Restriction map for
pF1KB6485
(Circular) (Six-base) MAPSORT of: pF1KB6485.seq Check: 424 from: 1 to: 4097
pF1KB6485 4097 bp
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 672 672
Size: 4097
AccI GT'mk_AC
Cuts at: 1034 1135 1034
Size: 101 3996
Acc65I G'GTAC_C
Cuts at: 1113 1113
Size: 4097
AclI AA'CG_TT
Cuts at: 3937 3937
Size: 4097
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 352 541 680 829 1922 2354 3101 3369 352
Size: 189 139 149 1093 432 747 268 1080
Fragments arranged by size:
1093 1080 747 432 268 189 149 139
AflIII A'CryG_T
Cuts at: 1449 2553 3530 1449
Size: 1104 977 2016
Fragments arranged by size:
2016 1104 977
AgeI A'CCGG_T
Cuts at: 2485 2485
Size: 4097
AleI CACnn'nnGTG
Cuts at: 736 736
Size: 4097
AlwNI CAG_nnn'CTG
Cuts at: 642 703 1402 2969 642
Size: 61 699 1567 1770
Fragments arranged by size:
1770 1567 699 61
ApaLI G'TGCA_C
Cuts at: 2867 2867
Size: 4097
ApoI r'AATT_y
Cuts at: 1101 3450 3706 1101
Size: 2349 256 1492
Fragments arranged by size:
2349 1492 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4097
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4097
AvaI C'yCGr_G
Cuts at: 800 821 863 1117 800
Size: 21 42 254 3780
Fragments arranged by size:
3780 254 42 21
BamHI G'GATC_C
Cuts at: 605 1122 605
Size: 517 3580
BanI G'GyrC_C
Cuts at: 1113 1527 1775 1810 1113
Size: 414 248 35 3400
Fragments arranged by size:
3400 414 248 35
BanII G_rGCy'C
Cuts at: 662 1111 2141 662
Size: 449 1030 2618
Fragments arranged by size:
2618 1030 449
BbeI G_GCGC'C
Cuts at: 1531 1779 1531
Size: 248 3849
BbsI GAAGACnn'nnnn_
Cuts at: 340 817 340
Size: 477 3620
BciVI GTATCCnnnnn_n'
Cuts at: 1995 2756 4097 1995
Size: 761 1341 1995
Fragments arranged by size:
1995 1341 761
BclI T'GATC_A
Cuts at: 1083 1617 1083
Size: 534 3563
BfrBI ATG'CAT
Cuts at: 622 622
Size: 4097
BglII A'GATC_T
Cuts at: 1612 3649 1612
Size: 2037 2060
BlpI GC'TnA_GC
Cuts at: 1209 1209
Size: 4097
Bme1580I G_kGCm'C
Cuts at: 136 923 1722 1815 2871 136
Size: 787 799 93 1056 1362
Fragments arranged by size:
1362 1056 799 787 93
BmrI ACTGGGnnnn_n'
Cuts at: 1309 1390 1725 3869 1309
Size: 81 335 2144 1537
Fragments arranged by size:
2144 1537 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2464 2464
Size: 4097
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 425 532 930 425
Size: 107 398 3592
Fragments arranged by size:
3592 398 107
Bpu10I CC'TnA_GC
Cuts at: 118 118
Size: 4097
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1273 2152 2644 2942 1273
Size: 879 492 298 2428
Fragments arranged by size:
2428 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 400 3801 400
Size: 3401 696
BsaAI yAC'GTr
Cuts at: 1450 1450
Size: 4097
BsaBI GATnn'nnATC
Cuts at: 6 1611 3648 6
Size: 1605 2037 455
Fragments arranged by size:
2037 1605 455
BsaHI Gr'CG_yC
Cuts at: 1528 1776 2478 3974 1528
Size: 248 702 1496 1651
Fragments arranged by size:
1651 1496 702 248
BsaWI w'CCGG_w
Cuts at: 599 1286 1807 2485 2759 2906 3317 599
Size: 687 521 678 274 147 411 1379
Fragments arranged by size:
1379 687 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 163 535 592 163
Size: 372 57 3668
Fragments arranged by size:
3668 372 57
BseYI C'CCAG_C
Cuts at: 677 922 1522 2280 2857 3353 677
Size: 245 600 758 577 496 1421
Fragments arranged by size:
1421 758 600 577 496 245
BsiEI CG_ry'CG
Cuts at: 104 975 1639 1685 2893 104
Size: 871 664 46 1208 1308
Fragments arranged by size:
1308 1208 871 664 46
BsiHKAI G_wGCw'C
Cuts at: 119 662 1111 1889 2079 2871 119
Size: 543 449 778 190 792 1345
Fragments arranged by size:
1345 792 778 543 449 190
Bsp1286I G_dGCh'C
Cuts at: 119 136 662 923 1111 1722 1815 1889 2079
Size: 17 526 261 188 611 93 74 190
Cuts at: 2079 2141 2871 119
Size: 62 730 1345
Fragments arranged by size:
1345 730 611 526 261 190 188 93
74 62 17
BspEI T'CCGG_A
Cuts at: 599 3317 599
Size: 2718 1379
BspHI T'CATG_A
Cuts at: 3273 3273
Size: 4097
BspMI ACCTGCnnnn'nnnn_
Cuts at: 672 900 1147 1361 1663 2044 672
Size: 228 247 214 302 381 2725
Fragments arranged by size:
2725 381 302 247 228 214
BsrBI CCG'CTC
Cuts at: 50 2389 2443 3927 4092 50
Size: 2339 54 1484 165 55
Fragments arranged by size:
2339 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2009 2009
Size: 4097
BsrFI r'CCGG_y
Cuts at: 974 2095 2276 2485 974
Size: 1121 181 209 2586
Fragments arranged by size:
2586 1121 209 181
BssSI C'ACGA_G
Cuts at: 2368 2726 2368
Size: 358 3739
BstAPI GCAn_nnn'nTGC
Cuts at: 1474 1474
Size: 4097
BstBI TT'CG_AA
Cuts at: 2458 2458
Size: 4097
BstEII G'GTnAC_C
Cuts at: 846 846
Size: 4097
BstYI r'GATC_y
Cuts at: 1 605 1122 1589 1612 1947 2193 3205 3291
Size: 604 517 467 23 335 246 1012 86
Cuts at: 3291 3303 3649 1
Size: 12 346 449
Fragments arranged by size:
1012 604 517 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 265 265
Size: 4097
BtgI C'CryG_G
Cuts at: 107 483 107
Size: 376 3721
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2047 2228 2391 2047
Size: 181 163 3753
Fragments arranged by size:
3753 181 163
BtsI GCAGTG_nn'
Cuts at: 341 731 845 1340 341
Size: 390 114 495 3098
Fragments arranged by size:
3098 495 390 114
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 345 380 345
Size: 35 4062
DraI TTT'AAA
Cuts at: 1096 1096
Size: 4097
DrdI GACnn_nn'nnGTC
Cuts at: 1803 2661 1803
Size: 858 3239
EaeI y'GGCC_r
Cuts at: 972 992 1682 1856 2247 2274 3619 972
Size: 20 690 174 391 27 1345 1450
Fragments arranged by size:
1450 1345 690 391 174 27 20
EagI C'GGCC_G
Cuts at: 972 1682 972
Size: 710 3387
EarI CTCTTCn'nnn_
Cuts at: 212 2120 2330 212
Size: 1908 210 1979
Fragments arranged by size:
1979 1908 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2305 2615 2761 3907 2305
Size: 310 146 1146 2495
Fragments arranged by size:
2495 1146 310 146
EcoICRI GAG'CTC
Cuts at: 660 1109 660
Size: 449 3648
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 352 425 532 541 680 829 930 1922 2354
Size: 73 107 9 139 149 101 992 432
Cuts at: 2354 3101 3369 352
Size: 747 268 1080
Fragments arranged by size:
1080 992 747 432 268 149 139 107
101 73 9
EcoNI CCTnn'n_nnAGG
Cuts at: 145 217 145
Size: 72 4025
EcoO109I rG'GnC_Cy
Cuts at: 252 825 1043 1052 1236 252
Size: 573 218 9 184 3113
Fragments arranged by size:
3113 573 218 184 9
EcoRI G'AATT_C
Cuts at: 1101 1101
Size: 4097
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2116 2116
Size: 4097
FspI TGC'GCA
Cuts at: 1878 1878
Size: 4097
HaeII r_GCGC'y
Cuts at: 314 1531 1779 2801 3780 314
Size: 1217 248 1022 979 631
Fragments arranged by size:
1217 1022 979 631 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1939 1969 1971 2001 1939
Size: 30 2 30 4035
Fragments arranged by size:
4035 30 30 2
HincII GTy'rAC
Cuts at: 1136 1136
Size: 4097
KasI G'GCGC_C
Cuts at: 1527 1775 1527
Size: 248 3849
KpnI G_GTAC'C
Cuts at: 1117 1117
Size: 4097
MluI A'CGCG_T
Cuts at: 3530 3530
Size: 4097
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 105 2768 2952 105
Size: 2663 184 1250
