Restriction map for
pF1KB6505
(Circular) (Six-base) MAPSORT of: pF1KB6505.seq Check: 4748 from: 1 to: 4187
pF1KB6505 4187 bp
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AccI GT'mk_AC
Cuts at: 388 1225 388
Size: 837 3350
Acc65I G'GTAC_C
Cuts at: 493 1203 493
Size: 710 3477
AclI AA'CG_TT
Cuts at: 4027 4027
Size: 4187
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2012 2444 3191 3459 2012
Size: 432 747 268 2740
Fragments arranged by size:
2740 747 432 268
AflIII A'CryG_T
Cuts at: 767 1539 2643 3620 767
Size: 772 1104 977 1334
Fragments arranged by size:
1334 1104 977 772
AgeI A'CCGG_T
Cuts at: 2575 2575
Size: 4187
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1054 1054
Size: 4187
AlwNI CAG_nnn'CTG
Cuts at: 363 382 1492 3059 363
Size: 19 1110 1567 1491
Fragments arranged by size:
1567 1491 1110 19
ApaI G_GGCC'C
Cuts at: 224 317 224
Size: 93 4094
ApaLI G'TGCA_C
Cuts at: 2957 2957
Size: 4187
ApoI r'AATT_y
Cuts at: 1191 3540 3796 1191
Size: 2349 256 1582
Fragments arranged by size:
2349 1582 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4187
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4187
AvaI C'yCGr_G
Cuts at: 334 819 1207 334
Size: 485 388 3314
Fragments arranged by size:
3314 485 388
BamHI G'GATC_C
Cuts at: 1212 1212
Size: 4187
BanI G'GyrC_C
Cuts at: 493 1203 1617 1865 1900 493
Size: 710 414 248 35 2780
Fragments arranged by size:
2780 710 414 248 35
BanII G_rGCy'C
Cuts at: 213 224 262 317 335 437 1201 2231 213
Size: 11 38 55 18 102 764 1030 2169
Fragments arranged by size:
2169 1030 764 102 55 38 18 11
BbeI G_GCGC'C
Cuts at: 1621 1869 1621
Size: 248 3939
BbsI GAAGACnn'nnnn_
Cuts at: 666 666
Size: 4187
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 451 485 941 975 90
Size: 34 327 34 456 34 3302
Fragments arranged by size:
3302 456 327 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 577 2085 2846 4187 577
Size: 1508 761 1341 577
Fragments arranged by size:
1508 1341 761 577
BclI T'GATC_A
Cuts at: 1707 1707
Size: 4187
BglI GCCn_nnn'nGGC
Cuts at: 358 377 358
Size: 19 4168
BglII A'GATC_T
Cuts at: 1702 3739 1702
Size: 2037 2150
BlpI GC'TnA_GC
Cuts at: 1299 1299
Size: 4187
Bme1580I G_kGCm'C
Cuts at: 224 317 775 1812 1905 2961 224
Size: 93 458 1037 93 1056 1450
Fragments arranged by size:
1450 1056 1037 458 93 93
BmrI ACTGGGnnnn_n'
Cuts at: 746 1135 1399 1480 1815 3959 746
Size: 389 264 81 335 2144 974
Fragments arranged by size:
2144 974 389 335 264 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2554 2554
Size: 4187
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 257 349 451 727 736 861 257
Size: 92 102 276 9 125 3583
Fragments arranged by size:
3583 276 125 102 92 9
Bpu10I CC'TnA_GC
Cuts at: 256 572 1071 256
Size: 316 499 3372
Fragments arranged by size:
3372 499 316
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 640 1363 2242 2734 3032 640
Size: 723 879 492 298 1795
Fragments arranged by size:
1795 879 723 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3891 3891
Size: 4187
BsaAI yAC'GTr
Cuts at: 783 1540 783
Size: 757 3430
BsaBI GATnn'nnATC
Cuts at: 6 1701 3738 6
Size: 1695 2037 455
Fragments arranged by size:
2037 1695 455
BsaHI Gr'CG_yC
Cuts at: 1618 1866 2568 4064 1618
Size: 248 702 1496 1741
Fragments arranged by size:
1741 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1376 1897 2575 2849 2996 3407 1376
Size: 521 678 274 147 411 2156
Fragments arranged by size:
2156 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 352 493 1165 352
Size: 141 672 3374
Fragments arranged by size:
3374 672 141
BseYI C'CCAG_C
Cuts at: 224 497 774 1612 2370 2947 3443 224
Size: 273 277 838 758 577 496 968
Fragments arranged by size:
968 838 758 577 496 277 273
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 349 1024 349
