Restriction map for
pF1KB6504
(Circular) (Six-base) MAPSORT of: pF1KB6504.seq Check: 3402 from: 1 to: 4259
pF1KB6504 4259 bp
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AccI GT'mk_AC
Cuts at: 1297 1297
Size: 4259
Acc65I G'GTAC_C
Cuts at: 1113 1275 1113
Size: 162 4097
AclI AA'CG_TT
Cuts at: 4099 4099
Size: 4259
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 811 2084 2516 3263 3531 811
Size: 1273 432 747 268 1539
Fragments arranged by size:
1539 1273 747 432 268
AflIII A'CryG_T
Cuts at: 1611 2715 3692 1611
Size: 1104 977 2178
Fragments arranged by size:
2178 1104 977
AgeI A'CCGG_T
Cuts at: 2647 2647
Size: 4259
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 415 1181 415
Size: 766 3493
AlwNI CAG_nnn'CTG
Cuts at: 1564 3131 1564
Size: 1567 2692
ApaLI G'TGCA_C
Cuts at: 3029 3029
Size: 4259
ApoI r'AATT_y
Cuts at: 1263 3612 3868 1263
Size: 2349 256 1654
Fragments arranged by size:
2349 1654 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4259
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4259
AvaI C'yCGr_G
Cuts at: 426 435 1057 1279 426
Size: 9 622 222 3406
Fragments arranged by size:
3406 622 222 9
BamHI G'GATC_C
Cuts at: 372 510 881 1284 372
Size: 138 371 403 3347
Fragments arranged by size:
3347 403 371 138
BanI G'GyrC_C
Cuts at: 272 830 1113 1275 1689 1937 1972 272
Size: 558 283 162 414 248 35 2559
Fragments arranged by size:
2559 558 414 283 248 162 35
BanII G_rGCy'C
Cuts at: 244 899 927 1067 1253 1273 2303 244
Size: 655 28 140 186 20 1030 2200
Fragments arranged by size:
2200 1030 655 186 140 28 20
BbeI G_GCGC'C
Cuts at: 1693 1941 1693
Size: 248 4011
BbsI GAAGACnn'nnnn_
Cuts at: 1088 1088
Size: 4259
BbvCI CC'TCA_GC
Cuts at: 982 982
Size: 4259
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4225
BciVI GTATCCnnnnn_n'
Cuts at: 2157 2918 4259 2157
Size: 761 1341 2157
Fragments arranged by size:
2157 1341 761
BclI T'GATC_A
Cuts at: 1779 1779
Size: 4259
BglII A'GATC_T
Cuts at: 1774 3811 1774
Size: 2037 2222
BlpI GC'TnA_GC
Cuts at: 721 1371 721
Size: 650 3609
Bme1580I G_kGCm'C
Cuts at: 677 684 1884 1977 3033 677
Size: 7 1200 93 1056 1903
Fragments arranged by size:
1903 1200 1056 93 7
BmrI ACTGGGnnnn_n'
Cuts at: 1471 1552 1887 4031 1471
Size: 81 335 2144 1699
Fragments arranged by size:
2144 1699 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2626 2626
Size: 4259
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 408 484 712 736 408
Size: 76 228 24 3931
Fragments arranged by size:
3931 228 76 24
Bpu10I CC'TnA_GC
Cuts at: 982 982
Size: 4259
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1435 2314 2806 3104 1435
Size: 879 492 298 2590
Fragments arranged by size:
2590 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3963 3963
Size: 4259
BsaAI yAC'GTr
Cuts at: 501 1612 501
Size: 1111 3148
BsaBI GATnn'nnATC
Cuts at: 6 1773 3810 6
Size: 1767 2037 455
Fragments arranged by size:
2037 1767 455
BsaHI Gr'CG_yC
Cuts at: 1690 1938 2640 4136 1690
Size: 248 702 1496 1813
Fragments arranged by size:
1813 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1448 1969 2647 2921 3068 3479 1448
Size: 521 678 274 147 411 2228
Fragments arranged by size:
2228 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1106 1136 1106
Size: 30 4229
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 301 999 301
Size: 698 3561
BseYI C'CCAG_C
Cuts at: 144 297 839 1684 2442 3019 3515 144
Size: 153 542 845 758 577 496 888
Fragments arranged by size:
888 845 758 577 542 496 153
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 988 988
Size: 4259
BsiEI CG_ry'CG
Cuts at: 104 1801 1847 3055 104
Size: 1697 46 1208 1308
Fragments arranged by size:
1697 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 394 899 1124 1273 2051 2241 3033 394
Size: 505 225 149 778 190 792 1620
Fragments arranged by size:
1620 792 778 505 225 190 149
BsmI GAATG_Cn'
Cuts at: 1217 1217
Size: 4259
BsmBI CGTCTCn'nnnn_
Cuts at: 388 388
Size: 4259
Bsp1286I G_dGCh'C
Cuts at: 244 394 677 684 899 927 1067 1124 1253
Size: 150 283 7 215 28 140 57 129
Cuts at: 1253 1273 1884 1977 2051 2241 2303 3033 244
Size: 20 611 93 74 190 62 730 1470
Fragments arranged by size:
1470 730 611 283 215 190 150 140
129 93 74 62 57 28 20 7
BspEI T'CCGG_A
Cuts at: 3479 3479
Size: 4259
BspHI T'CATG_A
Cuts at: 3435 3435
Size: 4259
BspMI ACCTGCnnnn'nnnn_
Cuts at: 481 1025 1309 1523 1825 2206 481
Size: 544 284 214 302 381 2534
Fragments arranged by size:
2534 544 381 302 284 214
BsrBI CCG'CTC
Cuts at: 50 266 2551 2605 4089 4254 50
Size: 216 2285 54 1484 165 55
Fragments arranged by size:
2285 1484 216 165 55 54
BsrDI GCAATG_nn'
Cuts at: 561 2171 561
Size: 1610 2649
BsrFI r'CCGG_y
Cuts at: 2257 2438 2647 2257
Size: 181 209 3869
Fragments arranged by size:
3869 209 181
BssSI C'ACGA_G
Cuts at: 2530 2888 2530
Size: 358 3901
BstAPI GCAn_nnn'nTGC
Cuts at: 1636 1636
Size: 4259
BstBI TT'CG_AA
Cuts at: 2620 2620
Size: 4259
BstEII G'GTnAC_C
Cuts at: 750 750
Size: 4259
BstYI r'GATC_y
Cuts at: 1 372 510 881 1284 1751 1774 2109 2355
Size: 371 138 371 403 467 23 335 246
Cuts at: 2355 3367 3453 3465 3811 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 467 449 403 371 371 346 335
246 138 86 23 12
BtgI C'CryG_G
Cuts at: 123 199 236 659 1196 123
Size: 76 37 423 537 3186
Fragments arranged by size:
3186 537 423 76 37
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2209 2390 2553 2209
Size: 181 163 3915
Fragments arranged by size:
3915 181 163
BtsI GCAGTG_nn'
Cuts at: 1502 1502
Size: 4259
DraI TTT'AAA
Cuts at: 1258 1258
Size: 4259
DrdI GACnn_nn'nnGTC
Cuts at: 1177 1965 2823 1177
Size: 788 858 2613
Fragments arranged by size:
2613 858 788
EaeI y'GGCC_r
Cuts at: 233 563 1844 2018 2409 2436 3781 233
Size: 330 1281 174 391 27 1345 711
Fragments arranged by size:
1345 1281 711 391 330 174 27
EagI C'GGCC_G
Cuts at: 1844 1844
Size: 4259
EarI CTCTTCn'nnn_
Cuts at: 631 772 776 2282 2492 631
Size: 141 4 1506 210 2398
Fragments arranged by size:
2398 1506 210 141 4
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2467 2777 2923 4069 2467
Size: 310 146 1146 2657
Fragments arranged by size:
2657 1146 310 146
EcoICRI GAG'CTC
Cuts at: 897 1271 897
Size: 374 3885
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 408 484 712 736 811 2084 2516 3263 3531
Size: 76 228 24 75 1273 432 747 268
Cuts at: 3531 408
Size: 1136
Fragments arranged by size:
1273 1136 747 432 268 228 76 75
24
EcoO109I rG'GnC_Cy
Cuts at: 315 802 1398 315
Size: 487 596 3176
Fragments arranged by size:
3176 596 487
EcoRI G'AATT_C
Cuts at: 1263 1263
Size: 4259
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2278 2278
Size: 4259
FspI TGC'GCA
Cuts at: 2040 2040
Size: 4259
HaeII r_GCGC'y
Cuts at: 189 232 790 1693 1941 2963 3942 189
Size: 43 558 903 248 1022 979 506
Fragments arranged by size:
1022 979 903 558 506 248 43
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 381 413 2101 2131 2133 2163 381
Size: 32 1688 30 2 30 2477
Fragments arranged by size:
2477 1688 32 30 30 2
HincII GTy'rAC
Cuts at: 1298 1298
Size: 4259
KasI G'GCGC_C
Cuts at: 1689 1937 1689
Size: 248 4011
