Restriction map for
pF1KB6483
(Circular) (Six-base) MAPSORT of: pF1KB6483.seq Check: 4920 from: 1 to: 4097
pF1KB6483 4097 bp
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 968 968
Size: 4097
AccI GT'mk_AC
Cuts at: 1135 1135
Size: 4097
Acc65I G'GTAC_C
Cuts at: 1113 1113
Size: 4097
AclI AA'CG_TT
Cuts at: 3937 3937
Size: 4097
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1922 2354 3101 3369 1922
Size: 432 747 268 2650
Fragments arranged by size:
2650 747 432 268
AflIII A'CryG_T
Cuts at: 476 1028 1449 2553 3530 476
Size: 552 421 1104 977 1043
Fragments arranged by size:
1104 1043 977 552 421
AgeI A'CCGG_T
Cuts at: 2485 2485
Size: 4097
AlwNI CAG_nnn'CTG
Cuts at: 942 1402 2969 942
Size: 460 1567 2070
Fragments arranged by size:
2070 1567 460
ApaI G_GGCC'C
Cuts at: 162 162
Size: 4097
ApaLI G'TGCA_C
Cuts at: 208 2867 208
Size: 2659 1438
ApoI r'AATT_y
Cuts at: 307 559 1101 3450 3706 307
Size: 252 542 2349 256 698
Fragments arranged by size:
2349 698 542 256 252
AseI AT'TA_AT
Cuts at: 20 20
Size: 4097
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4097
AvaI C'yCGr_G
Cuts at: 605 659 688 1117 605
Size: 54 29 429 3585
Fragments arranged by size:
3585 429 54 29
BamHI G'GATC_C
Cuts at: 1122 1122
Size: 4097
BanI G'GyrC_C
Cuts at: 884 1076 1113 1527 1775 1810 884
Size: 192 37 414 248 35 3171
Fragments arranged by size:
3171 414 248 192 37 35
BanII G_rGCy'C
Cuts at: 162 1111 2141 162
Size: 949 1030 2118
Fragments arranged by size:
2118 1030 949
BbeI G_GCGC'C
Cuts at: 1531 1779 1531
Size: 248 3849
BciVI GTATCCnnnnn_n'
Cuts at: 1995 2756 4097 1995
Size: 761 1341 1995
Fragments arranged by size:
1995 1341 761
BclI T'GATC_A
Cuts at: 1617 1617
Size: 4097
BglI GCCn_nnn'nGGC
Cuts at: 194 194
Size: 4097
BglII A'GATC_T
Cuts at: 1612 3649 1612
Size: 2037 2060
BlpI GC'TnA_GC
Cuts at: 997 1209 997
Size: 212 3885
Bme1580I G_kGCm'C
Cuts at: 162 212 887 1722 1815 2871 162
Size: 50 675 835 93 1056 1388
Fragments arranged by size:
1388 1056 835 675 93 50
BmgBI CAC'GTC
Cuts at: 570 570
Size: 4097
BmrI ACTGGGnnnn_n'
Cuts at: 921 1309 1390 1725 3869 921
Size: 388 81 335 2144 1149
Fragments arranged by size:
2144 1149 388 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 251 2464 251
Size: 2213 1884
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 754 754
Size: 4097
Bpu10I CC'TnA_GC
Cuts at: 226 226
Size: 4097
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1273 2152 2644 2942 1273
Size: 879 492 298 2428
Fragments arranged by size:
2428 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 577 3801 577
Size: 3224 873
BsaAI yAC'GTr
Cuts at: 479 1450 479
Size: 971 3126
BsaBI GATnn'nnATC
Cuts at: 6 1611 3648 6
Size: 1605 2037 455
Fragments arranged by size:
2037 1605 455
BsaHI Gr'CG_yC
Cuts at: 248 1528 1776 2478 3974 248
Size: 1280 248 702 1496 371
Fragments arranged by size:
1496 1280 702 371 248
BsaWI w'CCGG_w
Cuts at: 1286 1807 2485 2759 2906 3317 1286
Size: 521 678 274 147 411 2066
Fragments arranged by size:
2066 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 811 841 811
Size: 30 4067
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 529 679 706 790 829 832 529
Size: 150 27 84 39 3 3794
Fragments arranged by size:
3794 150 84 39 27 3
BseYI C'CCAG_C
Cuts at: 392 1522 2280 2857 3353 392
Size: 1130 758 577 496 1136
Fragments arranged by size:
1136 1130 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 667 853 949 667
Size: 186 96 3815
Fragments arranged by size:
3815 186 96
BsiEI CG_ry'CG
Cuts at: 104 1639 1685 2893 104
