Restriction map for
pF1KB6493
(Circular) (Six-base) MAPSORT of: pF1KB6493.seq Check: 1994 from: 1 to: 4106
pF1KB6493 4106 bp
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AccI GT'mk_AC
Cuts at: 483 1144 483
Size: 661 3445
Acc65I G'GTAC_C
Cuts at: 1122 1122
Size: 4106
AclI AA'CG_TT
Cuts at: 3946 3946
Size: 4106
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1051 1076 1931 2363 3110 3378 1051
Size: 25 855 432 747 268 1779
Fragments arranged by size:
1779 855 747 432 268 25
AflIII A'CryG_T
Cuts at: 1458 2562 3539 1458
Size: 1104 977 2025
Fragments arranged by size:
2025 1104 977
AgeI A'CCGG_T
Cuts at: 2494 2494
Size: 4106
AlwNI CAG_nnn'CTG
Cuts at: 667 1411 2978 667
Size: 744 1567 1795
Fragments arranged by size:
1795 1567 744
ApaLI G'TGCA_C
Cuts at: 2876 2876
Size: 4106
ApoI r'AATT_y
Cuts at: 613 738 1110 3459 3715 613
Size: 125 372 2349 256 1004
Fragments arranged by size:
2349 1004 372 256 125
AseI AT'TA_AT
Cuts at: 20 440 593 20
Size: 420 153 3533
Fragments arranged by size:
3533 420 153
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4106
AvaI C'yCGr_G
Cuts at: 1126 1126
Size: 4106
BamHI G'GATC_C
Cuts at: 966 1131 966
Size: 165 3941
BanI G'GyrC_C
Cuts at: 1122 1536 1784 1819 1122
Size: 414 248 35 3409
Fragments arranged by size:
3409 414 248 35
BanII G_rGCy'C
Cuts at: 794 1120 2150 794
Size: 326 1030 2750
Fragments arranged by size:
2750 1030 326
BbeI G_GCGC'C
Cuts at: 1540 1788 1540
Size: 248 3858
BbsI GAAGACnn'nnnn_
Cuts at: 873 873
Size: 4106
BciVI GTATCCnnnnn_n'
Cuts at: 2004 2765 4106 2004
Size: 761 1341 2004
Fragments arranged by size:
2004 1341 761
BclI T'GATC_A
Cuts at: 1626 1626
Size: 4106
BglII A'GATC_T
Cuts at: 686 1621 3658 686
Size: 935 2037 1134
Fragments arranged by size:
2037 1134 935
BlpI GC'TnA_GC
Cuts at: 1218 1218
Size: 4106
Bme1580I G_kGCm'C
Cuts at: 115 1731 1824 2880 115
Size: 1616 93 1056 1341
Fragments arranged by size:
1616 1341 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1318 1399 1734 3878 1318
Size: 81 335 2144 1546
Fragments arranged by size:
2144 1546 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2473 2473
Size: 4106
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 530 530
Size: 4106
Bpu10I CC'TnA_GC
Cuts at: 1088 1088
Size: 4106
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1282 2161 2653 2951 1282
Size: 879 492 298 2437
Fragments arranged by size:
2437 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3810 3810
Size: 4106
BsaAI yAC'GTr
Cuts at: 1459 1459
Size: 4106
BsaBI GATnn'nnATC
Cuts at: 6 386 1620 3657 6
Size: 380 1234 2037 455
Fragments arranged by size:
2037 1234 455 380
BsaHI Gr'CG_yC
Cuts at: 1537 1785 2487 3983 1537
Size: 248 702 1496 1660
Fragments arranged by size:
1660 1496 702 248
BsaWI w'CCGG_w
Cuts at: 150 1295 1816 2494 2768 2915 3326 150
Size: 1145 521 678 274 147 411 930
Fragments arranged by size:
1145 930 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 947 977 947
Size: 30 4076
BseYI C'CCAG_C
Cuts at: 679 1531 2289 2866 3362 679
Size: 852 758 577 496 1423
Fragments arranged by size:
1423 852 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1648 1694 2902 104
Size: 1544 46 1208 1308
Fragments arranged by size:
1544 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1120 1898 2088 2880 1120
Size: 778 190 792 2346
Fragments arranged by size:
2346 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 115 794 1120 1731 1824 1898 2088 2150 2880
Size: 679 326 611 93 74 190 62 730
Cuts at: 2880 115
Size: 1341
Fragments arranged by size:
1341 730 679 611 326 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3326 3326
Size: 4106
BspHI T'CATG_A
Cuts at: 3282 3282
Size: 4106
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1156 1370 1672 2053 1156
Size: 214 302 381 3209
Fragments arranged by size:
3209 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2398 2452 3936 4101 50
Size: 2348 54 1484 165 55
Fragments arranged by size:
2348 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2018 2018
Size: 4106
BsrFI r'CCGG_y
Cuts at: 2104 2285 2494 2104
Size: 181 209 3716
Fragments arranged by size:
3716 209 181
BssSI C'ACGA_G
Cuts at: 2377 2735 2377
Size: 358 3748
BstAPI GCAn_nnn'nTGC
Cuts at: 1483 1483
Size: 4106
BstBI TT'CG_AA
Cuts at: 2467 2467
Size: 4106
BstYI r'GATC_y
Cuts at: 1 279 686 966 1131 1598 1621 1956 2202
Size: 278 407 280 165 467 23 335 246
Cuts at: 2202 3214 3300 3312 3658 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 467 449 407 346 335 280 278
246 165 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 783 783
Size: 4106
BtgI C'CryG_G
Cuts at: 107 107
Size: 4106
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 283 2056 2237 2400 283
Size: 1773 181 163 1989
Fragments arranged by size:
1989 1773 181 163
BtsI GCAGTG_nn'
Cuts at: 1349 1349
Size: 4106
DraI TTT'AAA
Cuts at: 1105 1105
Size: 4106
DrdI GACnn_nn'nnGTC
Cuts at: 1812 2670 1812
Size: 858 3248
EaeI y'GGCC_r
Cuts at: 1691 1865 2256 2283 3628 1691
Size: 174 391 27 1345 2169
Fragments arranged by size:
2169 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1691 1691
Size: 4106
EarI CTCTTCn'nnn_
Cuts at: 308 2129 2339 308
Size: 1821 210 2075
Fragments arranged by size:
2075 1821 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2314 2624 2770 3916 2314
Size: 310 146 1146 2504
Fragments arranged by size:
2504 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1118 1118
Size: 4106
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 530 1051 1076 1931 2363 3110 3378 530
Size: 521 25 855 432 747 268 1258
Fragments arranged by size:
1258 855 747 521 432 268 25
EcoO109I rG'GnC_Cy
Cuts at: 840 1245 840
Size: 405 3701
EcoRI G'AATT_C
Cuts at: 1110 1110
Size: 4106
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 560 1081 2125 560
Size: 521 1044 2541
Fragments arranged by size:
2541 1044 521
FspI TGC'GCA
Cuts at: 1887 1887
Size: 4106
HaeII r_GCGC'y
Cuts at: 1540 1788 2810 3789 1540
Size: 248 1022 979 1857
Fragments arranged by size:
1857 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1948 1978 1980 2010 1948
Size: 30 2 30 4044
Fragments arranged by size:
4044 30 30 2
HincII GTy'rAC
Cuts at: 1145 1145
Size: 4106
HindIII A'AGCT_T
Cuts at: 580 580
Size: 4106
KasI G'GCGC_C
Cuts at: 1536 1784 1536
Size: 248 3858
KpnI G_GTAC'C
Cuts at: 1126 1126
Size: 4106
MluI A'CGCG_T
Cuts at: 3539 3539
Size: 4106
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2777 2961 2777
Size: 184 3922
MscI TGG'CCA
Cuts at: 1867 1867
Size: 4106
MslI CAynn'nnrTG
Cuts at: 350 2095 2222 350
Size: 1745 127 2234
Fragments arranged by size:
2234 1745 127
MspA1I CmG'CkG
Cuts at: 1217 1531 1891 2904 3149 1217
Size: 314 360 1013 245 2174
Fragments arranged by size:
2174 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2287 2287
Size: 4106
NarI GG'CG_CC
Cuts at: 1537 1785 1537
Size: 248 3858
NcoI C'CATG_G
Cuts at: 107 107
Size: 4106
NgoMIV G'CCGG_C
Cuts at: 2285 2285
Size: 4106
NspI r_CATG'y
Cuts at: 178 917 1053 1159 1659 2566 178
Size: 739 136 106 500 907 1718
Fragments arranged by size:
1718 907 739 500 136 106
PciI A'CATG_T
Cuts at: 2562 2562
Size: 4106
PmeI GTTT'AAAC
Cuts at: 1105 1105
Size: 4106
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1064 1096 3245 3277 1064
Size: 32 2149 32 1893
Fragments arranged by size:
2149 1893 32 32
PpuMI rG'GwC_Cy
Cuts at: 840 840
Size: 4106
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 761 793 761
Size: 32 4074
PstI C_TGCA'G
Cuts at: 1153 1838 1153
Size: 685 3421
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4106
PvuII CAG'CTG
Cuts at: 1531 1891 1531
Size: 360 3746
RsrII CG'GwC_CG
Cuts at: 2301 2301
Size: 4106
SacI G_AGCT'C
Cuts at: 1120 1120
Size: 4106
SalI G'TCGA_C
Cuts at: 1143 1143
Size: 4106
SapI GCTCTTCn'nnn_
Cuts at: 2129 2339 2129
Size: 210 3896
SbfI CC_TGCA'GG
Cuts at: 1153 1153
Size: 4106
ScaI AGT'ACT
Cuts at: 3623 3623
Size: 4106
SexAI A'CCwGG_T
Cuts at: 660 660
Size: 4106
SfcI C'TryA_G
Cuts at: 33 484 1149 1834 2827 3018 33
Size: 451 665 685 993 191 1121
Fragments arranged by size:
1121 993 685 665 451 191
SfoI GGC'GCC
Cuts at: 1538 1786 1538
Size: 248 3858
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4106
SmaI CCC'GGG
Cuts at: 1128 1128
Size: 4106
SmlI C'TyrA_G
Cuts at: 1261 2176 2668 2930 1261
Size: 915 492 262 2437
Fragments arranged by size:
2437 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4106
SphI G_CATG'C
Cuts at: 1053 1159 1659 1053
Size: 106 500 3500
Fragments arranged by size:
3500 500 106
StyI C'CwwG_G
Cuts at: 107 1240 1594 107
Size: 1133 354 2619
Fragments arranged by size:
2619 1133 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1689 1822 2281 2490 1689
Size: 133 459 209 3305
Fragments arranged by size:
3305 459 209 133
TatI w'GTAC_w
Cuts at: 3621 3621
Size: 4106
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 947 979 3623 3655 947
Size: 32 2644 32 1398
Fragments arranged by size:
2644 1398 32 32
Tth111I GACn'n_nGTC
Cuts at: 1903 1903
Size: 4106
XbaI T'CTAG_A
Cuts at: 1137 1137
Size: 4106
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 349 1601 349
Size: 1252 2854
XmaI C'CCGG_G
Cuts at: 1126 1126
Size: 4106
XmnI GAAnn'nnTTC
Cuts at: 317 1064 317
Size: 747 3359
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PmeI PpiI PpuMI PsrI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmgBI BmtI BseRI BsgI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmlI PshAI
PsiI PspOMI PspXI SacII SanDI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI ZraI