Restriction map for pF1KB6494
 (Circular) (Six-base) MAPSORT of: pF1KB6494.seq  Check: 4622  from: 1  to: 3923



pF1KB6494  3923 bp

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:05  ..

AccI GT'mk_AC

Cuts at:    961     961 
   Size:       3923
 
Acc65I G'GTAC_C

Cuts at:    939     939 
   Size:       3923
 
AclI AA'CG_TT

Cuts at:   3763    3763 
   Size:       3923
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    794    1748    2180    2927    3195     794 
   Size:        954     432     747     268    1522

  Fragments arranged by size: 

                1522     954     747     432     268
 
AflIII A'CryG_T

Cuts at:    815    1275    2379    3356     815 
   Size:        460    1104     977    1382

  Fragments arranged by size: 

                1382    1104     977     460
 
AgeI A'CCGG_T

Cuts at:   2311    2311 
   Size:       3923
 
AleI CACnn'nnGTG

Cuts at:    334     334 
   Size:       3923
 
AlwNI CAG_nnn'CTG

Cuts at:   1228    2795    1228 
   Size:       1567    2356
 
ApaLI G'TGCA_C

Cuts at:   2693    2693 
   Size:       3923
 
ApoI r'AATT_y

Cuts at:    747     927    3276    3532     747 
   Size:        180    2349     256    1138

  Fragments arranged by size: 

                2349    1138     256     180
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3923
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3923
 
AvaI C'yCGr_G

Cuts at:    177     230     943     177 
   Size:         53     713    3157

  Fragments arranged by size: 

                3157     713      53
 
BamHI G'GATC_C

Cuts at:    948     948 
   Size:       3923
 
BanI G'GyrC_C

Cuts at:    939    1353    1601    1636     939 
   Size:        414     248      35    3226

  Fragments arranged by size: 

                3226     414     248      35
 
BanII G_rGCy'C

Cuts at:    231     237     937    1967     231 
   Size:          6     700    1030    2187

  Fragments arranged by size: 

                2187    1030     700       6
 
BbeI G_GCGC'C

Cuts at:   1357    1605    1357 
   Size:        248    3675
 
BbvCI CC'TCA_GC

Cuts at:    636     636 
   Size:       3923
 
BciVI GTATCCnnnnn_n'

Cuts at:    834    1821    2582    3923     834 
   Size:        987     761    1341     834

  Fragments arranged by size: 

                1341     987     834     761
 
BclI T'GATC_A

Cuts at:    593    1443     593 
   Size:        850    3073
 
BglII A'GATC_T

Cuts at:    505    1438    3475     505 
   Size:        933    2037     953

  Fragments arranged by size: 

                2037     953     933
 
BlpI GC'TnA_GC

Cuts at:   1035    1035 
   Size:       3923
 
Bme1580I G_kGCm'C

Cuts at:    251     869    1548    1641    2697     251 
   Size:        618     679      93    1056    1477

  Fragments arranged by size: 

                1477    1056     679     618      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   1135    1216    1551    3695    1135 
   Size:         81     335    2144    1363

  Fragments arranged by size: 

                2144    1363     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2290    2290 
   Size:       3923
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    455     574     455 
   Size:        119    3804
 
Bpu10I CC'TnA_GC

Cuts at:    636     636 
   Size:       3923
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    191     781    1099    1978    2470    2768     191 
   Size:        590     318     879     492     298    1346

  Fragments arranged by size: 

                1346     879     590     492     318     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3627    3627 
   Size:       3923
 
BsaAI yAC'GTr

Cuts at:   1276    1276 
   Size:       3923
 
BsaBI GATnn'nnATC

Cuts at:      6    1437    3474       6 
   Size:       1431    2037     455

  Fragments arranged by size: 

                2037    1431     455
 
BsaHI Gr'CG_yC

Cuts at:   1354    1602    2304    3800    1354 
   Size:        248     702    1496    1477

  Fragments arranged by size: 

                1496    1477     702     248
 
BsaWI w'CCGG_w

Cuts at:   1112    1633    2311    2585    2732    3143    1112 
   Size:        521     678     274     147     411    1892

  Fragments arranged by size: 

                1892     678     521     411     274     147
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    132     132 
   Size:       3923
 
BseYI C'CCAG_C

Cuts at:    236    1348    2106    2683    3179     236 
   Size:       1112     758     577     496     980

  Fragments arranged by size: 

                1112     980     758     577     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    389     389 
   Size:       3923
 
BsiEI CG_ry'CG

Cuts at:    104    1465    1511    2719     104 
   Size:       1361      46    1208    1308

  Fragments arranged by size: 

                1361    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    651     937    1715    1905    2697     651 
   Size:        286     778     190     792    1877

  Fragments arranged by size: 

                1877     792     778     286     190
 
Bsp1286I G_dGCh'C

Cuts at:    231     237     251     651     869     937    1548    1641    1715 
   Size:          6      14     400     218      68     611      93      74

Cuts at:   1715    1905    1967    2697     231 
   Size:        190      62     730    1457

  Fragments arranged by size: 

                1457     730     611     400     218     190      93      74
                  68      62      14       6
 
BspEI T'CCGG_A

Cuts at:   3143    3143 
   Size:       3923
 
BspHI T'CATG_A

Cuts at:   3099    3099 
   Size:       3923
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    973    1187    1489    1870     973 
   Size:        214     302     381    3026

  Fragments arranged by size: 

                3026     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2215    2269    3753    3918      50 
   Size:       2165      54    1484     165      55

  Fragments arranged by size: 

                2165    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    864    1835     864 
   Size:        971    2952
 
BsrFI r'CCGG_y

Cuts at:    157    1921    2102    2311     157 
   Size:       1764     181     209    1769

  Fragments arranged by size: 

                1769    1764     209     181
 
BssSI C'ACGA_G

Cuts at:   2194    2552    2194 
   Size:        358    3565
 
BstAPI GCAn_nnn'nTGC

Cuts at:    246    1300     246 
   Size:       1054    2869
 
BstBI TT'CG_AA

Cuts at:    125    2284     125 
   Size:       2159    1764
 
BstYI r'GATC_y

Cuts at:      1     505     792     948    1415    1438    1773    2019    3031 
   Size:        504     287     156     467      23     335     246    1012

Cuts at:   3031    3117    3129    3475       1 
   Size:         86      12     346     449

  Fragments arranged by size: 

                1012     504     467     449     346     335     287     246
                 156      86      23      12
 
BtgI C'CryG_G

Cuts at:    410     410 
   Size:       3923
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1873    2054    2217    1873 
   Size:        181     163    3579

  Fragments arranged by size: 

                3579     181     163
 
BtsI GCAGTG_nn'

Cuts at:    456    1166     456 
   Size:        710    3213
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    267     302     267 
   Size:         35    3888
 
DraI TTT'AAA

Cuts at:    900     922     900 
   Size:         22    3901
 
DraIII CAC_nnn'GTG

Cuts at:    246     246 
   Size:       3923
 
DrdI GACnn_nn'nnGTC

Cuts at:   1629    2487    1629 
   Size:        858    3065
 
EaeI y'GGCC_r

Cuts at:   1508    1682    2073    2100    3445    1508 
   Size:        174     391      27    1345    1986

  Fragments arranged by size: 

                1986    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   1508    1508 
   Size:       3923
 
EarI CTCTTCn'nnn_

Cuts at:   1946    2156    1946 
   Size:        210    3713
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2131    2441    2587    3733    2131 
   Size:        310     146    1146    2321

  Fragments arranged by size: 

                2321    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    935     935 
   Size:       3923
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    455     574     794    1748    2180    2927    3195     455 
   Size:        119     220     954     432     747     268    1183

  Fragments arranged by size: 

                1183     954     747     432     268     220     119
 
EcoO109I rG'GnC_Cy

Cuts at:   1062    1062 
   Size:       3923
 
EcoRI G'AATT_C

Cuts at:    927     927 
   Size:       3923
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1942    1942 
   Size:       3923
 
FspI TGC'GCA

Cuts at:   1704    1704 
   Size:       3923
 
HaeII r_GCGC'y

Cuts at:   1357    1605    2627    3606    1357 
   Size:        248    1022     979    1674

  Fragments arranged by size: 

                1674    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    638     670    1765    1795    1797    1827     638 
   Size:         32    1095      30       2      30    2734

  Fragments arranged by size: 

                2734    1095      32      30      30       2
 
HincII GTy'rAC

Cuts at:    432     962     432 
   Size:        530    3393
 
KasI G'GCGC_C

Cuts at:   1353    1601    1353 
   Size:        248    3675
 
KpnI G_GTAC'C

Cuts at:    943     943 
   Size:       3923
 
MluI A'CGCG_T

Cuts at:   3356    3356 
   Size:       3923
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    391    2594    2778     391 
   Size:       2203     184    1536

  Fragments arranged by size: 

                2203    1536     184
 
MscI TGG'CCA

Cuts at:   1684    1684 
   Size:       3923
 
MslI CAynn'nnrTG

Cuts at:    334    1912    2039     334 
   Size:       1578     127    2218

  Fragments arranged by size: 

                2218    1578     127
 
MspA1I CmG'CkG

Cuts at:    267     403     807    1034    1348    1708    2721    2966     267 
   Size:        136     404     227     314     360    1013     245    1224

  Fragments arranged by size: 

