Restriction map for
pF1KB6476
(Circular) (Six-base) MAPSORT of: pF1KB6476.seq Check: 9734 from: 1 to: 4178
pF1KB6476 4178 bp
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 360 360
Size: 4178
AccI GT'mk_AC
Cuts at: 1216 1216
Size: 4178
Acc65I G'GTAC_C
Cuts at: 1194 1194
Size: 4178
AclI AA'CG_TT
Cuts at: 4018 4018
Size: 4178
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 224 289 544 2003 2435 3182 3450 224
Size: 65 255 1459 432 747 268 952
Fragments arranged by size:
1459 952 747 432 268 255 65
AflIII A'CryG_T
Cuts at: 1530 2634 3611 1530
Size: 1104 977 2097
Fragments arranged by size:
2097 1104 977
AgeI A'CCGG_T
Cuts at: 2566 2566
Size: 4178
AlwNI CAG_nnn'CTG
Cuts at: 837 1483 3050 837
Size: 646 1567 1965
Fragments arranged by size:
1965 1567 646
ApaLI G'TGCA_C
Cuts at: 2948 2948
Size: 4178
ApoI r'AATT_y
Cuts at: 226 278 697 1182 3531 3787 226
Size: 52 419 485 2349 256 617
Fragments arranged by size:
2349 617 485 419 256 52
AseI AT'TA_AT
Cuts at: 20 530 1139 20
Size: 510 609 3059
Fragments arranged by size:
3059 609 510
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4178
AvaI C'yCGr_G
Cuts at: 442 1198 442
Size: 756 3422
BamHI G'GATC_C
Cuts at: 1203 1203
Size: 4178
BanI G'GyrC_C
Cuts at: 152 1194 1608 1856 1891 152
Size: 1042 414 248 35 2439
Fragments arranged by size:
2439 1042 414 248 35
BanII G_rGCy'C
Cuts at: 443 932 1192 2222 443
Size: 489 260 1030 2399
Fragments arranged by size:
2399 1030 489 260
BbeI G_GCGC'C
Cuts at: 1612 1860 1612
Size: 248 3930
BbsI GAAGACnn'nnnn_
Cuts at: 382 455 967 382
Size: 73 512 3593
Fragments arranged by size:
3593 512 73
BbvCI CC'TCA_GC
Cuts at: 506 506
Size: 4178
BciVI GTATCCnnnnn_n'
Cuts at: 379 2076 2837 4178 379
Size: 1697 761 1341 379
Fragments arranged by size:
1697 1341 761 379
BclI T'GATC_A
Cuts at: 1698 1698
Size: 4178
BfrBI ATG'CAT
Cuts at: 469 469
Size: 4178
BglII A'GATC_T
Cuts at: 1693 3730 1693
Size: 2037 2141
BlpI GC'TnA_GC
Cuts at: 329 1290 329
Size: 961 3217
Bme1580I G_kGCm'C
Cuts at: 1803 1896 2952 1803
Size: 93 1056 3029
Fragments arranged by size:
3029 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1390 1471 1806 3950 1390
Size: 81 335 2144 1618
Fragments arranged by size:
2144 1618 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2545 2545
Size: 4178
Bpu10I CC'TnA_GC
Cuts at: 506 506
Size: 4178
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1354 2233 2725 3023 1354
Size: 879 492 298 2509
Fragments arranged by size:
2509 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1012 3882 1012
Size: 2870 1308
BsaAI yAC'GTr
Cuts at: 1146 1531 1146
Size: 385 3793
BsaBI GATnn'nnATC
Cuts at: 6 1692 3729 6
Size: 1686 2037 455
Fragments arranged by size:
2037 1686 455
BsaHI Gr'CG_yC
Cuts at: 1609 1857 2559 4055 1609
Size: 248 702 1496 1732
Fragments arranged by size:
1732 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1367 1888 2566 2840 2987 3398 1367
Size: 521 678 274 147 411 2147
Fragments arranged by size:
2147 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 909 939 909
Size: 30 4148
BseYI C'CCAG_C
Cuts at: 124 408 1603 2361 2938 3434 124
Size: 284 1195 758 577 496 868
Fragments arranged by size:
1195 868 758 577 496 284
BsiEI CG_ry'CG
Cuts at: 104 1720 1766 2974 104
Size: 1616 46 1208 1308
Fragments arranged by size:
1616 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 443 932 1192 1970 2160 2952 443
Size: 489 260 778 190 792 1669
Fragments arranged by size:
1669 792 778 489 260 190
BsmI GAATG_Cn'
Cuts at: 344 344
Size: 4178
Bsp1286I G_dGCh'C
Cuts at: 443 932 1192 1803 1896 1970 2160 2222 2952
Size: 489 260 611 93 74 190 62 730
Cuts at: 2952 443
Size: 1669
Fragments arranged by size:
1669 730 611 489 260 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3398 3398
Size: 4178
BspHI T'CATG_A
Cuts at: 3354 3354
Size: 4178
BspMI ACCTGCnnnn'nnnn_
Cuts at: 360 1228 1442 1744 2125 360
Size: 868 214 302 381 2413
Fragments arranged by size:
2413 868 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2470 2524 4008 4173 50
Size: 2420 54 1484 165 55
Fragments arranged by size:
2420 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 471 2090 471
Size: 1619 2559
BsrFI r'CCGG_y
Cuts at: 2176 2357 2566 2176
Size: 181 209 3788
Fragments arranged by size:
3788 209 181
BssSI C'ACGA_G
Cuts at: 2449 2807 2449
Size: 358 3820
BstAPI GCAn_nnn'nTGC
Cuts at: 837 1555 837
Size: 718 3460
BstBI TT'CG_AA
Cuts at: 2539 2539
Size: 4178
BstYI r'GATC_y
Cuts at: 1 359 1203 1670 1693 2028 2274 3286 3372
Size: 358 844 467 23 335 246 1012 86
Cuts at: 3372 3384 3730 1
Size: 12 346 449
Fragments arranged by size:
1012 844 467 449 358 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1169 1169
Size: 4178
BtgI C'CryG_G
Cuts at: 107 983 107
Size: 876 3302
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2128 2309 2472 2128
Size: 181 163 3834
Fragments arranged by size:
3834 181 163
BtsI GCAGTG_nn'
Cuts at: 1421 1421
Size: 4178
DraI TTT'AAA
Cuts at: 584 1050 1177 584
Size: 466 127 3585
Fragments arranged by size:
3585 466 127
DrdI GACnn_nn'nnGTC
Cuts at: 676 1884 2742 676
Size: 1208 858 2112
Fragments arranged by size:
2112 1208 858
EaeI y'GGCC_r
Cuts at: 1763 1937 2328 2355 3700 1763
Size: 174 391 27 1345 2241
Fragments arranged by size:
2241 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1763 1763
Size: 4178
EarI CTCTTCn'nnn_
Cuts at: 698 2201 2411 698
Size: 1503 210 2465
Fragments arranged by size:
2465 1503 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2386 2696 2842 3988 2386
Size: 310 146 1146 2576
Fragments arranged by size:
2576 1146 310 146
EcoICRI GAG'CTC
Cuts at: 441 930 1190 441
Size: 489 260 3429
Fragments arranged by size:
3429 489 260
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 224 289 544 2003 2435 3182 3450 224
Size: 65 255 1459 432 747 268 952
Fragments arranged by size:
1459 952 747 432 268 255 65
EcoO109I rG'GnC_Cy
Cuts at: 1317 1317
Size: 4178
EcoRI G'AATT_C
Cuts at: 1182 1182
Size: 4178
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 538 2197 538
Size: 1659 2519
FspI TGC'GCA
Cuts at: 1959 1959
Size: 4178
HaeII r_GCGC'y
Cuts at: 1612 1860 2882 3861 1612
Size: 248 1022 979 1929
Fragments arranged by size:
1929 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 582 614 2020 2050 2052 2082 582
Size: 32 1406 30 2 30 2678
Fragments arranged by size:
2678 1406 32 30 30 2
HincII GTy'rAC
Cuts at: 1217 1217
Size: 4178
KasI G'GCGC_C
Cuts at: 1608 1856 1608
Size: 248 3930
KpnI G_GTAC'C
Cuts at: 1198 1198
Size: 4178
MluI A'CGCG_T
Cuts at: 3611 3611
Size: 4178
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2849 3033 2849
Size: 184 3994
MscI TGG'CCA
Cuts at: 1939 1939
Size: 4178
MslI CAynn'nnrTG
Cuts at: 982 2167 2294 982
Size: 1185 127 2866
Fragments arranged by size:
2866 1185 127
MspA1I CmG'CkG
Cuts at: 868 1289 1603 1963 2976 3221 868
Size: 421 314 360 1013 245 1825
Fragments arranged by size:
1825 1013 421 360 314 245
NaeI GCC'GGC
Cuts at: 2359 2359
Size: 4178
NarI GG'CG_CC
Cuts at: 1609 1857 1609
Size: 248 3930
NcoI C'CATG_G
Cuts at: 107 983 107
Size: 876 3302
NgoMIV G'CCGG_C
Cuts at: 2357 2357
Size: 4178
NsiI A_TGCA'T
Cuts at: 471 471
Size: 4178
NspI r_CATG'y
Cuts at: 735 1231 1731 2638 735
Size: 496 500 907 2275
Fragments arranged by size:
2275 907 500 496
PciI A'CATG_T
Cuts at: 2634 2634
Size: 4178
PmeI GTTT'AAAC
Cuts at: 1177 1177
Size: 4178
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3317 3349 3317
Size: 32 4146
PsiI TTA'TAA
Cuts at: 641 641
Size: 4178
PstI C_TGCA'G
Cuts at: 833 842 1225 1910 833
Size: 9 383 685 3101
Fragments arranged by size:
3101 685 383 9
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4178
PvuII CAG'CTG
Cuts at: 868 1603 1963 868
Size: 735 360 3083
Fragments arranged by size:
3083 735 360
RsrII CG'GwC_CG
Cuts at: 2373 2373
Size: 4178
SacI G_AGCT'C
Cuts at: 443 932 1192 443
Size: 489 260 3429
Fragments arranged by size:
3429 489 260
SalI G'TCGA_C
Cuts at: 1215 1215
Size: 4178
SapI GCTCTTCn'nnn_
Cuts at: 2201 2411 2201
Size: 210 3968
SbfI CC_TGCA'GG
Cuts at: 1225 1225
Size: 4178
ScaI AGT'ACT
Cuts at: 3695 3695
Size: 4178
SfcI C'TryA_G
Cuts at: 33 829 838 850 953 1221 1906 2899 3090
Size: 796 9 12 103 268 685 993 191
Cuts at: 3090 33
Size: 1121
Fragments arranged by size:
1121 993 796 685 268 191 103 12
9
SfoI GGC'GCC
Cuts at: 1610 1858 1610
Size: 248 3930
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4178
SmaI CCC'GGG
Cuts at: 1200 1200
Size: 4178
SmlI C'TyrA_G
Cuts at: 1333 2248 2740 3002 1333
Size: 915 492 262 2509
Fragments arranged by size:
2509 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4178
SphI G_CATG'C
Cuts at: 1231 1731 1231
Size: 500 3678
StuI AGG'CCT
Cuts at: 906 906
Size: 4178
StyI C'CwwG_G
Cuts at: 107 901 983 1032 1312 1666 107
Size: 794 82 49 280 354 2619
Fragments arranged by size:
2619 794 354 280 82 49
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1761 1894 2353 2562 1761
Size: 133 459 209 3377
Fragments arranged by size:
3377 459 209 133
TatI w'GTAC_w
Cuts at: 3693 3693
Size: 4178
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 799 831 909 941 3695 3727 799
Size: 32 78 32 2754 32 1250
Fragments arranged by size:
2754 1250 78 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1975 1975
Size: 4178
XbaI T'CTAG_A
Cuts at: 1209 1209
Size: 4178
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 498 1143 1673 498
Size: 645 530 3003
Fragments arranged by size:
3003 645 530
XmaI C'CCGG_G
Cuts at: 1198 1198
Size: 4178
XmnI GAAnn'nnTTC
Cuts at: 302 302
Size: 4178
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NsiI NspI PciI PmeI PpiI PsiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BcgI BglI BmgBI BmtI BpmI
BseRI BsgI BsiWI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI PacI
PasI PflMI PfoI PmlI PpuMI PshAI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI ZraI