Restriction map for
pF1KB6478
(Circular) (Six-base) MAPSORT of: pF1KB6478.seq Check: 9208 from: 1 to: 4178
pF1KB6478 4178 bp
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AccI GT'mk_AC
Cuts at: 1216 1216
Size: 4178
Acc65I G'GTAC_C
Cuts at: 1194 1194
Size: 4178
AclI AA'CG_TT
Cuts at: 4018 4018
Size: 4178
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 282 604 2003 2435 3182 3450 282
Size: 322 1399 432 747 268 1010
Fragments arranged by size:
1399 1010 747 432 322 268
AflIII A'CryG_T
Cuts at: 512 1530 2634 3611 512
Size: 1018 1104 977 1079
Fragments arranged by size:
1104 1079 1018 977
AgeI A'CCGG_T
Cuts at: 2566 2566
Size: 4178
AleI CACnn'nnGTG
Cuts at: 260 565 260
Size: 305 3873
AlwNI CAG_nnn'CTG
Cuts at: 1483 3050 1483
Size: 1567 2611
ApaLI G'TGCA_C
Cuts at: 2948 2948
Size: 4178
ApoI r'AATT_y
Cuts at: 1182 3531 3787 1182
Size: 2349 256 1573
Fragments arranged by size:
2349 1573 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4178
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4178
AvaI C'yCGr_G
Cuts at: 1198 1198
Size: 4178
BamHI G'GATC_C
Cuts at: 1203 1203
Size: 4178
BanI G'GyrC_C
Cuts at: 262 571 1194 1608 1856 1891 262
Size: 309 623 414 248 35 2549
Fragments arranged by size:
2549 623 414 309 248 35
BanII G_rGCy'C
Cuts at: 461 503 1004 1064 1091 1192 2222 461
Size: 42 501 60 27 101 1030 2417
Fragments arranged by size:
2417 1030 501 101 60 42 27
BbeI G_GCGC'C
Cuts at: 1612 1860 1612
Size: 248 3930
BbsI GAAGACnn'nnnn_
Cuts at: 230 230
Size: 4178
BbvCI CC'TCA_GC
Cuts at: 649 649
Size: 4178
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 835 869 835
Size: 34 4144
BciVI GTATCCnnnnn_n'
Cuts at: 2076 2837 4178 2076
Size: 761 1341 2076
Fragments arranged by size:
2076 1341 761
BclI T'GATC_A
Cuts at: 1698 1698
Size: 4178
BglI GCCn_nnn'nGGC
Cuts at: 712 806 712
Size: 94 4084
BglII A'GATC_T
Cuts at: 173 1693 3730 173
Size: 1520 2037 621
Fragments arranged by size:
2037 1520 621
BlpI GC'TnA_GC
Cuts at: 1290 1290
Size: 4178
Bme1580I G_kGCm'C
Cuts at: 574 1803 1896 2952 574
Size: 1229 93 1056 1800
Fragments arranged by size:
1800 1229 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 678 1390 1471 1806 3950 678
Size: 712 81 335 2144 906
Fragments arranged by size:
2144 906 712 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2545 2545
Size: 4178
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 217 430 940 973 1018 217
Size: 213 510 33 45 3377
Fragments arranged by size:
3377 510 213 45 33
Bpu10I CC'TnA_GC
Cuts at: 471 614 649 471
Size: 143 35 4000
Fragments arranged by size:
4000 143 35
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1354 2233 2725 3023 1354
Size: 879 492 298 2509
Fragments arranged by size:
2509 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3882 3882
Size: 4178
BsaAI yAC'GTr
Cuts at: 1531 1531
Size: 4178
BsaBI GATnn'nnATC
Cuts at: 6 1692 3729 6
Size: 1686 2037 455
Fragments arranged by size:
2037 1686 455
BsaHI Gr'CG_yC
Cuts at: 1609 1857 2559 4055 1609
Size: 248 702 1496 1732
Fragments arranged by size:
1732 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1367 1888 2566 2840 2987 3398 1367
Size: 521 678 274 147 411 2147
Fragments arranged by size:
2147 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 205 343 415 205
Size: 138 72 3968
Fragments arranged by size:
3968 138 72
BseYI C'CCAG_C
Cuts at: 744 769 1070 1168 1603 2361 2938 3434 744
Size: 25 301 98 435 758 577 496 1488
Fragments arranged by size:
1488 758 577 496 435 301 98 25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 424 588 424
Size: 164 4014