                1224    1013     404     360     314     245     227     136
            
 
NaeI GCC'GGC

Cuts at:    159    2104     159 
   Size:       1945    1978
 
NarI GG'CG_CC

Cuts at:   1354    1602    1354 
   Size:        248    3675
 
NcoI C'CATG_G

Cuts at:    410     410 
   Size:       3923
 
NdeI CA'TA_TG

Cuts at:    480     480 
   Size:       3923
 
NgoMIV G'CCGG_C

Cuts at:    157    2102     157 
   Size:       1945    1978
 
NspI r_CATG'y

Cuts at:    355     819     976    1476    2383     355 
   Size:        464     157     500     907    1895

  Fragments arranged by size: 

                1895     907     500     464     157
 
PciI A'CATG_T

Cuts at:    815    2379     815 
   Size:       1564    2359
 
PflMI CCAn_nnn'nTGG

Cuts at:    715     715 
   Size:       3923
 
PfoI T'CCnGG_A

Cuts at:    663     663 
   Size:       3923
 
PmeI GTTT'AAAC

Cuts at:    922     922 
   Size:       3923
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3062    3094    3062 
   Size:         32    3891
 
PstI C_TGCA'G

Cuts at:    970    1655     970 
   Size:        685    3238
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3923
 
PvuII CAG'CTG

Cuts at:    267     403     807    1348    1708     267 
   Size:        136     404     541     360    2482

  Fragments arranged by size: 

                2482     541     404     360     136
 
RsrII CG'GwC_CG

Cuts at:   2118    2118 
   Size:       3923
 
SacI G_AGCT'C

Cuts at:    937     937 
   Size:       3923
 
SalI G'TCGA_C

Cuts at:    960     960 
   Size:       3923
 
SapI GCTCTTCn'nnn_

Cuts at:   1946    2156    1946 
   Size:        210    3713
 
SbfI CC_TGCA'GG

Cuts at:    970     970 
   Size:       3923
 
ScaI AGT'ACT

Cuts at:   3440    3440 
   Size:       3923
 
SexAI A'CCwGG_T

Cuts at:    737     737 
   Size:       3923
 
SfcI C'TryA_G

Cuts at:     33     966    1651    2644    2835      33 
   Size:        933     685     993     191    1121

  Fragments arranged by size: 

                1121     993     933     685     191
 
SfoI GGC'GCC

Cuts at:   1355    1603    1355 
   Size:        248    3675
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3923
 
SmaI CCC'GGG

Cuts at:    179     945     179 
   Size:        766    3157
 
SmlI C'TyrA_G

Cuts at:    206     796    1078    1993    2485    2747     206 
   Size:        590     282     915     492     262    1382

  Fragments arranged by size: 

                1382     915     590     492     282     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3923
 
SphI G_CATG'C

Cuts at:    976    1476     976 
   Size:        500    3423
 
StyI C'CwwG_G

Cuts at:    410    1057    1411     410 
   Size:        647     354    2922

  Fragments arranged by size: 

                2922     647     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    423    1506    1639    2098    2307     423 
   Size:       1083     133     459     209    2039

  Fragments arranged by size: 

                2039    1083     459     209     133
 
TatI w'GTAC_w

Cuts at:    253    3438     253 
   Size:       3185     738
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    450     482     675     707    3440    3472     450 
   Size:         32     193      32    2733      32     901

  Fragments arranged by size: 

                2733     901     193      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1720    1720 
   Size:       3923
 
XbaI T'CTAG_A

Cuts at:    954     954 
   Size:       3923
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    582    1418     582 
   Size:        836    3087
 
XmaI C'CCGG_G

Cuts at:    177     943     177 
   Size:        766    3157
 
XmnI GAAnn'nnTTC

Cuts at:    568     700     568 
   Size:        132    3791
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AleI    AlwNI
    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII
     BbeI    BbvCI    BciVI     BclI    BglII     BlpI Bme1580I     BmrI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI
    BstYI     BtgI    BtgZI     BtsI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI
     FalI     FspI    HaeII    Hin4I   HincII     KasI     KpnI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NspI     PciI    PflMI     PfoI     PmeI     PpiI     PstI
     PvuI    PvuII    RsrII     SacI     SalI     SapI     SbfI     ScaI
    SexAI     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI     SphI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AlfI     AloI     ApaI
     AscI    AvrII     BaeI     BbsI     BcgI    BfrBI     BglI    BmgBI
     BmtI    BsaXI    BsiWI     BsmI    BsmBI    BsrGI   BssHII   BstEII
    BstXI  BstZ17I   Bsu36I     ClaI    EcoNI    EcoRV     FseI    FspAI
  HindIII     HpaI     MfeI     NheI     NotI     NruI     NsiI     PacI
     PasI     PmlI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
    SacII    SanDI     SfiI    SgrAI    SnaBI     SrfI     SspI     StuI
     SwaI     XhoI     ZraI
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