Restriction map for
pF1KB6495
(Circular) (Six-base) MAPSORT of: pF1KB6495.seq Check: 4596 from: 1 to: 3968
pF1KB6495 3968 bp
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AccI GT'mk_AC
Cuts at: 1006 1006
Size: 3968
Acc65I G'GTAC_C
Cuts at: 984 984
Size: 3968
AclI AA'CG_TT
Cuts at: 3808 3808
Size: 3968
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 617 1793 2225 2972 3240 617
Size: 1176 432 747 268 1345
Fragments arranged by size:
1345 1176 747 432 268
AflIII A'CryG_T
Cuts at: 1320 2424 3401 1320
Size: 1104 977 1887
Fragments arranged by size:
1887 1104 977
AgeI A'CCGG_T
Cuts at: 2356 2356
Size: 3968
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 736 768 736
Size: 32 3936
AlwNI CAG_nnn'CTG
Cuts at: 1273 2840 1273
Size: 1567 2401
ApaLI G'TGCA_C
Cuts at: 2738 2738
Size: 3968
ApoI r'AATT_y
Cuts at: 382 972 3321 3577 382
Size: 590 2349 256 773
Fragments arranged by size:
2349 773 590 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3968
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3968
AvaI C'yCGr_G
Cuts at: 249 988 249
Size: 739 3229
BamHI G'GATC_C
Cuts at: 417 669 993 417
Size: 252 324 3392
Fragments arranged by size:
3392 324 252
BanI G'GyrC_C
Cuts at: 148 211 243 268 984 1398 1646 1681 148
Size: 63 32 25 716 414 248 35 2435
Fragments arranged by size:
2435 716 414 248 63 35 32 25
BanII G_rGCy'C
Cuts at: 982 2012 982
Size: 1030 2938
BbeI G_GCGC'C
Cuts at: 152 215 247 272 1402 1650 152
Size: 63 32 25 1130 248 2470
Fragments arranged by size:
2470 1130 248 63 32 25
BciVI GTATCCnnnnn_n'
Cuts at: 339 1866 2627 3968 339
Size: 1527 761 1341 339
Fragments arranged by size:
1527 1341 761 339
BclI T'GATC_A
Cuts at: 1488 1488
Size: 3968
BglII A'GATC_T
Cuts at: 1483 3520 1483
Size: 2037 1931
BlpI GC'TnA_GC
Cuts at: 1080 1080
Size: 3968
Bme1580I G_kGCm'C
Cuts at: 1593 1686 2742 1593
Size: 93 1056 2819
Fragments arranged by size:
2819 1056 93
BmgBI CAC'GTC
Cuts at: 931 931
Size: 3968
BmrI ACTGGGnnnn_n'
Cuts at: 1180 1261 1596 3740 1180
Size: 81 335 2144 1408
Fragments arranged by size:
2144 1408 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2335 2335
Size: 3968
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 248 763 248
Size: 515 3453
Bpu10I CC'TnA_GC
Cuts at: 337 337
Size: 3968
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1144 2023 2515 2813 1144
Size: 879 492 298 2299
Fragments arranged by size:
2299 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3672 3672
Size: 3968
BsaAI yAC'GTr
Cuts at: 1321 1321
Size: 3968
BsaBI GATnn'nnATC
Cuts at: 6 1482 3519 6
Size: 1476 2037 455
Fragments arranged by size:
2037 1476 455
BsaHI Gr'CG_yC
Cuts at: 149 212 244 269 1399 1647 2349 3845 149
Size: 63 32 25 1130 248 702 1496 272
Fragments arranged by size:
1496 1130 702 272 248 63 32 25
BsaWI w'CCGG_w
Cuts at: 1157 1678 2356 2630 2777 3188 1157
Size: 521 678 274 147 411 1937
Fragments arranged by size:
1937 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 380 410 736 766 380
Size: 30 326 30 3582
Fragments arranged by size:
3582 326 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 139 319 139
Size: 180 3788
BseYI C'CCAG_C
Cuts at: 341 794 1393 2151 2728 3224 341
Size: 453 599 758 577 496 1085
Fragments arranged by size:
1085 758 599 577 496 453
BsiEI CG_ry'CG
Cuts at: 104 197 239 1510 1556 2764 104
Size: 93 42 1271 46 1208 1308
Fragments arranged by size:
1308 1271 1208 93 46 42
BsiHKAI G_wGCw'C
Cuts at: 506 982 1760 1950 2742 506
Size: 476 778 190 792 1732
Fragments arranged by size:
1732 792 778 476 190
BsmBI CGTCTCn'nnnn_
Cuts at: 133 133
Size: 3968
Bsp1286I G_dGCh'C
Cuts at: 506 982 1593 1686 1760 1950 2012 2742 506
Size: 476 611 93 74 190 62 730 1732
Fragments arranged by size:
1732 730 611 476 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3188 3188
Size: 3968
BspHI T'CATG_A
Cuts at: 3144 3144
Size: 3968
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1018 1232 1534 1915 1018
Size: 214 302 381 3071
Fragments arranged by size:
3071 381 302 214
BsrBI CCG'CTC
Cuts at: 50 254 2260 2314 3798 3963 50
Size: 204 2006 54 1484 165 55
Fragments arranged by size:
2006 1484 204 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1880 1880
Size: 3968
BsrFI r'CCGG_y
Cuts at: 192 1966 2147 2356 192
Size: 1774 181 209 1804
Fragments arranged by size:
1804 1774 209 181
BsrGI T'GTAC_A
Cuts at: 403 403
Size: 3968
BssSI C'ACGA_G
Cuts at: 2239 2597 2239
Size: 358 3610
BstAPI GCAn_nnn'nTGC
Cuts at: 1345 1345
Size: 3968
BstBI TT'CG_AA
Cuts at: 2329 2329
Size: 3968
BstXI CCAn_nnnn'nTGG
Cuts at: 931 931
Size: 3968
BstYI r'GATC_y
Cuts at: 1 417 669 993 1460 1483 1818 2064 3076
Size: 416 252 324 467 23 335 246 1012
Cuts at: 3076 3162 3174 3520 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 449 416 346 335 324 252
246 86 23 12
BtgI C'CryG_G
Cuts at: 134 215 239 542 840 134
Size: 81 24 303 298 3262
Fragments arranged by size:
3262 303 298 81 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1918 2099 2262 1918
Size: 181 163 3624
Fragments arranged by size:
3624 181 163
BtsI GCAGTG_nn'
Cuts at: 1211 1211
Size: 3968
DraI TTT'AAA
Cuts at: 365 714 967 365
Size: 349 253 3366
Fragments arranged by size:
3366 349 253
DrdI GACnn_nn'nnGTC
Cuts at: 1674 2532 1674
Size: 858 3110
EaeI y'GGCC_r
Cuts at: 194 516 936 1553 1727 2118 2145 3490 194
Size: 322 420 617 174 391 27 1345 672
Fragments arranged by size:
1345 672 617 420 391 322 174 27
EagI C'GGCC_G
Cuts at: 194 1553 194
Size: 1359 2609
EarI CTCTTCn'nnn_
Cuts at: 1991 2201 1991
Size: 210 3758
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2176 2486 2632 3778 2176
Size: 310 146 1146 2366
Fragments arranged by size:
2366 1146 310 146
EcoICRI GAG'CTC
Cuts at: 980 980
Size: 3968
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 248 617 763 1793 2225 2972 3240 248
Size: 369 146 1030 432 747 268 976
Fragments arranged by size:
1030 976 747 432 369 268 146
EcoO109I rG'GnC_Cy
Cuts at: 486 558 1107 486
Size: 72 549 3347
Fragments arranged by size:
3347 549 72
EcoRI G'AATT_C
Cuts at: 972 972
Size: 3968
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 591 1987 591
Size: 1396 2572
FspI TGC'GCA
Cuts at: 1749 1749
Size: 3968
HaeII r_GCGC'y
Cuts at: 152 215 247 272 1402 1650 2672 3651 152
Size: 63 32 25 1130 248 1022 979 469
Fragments arranged by size:
1130 1022 979 469 248 63 32 25
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 243 275 1810 1840 1842 1872 243
Size: 32 1535 30 2 30 2339
Fragments arranged by size:
2339 1535 32 30 30 2
HincII GTy'rAC
Cuts at: 399 1007 399
Size: 608 3360
KasI G'GCGC_C
Cuts at: 148 211 243 268 1398 1646 148
Size: 63 32 25 1130 248 2470
Fragments arranged by size:
2470 1130 248 63 32 25
KpnI G_GTAC'C
Cuts at: 988 988