BsiEI CG_ry'CG
Cuts at: 104 690 849 1720 1766 2974 104
Size: 586 159 871 46 1208 1308
Fragments arranged by size:
1308 1208 871 586 159 46
BsiHKAI G_wGCw'C
Cuts at: 503 1192 1970 2160 2952 503
Size: 689 778 190 792 1729
Fragments arranged by size:
1729 792 778 689 190
BsiWI C'GTAC_G
Cuts at: 140 140
Size: 4178
Bsp1286I G_dGCh'C
Cuts at: 461 503 574 1004 1064 1091 1192 1803 1896
Size: 42 71 430 60 27 101 611 93
Cuts at: 1896 1970 2160 2222 2952 461
Size: 74 190 62 730 1687
Fragments arranged by size:
1687 730 611 430 190 101 93 74
71 62 60 42 27
BspEI T'CCGG_A
Cuts at: 3398 3398
Size: 4178
BspHI T'CATG_A
Cuts at: 890 902 3354 890
Size: 12 2452 1714
Fragments arranged by size:
2452 1714 12
BspMI ACCTGCnnnn'nnnn_
Cuts at: 788 836 1228 1442 1744 2125 788
Size: 48 392 214 302 381 2841
Fragments arranged by size:
2841 392 381 302 214 48
BsrBI CCG'CTC
Cuts at: 50 201 1110 2470 2524 4008 4173 50
Size: 151 909 1360 54 1484 165 55
Fragments arranged by size:
1484 1360 909 165 151 55 54
BsrDI GCAATG_nn'
Cuts at: 2090 2090
Size: 4178
BsrFI r'CCGG_y
Cuts at: 265 596 1045 2176 2357 2566 265
Size: 331 449 1131 181 209 1877
Fragments arranged by size:
1877 1131 449 331 209 181
BssHII G'CGCG_C
Cuts at: 371 371
Size: 4178
BssSI C'ACGA_G
Cuts at: 2449 2807 2449
Size: 358 3820
BstAPI GCAn_nnn'nTGC
Cuts at: 1555 1555
Size: 4178
BstBI TT'CG_AA
Cuts at: 2539 2539
Size: 4178
BstXI CCAn_nnnn'nTGG
Cuts at: 744 744
Size: 4178
BstYI r'GATC_y
Cuts at: 1 173 761 923 1203 1670 1693 2028 2274
Size: 172 588 162 280 467 23 335 246
Cuts at: 2274 3286 3372 3384 3730 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 588 467 449 346 335 280 246
172 162 86 23 12
BtgI C'CryG_G
Cuts at: 309 323 504 575 590 608 870 309
Size: 14 181 71 15 18 262 3617
Fragments arranged by size:
3617 262 181 71 18 15 14
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 737 2128 2309 2472 737
Size: 1391 181 163 2443
Fragments arranged by size:
2443 1391 181 163
BtsI GCAGTG_nn'
Cuts at: 275 1421 275
Size: 1146 3032
DraI TTT'AAA
Cuts at: 1177 1177
Size: 4178
DrdI GACnn_nn'nnGTC
Cuts at: 1884 2742 1884
Size: 858 3320
EaeI y'GGCC_r
Cuts at: 1763 1937 2328 2355 3700 1763
Size: 174 391 27 1345 2241
Fragments arranged by size:
2241 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1763 1763
Size: 4178
EarI CTCTTCn'nnn_
Cuts at: 2201 2411 2201
Size: 210 3968
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2386 2696 2842 3988 2386
Size: 310 146 1146 2576
Fragments arranged by size:
2576 1146 310 146
EcoICRI GAG'CTC
Cuts at: 501 1190 501
Size: 689 3489
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 217 282 430 604 940 973 1018 2003 2435
Size: 65 148 174 336 33 45 985 432
Cuts at: 2435 3182 3450 217
Size: 747 268 945
Fragments arranged by size:
985 945 747 432 336 268 174 148
65 45 33
EcoO109I rG'GnC_Cy
Cuts at: 304 693 1317 304
Size: 389 624 3165
Fragments arranged by size:
3165 624 389
EcoRI G'AATT_C
Cuts at: 1182 1182
Size: 4178
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2197 2197
Size: 4178
FspI TGC'GCA
Cuts at: 1959 1959
Size: 4178
HaeII r_GCGC'y
Cuts at: 400 801 964 1612 1860 2882 3861 400
Size: 401 163 648 248 1022 979 717
Fragments arranged by size:
1022 979 717 648 401 248 163
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 548 580 2020 2050 2052 2082 548
Size: 32 1440 30 2 30 2644
Fragments arranged by size:
2644 1440 32 30 30 2
HincII GTy'rAC
Cuts at: 825 882 1217 825
Size: 57 335 3786
Fragments arranged by size:
3786 335 57
KasI G'GCGC_C
Cuts at: 1608 1856 1608
Size: 248 3930
KpnI G_GTAC'C
Cuts at: 1198 1198
