Restriction map for
pF1KB6507
(Circular) (Six-base) MAPSORT of: pF1KB6507.seq Check: 3106 from: 1 to: 4190
pF1KB6507 4190 bp
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AccI GT'mk_AC
Cuts at: 344 1228 344
Size: 884 3306
Acc65I G'GTAC_C
Cuts at: 1206 1206
Size: 4190
AclI AA'CG_TT
Cuts at: 4030 4030
Size: 4190
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1057 2015 2447 3194 3462 1057
Size: 958 432 747 268 1785
Fragments arranged by size:
1785 958 747 432 268
AflIII A'CryG_T
Cuts at: 1093 1542 2646 3623 1093
Size: 449 1104 977 1660
Fragments arranged by size:
1660 1104 977 449
AgeI A'CCGG_T
Cuts at: 2578 2578
Size: 4190
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 763 795 763
Size: 32 4158
AlwNI CAG_nnn'CTG
Cuts at: 1495 3062 1495
Size: 1567 2623
ApaLI G'TGCA_C
Cuts at: 2960 2960
Size: 4190
ApoI r'AATT_y
Cuts at: 1194 3543 3799 1194
Size: 2349 256 1585
Fragments arranged by size:
2349 1585 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4190
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4190
AvaI C'yCGr_G
Cuts at: 522 985 1210 522
Size: 463 225 3502
Fragments arranged by size:
3502 463 225
BamHI G'GATC_C
Cuts at: 755 1215 755
Size: 460 3730
BanI G'GyrC_C
Cuts at: 298 1206 1620 1868 1903 298
Size: 908 414 248 35 2585
Fragments arranged by size:
2585 908 414 248 35
BanII G_rGCy'C
Cuts at: 227 1204 2234 227
Size: 977 1030 2183
Fragments arranged by size:
2183 1030 977
BbeI G_GCGC'C
Cuts at: 302 1624 1872 302
Size: 1322 248 2620
Fragments arranged by size:
2620 1322 248
BbvCI CC'TCA_GC
Cuts at: 802 802
Size: 4190
BciVI GTATCCnnnnn_n'
Cuts at: 2088 2849 4190 2088
Size: 761 1341 2088
Fragments arranged by size:
2088 1341 761
BclI T'GATC_A
Cuts at: 1710 1710
Size: 4190
BglI GCCn_nnn'nGGC
Cuts at: 370 1018 370
Size: 648 3542
BglII A'GATC_T
Cuts at: 1705 3742 1705
Size: 2037 2153
BlpI GC'TnA_GC
Cuts at: 1302 1302
Size: 4190
Bme1580I G_kGCm'C
Cuts at: 662 1815 1908 2964 662
Size: 1153 93 1056 1888
Fragments arranged by size:
1888 1153 1056 93
BmgBI CAC'GTC
Cuts at: 459 642 459
Size: 183 4007
BmrI ACTGGGnnnn_n'
Cuts at: 1402 1483 1818 3962 1402
Size: 81 335 2144 1630
Fragments arranged by size:
2144 1630 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2557 2557
Size: 4190
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 136 715 964 136
Size: 579 249 3362
Fragments arranged by size:
3362 579 249
Bpu10I CC'TnA_GC
Cuts at: 139 802 139
Size: 663 3527
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 211 382 1150 1366 2245 2737 3035 211
Size: 171 768 216 879 492 298 1366
Fragments arranged by size:
1366 879 768 492 298 216 171
BsaI GGTCTCn'nnnn_
Cuts at: 3894 3894
Size: 4190
BsaAI yAC'GTr
Cuts at: 1094 1543 1094
Size: 449 3741
BsaBI GATnn'nnATC
Cuts at: 6 286 1704 3741 6
Size: 280 1418 2037 455
Fragments arranged by size:
2037 1418 455 280
BsaHI Gr'CG_yC
Cuts at: 212 299 1010 1621 1869 2571 4067 212
Size: 87 711 611 248 702 1496 335
Fragments arranged by size:
1496 711 702 611 335 248 87
BsaWI w'CCGG_w
Cuts at: 710 845 872 1379 1900 2578 2852 2999 3410
Size: 135 27 507 521 678 274 147 411
Cuts at: 3410 710
Size: 1490
Fragments arranged by size:
1490 678 521 507 411 274 147 135
27
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 550 580 550
Size: 30 4160
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 328 868 897 328
Size: 540 29 3621
Fragments arranged by size:
3621 540 29
BseYI C'CCAG_C
Cuts at: 632 1001 1615 2373 2950 3446 632
Size: 369 614 758 577 496 1376
Fragments arranged by size:
1376 758 614 577 496 369
BsiEI CG_ry'CG
Cuts at: 104 546 739 1136 1732 1778 2986 104
Size: 442 193 397 596 46 1208 1308