KpnI G_GTAC'C
Cuts at: 1117 1279 1117
Size: 162 4097
MluI A'CGCG_T
Cuts at: 3692 3692
Size: 4259
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1077 2930 3114 1077
Size: 1853 184 2222
Fragments arranged by size:
2222 1853 184
MscI TGG'CCA
Cuts at: 565 2020 565
Size: 1455 2804
MslI CAynn'nnrTG
Cuts at: 2248 2375 2248
Size: 127 4132
MspA1I CmG'CkG
Cuts at: 125 201 297 968 1370 1684 2044 3057 3302
Size: 76 96 671 402 314 360 1013 245
Cuts at: 3302 125
Size: 1082
Fragments arranged by size:
1082 1013 671 402 360 314 245 96
76
NaeI GCC'GGC
Cuts at: 2440 2440
Size: 4259
NarI GG'CG_CC
Cuts at: 1690 1938 1690
Size: 248 4011
NgoMIV G'CCGG_C
Cuts at: 2438 2438
Size: 4259
NspI r_CATG'y
Cuts at: 1312 1812 2719 1312
Size: 500 907 2852
Fragments arranged by size:
2852 907 500
PasI CC'CwG_GG
Cuts at: 534 534
Size: 4259
PciI A'CATG_T
Cuts at: 2715 2715
Size: 4259
PflMI CCAn_nnn'nTGG
Cuts at: 846 846
Size: 4259
PfoI T'CCnGG_A
Cuts at: 461 461
Size: 4259
PmeI GTTT'AAAC
Cuts at: 1258 1258
Size: 4259
PmlI CAC'GTG
Cuts at: 501 501
Size: 4259
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 143 175 3398 3430 143
Size: 32 3223 32 972
Fragments arranged by size:
3223 972 32 32
PpuMI rG'GwC_Cy
Cuts at: 315 802 315
Size: 487 3772
PstI C_TGCA'G
Cuts at: 1025 1306 1991 1025
Size: 281 685 3293
Fragments arranged by size:
3293 685 281
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4259
PvuII CAG'CTG
Cuts at: 1684 2044 1684
Size: 360 3899
RsrII CG'GwC_CG
Cuts at: 963 2454 963
Size: 1491 2768
SacI G_AGCT'C
Cuts at: 899 1273 899
Size: 374 3885
SacII CC_GC'GG
Cuts at: 126 202 126
Size: 76 4183
SalI G'TCGA_C
Cuts at: 1296 1296
Size: 4259
SanDI GG'GwC_CC
Cuts at: 315 802 315
Size: 487 3772
SapI GCTCTTCn'nnn_
Cuts at: 631 772 2282 2492 631
Size: 141 1510 210 2398
Fragments arranged by size:
2398 1510 210 141
SbfI CC_TGCA'GG
Cuts at: 1306 1306
Size: 4259
ScaI AGT'ACT
Cuts at: 3776 3776
Size: 4259
SfcI C'TryA_G
Cuts at: 33 942 1021 1302 1987 2980 3171 33
Size: 909 79 281 685 993 191 1121
Fragments arranged by size:
1121 993 909 685 281 191 79
SfoI GGC'GCC
Cuts at: 1691 1939 1691
Size: 248 4011
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4259
SmaI CCC'GGG
Cuts at: 428 437 1059 1281 428
Size: 9 622 222 3406
Fragments arranged by size:
3406 622 222 9
SmlI C'TyrA_G
Cuts at: 1414 2329 2821 3083 1414
Size: 915 492 262 2590
Fragments arranged by size:
2590 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4259
SphI G_CATG'C
Cuts at: 1312 1812 1312
Size: 500 3759
StyI C'CwwG_G
Cuts at: 577 806 1393 1747 577
Size: 229 587 354 3089
Fragments arranged by size:
3089 587 354 229
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1205 1209 1842 1975 2434 2643 1205
Size: 4 633 133 459 209 2821
Fragments arranged by size:
2821 633 459 209 133 4
TatI w'GTAC_w
Cuts at: 1046 3774 1046
Size: 2728 1531
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3776 3808 3776
Size: 32 4227
Tth111I GACn'n_nGTC
Cuts at: 2056 2056
Size: 4259
XbaI T'CTAG_A
Cuts at: 1290 1290
Size: 4259
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 383 1754 383
Size: 1371 2888
XmaI C'CCGG_G
Cuts at: 426 435 1057 1279 426
Size: 9 622 222 3406
Fragments arranged by size:
3406 622 222 9
XmnI GAAnn'nnTTC
Cuts at: 625 625
Size: 4259
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BfrBI BglI BmgBI BmtI BsiWI
BsrGI BssHII BstXI BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NdeI NheI NotI NruI NsiI PacI PshAI PsiI
PspOMI PspXI PsrI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI ZraI