Restriction map for
pF1KB6494
(Circular) (Six-base) MAPSORT of: pF1KB6494.seq Check: 4622 from: 1 to: 3923
pF1KB6494 3923 bp
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AccI GT'mk_AC
Cuts at: 961 961
Size: 3923
Acc65I G'GTAC_C
Cuts at: 939 939
Size: 3923
AclI AA'CG_TT
Cuts at: 3763 3763
Size: 3923
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 794 1748 2180 2927 3195 794
Size: 954 432 747 268 1522
Fragments arranged by size:
1522 954 747 432 268
AflIII A'CryG_T
Cuts at: 815 1275 2379 3356 815
Size: 460 1104 977 1382
Fragments arranged by size:
1382 1104 977 460
AgeI A'CCGG_T
Cuts at: 2311 2311
Size: 3923
AleI CACnn'nnGTG
Cuts at: 334 334
Size: 3923
AlwNI CAG_nnn'CTG
Cuts at: 1228 2795 1228
Size: 1567 2356
ApaLI G'TGCA_C
Cuts at: 2693 2693
Size: 3923
ApoI r'AATT_y
Cuts at: 747 927 3276 3532 747
Size: 180 2349 256 1138
Fragments arranged by size:
2349 1138 256 180
AseI AT'TA_AT
Cuts at: 20 20
Size: 3923
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3923
AvaI C'yCGr_G
Cuts at: 177 230 943 177
Size: 53 713 3157
Fragments arranged by size:
3157 713 53
BamHI G'GATC_C
Cuts at: 948 948
Size: 3923
BanI G'GyrC_C
Cuts at: 939 1353 1601 1636 939
Size: 414 248 35 3226
Fragments arranged by size:
3226 414 248 35
BanII G_rGCy'C
Cuts at: 231 237 937 1967 231
Size: 6 700 1030 2187
Fragments arranged by size:
2187 1030 700 6
BbeI G_GCGC'C
Cuts at: 1357 1605 1357
Size: 248 3675
BbvCI CC'TCA_GC
Cuts at: 636 636
Size: 3923
BciVI GTATCCnnnnn_n'
Cuts at: 834 1821 2582 3923 834
Size: 987 761 1341 834
Fragments arranged by size:
1341 987 834 761
BclI T'GATC_A
Cuts at: 593 1443 593
Size: 850 3073
BglII A'GATC_T
Cuts at: 505 1438 3475 505
Size: 933 2037 953
Fragments arranged by size:
2037 953 933
BlpI GC'TnA_GC
Cuts at: 1035 1035
Size: 3923
Bme1580I G_kGCm'C
Cuts at: 251 869 1548 1641 2697 251
Size: 618 679 93 1056 1477
Fragments arranged by size:
1477 1056 679 618 93
BmrI ACTGGGnnnn_n'
Cuts at: 1135 1216 1551 3695 1135
Size: 81 335 2144 1363
Fragments arranged by size:
2144 1363 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2290 2290
Size: 3923
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 455 574 455
Size: 119 3804
Bpu10I CC'TnA_GC
Cuts at: 636 636
Size: 3923
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 191 781 1099 1978 2470 2768 191
Size: 590 318 879 492 298 1346
Fragments arranged by size:
1346 879 590 492 318 298
BsaI GGTCTCn'nnnn_
Cuts at: 3627 3627
Size: 3923
BsaAI yAC'GTr
Cuts at: 1276 1276
Size: 3923
BsaBI GATnn'nnATC
Cuts at: 6 1437 3474 6
Size: 1431 2037 455
Fragments arranged by size:
2037 1431 455
BsaHI Gr'CG_yC
Cuts at: 1354 1602 2304 3800 1354
Size: 248 702 1496 1477
Fragments arranged by size:
1496 1477 702 248
BsaWI w'CCGG_w
Cuts at: 1112 1633 2311 2585 2732 3143 1112
Size: 521 678 274 147 411 1892
Fragments arranged by size:
1892 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 132 132
Size: 3923
BseYI C'CCAG_C
Cuts at: 236 1348 2106 2683 3179 236
Size: 1112 758 577 496 980
Fragments arranged by size:
1112 980 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 389 389
Size: 3923
BsiEI CG_ry'CG
Cuts at: 104 1465 1511 2719 104
Size: 1361 46 1208 1308
Fragments arranged by size:
1361 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 651 937 1715 1905 2697 651
Size: 286 778 190 792 1877
Fragments arranged by size:
1877 792 778 286 190
Bsp1286I G_dGCh'C
Cuts at: 231 237 251 651 869 937 1548 1641 1715
Size: 6 14 400 218 68 611 93 74
Cuts at: 1715 1905 1967 2697 231
Size: 190 62 730 1457
Fragments arranged by size:
1457 730 611 400 218 190 93 74
68 62 14 6
BspEI T'CCGG_A
Cuts at: 3143 3143
Size: 3923
BspHI T'CATG_A
Cuts at: 3099 3099
Size: 3923
BspMI ACCTGCnnnn'nnnn_
Cuts at: 973 1187 1489 1870 973
Size: 214 302 381 3026
Fragments arranged by size:
3026 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2215 2269 3753 3918 50
Size: 2165 54 1484 165 55
Fragments arranged by size:
2165 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 864 1835 864
Size: 971 2952
BsrFI r'CCGG_y
Cuts at: 157 1921 2102 2311 157
Size: 1764 181 209 1769
Fragments arranged by size:
1769 1764 209 181
BssSI C'ACGA_G
Cuts at: 2194 2552 2194
Size: 358 3565
BstAPI GCAn_nnn'nTGC
Cuts at: 246 1300 246
Size: 1054 2869
BstBI TT'CG_AA
Cuts at: 125 2284 125
Size: 2159 1764
BstYI r'GATC_y
Cuts at: 1 505 792 948 1415 1438 1773 2019 3031
Size: 504 287 156 467 23 335 246 1012
Cuts at: 3031 3117 3129 3475 1
Size: 86 12 346 449
Fragments arranged by size:
1012 504 467 449 346 335 287 246
156 86 23 12
BtgI C'CryG_G
Cuts at: 410 410
Size: 3923
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1873 2054 2217 1873
Size: 181 163 3579
Fragments arranged by size:
3579 181 163
BtsI GCAGTG_nn'
Cuts at: 456 1166 456
Size: 710 3213
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 267 302 267
Size: 35 3888
DraI TTT'AAA
Cuts at: 900 922 900
Size: 22 3901
DraIII CAC_nnn'GTG
Cuts at: 246 246
Size: 3923
DrdI GACnn_nn'nnGTC
Cuts at: 1629 2487 1629
Size: 858 3065
EaeI y'GGCC_r
Cuts at: 1508 1682 2073 2100 3445 1508
Size: 174 391 27 1345 1986
Fragments arranged by size:
1986 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1508 1508
Size: 3923
EarI CTCTTCn'nnn_
Cuts at: 1946 2156 1946
Size: 210 3713
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2131 2441 2587 3733 2131
Size: 310 146 1146 2321
Fragments arranged by size:
2321 1146 310 146
EcoICRI GAG'CTC
Cuts at: 935 935
Size: 3923
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 455 574 794 1748 2180 2927 3195 455
Size: 119 220 954 432 747 268 1183
Fragments arranged by size:
1183 954 747 432 268 220 119
EcoO109I rG'GnC_Cy
Cuts at: 1062 1062
Size: 3923
EcoRI G'AATT_C
Cuts at: 927 927
Size: 3923
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1942 1942
Size: 3923
FspI TGC'GCA
Cuts at: 1704 1704
Size: 3923
HaeII r_GCGC'y
Cuts at: 1357 1605 2627 3606 1357
Size: 248 1022 979 1674
Fragments arranged by size:
1674 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 638 670 1765 1795 1797 1827 638
Size: 32 1095 30 2 30 2734
Fragments arranged by size:
2734 1095 32 30 30 2
HincII GTy'rAC
Cuts at: 432 962 432
Size: 530 3393
KasI G'GCGC_C
Cuts at: 1353 1601 1353
Size: 248 3675
KpnI G_GTAC'C
Cuts at: 943 943
Size: 3923
MluI A'CGCG_T
Cuts at: 3356 3356
Size: 3923
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 391 2594 2778 391
Size: 2203 184 1536
Fragments arranged by size:
2203 1536 184
MscI TGG'CCA
Cuts at: 1684 1684
Size: 3923
MslI CAynn'nnrTG
Cuts at: 334 1912 2039 334
Size: 1578 127 2218
Fragments arranged by size:
2218 1578 127
MspA1I CmG'CkG
Cuts at: 267 403 807 1034 1348 1708 2721 2966 267
Size: 136 404 227 314 360 1013 245 1224
Fragments arranged by size:
1224 1013 404 360 314 245 227 136
NaeI GCC'GGC
Cuts at: 159 2104 159
Size: 1945 1978
NarI GG'CG_CC
Cuts at: 1354 1602 1354
Size: 248 3675
NcoI C'CATG_G
Cuts at: 410 410
Size: 3923
NdeI CA'TA_TG
Cuts at: 480 480
Size: 3923
NgoMIV G'CCGG_C
Cuts at: 157 2102 157
Size: 1945 1978
NspI r_CATG'y
Cuts at: 355 819 976 1476 2383 355
Size: 464 157 500 907 1895
Fragments arranged by size:
1895 907 500 464 157
PciI A'CATG_T
Cuts at: 815 2379 815
Size: 1564 2359
PflMI CCAn_nnn'nTGG
Cuts at: 715 715
Size: 3923
PfoI T'CCnGG_A
Cuts at: 663 663
Size: 3923
PmeI GTTT'AAAC
Cuts at: 922 922
Size: 3923
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3062 3094 3062
Size: 32 3891
PstI C_TGCA'G
Cuts at: 970 1655 970
Size: 685 3238
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3923
PvuII CAG'CTG
Cuts at: 267 403 807 1348 1708 267
Size: 136 404 541 360 2482
Fragments arranged by size:
2482 541 404 360 136
RsrII CG'GwC_CG
Cuts at: 2118 2118
Size: 3923
SacI G_AGCT'C
Cuts at: 937 937
Size: 3923
SalI G'TCGA_C
Cuts at: 960 960
Size: 3923
SapI GCTCTTCn'nnn_
Cuts at: 1946 2156 1946
Size: 210 3713
SbfI CC_TGCA'GG
Cuts at: 970 970
Size: 3923
ScaI AGT'ACT
Cuts at: 3440 3440
Size: 3923
SexAI A'CCwGG_T
Cuts at: 737 737
Size: 3923
SfcI C'TryA_G
Cuts at: 33 966 1651 2644 2835 33
Size: 933 685 993 191 1121
Fragments arranged by size:
1121 993 933 685 191
SfoI GGC'GCC
Cuts at: 1355 1603 1355
Size: 248 3675
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3923
SmaI CCC'GGG
Cuts at: 179 945 179
Size: 766 3157
SmlI C'TyrA_G
Cuts at: 206 796 1078 1993 2485 2747 206
Size: 590 282 915 492 262 1382
Fragments arranged by size:
1382 915 590 492 282 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3923
SphI G_CATG'C
Cuts at: 976 1476 976
Size: 500 3423
StyI C'CwwG_G
Cuts at: 410 1057 1411 410
Size: 647 354 2922
Fragments arranged by size:
2922 647 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 423 1506 1639 2098 2307 423
Size: 1083 133 459 209 2039
Fragments arranged by size:
2039 1083 459 209 133
TatI w'GTAC_w
Cuts at: 253 3438 253
Size: 3185 738
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 450 482 675 707 3440 3472 450
Size: 32 193 32 2733 32 901
Fragments arranged by size:
2733 901 193 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1720 1720
Size: 3923
XbaI T'CTAG_A
Cuts at: 954 954
Size: 3923
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 582 1418 582
Size: 836 3087
XmaI C'CCGG_G
Cuts at: 177 943 177
Size: 766 3157
XmnI GAAnn'nnTTC
Cuts at: 568 700 568
Size: 132 3791
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbvCI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NspI PciI PflMI PfoI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbsI BcgI BfrBI BglI BmgBI
BmtI BsaXI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NheI NotI NruI NsiI PacI
PasI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI ZraI