Size: 675 3512
BsiEI CG_ry'CG
Cuts at: 104 1729 1775 2983 104
Size: 1625 46 1208 1308
Fragments arranged by size:
1625 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 213 593 1201 1979 2169 2961 213
Size: 380 608 778 190 792 1439
Fragments arranged by size:
1439 792 778 608 380 190
Bsp1286I G_dGCh'C
Cuts at: 213 224 262 317 335 437 593 775 1201
Size: 11 38 55 18 102 156 182 426
Cuts at: 1201 1812 1905 1979 2169 2231 2961 213
Size: 611 93 74 190 62 730 1439
Fragments arranged by size:
1439 730 611 426 190 182 156 102
93 74 62 55 38 18 11
BspEI T'CCGG_A
Cuts at: 3407 3407
Size: 4187
BspHI T'CATG_A
Cuts at: 3363 3363
Size: 4187
BspMI ACCTGCnnnn'nnnn_
Cuts at: 400 1237 1451 1753 2134 400
Size: 837 214 302 381 2453
Fragments arranged by size:
2453 837 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2479 2533 4017 4182 50
Size: 2429 54 1484 165 55
Fragments arranged by size:
2429 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2099 2099
Size: 4187
BsrFI r'CCGG_y
Cuts at: 874 2185 2366 2575 874
Size: 1311 181 209 2486
Fragments arranged by size:
2486 1311 209 181
BssSI C'ACGA_G
Cuts at: 2458 2816 2458
Size: 358 3829
BstAPI GCAn_nnn'nTGC
Cuts at: 1564 1564
Size: 4187
BstBI TT'CG_AA
Cuts at: 2548 2548
Size: 4187
BstXI CCAn_nnnn'nTGG
Cuts at: 123 615 123
Size: 492 3695
BstYI r'GATC_y
Cuts at: 1 923 1212 1679 1702 2037 2283 3295 3381
Size: 922 289 467 23 335 246 1012 86
Cuts at: 3381 3393 3739 1
Size: 12 346 449
Fragments arranged by size:
1012 922 467 449 346 335 289 246
86 23 12
BstZ17I GTA'TAC
Cuts at: 389 389
Size: 4187
Bsu36I CC'TnA_GG
Cuts at: 578 578
Size: 4187
BtgI C'CryG_G
Cuts at: 528 930 528
Size: 402 3785
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2137 2318 2481 2137
Size: 181 163 3843
Fragments arranged by size:
3843 181 163
BtsI GCAGTG_nn'
Cuts at: 296 366 1430 296
Size: 70 1064 3053
Fragments arranged by size:
3053 1064 70
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 272 307 272
Size: 35 4152
DraI TTT'AAA
Cuts at: 1186 1186
Size: 4187
DrdI GACnn_nn'nnGTC
Cuts at: 1893 2751 1893
Size: 858 3329
EaeI y'GGCC_r
Cuts at: 293 422 605 872 876 1772 1946 2337 2364
Size: 129 183 267 4 896 174 391 27
Cuts at: 2364 3709 293
Size: 1345 771
Fragments arranged by size:
1345 896 771 391 267 183 174 129
27 4
EagI C'GGCC_G
Cuts at: 1772 1772
Size: 4187
EarI CTCTTCn'nnn_
Cuts at: 598 865 919 1142 2210 2420 598
Size: 267 54 223 1068 210 2365
Fragments arranged by size:
2365 1068 267 223 210 54
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 586 916 2395 2705 2851 3997 586
Size: 330 1479 310 146 1146 776
Fragments arranged by size:
1479 1146 776 330 310 146
EcoICRI GAG'CTC
Cuts at: 211 1199 211
Size: 988 3199
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 257 349 451 727 736 861 2012 2444 3191
Size: 92 102 276 9 125 1151 432 747
Cuts at: 3191 3459 257
Size: 268 985
Fragments arranged by size:
1151 985 747 432 276 268 125 102
92 9
EcoO109I rG'GnC_Cy
Cuts at: 221 314 360 483 787 935 1059 1326 221
Size: 93 46 123 304 148 124 267 3082
Fragments arranged by size:
3082 304 267 148 124 123 93 46
EcoRI G'AATT_C
Cuts at: 1191 1191
Size: 4187
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2206 2206
Size: 4187
FseI GG_CCGG'CC
Cuts at: 878 878
Size: 4187
FspI TGC'GCA
Cuts at: 1968 1968
Size: 4187
HaeII r_GCGC'y
Cuts at: 859 1621 1869 2891 3870 859
Size: 762 248 1022 979 1176
Fragments arranged by size:
1176 1022 979 762 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1076 1108 2029 2059 2061 2091 1076
Size: 32 921 30 2 30 3172
Fragments arranged by size:
3172 921 32 30 30 2
HincII GTy'rAC
Cuts at: 1226 1226
Size: 4187
KasI G'GCGC_C
Cuts at: 1617 1865 1617
Size: 248 3939
KpnI G_GTAC'C
Cuts at: 497 1207 497
Size: 710 3477
