by size:
1719 869 747 432 268 143
AflIII A'CryG_T
Cuts at: 1530 2634 3611 1530
Size: 1104 977 2097
Fragments arranged by size:
2097 1104 977
AgeI A'CCGG_T
Cuts at: 2566 2566
Size: 4178
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1105 1105
Size: 4178
AlwNI CAG_nnn'CTG
Cuts at: 1483 3050 1483
Size: 1567 2611
ApaLI G'TGCA_C
Cuts at: 2948 2948
Size: 4178
ApoI r'AATT_y
Cuts at: 424 541 1182 3531 3787 424
Size: 117 641 2349 256 815
Fragments arranged by size:
2349 815 641 256 117
AseI AT'TA_AT
Cuts at: 20 20
Size: 4178
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4178
AvaI C'yCGr_G
Cuts at: 1198 1198
Size: 4178
BamHI G'GATC_C
Cuts at: 927 1203 927
Size: 276 3902
BanI G'GyrC_C
Cuts at: 1194 1608 1856 1891 1194
Size: 414 248 35 3481
Fragments arranged by size:
3481 414 248 35
BanII G_rGCy'C
Cuts at: 1172 1192 2222 1172
Size: 20 1030 3128
Fragments arranged by size:
3128 1030 20
BbeI G_GCGC'C
Cuts at: 1612 1860 1612
Size: 248 3930
BbsI GAAGACnn'nnnn_
Cuts at: 164 272 164
Size: 108 4070
BciVI GTATCCnnnnn_n'
Cuts at: 329 2076 2837 4178 329
Size: 1747 761 1341 329
Fragments arranged by size:
1747 1341 761 329
BclI T'GATC_A
Cuts at: 654 1698 654
Size: 1044 3134
BglII A'GATC_T
Cuts at: 1693 3730 1693
Size: 2037 2141
BlpI GC'TnA_GC
Cuts at: 1290 1290
Size: 4178
Bme1580I G_kGCm'C
Cuts at: 1803 1896 2952 1803
Size: 93 1056 3029
Fragments arranged by size:
3029 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1390 1471 1806 3950 1390
Size: 81 335 2144 1618
Fragments arranged by size:
2144 1618 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2545 2545
Size: 4178
Bpu10I CC'TnA_GC
Cuts at: 632 632
Size: 4178
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1354 2233 2725 3023 1354
Size: 879 492 298 2509
Fragments arranged by size:
2509 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 935 3882 935
Size: 2947 1231
BsaAI yAC'GTr
Cuts at: 1531 1531
Size: 4178
BsaBI GATnn'nnATC
Cuts at: 6 1692 3729 6
Size: 1686 2037 455
Fragments arranged by size:
2037 1686 455
BsaHI Gr'CG_yC
Cuts at: 1609 1857 2559 4055 1609
Size: 248 702 1496 1732
Fragments arranged by size:
1732 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1367 1888 2566 2840 2987 3398 1367
Size: 521 678 274 147 411 2147
Fragments arranged by size:
2147 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 861 891 861
Size: 30 4148
BseYI C'CCAG_C
Cuts at: 1603 2361 2938 3434 1603
Size: 758 577 496 2347
Fragments arranged by size:
2347 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1720 1766 2974 104
Size: 1616 46 1208 1308
Fragments arranged by size:
1616 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 638 1172 1192 1970 2160 2952 638
Size: 534 20 778 190 792 1864
Fragments arranged by size:
1864 792 778 534 190 20
Bsp1286I G_dGCh'C
Cuts at: 638 1172 1192 1803 1896 1970 2160 2222 2952
Size: 534 20 611 93 74 190 62 730
Cuts at: 2952 638
Size: 1864
Fragments arranged by size:
1864 730 611 534 190 93 74 62
20
BspEI T'CCGG_A
Cuts at: 3398 3398
Size: 4178
BspHI T'CATG_A
Cuts at: 3354 3354
Size: 4178
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1228 1442 1744 2125 1228
Size: 214 302 381 3281
Fragments arranged by size:
3281 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2470 2524 4008 4173 50
Size: 2420 54 1484 165 55
Fragments arranged by size:
2420 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2090 2090
Size: 4178
BsrFI r'CCGG_y
Cuts at: 2176 2357 2566 2176
Size: 181 209 3788
Fragments arranged by size:
3788 209 181
BsrGI T'GTAC_A
Cuts at: 1115 1115
Size: 4178
BssSI C'ACGA_G
Cuts at: 907 2449 2807 907
Size: 1542 358 2278
Fragments arranged by size:
2278 1542 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1555 1555
Size: 4178
BstBI TT'CG_AA
Cuts at: 1023 2539 1023
Size: 1516 2662
BstXI CCAn_nnnn'nTGG
Cuts at: 967 967
Size: 4178
BstYI r'GATC_y
Cuts at: 1 387 927 1203 1670 1693 2028 2274 3286
Size: 386 540 276 467 23 335 246 1012
Cuts at: 3286 3372 3384 3730 1
Size: 86 12 346 449
Fragments arranged by size:
1012 540 467 449 386 346 335 276
246 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4178
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2128 2309 2472 2128
Size: 181 163 3834
Fragments arranged by size:
3834 181 163
BtsI GCAGTG_nn'
Cuts at: 1421 1421
Size: 4178
DraI TTT'AAA
Cuts at: 540 1177 540
Size: 637 3541
DrdI GACnn_nn'nnGTC
Cuts at: 1884 2742 1884
Size: 858 3320
EaeI y'GGCC_r
Cuts at: 1763 1937 2328 2355 3700 1763
Size: 174 391 27 1345 2241
Fragments arranged by size:
2241 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1763 1763
Size: 4178
EarI CTCTTCn'nnn_
Cuts at: 160 2201 2411 160
Size: 2041 210 1927
Fragments arranged by size:
2041 1927 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2386 2696 2842 3988 2386
Size: 310 146 1146 2576
Fragments arranged by size:
2576 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1170 1190 1170
Size: 20 4158
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 141 284 2003 2435 3182 3450 141
Size: 143 1719 432 747 268 869
Fragments arranged by size:
1719 869 747 432 268 143
EcoNI CCTnn'n_nnAGG
Cuts at: 935 935
Size: 4178
EcoO109I rG'GnC_Cy
Cuts at: 628 1317 628
Size: 689 3489
EcoRI G'AATT_C
Cuts at: 1182 1182
Size: 4178
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2197 2197
Size: 4178
FspI TGC'GCA
Cuts at: 1959 1959
Size: 4178
HaeII r_GCGC'y
Cuts at: 1612 1860 2882 3861 1612
Size: 248 1022 979 1929
Fragments arranged by size:
1929 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 137 169 505 537 861 893 904 936 2020
Size: 32 336 32 324 32 11 32 1084
Cuts at: 2020 2050 2052 2082 137
Size: 30 2 30 2233
Fragments arranged by size:
2233 1084 336 324 32 32 32 32
30 30 11 2
HincII GTy'rAC
Cuts at: 1217 1217
Size: 4178
KasI G'GCGC_C
Cuts at: 1608 1856 1608
Size: 248 3930
KpnI G_GTAC'C
Cuts at: 1198 1198
Size: 4178
MluI A'CGCG_T
Cuts at: 3611 3611
Size: 4178
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 380 415 933 2849 3033 380
Size: 35 518 1916 184 1525
Fragments arranged by size:
1916 1525 518 184 35
MscI TGG'CCA
Cuts at: 1939 1939
Size: 4178
MslI CAynn'nnrTG
Cuts at: 512 641 848 922 965 2167 2294 512
Size: 129 207 74 43 1202 127 2396
Fragments arranged by size:
2396 1202 207 129 127 74 43
MspA1I CmG'CkG
Cuts at: 1289 1603 1963 2976 3221 1289
Size: 314 360 1013 245 2246
Fragments arranged by size:
2246 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2359 2359
Size: 4178
NarI GG'CG_CC
Cuts at: 1609 1857 1609
Size: 248 3930
NcoI C'CATG_G
Cuts at: 107 107
Size: 4178
NgoMIV G'CCGG_C
Cuts at: 2357 2357
Size: 4178
NspI r_CATG'y
Cuts at: 862 1231 1731 2638 862
Size: 369 500 907 2402
Fragments arranged by size:
2402 907 500 369
PciI A'CATG_T
Cuts at: 2634 2634
Size: 4178
PfoI T'CCnGG_A
Cuts at: 321 351 321
Size: 30 4148
PmeI GTTT'AAAC
Cuts at: 1177 1177
Size: 4178
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3317 3349 3317
Size: 32 4146
PpuMI rG'GwC_Cy
Cuts at: 628 628
Size: 4178
PstI C_TGCA'G
Cuts at: 1225 1910 1225
Size: 685 3493
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4178
PvuII CAG'CTG
Cuts at: 1603 1963 1603
Size: 360 3818
RsrII CG'GwC_CG
Cuts at: 2373 2373
Size: 4178
SacI G_AGCT'C
Cuts at: 1172 1192 1172
Size: 20 4158
SalI G'TCGA_C
Cuts at: 1215 1215
Size: 4178
SapI GCTCTTCn'nnn_
Cuts at: 2201 2411 2201
Size: 210 3968
SbfI CC_TGCA'GG
Cuts at: 1225 1225
Size: 4178
ScaI AGT'ACT
Cuts at: 3695 3695
Size: 4178
SfcI C'TryA_G
Cuts at: 33 729 1221 1906 2899 3090 33
Size: 696 492 685 993 191 1121
Fragments arranged by size:
1121 993 696 685 492 191
SfoI GGC'GCC
Cuts at: 1610 1858 1610
Size: 248 3930
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4178
SmaI CCC'GGG
Cuts at: 1200 1200
Size: 4178
SmlI C'TyrA_G
Cuts at: 1333 2248 2740 3002 1333
Size: 915 492 262 2509
Fragments arranged by size:
2509 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4178
SphI G_CATG'C
Cuts at: 862 1231 1731 862
Size: 369 500 3309
Fragments arranged by size:
3309 500 369
StyI C'CwwG_G
Cuts at: 107 1312 1666 107
Size: 1205 354 2619
Fragments arranged by size:
2619 1205 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1761 1894 2353 2562 1761
Size: 133 459 209 3377
Fragments arranged by size:
3377 459 209 133
TatI w'GTAC_w
Cuts at: 1099 1115 3693 1099
Size: 16 2578 1584
Fragments arranged by size:
2578 1584 16
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3695 3727 3695
Size: 32 4146
Tth111I GACn'n_nGTC
Cuts at: 1975 1975
Size: 4178
XbaI T'CTAG_A
Cuts at: 1209 1209
Size: 4178
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 492 525 1673 492
Size: 33 1148 2997
Fragments arranged by size:
2997 1148 33
XmaI C'CCGG_G
Cuts at: 1198 1198
Size: 4178
XmnI GAAnn'nnTTC
Cuts at: 979 979
Size: 4178
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PfoI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI BpmI BseRI BsgI BsiWI BsmI BsmBI BssHII
BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PmlI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI ZraI