Restriction map for
pF1KB6497
(Circular) (Six-base) MAPSORT of: pF1KB6497.seq Check: 6626 from: 1 to: 4166
pF1KB6497 4166 bp
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 642 921 642
Size: 279 3887
AccI GT'mk_AC
Cuts at: 1204 1204
Size: 4166
Acc65I G'GTAC_C
Cuts at: 1182 1182
Size: 4166
AclI AA'CG_TT
Cuts at: 4006 4006
Size: 4166
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 631 1991 2423 3170 3438 631
Size: 1360 432 747 268 1359
Fragments arranged by size:
1360 1359 747 432 268
AfeI AGC'GCT
Cuts at: 949 949
Size: 4166
AflIII A'CryG_T
Cuts at: 1518 2622 3599 1518
Size: 1104 977 2085
Fragments arranged by size:
2085 1104 977
AgeI A'CCGG_T
Cuts at: 2554 2554
Size: 4166
AlwNI CAG_nnn'CTG
Cuts at: 1072 1471 3038 1072
Size: 399 1567 2200
Fragments arranged by size:
2200 1567 399
ApaI G_GGCC'C
Cuts at: 780 780
Size: 4166
ApaLI G'TGCA_C
Cuts at: 2936 2936
Size: 4166
ApoI r'AATT_y
Cuts at: 1170 3519 3775 1170
Size: 2349 256 1561
Fragments arranged by size:
2349 1561 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4166
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4166
AvaI C'yCGr_G
Cuts at: 616 730 1186 616
Size: 114 456 3596
Fragments arranged by size:
3596 456 114
AvrII C'CTAG_G
Cuts at: 780 780
Size: 4166
BamHI G'GATC_C
Cuts at: 1191 1191
Size: 4166
BanI G'GyrC_C
Cuts at: 293 1182 1596 1844 1879 293
Size: 889 414 248 35 2580
Fragments arranged by size:
2580 889 414 248 35
BanII G_rGCy'C
Cuts at: 268 533 780 1115 1180 2210 268
Size: 265 247 335 65 1030 2224
Fragments arranged by size:
2224 1030 335 265 247 65
BbeI G_GCGC'C
Cuts at: 1600 1848 1600
Size: 248 3918
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 517 551 517
Size: 34 4132
BciVI GTATCCnnnnn_n'
Cuts at: 2064 2825 4166 2064
Size: 761 1341 2064
Fragments arranged by size:
2064 1341 761
BclI T'GATC_A
Cuts at: 1686 1686
Size: 4166
BglII A'GATC_T
Cuts at: 845 1681 3718 845
Size: 836 2037 1293
Fragments arranged by size:
2037 1293 836
BlpI GC'TnA_GC
Cuts at: 1278 1278
Size: 4166
Bme1580I G_kGCm'C
Cuts at: 780 1791 1884 2940 780
Size: 1011 93 1056 2006
Fragments arranged by size:
2006 1056 1011 93
BmrI ACTGGGnnnn_n'
Cuts at: 1378 1459 1794 3938 1378
Size: 81 335 2144 1606
Fragments arranged by size:
2144 1606 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 205 513 2533 205
Size: 308 2020 1838
Fragments arranged by size:
2020 1838 308
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 412 412
Size: 4166
Bpu10I CC'TnA_GC
Cuts at: 247 742 247
Size: 495 3671
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 889 1342 2221 2713 3011 889
Size: 453 879 492 298 2044
Fragments arranged by size:
2044 879 492 453 298
BsaI GGTCTCn'nnnn_
Cuts at: 295 3870 295
Size: 3575 591
BsaAI yAC'GTr
Cuts at: 477 1519 477
Size: 1042 3124
BsaBI GATnn'nnATC
Cuts at: 6 1021 1123 1680 3717 6
Size: 1015 102 557 2037 455
Fragments arranged by size:
2037 1015 557 455 102
BsaHI Gr'CG_yC
Cuts at: 1597 1845 2547 4043 1597
Size: 248 702 1496 1720
Fragments arranged by size:
1720 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1115 1355 1876 2554 2828 2975 3386 1115
Size: 240 521 678 274 147 411 1895
Fragments arranged by size:
1895 678 521 411 274 240 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 160 194 616 1117 160
Size: 34 422 501 3209
Fragments arranged by size:
3209 501 422 34
BseYI C'CCAG_C
Cuts at: 255 1591 2349 2926 3422 255
Size: 1336 758 577 496 999
Fragments arranged by size:
1336 999 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 404 1708 1754 2962 104
Size: 300 1304 46 1208 1308
Fragments arranged by size:
1308 1304 1208 300 46
BsiHKAI G_wGCw'C
Cuts at: 371 791 1115 1180 1958 2148 2940 371
Size: 420 324 65 778 190 792 1597
Fragments arranged by size:
1597 792 778 420 324 190 65
BsmBI CGTCTCn'nnnn_
Cuts at: 867 867
Size: 4166
Bsp1286I G_dGCh'C
Cuts at: 268 371 533 780 791 1115 1180 1791 1884
Size: 103 162 247 11 324 65 611 93
Cuts at: 1884 1958 2148 2210 2940 268
Size: 74 190 62 730 1494
Fragments arranged by size:
1494 730 611 324 247 190 162 103
93 74 65 62 11
BspEI T'CCGG_A
Cuts at: 1115 3386 1115
Size: 2271 1895
BspHI T'CATG_A
Cuts at: 3342 3342
Size: 4166
BspMI ACCTGCnnnn'nnnn_
Cuts at: 351 642 656 921 1216 1430 1732 2113 351
Size: 291 14 265 295 214 302 381 2404
Fragments arranged by size:
2404 381 302 295 291 265 214 14
BsrBI CCG'CTC
Cuts at: 50 211 2458 2512 3996 4161 50
Size: 161 2247 54 1484 165 55
Fragments arranged by size:
2247 1484 165 161 55 54
BsrDI GCAATG_nn'
Cuts at: 2078 2078
Size: 4166
BsrFI r'CCGG_y
Cuts at: 304 2164 2345 2554 304
Size: 1860 181 209 1916
Fragments arranged by size:
1916 1860 209 181
BssSI C'ACGA_G
Cuts at: 2437 2795 2437
Size: 358 3808
BstAPI GCAn_nnn'nTGC
Cuts at: 915 1543 915
Size: 628 3538
BstBI TT'CG_AA
Cuts at: 239 2527 239
Size: 2288 1878
BstYI r'GATC_y
Cuts at: 1 326 845 1118 1191 1658 1681 2016 2262
Size: 325 519 273 73 467 23 335 246
Cuts at: 2262 3274 3360 3372 3718 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 519 467 449 346 335 325 273
246 86 73 23 12
Bsu36I CC'TnA_GG
Cuts at: 961 961
Size: 4166
BtgI C'CryG_G
Cuts at: 107 764 806 107
Size: 657 42 3467
Fragments arranged by size:
3467 657 42
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2116 2297 2460 2116
Size: 181 163 3822
Fragments arranged by size:
3822 181 163
BtsI GCAGTG_nn'
Cuts at: 1409 1409
Size: 4166
DraI TTT'AAA
Cuts at: 1165 1165
Size: 4166
DrdI GACnn_nn'nnGTC
Cuts at: 1872 2730 1872
Size: 858 3308
EaeI y'GGCC_r
Cuts at: 309 566 803 1751 1925 2316 2343 3688 309
Size: 257 237 948 174 391 27 1345 787
Fragments arranged by size:
1345 948 787 391 257 237 174 27
EagI C'GGCC_G
Cuts at: 1751 1751
Size: 4166
EarI CTCTTCn'nnn_
Cuts at: 1103 2189 2399 1103
Size: 1086 210 2870
Fragments arranged by size:
2870 1086 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2374 2684 2830 3976 2374
Size: 310 146 1146 2564
Fragments arranged by size:
2564 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1113 1178 1113
Size: 65 4101
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 412 631 1991 2423 3170 3438 412
Size: 219 1360 432 747 268 1140
Fragments arranged by size:
1360 1140 747 432 268 219
EcoNI CCTnn'n_nnAGG
Cuts at: 928 928
Size: 4166
EcoO109I rG'GnC_Cy
Cuts at: 710 777 1069 1305 710
Size: 67 292 236 3571
Fragments arranged by size:
3571 292 236 67
EcoRI G'AATT_C
Cuts at: 1170 1170
Size: 4166
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2185 2185
Size: 4166
FspI TGC'GCA
Cuts at: 1947 1947
Size: 4166
HaeII r_GCGC'y
Cuts at: 951 1600 1848 2870 3849 951
Size: 649 248 1022 979 1268
Fragments arranged by size:
1268 1022 979 649 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2008 2038 2040 2070 2008
Size: 30 2 30 4104
Fragments arranged by size:
4104 30 30 2
HincII GTy'rAC
Cuts at: 1205 1205
Size: 4166
KasI G'GCGC_C
Cuts at: 1596 1844 1596
Size: 248 3918
KpnI G_GTAC'C
Cuts at: 1186 1186
Size: 4166
MluI A'CGCG_T
Cuts at: 3599 3599
Size: 4166
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 495 2837 3021 495
Size: 2342 184 1640
Fragments arranged by size:
2342 1640 184
MscI TGG'CCA
Cuts at: 1927 1927
Size: 4166
MslI CAynn'nnrTG
Cuts at: 2155 2282 2155
Size: 127 4039
MspA1I CmG'CkG
Cuts at: 1013 1277 1591 1951 2964 3209 1013
Size: 264 314 360 1013 245 1970
Fragments arranged by size:
1970 1013 360 314 264 245
NaeI GCC'GGC
Cuts at: 2347 2347
Size: 4166
NarI GG'CG_CC
Cuts at: 1597 1845 1597
Size: 248 3918
NcoI C'CATG_G
Cuts at: 107 107
Size: 4166
NgoMIV G'CCGG_C
Cuts at: 2345 2345
Size: 4166
NspI r_CATG'y
Cuts at: 1219 1719 2626 1219
Size: 500 907 2759
Fragments arranged by size:
2759 907 500
PasI CC'CwG_GG
Cuts at: 1072 1072
Size: 4166
PciI A'CATG_T
Cuts at: 2622 2622
Size: 4166
PmeI GTTT'AAAC
Cuts at: 1165 1165
Size: 4166
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3305 3337 3305
Size: 32 4134
PpuMI rG'GwC_Cy
Cuts at: 710 710
Size: 4166
PspOMI G'GGCC_C
Cuts at: 776 776
Size: 4166
PstI C_TGCA'G
Cuts at: 398 653 932 1213 1898 398
Size: 255 279 281 685 2666
Fragments arranged by size:
2666 685 281 279 255
PvuI CG_AT'CG
Cuts at: 104 404 104
Size: 300 3866
PvuII CAG'CTG
Cuts at: 1013 1591 1951 1013
Size: 578 360 3228
Fragments arranged by size:
3228 578 360
RsrII CG'GwC_CG
Cuts at: 2361 2361
Size: 4166
SacI G_AGCT'C
Cuts at: 1115 1180 1115
Size: 65 4101
SalI G'TCGA_C
Cuts at: 1203 1203
Size: 4166
SanDI GG'GwC_CC
Cuts at: 710 710
Size: 4166
SapI GCTCTTCn'nnn_
Cuts at: 1103 2189 2399 1103
Size: 1086 210 2870
Fragments arranged by size:
2870 1086 210
SbfI CC_TGCA'GG
Cuts at: 398 653 1213 398
Size: 255 560 3351
Fragments arranged by size:
3351 560 255
ScaI AGT'ACT
Cuts at: 3683 3683
Size: 4166
SexAI A'CCwGG_T
Cuts at: 461 461
Size: 4166
SfcI C'TryA_G
Cuts at: 33 192 394 505 649 928 1209 1894 2887
Size: 159 202 111 144 279 281 685 993
Cuts at: 2887 3078 33
Size: 191 1121
Fragments arranged by size:
1121 993 685 281 279 202 191 159
144 111
SfoI GGC'GCC
Cuts at: 1598 1846 1598
Size: 248 3918
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4166
SmaI CCC'GGG
Cuts at: 1188 1188
Size: 4166
SmlI C'TyrA_G
Cuts at: 904 1321 2236 2728 2990 904
Size: 417 915 492 262 2080
Fragments arranged by size:
2080 915 492 417 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4166
SphI G_CATG'C
Cuts at: 1219 1719 1219
Size: 500 3666
StyI C'CwwG_G
Cuts at: 107 780 1142 1300 1654 107
Size: 673 362 158 354 2619
Fragments arranged by size:
2619 673 362 354 158
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1749 1882 2341 2550 1749
Size: 133 459 209 3365
Fragments arranged by size:
3365 459 209 133
TatI w'GTAC_w
Cuts at: 1061 3681 1061
Size: 2620 1546
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3683 3715 3683
Size: 32 4134
Tth111I GACn'n_nGTC
Cuts at: 1963 1963
Size: 4166
XbaI T'CTAG_A
Cuts at: 1197 1197
Size: 4166
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1661 1661
Size: 4166
XmaI C'CCGG_G
Cuts at: 1186 1186
Size: 4166
XmnI GAAnn'nnTTC
Cuts at: 160 160
Size: 4166
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BcgI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PmeI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AleI AlfI AloI AscI BaeI
BbsI BbvCI BfrBI BglI BmgBI BmtI BsaXI BsgI
BsiWI BsmI BsrGI BssHII BstEII BstXI BstZ17I ClaI
CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PflMI PfoI
PmlI PshAI PsiI PspXI PsrI SacII SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI ZraI