Restriction map for
pF1KB6475
(Circular) (Six-base) MAPSORT of: pF1KB6475.seq Check: 9148 from: 1 to: 4091
pF1KB6475 4091 bp
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1055 1055
Size: 4091
AccI GT'mk_AC
Cuts at: 1129 1129
Size: 4091
Acc65I G'GTAC_C
Cuts at: 1107 1107
Size: 4091
AclI AA'CG_TT
Cuts at: 3931 3931
Size: 4091
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 961 1916 2348 3095 3363 961
Size: 955 432 747 268 1689
Fragments arranged by size:
1689 955 747 432 268
AflIII A'CryG_T
Cuts at: 405 1443 2547 3524 405
Size: 1038 1104 977 972
Fragments arranged by size:
1104 1038 977 972
AgeI A'CCGG_T
Cuts at: 2479 2479
Size: 4091
AleI CACnn'nnGTG
Cuts at: 703 703
Size: 4091
AlwNI CAG_nnn'CTG
Cuts at: 451 1014 1396 2963 451
Size: 563 382 1567 1579
Fragments arranged by size:
1579 1567 563 382
ApaLI G'TGCA_C
Cuts at: 354 2861 354
Size: 2507 1584
ApoI r'AATT_y
Cuts at: 1095 3444 3700 1095
Size: 2349 256 1486
Fragments arranged by size:
2349 1486 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4091
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4091
AvaI C'yCGr_G
Cuts at: 1111 1111
Size: 4091
BamHI G'GATC_C
Cuts at: 1116 1116
Size: 4091
BanI G'GyrC_C
Cuts at: 172 305 1107 1521 1769 1804 172
Size: 133 802 414 248 35 2459
Fragments arranged by size:
2459 802 414 248 133 35
BanII G_rGCy'C
Cuts at: 1105 2135 1105
Size: 1030 3061
BbeI G_GCGC'C
Cuts at: 176 1525 1773 176
Size: 1349 248 2494
Fragments arranged by size:
2494 1349 248
BbsI GAAGACnn'nnnn_
Cuts at: 836 836
Size: 4091
BbvCI CC'TCA_GC
Cuts at: 301 301
Size: 4091
BciVI GTATCCnnnnn_n'
Cuts at: 1989 2750 4091 1989
Size: 761 1341 1989
Fragments arranged by size:
1989 1341 761
BclI T'GATC_A
Cuts at: 783 1611 783
Size: 828 3263
BglII A'GATC_T
Cuts at: 1606 3643 1606
Size: 2037 2054
BlpI GC'TnA_GC
Cuts at: 1203 1203
Size: 4091
Bme1580I G_kGCm'C
Cuts at: 358 461 990 1716 1809 2865 358
Size: 103 529 726 93 1056 1584
Fragments arranged by size:
1584 1056 726 529 103 93
BmgBI CAC'GTC
Cuts at: 807 807
Size: 4091
BmrI ACTGGGnnnn_n'
Cuts at: 1303 1384 1719 3863 1303
Size: 81 335 2144 1531
Fragments arranged by size:
2144 1531 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2458 2458
Size: 4091
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 331 473 667 735 331
Size: 142 194 68 3687
Fragments arranged by size:
3687 194 142 68
Bpu10I CC'TnA_GC
Cuts at: 301 301
Size: 4091
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1267 2146 2638 2936 1267
Size: 879 492 298 2422
Fragments arranged by size:
2422 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 307 3795 307
Size: 3488 603
BsaAI yAC'GTr
Cuts at: 1444 1444
Size: 4091
BsaBI GATnn'nnATC
Cuts at: 6 782 1605 3642 6
Size: 776 823 2037 455
Fragments arranged by size:
2037 823 776 455
BsaHI Gr'CG_yC
Cuts at: 173 1522 1770 2472 3968 173
Size: 1349 248 702 1496 296
Fragments arranged by size:
1496 1349 702 296 248
BsaWI w'CCGG_w
Cuts at: 1280 1801 2479 2753 2900 3311 1280
Size: 521 678 274 147 411 2060
Fragments arranged by size:
2060 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 274 274
Size: 4091
BseYI C'CCAG_C
Cuts at: 960 1054 1516 2274 2851 3347 960
Size: 94 462 758 577 496 1704
Fragments arranged by size:
1704 758 577 496 462 94
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 268 268
Size: 4091
BsiEI CG_ry'CG
Cuts at: 104 1633 1679 2887 104
Size: 1529 46 1208 1308
Fragments arranged by size:
1529 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 326 358 669 1105 1883 2073 2865 326
Size: 32 311 436 778 190 792 1552
Fragments arranged by size:
1552 792 778 436 311 190 32
BsmI GAATG_Cn'
Cuts at: 154 154
Size: 4091
Bsp1286I G_dGCh'C
Cuts at: 326 358 461 669 990 1105 1716 1809 1883
Size: 32 103 208 321 115 611 93 74
Cuts at: 1883 2073 2135 2865 326
Size: 190 62 730 1552
Fragments arranged by size:
1552 730 611 321 208 190 115 103
93 74 62 32
BspEI T'CCGG_A
Cuts at: 3311 3311
Size: 4091
BspHI T'CATG_A
Cuts at: 3267 3267
Size: 4091
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1055 1141 1355 1657 2038 1055
Size: 86 214 302 381 3108
Fragments arranged by size:
3108 381 302 214 86
BsrBI CCG'CTC
Cuts at: 50 273 2383 2437 3921 4086 50
Size: 223 2110 54 1484 165 55
Fragments arranged by size:
2110 1484 223 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2003 2003
Size: 4091
BsrFI r'CCGG_y
Cuts at: 253 2089 2270 2479 253
Size: 1836 181 209 1865
Fragments arranged by size:
1865 1836 209 181
BssSI C'ACGA_G
Cuts at: 2362 2720 2362
Size: 358 3733
BstAPI GCAn_nnn'nTGC
Cuts at: 1468 1468
Size: 4091
BstBI TT'CG_AA
Cuts at: 2452 2452
Size: 4091
BstEII G'GTnAC_C
Cuts at: 564 564
Size: 4091
BstXI CCAn_nnnn'nTGG
Cuts at: 432 705 432
Size: 273 3818
BstYI r'GATC_y
Cuts at: 1 708 1116 1583 1606 1941 2187 3199 3285
Size: 707 408 467 23 335 246 1012 86
Cuts at: 3285 3297 3643 1
Size: 12 346 449
Fragments arranged by size:
1012 707 467 449 408 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 704 107
Size: 597 3494
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 520 2041 2222 2385 520
Size: 1521 181 163 2226
Fragments arranged by size:
2226 1521 181 163
BtsI GCAGTG_nn'
Cuts at: 1334 1334
Size: 4091
ClaI AT'CG_AT
Cuts at: 914 914
Size: 4091
DraI TTT'AAA
Cuts at: 1090 1090
Size: 4091
DraIII CAC_nnn'GTG
Cuts at: 656 656
Size: 4091
DrdI GACnn_nn'nnGTC
Cuts at: 1797 2655 1797
Size: 858 3233
EaeI y'GGCC_r
Cuts at: 1676 1850 2241 2268 3613 1676
Size: 174 391 27 1345 2154
Fragments arranged by size:
2154 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1676 1676
Size: 4091
EarI CTCTTCn'nnn_
Cuts at: 2114 2324 2114
Size: 210 3881
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 172 2299 2609 2755 3901 172
Size: 2127 310 146 1146 362
Fragments arranged by size:
2127 1146 362 310 146
EcoICRI GAG'CTC
Cuts at: 1103 1103
Size: 4091
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 331 473 667 735 961 1916 2348 3095 3363
Size: 142 194 68 226 955 432 747 268
Cuts at: 3363 331
Size: 1059
Fragments arranged by size:
1059 955 747 432 268 226 194 142
68
EcoO109I rG'GnC_Cy
Cuts at: 1011 1230 1011
Size: 219 3872
EcoRI G'AATT_C
Cuts at: 1095 1095
Size: 4091
EcoRV GAT'ATC
Cuts at: 912 912
Size: 4091
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 952 2110 952
Size: 1158 2933
FspI TGC'GCA
Cuts at: 1872 1872
Size: 4091
HaeII r_GCGC'y
Cuts at: 176 1525 1773 2795 3774 176
Size: 1349 248 1022 979 493
Fragments arranged by size:
1349 1022 979 493 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1933 1963 1965 1995 1933
Size: 30 2 30 4029
Fragments arranged by size:
4029 30 30 2
HincII GTy'rAC
Cuts at: 1130 1130
Size: 4091
KasI G'GCGC_C
Cuts at: 172 1521 1769 172
Size: 1349 248 2494
Fragments arranged by size:
2494 1349 248
KpnI G_GTAC'C
Cuts at: 1111 1111
Size: 4091
MfeI C'AATT_G
Cuts at: 890 890
Size: 4091
MluI A'CGCG_T
Cuts at: 3524 3524
Size: 4091
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2762 2946 2762
Size: 184 3907
MscI TGG'CCA
Cuts at: 1852 1852
Size: 4091
MslI CAynn'nnrTG
Cuts at: 562 703 2080 2207 562
Size: 141 1377 127 2446
Fragments arranged by size:
2446 1377 141 127
MspA1I CmG'CkG
Cuts at: 575 1202 1516 1876 2889 3134 575
Size: 627 314 360 1013 245 1532
Fragments arranged by size:
1532 1013 627 360 314 245
NaeI GCC'GGC
Cuts at: 2272 2272
Size: 4091
NarI GG'CG_CC
Cuts at: 173 1522 1770 173
Size: 1349 248 2494
Fragments arranged by size:
2494 1349 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4091
NgoMIV G'CCGG_C
Cuts at: 2270 2270
Size: 4091
NruI TCG'CGA
Cuts at: 539 539
Size: 4091
NspI r_CATG'y
Cuts at: 270 409 1144 1644 2551 270
Size: 139 735 500 907 1810
Fragments arranged by size:
1810 907 735 500 139
PciI A'CATG_T
Cuts at: 405 2547 405
Size: 2142 1949
PflMI CCAn_nnn'nTGG
Cuts at: 451 1014 451
Size: 563 3528
PfoI T'CCnGG_A
Cuts at: 1007 1007
Size: 4091
PmeI GTTT'AAAC
Cuts at: 1090 1090
Size: 4091
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3230 3262 3230
Size: 32 4059
PpuMI rG'GwC_Cy
Cuts at: 1011 1011
Size: 4091
PstI C_TGCA'G
Cuts at: 1138 1823 1138
Size: 685 3406
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4091
PvuII CAG'CTG
Cuts at: 1516 1876 1516
Size: 360 3731
RsrII CG'GwC_CG
Cuts at: 2286 2286
Size: 4091
SacI G_AGCT'C
Cuts at: 1105 1105
Size: 4091
SalI G'TCGA_C
Cuts at: 1128 1128
Size: 4091
SapI GCTCTTCn'nnn_
Cuts at: 2114 2324 2114
Size: 210 3881
SbfI CC_TGCA'GG
Cuts at: 1138 1138
Size: 4091
ScaI AGT'ACT
Cuts at: 3608 3608
Size: 4091
SfcI C'TryA_G
Cuts at: 33 615 981 1134 1819 2812 3003 33
Size: 582 366 153 685 993 191 1121
Fragments arranged by size:
1121 993 685 582 366 191 153
SfoI GGC'GCC
Cuts at: 174 1523 1771 174
Size: 1349 248 2494
Fragments arranged by size:
2494 1349 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4091
SmaI CCC'GGG
Cuts at: 1113 1113
Size: 4091
SmlI C'TyrA_G
Cuts at: 1246 2161 2653 2915 1246
Size: 915 492 262 2422
Fragments arranged by size:
2422 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4091
SphI G_CATG'C
Cuts at: 270 1144 1644 270
Size: 874 500 2717
Fragments arranged by size:
2717 874 500
StyI C'CwwG_G
Cuts at: 107 1225 1579 107
Size: 1118 354 2619
Fragments arranged by size:
2619 1118 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1013 1674 1807 2266 2475 1013
Size: 661 133 459 209 2629
Fragments arranged by size:
2629 661 459 209 133
TatI w'GTAC_w
Cuts at: 3606 3606
Size: 4091
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 109 141 3608 3640 109
Size: 32 3467 32 560
Fragments arranged by size:
3467 560 32 32
Tth111I GACn'n_nGTC
Cuts at: 1888 1888
Size: 4091
XbaI T'CTAG_A
Cuts at: 1122 1122
Size: 4091
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 288 1586 288
Size: 1298 2793
XmaI C'CCGG_G
Cuts at: 1111 1111
Size: 4091
XmnI GAAnn'nnTTC
Cuts at: 187 743 187
Size: 556 3535
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NruI NspI PciI PflMI PfoI PmeI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI ApaI AscI
AvrII BaeI BcgI BfrBI BglI BmtI BsaXI BsiWI
BsmBI BsrGI BssHII BstZ17I Bsu36I CspCI EcoNI FseI
FspAI HindIII HpaI NdeI NheI NotI NsiI PacI
PasI PmlI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI ZraI