Size: 4178
MluI A'CGCG_T
Cuts at: 3611 3611
Size: 4178
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2849 3033 2849
Size: 184 3994
MscI TGG'CCA
Cuts at: 1939 1939
Size: 4178
MslI CAynn'nnrTG
Cuts at: 112 260 517 565 2167 2294 112
Size: 148 257 48 1602 127 1996
Fragments arranged by size:
1996 1602 257 148 127 48
MspA1I CmG'CkG
Cuts at: 408 773 786 1289 1603 1963 2976 3221 408
Size: 365 13 503 314 360 1013 245 1365
Fragments arranged by size:
1365 1013 503 365 360 314 245 13
NaeI GCC'GGC
Cuts at: 267 598 1047 2359 267
Size: 331 449 1312 2086
Fragments arranged by size:
2086 1312 449 331
NarI GG'CG_CC
Cuts at: 1609 1857 1609
Size: 248 3930
NcoI C'CATG_G
Cuts at: 323 575 870 323
Size: 252 295 3631
Fragments arranged by size:
3631 295 252
NgoMIV G'CCGG_C
Cuts at: 265 596 1045 2357 265
Size: 331 449 1312 2086
Fragments arranged by size:
2086 1312 449 331
NspI r_CATG'y
Cuts at: 516 1231 1731 2638 516
Size: 715 500 907 2056
Fragments arranged by size:
2056 907 715 500
PasI CC'CwG_GG
Cuts at: 482 482
Size: 4178
PciI A'CATG_T
Cuts at: 512 2634 512
Size: 2122 2056
PflMI CCAn_nnn'nTGG
Cuts at: 450 450
Size: 4178
PfoI T'CCnGG_A
Cuts at: 167 167
Size: 4178
PmeI GTTT'AAAC
Cuts at: 1177 1177
Size: 4178
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3317 3349 3317
Size: 32 4146
PpuMI rG'GwC_Cy
Cuts at: 304 693 304
Size: 389 3789
PstI C_TGCA'G
Cuts at: 640 761 833 1225 1910 640
Size: 121 72 392 685 2908
Fragments arranged by size:
2908 685 392 121 72
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4178
PvuII CAG'CTG
Cuts at: 408 1603 1963 408
Size: 1195 360 2623
Fragments arranged by size:
2623 1195 360
RsrII CG'GwC_CG
Cuts at: 2373 2373
Size: 4178
SacI G_AGCT'C
Cuts at: 503 1192 503
Size: 689 3489
SalI G'TCGA_C
Cuts at: 1215 1215
Size: 4178
SanDI GG'GwC_CC
Cuts at: 304 304
Size: 4178
SapI GCTCTTCn'nnn_
Cuts at: 2201 2411 2201
Size: 210 3968
SbfI CC_TGCA'GG
Cuts at: 640 1225 640
Size: 585 3593
ScaI AGT'ACT
Cuts at: 3695 3695
Size: 4178
SfcI C'TryA_G
Cuts at: 33 636 757 829 1221 1906 2899 3090 33
Size: 603 121 72 392 685 993 191 1121
Fragments arranged by size:
1121 993 685 603 392 191 121 72
SfoI GGC'GCC
Cuts at: 1610 1858 1610
Size: 248 3930
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4178
SmaI CCC'GGG
Cuts at: 1200 1200
Size: 4178
SmlI C'TyrA_G
Cuts at: 1333 2248 2740 3002 1333
Size: 915 492 262 2509
Fragments arranged by size:
2509 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4178
SphI G_CATG'C
Cuts at: 1231 1731 1231
Size: 500 3678
StyI C'CwwG_G
Cuts at: 323 575 870 1004 1157 1312 1666 323
Size: 252 295 134 153 155 354 2835
Fragments arranged by size:
2835 354 295 252 155 153 134
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1761 1894 2353 2562 1761
Size: 133 459 209 3377
Fragments arranged by size:
3377 459 209 133
TatI w'GTAC_w
Cuts at: 3693 3693
Size: 4178
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3695 3727 3695
Size: 32 4146
Tth111I GACn'n_nGTC
Cuts at: 562 886 1975 562
Size: 324 1089 2765
Fragments arranged by size:
2765 1089 324
XbaI T'CTAG_A
Cuts at: 1209 1209
Size: 4178
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 489 993 1673 489
Size: 504 680 2994
Fragments arranged by size:
2994 680 504
XmaI C'CCGG_G
Cuts at: 1198 1198
Size: 4178
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsiWI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BfrBI BmgBI BmtI BsaXI BsmI
BsmBI BsrGI BstEII BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PmlI PshAI PsiI
PspOMI PspXI PsrI SacII SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI