(Circular) (Six-base) MAPSORT of: pF1KB6494.seq Check: 4622 from: 1 to: 3923 pF1KB6494 3923 bp With 182 enzymes: SgfI * April 24, 2009 13:05 .. AccI GT'mk_AC Cuts at: 961 961 Size: 3923 Acc65I G'GTAC_C Cuts at: 939 939 Size: 3923 AclI AA'CG_TT Cuts at: 3763 3763 Size: 3923 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 794 1748 2180 2927 3195 794 Size: 954 432 747 268 1522 Fragments arranged by size: 1522 954 747 432 268 AflIII A'CryG_T Cuts at: 815 1275 2379 3356 815 Size: 460 1104 977 1382 Fragments arranged by size: 1382 1104 977 460 AgeI A'CCGG_T Cuts at: 2311 2311 Size: 3923 AleI CACnn'nnGTG Cuts at: 334 334 Size: 3923 AlwNI CAG_nnn'CTG Cuts at: 1228 2795 1228 Size: 1567 2356 ApaLI G'TGCA_C Cuts at: 2693 2693 Size: 3923 ApoI r'AATT_y Cuts at: 747 927 3276 3532 747 Size: 180 2349 256 1138 Fragments arranged by size: 2349 1138 256 180 AseI AT'TA_AT Cuts at: 20 20 Size: 3923 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 3923 AvaI C'yCGr_G Cuts at: 177 230 943 177 Size: 53 713 3157 Fragments arranged by size: 3157 713 53 BamHI G'GATC_C Cuts at: 948 948 Size: 3923 BanI G'GyrC_C Cuts at: 939 1353 1601 1636 939 Size: 414 248 35 3226 Fragments arranged by size: 3226 414 248 35 BanII G_rGCy'C Cuts at: 231 237 937 1967 231 Size: 6 700 1030 2187 Fragments arranged by size: 2187 1030 700 6 BbeI G_GCGC'C Cuts at: 1357 1605 1357 Size: 248 3675 BbvCI CC'TCA_GC Cuts at: 636 636 Size: 3923 BciVI GTATCCnnnnn_n' Cuts at: 834 1821 2582 3923 834 Size: 987 761 1341 834 Fragments arranged by size: 1341 987 834 761 BclI T'GATC_A Cuts at: 593 1443 593 Size: 850 3073 BglII A'GATC_T Cuts at: 505 1438 3475 505 Size: 933 2037 953 Fragments arranged by size: 2037 953 933 BlpI GC'TnA_GC Cuts at: 1035 1035 Size: 3923 Bme1580I G_kGCm'C Cuts at: 251 869 1548 1641 2697 251 Size: 618 679 93 1056 1477 Fragments arranged by size: 1477 1056 679 618 93 BmrI ACTGGGnnnn_n' Cuts at: 1135 1216 1551 3695 1135 Size: 81 335 2144 1363 Fragments arranged by size: 2144 1363 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2290 2290 Size: 3923 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 455 574 455 Size: 119 3804 Bpu10I CC'TnA_GC Cuts at: 636 636 Size: 3923 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 191 781 1099 1978 2470 2768 191 Size: 590 318 879 492 298 1346 Fragments arranged by size: 1346 879 590 492 318 298 BsaI GGTCTCn'nnnn_ Cuts at: 3627 3627 Size: 3923 BsaAI yAC'GTr Cuts at: 1276 1276 Size: 3923 BsaBI GATnn'nnATC Cuts at: 6 1437 3474 6 Size: 1431 2037 455 Fragments arranged by size: 2037 1431 455 BsaHI Gr'CG_yC Cuts at: 1354 1602 2304 3800 1354 Size: 248 702 1496 1477 Fragments arranged by size: 1496 1477 702 248 BsaWI w'CCGG_w Cuts at: 1112 1633 2311 2585 2732 3143 1112 Size: 521 678 274 147 411 1892 Fragments arranged by size: 1892 678 521 411 274 147 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 132 132 Size: 3923 BseYI C'CCAG_C Cuts at: 236 1348 2106 2683 3179 236 Size: 1112 758 577 496 980 Fragments arranged by size: 1112 980 758 577 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 389 389 Size: 3923 BsiEI CG_ry'CG Cuts at: 104 1465 1511 2719 104 Size: 1361 46 1208 1308 Fragments arranged by size: 1361 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 651 937 1715 1905 2697 651 Size: 286 778 190 792 1877 Fragments arranged by size: 1877 792 778 286 190 Bsp1286I G_dGCh'C Cuts at: 231 237 251 651 869 937 1548 1641 1715 Size: 6 14 400 218 68 611 93 74 Cuts at: 1715 1905 1967 2697 231 Size: 190 62 730 1457 Fragments arranged by size: 1457 730 611 400 218 190 93 74 68 62 14 6 BspEI T'CCGG_A Cuts at: 3143 3143 Size: 3923 BspHI T'CATG_A Cuts at: 3099 3099 Size: 3923 BspMI ACCTGCnnnn'nnnn_ Cuts at: 973 1187 1489 1870 973 Size: 214 302 381 3026 Fragments arranged by size: 3026 381 302 214 BsrBI CCG'CTC Cuts at: 50 2215 2269 3753 3918 50 Size: 2165 54 1484 165 55 Fragments arranged by size: 2165 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 864 1835 864 Size: 971 2952 BsrFI r'CCGG_y Cuts at: 157 1921 2102 2311 157 Size: 1764 181 209 1769 Fragments arranged by size: 1769 1764 209 181 BssSI C'ACGA_G Cuts at: 2194 2552 2194 Size: 358 3565 BstAPI GCAn_nnn'nTGC Cuts at: 246 1300 246 Size: 1054 2869 BstBI TT'CG_AA Cuts at: 125 2284 125 Size: 2159 1764 BstYI r'GATC_y Cuts at: 1 505 792 948 1415 1438 1773 2019 3031 Size: 504 287 156 467 23 335 246 1012 Cuts at: 3031 3117 3129 3475 1 Size: 86 12 346 449 Fragments arranged by size: 1012 504 467 449 346 335 287 246 156 86 23 12 BtgI C'CryG_G Cuts at: 410 410 Size: 3923 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1873 2054 2217 1873 Size: 181 163 3579 Fragments arranged by size: 3579 181 163 BtsI GCAGTG_nn' Cuts at: 456 1166 456 Size: 710 3213 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 267 302 267 Size: 35 3888 DraI TTT'AAA Cuts at: 900 922 900 Size: 22 3901 DraIII CAC_nnn'GTG Cuts at: 246 246 Size: 3923 DrdI GACnn_nn'nnGTC Cuts at: 1629 2487 1629 Size: 858 3065 EaeI y'GGCC_r Cuts at: 1508 1682 2073 2100 3445 1508 Size: 174 391 27 1345 1986 Fragments arranged by size: 1986 1345 391 174 27 EagI C'GGCC_G Cuts at: 1508 1508 Size: 3923 EarI CTCTTCn'nnn_ Cuts at: 1946 2156 1946 Size: 210 3713 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2131 2441 2587 3733 2131 Size: 310 146 1146 2321 Fragments arranged by size: 2321 1146 310 146 EcoICRI GAG'CTC Cuts at: 935 935 Size: 3923 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 455 574 794 1748 2180 2927 3195 455 Size: 119 220 954 432 747 268 1183 Fragments arranged by size: 1183 954 747 432 268 220 119 EcoO109I rG'GnC_Cy Cuts at: 1062 1062 Size: 3923 EcoRI G'AATT_C Cuts at: 927 927 Size: 3923 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 1942 1942 Size: 3923 FspI TGC'GCA Cuts at: 1704 1704 Size: 3923 HaeII r_GCGC'y Cuts at: 1357 1605 2627 3606 1357 Size: 248 1022 979 1674 Fragments arranged by size: 1674 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 638 670 1765 1795 1797 1827 638 Size: 32 1095 30 2 30 2734 Fragments arranged by size: 2734 1095 32 30 30 2 HincII GTy'rAC Cuts at: 432 962 432 Size: 530 3393 KasI G'GCGC_C Cuts at: 1353 1601 1353 Size: 248 3675 KpnI G_GTAC'C Cuts at: 943 943 Size: 3923 MluI A'CGCG_T Cuts at: 3356 3356 Size: 3923 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 391 2594 2778 391 Size: 2203 184 1536 Fragments arranged by size: 2203 1536 184 MscI TGG'CCA Cuts at: 1684 1684 Size: 3923 MslI CAynn'nnrTG Cuts at: 334 1912 2039 334 Size: 1578 127 2218 Fragments arranged by size: 2218 1578 127 MspA1I CmG'CkG Cuts at: 267 403 807 1034 1348 1708 2721 2966 267 Size: 136 404 227 314 360 1013 245 1224 Fragments arranged by size: 1224 1013 404 360 314 245 227 136 NaeI GCC'GGC Cuts at: 159 2104 159 Size: 1945 1978 NarI GG'CG_CC Cuts at: 1354 1602 1354 Size: 248 3675 NcoI C'CATG_G Cuts at: 410 410 Size: 3923 NdeI CA'TA_TG Cuts at: 480 480 Size: 3923 NgoMIV G'CCGG_C Cuts at: 157 2102 157 Size: 1945 1978 NspI r_CATG'y Cuts at: 355 819 976 1476 2383 355 Size: 464 157 500 907 1895 Fragments arranged by size: 1895 907 500 464 157 PciI A'CATG_T Cuts at: 815 2379 815 Size: 1564 2359 PflMI CCAn_nnn'nTGG Cuts at: 715 715 Size: 3923 PfoI T'CCnGG_A Cuts at: 663 663 Size: 3923 PmeI GTTT'AAAC Cuts at: 922 922 Size: 3923 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3062 3094 3062 Size: 32 3891 PstI C_TGCA'G Cuts at: 970 1655 970 Size: 685 3238 PvuI CG_AT'CG Cuts at: 104 104 Size: 3923 PvuII CAG'CTG Cuts at: 267 403 807 1348 1708 267 Size: 136 404 541 360 2482 Fragments arranged by size: 2482 541 404 360 136 RsrII CG'GwC_CG Cuts at: 2118 2118 Size: 3923 SacI G_AGCT'C Cuts at: 937 937 Size: 3923 SalI G'TCGA_C Cuts at: 960 960 Size: 3923 SapI GCTCTTCn'nnn_ Cuts at: 1946 2156 1946 Size: 210 3713 SbfI CC_TGCA'GG Cuts at: 970 970 Size: 3923 ScaI AGT'ACT Cuts at: 3440 3440 Size: 3923 SexAI A'CCwGG_T Cuts at: 737 737 Size: 3923 SfcI C'TryA_G Cuts at: 33 966 1651 2644 2835 33 Size: 933 685 993 191 1121 Fragments arranged by size: 1121 993 933 685 191 SfoI GGC'GCC Cuts at: 1355 1603 1355 Size: 248 3675 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 3923 SmaI CCC'GGG Cuts at: 179 945 179 Size: 766 3157 SmlI C'TyrA_G Cuts at: 206 796 1078 1993 2485 2747 206 Size: 590 282 915 492 262 1382 Fragments arranged by size: 1382 915 590 492 282 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 3923 SphI G_CATG'C Cuts at: 976 1476 976 Size: 500 3423 StyI C'CwwG_G Cuts at: 410 1057 1411 410 Size: 647 354 2922 Fragments arranged by size: 2922 647 354 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 423 1506 1639 2098 2307 423 Size: 1083 133 459 209 2039 Fragments arranged by size: 2039 1083 459 209 133 TatI w'GTAC_w Cuts at: 253 3438 253 Size: 3185 738 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 450 482 675 707 3440 3472 450 Size: 32 193 32 2733 32 901 Fragments arranged by size: 2733 901 193 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1720 1720 Size: 3923 XbaI T'CTAG_A Cuts at: 954 954 Size: 3923 XcmI CCAnnnn_n'nnnnTGG Cuts at: 582 1418 582 Size: 836 3087 XmaI C'CCGG_G Cuts at: 177 943 177 Size: 766 3157 XmnI GAAnn'nnTTC Cuts at: 568 700 568 Size: 132 3791 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbvCI BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PciI PflMI PfoI PmeI PpiI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AlfI AloI ApaI AscI AvrII BaeI BbsI BcgI BfrBI BglI BmgBI BmtI BsaXI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NheI NotI NruI NsiI PacI PasI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI ZraI