Fragments arranged by size:
1308 1208 596 442 397 193 46
BsiHKAI G_wGCw'C
Cuts at: 227 268 1204 1982 2172 2964 227
Size: 41 936 778 190 792 1453
Fragments arranged by size:
1453 936 792 778 190 41
BsmI GAATG_Cn'
Cuts at: 541 541
Size: 4190
BsmBI CGTCTCn'nnnn_
Cuts at: 385 385
Size: 4190
Bsp1286I G_dGCh'C
Cuts at: 227 268 662 1204 1815 1908 1982 2172 2234
Size: 41 394 542 611 93 74 190 62
Cuts at: 2234 2964 227
Size: 730 1453
Fragments arranged by size:
1453 730 611 542 394 190 93 74
62 41
BspEI T'CCGG_A
Cuts at: 710 872 3410 710
Size: 162 2538 1490
Fragments arranged by size:
2538 1490 162
BspHI T'CATG_A
Cuts at: 3366 3366
Size: 4190
BspMI ACCTGCnnnn'nnnn_
Cuts at: 632 947 1240 1454 1756 2137 632
Size: 315 293 214 302 381 2685
Fragments arranged by size:
2685 381 315 302 293 214
BsrBI CCG'CTC
Cuts at: 50 257 744 2482 2536 4020 4185 50
Size: 207 487 1738 54 1484 165 55
Fragments arranged by size:
1738 1484 487 207 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2102 2102
Size: 4190
BsrFI r'CCGG_y
Cuts at: 301 667 2188 2369 2578 301
Size: 366 1521 181 209 1913
Fragments arranged by size:
1913 1521 366 209 181
BssSI C'ACGA_G
Cuts at: 2461 2819 2461
Size: 358 3832
BstAPI GCAn_nnn'nTGC
Cuts at: 642 1567 642
Size: 925 3265
BstBI TT'CG_AA
Cuts at: 2551 2551
Size: 4190
BstEII G'GTnAC_C
Cuts at: 468 468
Size: 4190
BstXI CCAn_nnnn'nTGG
Cuts at: 114 114
Size: 4190
BstYI r'GATC_y
Cuts at: 1 401 755 953 1215 1682 1705 2040 2286
Size: 400 354 198 262 467 23 335 246
Cuts at: 2286 3298 3384 3396 3742 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 467 449 400 354 346 335 262
246 198 86 23 12
BtgI C'CryG_G
Cuts at: 294 386 294
Size: 92 4098
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 158 591 2140 2321 2484 158
Size: 433 1549 181 163 1864
Fragments arranged by size:
1864 1549 433 181 163
BtsI GCAGTG_nn'
Cuts at: 759 1433 759
Size: 674 3516
DraI TTT'AAA
Cuts at: 1189 1189
Size: 4190
DraIII CAC_nnn'GTG
Cuts at: 972 972
Size: 4190
DrdI GACnn_nn'nnGTC
Cuts at: 117 1896 2754 117
Size: 1779 858 1553
Fragments arranged by size:
1779 1553 858
EaeI y'GGCC_r
Cuts at: 1133 1775 1949 2340 2367 3712 1133
Size: 642 174 391 27 1345 1611
Fragments arranged by size:
1611 1345 642 391 174 27
EagI C'GGCC_G
Cuts at: 1133 1775 1133
Size: 642 3548
EarI CTCTTCn'nnn_
Cuts at: 256 925 2213 2423 256
Size: 669 1288 210 2023
Fragments arranged by size:
2023 1288 669 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2398 2708 2854 4000 2398
Size: 310 146 1146 2588
Fragments arranged by size:
2588 1146 310 146
EcoICRI GAG'CTC
Cuts at: 225 1202 225
Size: 977 3213
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 136 715 964 1057 2015 2447 3194 3462 136
Size: 579 249 93 958 432 747 268 864
Fragments arranged by size:
958 864 747 579 432 268 249 93
EcoNI CCTnn'n_nnAGG
Cuts at: 514 943 514
Size: 429 3761
EcoO109I rG'GnC_Cy
Cuts at: 519 936 1329 519
Size: 417 393 3380
Fragments arranged by size:
3380 417 393
EcoRI G'AATT_C
Cuts at: 1194 1194
Size: 4190
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 345 2209 345
Size: 1864 2326
FspI TGC'GCA
Cuts at: 286 1971 286
Size: 1685 2505
FspAI rTGC'GCAy
Cuts at: 286 286
Size: 4190
HaeII r_GCGC'y
Cuts at: 302 1624 1872 2894 3873 302
Size: 1322 248 1022 979 619
Fragments arranged by size:
1322 1022 979 619 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2032 2062 2064 2094 2032
Size: 30 2 30 4128
Fragments arranged by size:
4128 30 30 2
HincII GTy'rAC
Cuts at: 600 1229 600
Size: 629 3561
KasI G'GCGC_C
Cuts at: 298 1620 1868 298
Size: 1322 248 2620
Fragments arranged by size:
2620 1322 248
KpnI G_GTAC'C
Cuts at: 1210 1210
Size: 4190
MluI A'CGCG_T
Cuts at: 3623 3623
Size: 4190
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 593 719 812 2861 3045 593
Size: 126 93 2049 184 1738
Fragments arranged by size:
2049 1738 184 126 93
MscI TGG'CCA
Cuts at: 1951 1951
Size: 4190
MslI CAynn'nnrTG
Cuts at: 364 2179 2306 364
Size: 1815 127 2248
Fragments arranged by size:
2248 1815 127
MspA1I CmG'CkG
Cuts at: 192 735 1005 1301 1615 1975 2988 3233 192
Size: 543 270 296 314 360 1013 245 1149
Fragments arranged by size:
1149 1013 543 360 314 296 270 245
NaeI GCC'GGC
Cuts at: 303 2371 303
Size: 2068 2122
NarI GG'CG_CC
Cuts at: 299 1621 1869 299
Size: 1322 248 2620
Fragments arranged by size:
2620 1322 248
NcoI C'CATG_G
Cuts at: 386 386
Size: 4190
NgoMIV G'CCGG_C
Cuts at: 301 2369 301
Size: 2068 2122
NspI r_CATG'y
Cuts at: 1243 1743 2650 1243
Size: 500 907 2783
Fragments arranged by size:
2783 907 500
PciI A'CATG_T
Cuts at: 2646 2646
Size: 4190
PflMI CCAn_nnn'nTGG
Cuts at: 822 822
Size: 4190
PfoI T'CCnGG_A
Cuts at: 446 530 446
Size: 84 4106
PmeI GTTT'AAAC
Cuts at: 1189 1189
Size: 4190
PmlI CAC'GTG
Cuts at: 1094 1094
Size: 4190
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3329 3361 3329
Size: 32 4158
PpuMI rG'GwC_Cy
Cuts at: 519 936 519
Size: 417 3773
PstI C_TGCA'G
Cuts at: 1157 1237 1922 1157
Size: 80 685 3425
Fragments arranged by size:
3425 685 80
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4190
PvuII CAG'CTG
Cuts at: 192 1615 1975 192
Size: 1423 360 2407
Fragments arranged by size:
2407 1423 360
RsrII CG'GwC_CG
Cuts at: 2385 2385
Size: 4190
SacI G_AGCT'C
Cuts at: 227 1204 227
Size: 977 3213
SalI G'TCGA_C
Cuts at: 1227 1227
Size: 4190
SapI GCTCTTCn'nnn_
Cuts at: 256 2213 2423 256
Size: 1957 210 2023
Fragments arranged by size:
2023 1957 210
SbfI CC_TGCA'GG
Cuts at: 1237 1237
Size: 4190
ScaI AGT'ACT
Cuts at: 583 3707 583
Size: 3124 1066
SexAI A'CCwGG_T
Cuts at: 968 1178 968
Size: 210 3980
SfcI C'TryA_G
Cuts at: 33 1153 1233 1918 2911 3102 33
Size: 1120 80 685 993 191 1121
Fragments arranged by size:
1121 1120 993 685 191 80
SfoI GGC'GCC
Cuts at: 300 1622 1870 300
Size: 1322 248 2620
Fragments arranged by size:
2620 1322 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4190
SmaI CCC'GGG
Cuts at: 524 1212 524
Size: 688 3502
SmlI C'TyrA_G
Cuts at: 226 397 1165 1345 2260 2752 3014 226
Size: 171 768 180 915 492 262 1402
Fragments arranged by size:
1402 915 768 492 262 180 171
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4190
SphI G_CATG'C
Cuts at: 1243 1743 1243
Size: 500 3690
StyI C'CwwG_G
Cuts at: 386 614 1324 1678 386
Size: 228 710 354 2898
Fragments arranged by size:
2898 710 354 228
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 607 646 1773 1906 2365 2574 607
Size: 39 1127 133 459 209 2223
Fragments arranged by size:
2223 1127 459 209 133 39
TatI w'GTAC_w
Cuts at: 581 959 1172 3705 581
Size: 378 213 2533 1066
Fragments arranged by size:
2533 1066 378 213
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3707 3739 3707
Size: 32 4158
Tth111I GACn'n_nGTC
Cuts at: 817 1987 817
Size: 1170 3020
XbaI T'CTAG_A
Cuts at: 1221 1221
Size: 4190
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1685 1685
Size: 4190
XmaI C'CCGG_G
Cuts at: 522 1210 522
Size: 688 3502
XmnI GAAnn'nnTTC
Cuts at: 355 1042 355
Size: 687 3503
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbvCI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
FspAI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI ApaI
AscI AvrII BaeI BbsI BcgI BfrBI BmtI BsgI
BsiWI BsrGI BssHII BstZ17I Bsu36I ClaI CspCI EcoRV
FseI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI ZraI