Fragments arranged by size:
2663 1250 184
MscI TGG'CCA
Cuts at: 994 1858 994
Size: 864 3233
MslI CAynn'nnrTG
Cuts at: 112 736 838 2086 2213 112
Size: 624 102 1248 127 1996
Fragments arranged by size:
1996 1248 624 127 102
MspA1I CmG'CkG
Cuts at: 552 795 878 1208 1522 1882 2895 3140 552
Size: 243 83 330 314 360 1013 245 1509
Fragments arranged by size:
1509 1013 360 330 314 245 243 83
NaeI GCC'GGC
Cuts at: 976 2278 976
Size: 1302 2795
NarI GG'CG_CC
Cuts at: 1528 1776 1528
Size: 248 3849
NcoI C'CATG_G
Cuts at: 107 483 107
Size: 376 3721
NgoMIV G'CCGG_C
Cuts at: 974 2276 974
Size: 1302 2795
NsiI A_TGCA'T
Cuts at: 624 624
Size: 4097
NspI r_CATG'y
Cuts at: 622 1150 1650 2557 622
Size: 528 500 907 2162
Fragments arranged by size:
2162 907 528 500
PciI A'CATG_T
Cuts at: 2553 2553
Size: 4097
PflMI CCAn_nnn'nTGG
Cuts at: 642 1029 642
Size: 387 3710
PfoI T'CCnGG_A
Cuts at: 907 938 1022 907
Size: 31 84 3982
Fragments arranged by size:
3982 84 31
PmeI GTTT'AAAC
Cuts at: 1096 1096
Size: 4097
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3236 3268 3236
Size: 32 4065
PpuMI rG'GwC_Cy
Cuts at: 825 1043 1052 825
Size: 218 9 3870
Fragments arranged by size:
3870 218 9
PstI C_TGCA'G
Cuts at: 210 494 656 1144 1829 210
Size: 284 162 488 685 2478
Fragments arranged by size:
2478 685 488 284 162
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4097
PvuII CAG'CTG
Cuts at: 552 795 1522 1882 552
Size: 243 727 360 2767
Fragments arranged by size:
2767 727 360 243
RsrII CG'GwC_CG
Cuts at: 2292 2292
Size: 4097
SacI G_AGCT'C
Cuts at: 662 1111 662
Size: 449 3648
SalI G'TCGA_C
Cuts at: 1134 1134
Size: 4097
SapI GCTCTTCn'nnn_
Cuts at: 2120 2330 2120
Size: 210 3887
SbfI CC_TGCA'GG
Cuts at: 1144 1144
Size: 4097
ScaI AGT'ACT
Cuts at: 3614 3614
Size: 4097
SexAI A'CCwGG_T
Cuts at: 197 197
Size: 4097
SfcI C'TryA_G
Cuts at: 33 206 490 652 1140 1825 2818 3009 33
Size: 173 284 162 488 685 993 191 1121
Fragments arranged by size:
1121 993 685 488 284 191 173 162
SfoI GGC'GCC
Cuts at: 1529 1777 1529
Size: 248 3849
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4097
SmaI CCC'GGG
Cuts at: 823 1119 823
Size: 296 3801
SmlI C'TyrA_G
Cuts at: 863 1252 2167 2659 2921 863
Size: 389 915 492 262 2039
Fragments arranged by size:
2039 915 492 389 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4097
SphI G_CATG'C
Cuts at: 622 1150 1650 622
Size: 528 500 3069
Fragments arranged by size:
3069 528 500
StyI C'CwwG_G
Cuts at: 107 483 828 1231 1585 107
Size: 376 345 403 354 2619
Fragments arranged by size:
2619 403 376 354 345
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 717 1680 1813 2272 2481 717
Size: 963 133 459 209 2333
Fragments arranged by size:
2333 963 459 209 133
TatI w'GTAC_w
Cuts at: 3612 3612
Size: 4097
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3614 3646 3614
Size: 32 4065
Tth111I GACn'n_nGTC
Cuts at: 727 1894 727
Size: 1167 2930
XbaI T'CTAG_A
Cuts at: 1128 1128
Size: 4097
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 685 1592 685
Size: 907 3190
XhoI C'TCGA_G
Cuts at: 863 863
Size: 4097
XmaI C'CCGG_G
Cuts at: 821 1117 821
Size: 296 3801
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NsiI NspI PciI PflMI PfoI
PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BglI BmgBI BmtI BsaXI
BsgI BsiWI BsmI BsmBI BsrGI BssHII BstXI BstZ17I
ClaI DraIII EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI PacI PasI PmlI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XmnI ZraI