Size: 1535 46 1208 1308
Fragments arranged by size:
1535 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 212 1111 1889 2079 2871 212
Size: 899 778 190 792 1438
Fragments arranged by size:
1438 899 792 778 190
BsiWI C'GTAC_G
Cuts at: 479 479
Size: 4097
Bsp1286I G_dGCh'C
Cuts at: 162 212 887 1111 1722 1815 1889 2079 2141
Size: 50 675 224 611 93 74 190 62
Cuts at: 2141 2871 162
Size: 730 1388
Fragments arranged by size:
1388 730 675 611 224 190 93 74
62 50
BspEI T'CCGG_A
Cuts at: 3317 3317
Size: 4097
BspHI T'CATG_A
Cuts at: 564 3273 564
Size: 2709 1388
BspMI ACCTGCnnnn'nnnn_
Cuts at: 968 1147 1361 1663 2044 968
Size: 179 214 302 381 3021
Fragments arranged by size:
3021 381 302 214 179
BsrBI CCG'CTC
Cuts at: 50 615 2389 2443 3927 4092 50
Size: 565 1774 54 1484 165 55
Fragments arranged by size:
1774 1484 565 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2009 2009
Size: 4097
BsrFI r'CCGG_y
Cuts at: 822 2095 2276 2485 822
Size: 1273 181 209 2434
Fragments arranged by size:
2434 1273 209 181
BssSI C'ACGA_G
Cuts at: 967 2368 2726 967
Size: 1401 358 2338
Fragments arranged by size:
2338 1401 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1474 1474
Size: 4097
BstBI TT'CG_AA
Cuts at: 2458 2458
Size: 4097
BstYI r'GATC_y
Cuts at: 1 789 867 1122 1589 1612 1947 2193 3205
Size: 788 78 255 467 23 335 246 1012
Cuts at: 3205 3291 3303 3649 1
Size: 86 12 346 449
Fragments arranged by size:
1012 788 467 449 346 335 255 246
86 78 23 12
BtgI C'CryG_G
Cuts at: 252 252
Size: 4097
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 768 2047 2228 2391 768
Size: 1279 181 163 2474
Fragments arranged by size:
2474 1279 181 163
BtsI GCAGTG_nn'
Cuts at: 1340 1340
Size: 4097
DraI TTT'AAA
Cuts at: 1096 1096
Size: 4097
DraIII CAC_nnn'GTG
Cuts at: 915 915
Size: 4097
DrdI GACnn_nn'nnGTC
Cuts at: 1803 2661 1803
Size: 858 3239
EaeI y'GGCC_r
Cuts at: 195 299 404 537 629 653 1682 1856 2247
Size: 104 105 133 92 24 1029 174 391
Cuts at: 2247 2274 3619 195
Size: 27 1345 673
Fragments arranged by size:
1345 1029 673 391 174 133 105 104
92 27 24
EagI C'GGCC_G
Cuts at: 1682 1682
Size: 4097
EarI CTCTTCn'nnn_
Cuts at: 2120 2330 2120
Size: 210 3887
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 366 2305 2615 2761 3907 366
Size: 1939 310 146 1146 556
Fragments arranged by size:
1939 1146 556 310 146
EcoICRI GAG'CTC
Cuts at: 1109 1109
Size: 4097
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 754 1922 2354 3101 3369 754
Size: 1168 432 747 268 1482
Fragments arranged by size:
1482 1168 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 158 187 738 1065 1236 158
Size: 29 551 327 171 3019
Fragments arranged by size:
3019 551 327 171 29
EcoRI G'AATT_C
Cuts at: 307 1101 307
Size: 794 3303
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2116 2116
Size: 4097
FspI TGC'GCA
Cuts at: 523 1878 523
Size: 1355 2742
HaeII r_GCGC'y
Cuts at: 1531 1779 2801 3780 1531
Size: 248 1022 979 1848
Fragments arranged by size:
1848 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 588 620 631 642 663 674 781 813 1939
Size: 32 11 11 21 11 107 32 1126
Cuts at: 1939 1969 1971 2001 588
Size: 30 2 30 2684
Fragments arranged by size:
2684 1126 107 32 32 30 30 21
11 11 11 2
HincII GTy'rAC
Cuts at: 1136 1136
Size: 4097
KasI G'GCGC_C
Cuts at: 1527 1775 1527
Size: 248 3849
KpnI G_GTAC'C
Cuts at: 1117 1117
Size: 4097
MluI A'CGCG_T
Cuts at: 3530 3530
Size: 4097
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2768 2952 2768
Size: 184 3913
MscI TGG'CCA
Cuts at: 197 301 539 655 1858 197
Size: 104 238 116 1203 2436
Fragments arranged by size:
2436 1203 238 116 104
MslI CAynn'nnrTG
Cuts at: 890 1033 1082 2086 2213 890
Size: 143 49 1004 127 2774
Fragments arranged by size:
2774 1004 143 127 49
MspA1I CmG'CkG
Cuts at: 441 1208 1522 1882 2895 3140 441
Size: 767 314 360 1013 245 1398
Fragments arranged by size:
1398 1013 767 360 314 245
NaeI GCC'GGC
Cuts at: 824 2278 824
Size: 1454 2643
NarI GG'CG_CC
Cuts at: 1528 1776 1528
Size: 248 3849
NgoMIV G'CCGG_C
Cuts at: 822 2276 822
Size: 1454 2643
NspI r_CATG'y
Cuts at: 708 1032 1150 1650 2557 708
Size: 324 118 500 907 2248
Fragments arranged by size:
2248 907 500 324 118
PasI CC'CwG_GG
Cuts at: 162 162
Size: 4097
PciI A'CATG_T
Cuts at: 1028 2553 1028
Size: 1525 2572
PflMI CCAn_nnn'nTGG
Cuts at: 113 942 113
Size: 829 3268
PfoI T'CCnGG_A
Cuts at: 854 863 854
Size: 9 4088
PmeI GTTT'AAAC
Cuts at: 1096 1096
Size: 4097
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3236 3268 3236
Size: 32 4065
PpuMI rG'GwC_Cy
Cuts at: 738 1065 738
Size: 327 3770
PspOMI G'GGCC_C
Cuts at: 158 158
Size: 4097
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 250 282 250
Size: 32 4065
PstI C_TGCA'G
Cuts at: 1144 1829 1144
Size: 685 3412
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4097
PvuII CAG'CTG
Cuts at: 1522 1882 1522
Size: 360 3737
RsrII CG'GwC_CG
Cuts at: 489 2292 489
Size: 1803 2294
SacI G_AGCT'C
Cuts at: 1111 1111
Size: 4097
SalI G'TCGA_C
Cuts at: 1134 1134
Size: 4097
SapI GCTCTTCn'nnn_
Cuts at: 2120 2330 2120
Size: 210 3887
SbfI CC_TGCA'GG
Cuts at: 1144 1144
Size: 4097
ScaI AGT'ACT
Cuts at: 3614 3614
Size: 4097
SfcI C'TryA_G
Cuts at: 33 1140 1825 2818 3009 33
Size: 1107 685 993 191 1121
Fragments arranged by size:
1121 1107 993 685 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 194 194
Size: 4097
SfoI GGC'GCC
Cuts at: 1529 1777 1529
Size: 248 3849
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4097
SmaI CCC'GGG
Cuts at: 1119 1119
Size: 4097
SmlI C'TyrA_G
Cuts at: 659 1252 2167 2659 2921 659
Size: 593 915 492 262 1835
Fragments arranged by size:
1835 915 593 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4097
SphI G_CATG'C
Cuts at: 1150 1650 1150
Size: 500 3597
StyI C'CwwG_G
Cuts at: 302 1231 1585 302
Size: 929 354 2814
Fragments arranged by size:
2814 929 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 384 819 903 1680 1813 2272 2481 384
Size: 435 84 777 133 459 209 2000
Fragments arranged by size:
2000 777 459 435 209 133 84
TatI w'GTAC_w
Cuts at: 779 794 3612 779
Size: 15 2818 1264
Fragments arranged by size:
2818 1264 15
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 281 313 811 843 3614 3646 281
Size: 32 498 32 2771 32 732
Fragments arranged by size:
2771 732 498 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1894 1894
Size: 4097
XbaI T'CTAG_A
Cuts at: 1128 1128
Size: 4097
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1592 1592
Size: 4097
XhoI C'TCGA_G
Cuts at: 659 659
Size: 4097
XmaI C'CCGG_G
Cuts at: 1117 1117
Size: 4097
XmnI GAAnn'nnTTC
Cuts at: 560 560
Size: 4097
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsiWI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PspOMI PsrI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfiI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BmtI BsmI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
CspCI EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PmlI
PshAI PsiI PspXI SacII SanDI SexAI SgrAI SnaBI
SrfI SspI StuI SwaI ZraI