Restriction map for
pF1KB6484
(Circular) (Six-base) MAPSORT of: pF1KB6484.seq Check: 3614 from: 1 to: 4181
pF1KB6484 4181 bp
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AccI GT'mk_AC
Cuts at: 577 1071 1219 577
Size: 494 148 3539
Fragments arranged by size:
3539 494 148
Acc65I G'GTAC_C
Cuts at: 1197 1197
Size: 4181
AclI AA'CG_TT
Cuts at: 4021 4021
Size: 4181
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2006 2438 3185 3453 2006
Size: 432 747 268 2734
Fragments arranged by size:
2734 747 432 268
AflIII A'CryG_T
Cuts at: 689 1533 2637 3614 689
Size: 844 1104 977 1256
Fragments arranged by size:
1256 1104 977 844
AgeI A'CCGG_T
Cuts at: 2569 2569
Size: 4181
AlwNI CAG_nnn'CTG
Cuts at: 244 1486 3053 244
Size: 1242 1567 1372
Fragments arranged by size:
1567 1372 1242
ApaLI G'TGCA_C
Cuts at: 2951 2951
Size: 4181
ApoI r'AATT_y
Cuts at: 1120 1185 3534 3790 1120
Size: 65 2349 256 1511
Fragments arranged by size:
2349 1511 256 65
AseI AT'TA_AT
Cuts at: 20 20
Size: 4181
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4181
AvaI C'yCGr_G
Cuts at: 1201 1201
Size: 4181
BamHI G'GATC_C
Cuts at: 1206 1206
Size: 4181
BanI G'GyrC_C
Cuts at: 1197 1611 1859 1894 1197
Size: 414 248 35 3484
Fragments arranged by size:
3484 414 248 35
BanII G_rGCy'C
Cuts at: 1195 2225 1195
Size: 1030 3151
BbeI G_GCGC'C
Cuts at: 1615 1863 1615
Size: 248 3933
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1155 1189 1155
Size: 34 4147
BciVI GTATCCnnnnn_n'
Cuts at: 2079 2840 4181 2079
Size: 761 1341 2079
Fragments arranged by size:
2079 1341 761
BclI T'GATC_A
Cuts at: 1701 1701
Size: 4181
BglII A'GATC_T
Cuts at: 1696 3733 1696
Size: 2037 2144
BlpI GC'TnA_GC
Cuts at: 539 1293 539
Size: 754 3427
Bme1580I G_kGCm'C
Cuts at: 1806 1899 2955 1806
Size: 93 1056 3032
Fragments arranged by size:
3032 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1393 1474 1809 3953 1393
Size: 81 335 2144 1621
Fragments arranged by size:
2144 1621 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2548 2548
Size: 4181
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1357 2236 2728 3026 1357
Size: 879 492 298 2512
Fragments arranged by size:
2512 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3885 3885
Size: 4181
BsaAI yAC'GTr
Cuts at: 277 690 1534 277
Size: 413 844 2924
Fragments arranged by size:
2924 844 413
BsaBI GATnn'nnATC
Cuts at: 6 1695 3732 6
Size: 1689 2037 455
Fragments arranged by size:
2037 1689 455
BsaHI Gr'CG_yC
Cuts at: 1612 1860 2562 4058 1612
Size: 248 702 1496 1735
Fragments arranged by size:
1735 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1370 1891 2569 2843 2990 3401 1370
Size: 521 678 274 147 411 2150
Fragments arranged by size:
2150 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 902 932 902
Size: 30 4151
BseYI C'CCAG_C
Cuts at: 179 667 1077 1606 2364 2941 3437 179
Size: 488 410 529 758 577 496 923
Fragments arranged by size:
923 758 577 529 496 488 410
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 217 217
Size: 4181
BsiEI CG_ry'CG
Cuts at: 104 1723 1769 2977 104
Size: 1619 46 1208 1308
Fragments arranged by size:
1619 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1195 1973 2163 2955 1195
Size: 778 190 792 2421
Fragments arranged by size:
2421 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 1195 1806 1899 1973 2163 2225 2955 1195
Size: 611 93 74 190 62 730 2421
Fragments arranged by size:
2421 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3401 3401
Size: 4181
BspHI T'CATG_A
Cuts at: 3357 3357
Size: 4181
BspMI ACCTGCnnnn'nnnn_
Cuts at: 589 1231 1445 1747 2128 589
Size: 642 214 302 381 2642
Fragments arranged by size:
2642 642 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2473 2527 4011 4176 50
Size: 2423 54 1484 165 55
Fragments arranged by size:
2423 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 365 2093 365
Size: 1728 2453
BsrFI r'CCGG_y
Cuts at: 2179 2360 2569 2179
Size: 181 209 3791
Fragments arranged by size:
3791 209 181
BssSI C'ACGA_G
Cuts at: 2452 2810 2452
Size: 358 3823
BstAPI GCAn_nnn'nTGC
Cuts at: 549 1558 549
Size: 1009 3172
BstBI TT'CG_AA
Cuts at: 2542 2542
Size: 4181
BstXI CCAn_nnnn'nTGG
Cuts at: 1125 1125
Size: 4181
BstYI r'GATC_y
Cuts at: 1 1206 1673 1696 2031 2277 3289 3375 3387
Size: 1205 467 23 335 246 1012 86 12
Cuts at: 3387 3733 1
Size: 346 449
Fragments arranged by size:
1205 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 560 560
Size: 4181
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2131 2312 2475 2131
Size: 181 163 3837
Fragments arranged by size:
3837 181 163
BtsI GCAGTG_nn'
Cuts at: 205 1424 205
Size: 1219 2962
DraI TTT'AAA
Cuts at: 495 1180 495
Size: 685 3496
DrdI GACnn_nn'nnGTC
Cuts at: 424 1887 2745 424
Size: 1463 858 1860
Fragments arranged by size:
1860 1463 858
EaeI y'GGCC_r
Cuts at: 1115 1766 1940 2331 2358 3703 1115
Size: 651 174 391 27 1345 1593
Fragments arranged by size:
1593 1345 651 391 174 27
EagI C'GGCC_G
Cuts at: 1766 1766
Size: 4181
EarI CTCTTCn'nnn_
Cuts at: 2204 2414 2204
Size: 210 3971
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2389 2699 2845 3991 2389
Size: 310 146 1146 2579
Fragments arranged by size:
2579 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1193 1193
Size: 4181
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 2006 2438 3185 3453 2006
Size: 432 747 268 2734
Fragments arranged by size:
2734 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 987 1320 987
Size: 333 3848
EcoRI G'AATT_C
Cuts at: 1185 1185
Size: 4181
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 805 2200 805
Size: 1395 2786
FspI TGC'GCA
Cuts at: 1962 1962
Size: 4181
HaeII r_GCGC'y
Cuts at: 1615 1863 2885 3864 1615
Size: 248 1022 979 1932
Fragments arranged by size:
1932 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2023 2053 2055 2085 2023
Size: 30 2 30 4119
Fragments arranged by size:
4119 30 30 2
HincII GTy'rAC
Cuts at: 519 1220 519
Size: 701 3480
KasI G'GCGC_C
Cuts at: 1611 1859 1611
Size: 248 3933
KpnI G_GTAC'C
Cuts at: 1201 1201
Size: 4181
MluI A'CGCG_T
Cuts at: 3614 3614
Size: 4181
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 273 2852 3036 273
Size: 2579 184 1418
Fragments arranged by size:
2579 1418 184
MscI TGG'CCA
Cuts at: 1117 1942 1117
Size: 825 3356