MluI A'CGCG_T
Cuts at: 767 3620 767
Size: 2853 1334
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 143 149 2858 3042 143
Size: 6 2709 184 1288
Fragments arranged by size:
2709 1288 184 6
MscI TGG'CCA
Cuts at: 295 607 1948 295
Size: 312 1341 2534
Fragments arranged by size:
2534 1341 312
MslI CAynn'nnrTG
Cuts at: 461 2176 2303 461
Size: 1715 127 2345
Fragments arranged by size:
2345 1715 127
MspA1I CmG'CkG
Cuts at: 932 974 1009 1298 1612 1972 2985 3230 932
Size: 42 35 289 314 360 1013 245 1889
Fragments arranged by size:
1889 1013 360 314 289 245 42 35
NaeI GCC'GGC
Cuts at: 876 2368 876
Size: 1492 2695
NarI GG'CG_CC
Cuts at: 1618 1866 1618
Size: 248 3939
NcoI C'CATG_G
Cuts at: 528 528
Size: 4187
NgoMIV G'CCGG_C
Cuts at: 874 2366 874
Size: 1492 2695
NspI r_CATG'y
Cuts at: 466 540 1240 1740 2647 466
Size: 74 700 500 907 2006
Fragments arranged by size:
2006 907 700 500 74
PciI A'CATG_T
Cuts at: 2643 2643
Size: 4187
PfoI T'CCnGG_A
Cuts at: 148 326 410 890 148
Size: 178 84 480 3445
Fragments arranged by size:
3445 480 178 84
PmeI GTTT'AAAC
Cuts at: 1186 1186
Size: 4187
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1154 1186 3326 3358 1154
Size: 32 2140 32 1983
Fragments arranged by size:
2140 1983 32 32
PpuMI rG'GwC_Cy
Cuts at: 483 787 1059 483
Size: 304 272 3611
Fragments arranged by size:
3611 304 272
PspOMI G'GGCC_C
Cuts at: 220 313 220
Size: 93 4094
PstI C_TGCA'G
Cuts at: 954 1234 1919 954
Size: 280 685 3222
Fragments arranged by size:
3222 685 280
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4187
PvuII CAG'CTG
Cuts at: 1009 1612 1972 1009
Size: 603 360 3224
Fragments arranged by size:
3224 603 360
RsrII CG'GwC_CG
Cuts at: 2382 2382
Size: 4187
SacI G_AGCT'C
Cuts at: 213 1201 213
Size: 988 3199
SacII CC_GC'GG
Cuts at: 933 933
Size: 4187
SalI G'TCGA_C
Cuts at: 1224 1224
Size: 4187
SapI GCTCTTCn'nnn_
Cuts at: 598 865 919 2210 2420 598
Size: 267 54 1291 210 2365
Fragments arranged by size:
2365 1291 267 210 54
SbfI CC_TGCA'GG
Cuts at: 954 1234 954
Size: 280 3907
ScaI AGT'ACT
Cuts at: 3704 3704
Size: 4187
SfcI C'TryA_G
Cuts at: 33 374 950 1230 1915 2908 3099 33
Size: 341 576 280 685 993 191 1121
Fragments arranged by size:
1121 993 685 576 341 280 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 358 358
Size: 4187
SfoI GGC'GCC
Cuts at: 1619 1867 1619
Size: 248 3939
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4187
SmaI CCC'GGG
Cuts at: 1209 1209
Size: 4187
SmlI C'TyrA_G
Cuts at: 655 1342 2257 2749 3011 655
Size: 687 915 492 262 1831
Fragments arranged by size:
1831 915 687 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4187
SphI G_CATG'C
Cuts at: 1240 1740 1240
Size: 500 3687
StuI AGG'CCT
Cuts at: 956 956
Size: 4187
StyI C'CwwG_G
Cuts at: 528 1321 1675 528
Size: 793 354 3040
Fragments arranged by size:
3040 793 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1770 1903 2362 2571 1770
Size: 133 459 209 3386
Fragments arranged by size:
3386 459 209 133
TatI w'GTAC_w
Cuts at: 3702 3702
Size: 4187
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3704 3736 3704
Size: 32 4155
Tth111I GACn'n_nGTC
Cuts at: 1984 1984
Size: 4187
XbaI T'CTAG_A
Cuts at: 1218 1218
Size: 4187
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 483 1682 483
Size: 1199 2988
XmaI C'CCGG_G
Cuts at: 1207 1207
Size: 4187
XmnI GAAnn'nnTTC
Cuts at: 1169 1169
Size: 4187
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FseI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PfoI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI AscI
AvrII BaeI BbvCI BfrBI BmgBI BmtI BsaXI BsiWI
BsmI BsmBI BsrGI BssHII BstEII ClaI DraIII EcoNI
EcoRV FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PmlI PshAI PsiI
PspXI PsrI SanDI SexAI SgrAI SnaBI SrfI SspI
SwaI XhoI ZraI