Size: 3968
MluI A'CGCG_T
Cuts at: 3401 3401
Size: 3968
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 92 2639 2823 92
Size: 2547 184 1237
Fragments arranged by size:
2547 1237 184
MscI TGG'CCA
Cuts at: 938 1729 938
Size: 791 3177
MslI CAynn'nnrTG
Cuts at: 547 1957 2084 547
Size: 1410 127 2431
Fragments arranged by size:
2431 1410 127
MspA1I CmG'CkG
Cuts at: 217 241 872 1079 1393 1753 2766 3011 217
Size: 24 631 207 314 360 1013 245 1174
Fragments arranged by size:
1174 1013 631 360 314 245 207 24
NaeI GCC'GGC
Cuts at: 194 2149 194
Size: 1955 2013
NarI GG'CG_CC
Cuts at: 149 212 244 269 1399 1647 149
Size: 63 32 25 1130 248 2470
Fragments arranged by size:
2470 1130 248 63 32 25
NcoI C'CATG_G
Cuts at: 134 542 134
Size: 408 3560
NgoMIV G'CCGG_C
Cuts at: 192 2147 192
Size: 1955 2013
NspI r_CATG'y
Cuts at: 1021 1521 2428 1021
Size: 500 907 2561
Fragments arranged by size:
2561 907 500
PciI A'CATG_T
Cuts at: 2424 2424
Size: 3968
PmeI GTTT'AAAC
Cuts at: 967 967
Size: 3968
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 736 768 3107 3139 736
Size: 32 2339 32 1565
Fragments arranged by size:
2339 1565 32 32
PpuMI rG'GwC_Cy
Cuts at: 486 558 486
Size: 72 3896
PstI C_TGCA'G
Cuts at: 1015 1700 1015
Size: 685 3283
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3968
PvuII CAG'CTG
Cuts at: 1393 1753 1393
Size: 360 3608
RsrII CG'GwC_CG
Cuts at: 2163 2163
Size: 3968
SacI G_AGCT'C
Cuts at: 982 982
Size: 3968
SacII CC_GC'GG
Cuts at: 218 242 218
Size: 24 3944
SalI G'TCGA_C
Cuts at: 1005 1005
Size: 3968
SapI GCTCTTCn'nnn_
Cuts at: 1991 2201 1991
Size: 210 3758
SbfI CC_TGCA'GG
Cuts at: 1015 1015
Size: 3968
ScaI AGT'ACT
Cuts at: 625 3485 625
Size: 2860 1108
SfcI C'TryA_G
Cuts at: 33 1011 1696 2689 2880 33
Size: 978 685 993 191 1121
Fragments arranged by size:
1121 993 978 685 191
SfoI GGC'GCC
Cuts at: 150 213 245 270 1400 1648 150
Size: 63 32 25 1130 248 2470
Fragments arranged by size:
2470 1130 248 63 32 25
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3968
SmaI CCC'GGG
Cuts at: 990 990
Size: 3968
SmlI C'TyrA_G
Cuts at: 1123 2038 2530 2792 1123
Size: 915 492 262 2299
Fragments arranged by size:
2299 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3968
SphI G_CATG'C
Cuts at: 1021 1521 1021
Size: 500 3468
StyI C'CwwG_G
Cuts at: 134 542 1102 1456 134
Size: 408 560 354 2646
Fragments arranged by size:
2646 560 408 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1551 1684 2143 2352 1551
Size: 133 459 209 3167
Fragments arranged by size:
3167 459 209 133
TatI w'GTAC_w
Cuts at: 403 623 904 3483 403
Size: 220 281 2579 888
Fragments arranged by size:
2579 888 281 220
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3485 3517 3485
Size: 32 3936
Tth111I GACn'n_nGTC
Cuts at: 1765 1765
Size: 3968
XbaI T'CTAG_A
Cuts at: 999 999
Size: 3968
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 763 931 1463 763
Size: 168 532 3268
Fragments arranged by size:
3268 532 168
XmaI C'CCGG_G
Cuts at: 988 988
Size: 3968
XmnI GAAnn'nnTTC
Cuts at: 574 751 574
Size: 177 3791
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PmeI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmtI BsgI BsiWI BsmI BssHII BstEII BstZ17I Bsu36I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PshAI PsiI PspOMI PspXI
PsrI SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI ZraI