MslI CAynn'nnrTG
Cuts at: 1013 2170 2297 1013
Size: 1157 127 2897
Fragments arranged by size:
2897 1157 127
MspA1I CmG'CkG
Cuts at: 183 1077 1292 1606 1966 2979 3224 183
Size: 894 215 314 360 1013 245 1140
Fragments arranged by size:
1140 1013 894 360 314 245 215
NaeI GCC'GGC
Cuts at: 2362 2362
Size: 4181
NarI GG'CG_CC
Cuts at: 1612 1860 1612
Size: 248 3933
NcoI C'CATG_G
Cuts at: 560 560
Size: 4181
NgoMIV G'CCGG_C
Cuts at: 2360 2360
Size: 4181
NspI r_CATG'y
Cuts at: 547 1234 1734 2641 547
Size: 687 500 907 2087
Fragments arranged by size:
2087 907 687 500
PciI A'CATG_T
Cuts at: 2637 2637
Size: 4181
PmeI GTTT'AAAC
Cuts at: 1180 1180
Size: 4181
PmlI CAC'GTG
Cuts at: 690 690
Size: 4181
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3320 3352 3320
Size: 32 4149
PsiI TTA'TAA
Cuts at: 1136 1136
Size: 4181
PstI C_TGCA'G
Cuts at: 586 808 834 1228 1913 586
Size: 222 26 394 685 2854
Fragments arranged by size:
2854 685 394 222 26
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4181
PvuII CAG'CTG
Cuts at: 183 1077 1606 1966 183
Size: 894 529 360 2398
Fragments arranged by size:
2398 894 529 360
RsrII CG'GwC_CG
Cuts at: 2376 2376
Size: 4181
SacI G_AGCT'C
Cuts at: 1195 1195
Size: 4181
SalI G'TCGA_C
Cuts at: 1218 1218
Size: 4181
SapI GCTCTTCn'nnn_
Cuts at: 2204 2414 2204
Size: 210 3971
SbfI CC_TGCA'GG
Cuts at: 1228 1228
Size: 4181
ScaI AGT'ACT
Cuts at: 3698 3698
Size: 4181
SexAI A'CCwGG_T
Cuts at: 191 191
Size: 4181
SfcI C'TryA_G
Cuts at: 33 582 804 830 1072 1224 1909 2902 3093
Size: 549 222 26 242 152 685 993 191
Cuts at: 3093 33
Size: 1121
Fragments arranged by size:
1121 993 685 549 242 222 191 152
26
SfoI GGC'GCC
Cuts at: 1613 1861 1613
Size: 248 3933
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4181
SmaI CCC'GGG
Cuts at: 1203 1203
Size: 4181
SmlI C'TyrA_G
Cuts at: 1336 2251 2743 3005 1336
Size: 915 492 262 2512
Fragments arranged by size:
2512 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4181
SphI G_CATG'C
Cuts at: 547 1234 1734 547
Size: 687 500 2994
Fragments arranged by size:
2994 687 500
StyI C'CwwG_G
Cuts at: 143 560 1315 1669 143
Size: 417 755 354 2655
Fragments arranged by size:
2655 755 417 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 108 434 1764 1897 2356 2565 108
Size: 326 1330 133 459 209 1724
Fragments arranged by size:
1724 1330 459 326 209 133
TatI w'GTAC_w
Cuts at: 3696 3696
Size: 4181
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 902 934 3698 3730 902
Size: 32 2764 32 1353
Fragments arranged by size:
2764 1353 32 32
Tth111I GACn'n_nGTC
Cuts at: 1978 1978
Size: 4181
XbaI T'CTAG_A
Cuts at: 1212 1212
Size: 4181
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1098 1676 1098
Size: 578 3603
XmaI C'CCGG_G
Cuts at: 1201 1201
Size: 4181
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BcgI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PmeI PmlI
PpiI PsiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BfrBI BglI
BmgBI BmtI BpmI Bpu10I BseRI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PpuMI PshAI PspOMI PspXI PsrI SacII SanDI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI