Restriction map for
pF1KB6509
(Circular) (Six-base) MAPSORT of: pF1KB6509.seq Check: 7812 from: 1 to: 4190
pF1KB6509 4190 bp
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AccI GT'mk_AC
Cuts at: 998 1148 1228 998
Size: 150 80 3960
Fragments arranged by size:
3960 150 80
Acc65I G'GTAC_C
Cuts at: 1206 1206
Size: 4190
AclI AA'CG_TT
Cuts at: 4030 4030
Size: 4190
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 439 547 550 685 1122 2015 2447 3194 3462
Size: 108 3 135 437 893 432 747 268
Cuts at: 3462 439
Size: 1167
Fragments arranged by size:
1167 893 747 437 432 268 135 108
3
AflIII A'CryG_T
Cuts at: 1542 2646 3623 1542
Size: 1104 977 2109
Fragments arranged by size:
2109 1104 977
AgeI A'CCGG_T
Cuts at: 2578 2578
Size: 4190
AleI CACnn'nnGTG
Cuts at: 598 598
Size: 4190
AlwNI CAG_nnn'CTG
Cuts at: 570 1495 3062 570
Size: 925 1567 1698
Fragments arranged by size:
1698 1567 925
ApaLI G'TGCA_C
Cuts at: 2960 2960
Size: 4190
ApoI r'AATT_y
Cuts at: 1194 3543 3799 1194
Size: 2349 256 1585
Fragments arranged by size:
2349 1585 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4190
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4190
AvaI C'yCGr_G
Cuts at: 1210 1210
Size: 4190
AvrII C'CTAG_G
Cuts at: 255 255
Size: 4190
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 941 974 941
Size: 33 4157
BamHI G'GATC_C
Cuts at: 1215 1215
Size: 4190
BanI G'GyrC_C
Cuts at: 652 1206 1620 1868 1903 652
Size: 554 414 248 35 2939
Fragments arranged by size:
2939 554 414 248 35
BanII G_rGCy'C
Cuts at: 251 673 1204 2234 251
Size: 422 531 1030 2207
Fragments arranged by size:
2207 1030 531 422
BbeI G_GCGC'C
Cuts at: 1624 1872 1624
Size: 248 3942
BbsI GAAGACnn'nnnn_
Cuts at: 754 754
Size: 4190
BciVI GTATCCnnnnn_n'
Cuts at: 2088 2849 4190 2088
Size: 761 1341 2088
Fragments arranged by size:
2088 1341 761
BclI T'GATC_A
Cuts at: 1710 1710
Size: 4190
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 4190
BglII A'GATC_T
Cuts at: 1705 3742 1705
Size: 2037 2153
BlpI GC'TnA_GC
Cuts at: 1302 1302
Size: 4190
Bme1580I G_kGCm'C
Cuts at: 271 579 655 1815 1908 2964 271
Size: 308 76 1160 93 1056 1497
Fragments arranged by size:
1497 1160 1056 308 93 76
BmrI ACTGGGnnnn_n'
Cuts at: 256 658 1125 1402 1483 1818 3962 256
Size: 402 467 277 81 335 2144 484
Fragments arranged by size:
2144 484 467 402 335 277 81
BmtI G_CTAG'C
Cuts at: 827 827
Size: 4190
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2557 2557
Size: 4190
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 494 494
Size: 4190
Bpu10I CC'TnA_GC
Cuts at: 672 672
Size: 4190
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 740 1366 2245 2737 3035 740
Size: 626 879 492 298 1895
Fragments arranged by size:
1895 879 626 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3894 3894
Size: 4190
BsaAI yAC'GTr
Cuts at: 1152 1543 1152
Size: 391 3799
BsaBI GATnn'nnATC
Cuts at: 6 1704 3741 6
Size: 1698 2037 455
Fragments arranged by size:
2037 1698 455
BsaHI Gr'CG_yC
Cuts at: 1621 1869 2571 4067 1621
Size: 248 702 1496 1744
Fragments arranged by size:
1744 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1379 1900 2578 2852 2999 3410 1379
Size: 521 678 274 147 411 2159
Fragments arranged by size:
2159 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1615 2373 2950 3446 1615
Size: 758 577 496 2359
Fragments arranged by size:
2359 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1732 1778 2986 104
Size: 1628 46 1208 1308
Fragments arranged by size:
1628 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 673 1204 1982 2172 2964 673
Size: 531 778 190 792 1899
Fragments arranged by size:
1899 792 778 531 190
BsmI GAATG_Cn'
Cuts at: 749 749
Size: 4190
Bsp1286I G_dGCh'C
Cuts at: 251 271 579 655 673 1204 1815 1908 1982
Size: 20 308 76 18 531 611 93 74
Cuts at: 1982 2172 2234 2964 251
Size: 190 62 730 1477
Fragments arranged by size:
1477 730 611 531 308 190 93 76
74 62 20 18
BspEI T'CCGG_A
Cuts at: 3410 3410
Size: 4190
BspHI T'CATG_A
Cuts at: 3366 3366
Size: 4190
BspMI ACCTGCnnnn'nnnn_
Cuts at: 377 1240 1454 1756 2137 377
Size: 863 214 302 381 2430
Fragments arranged by size:
2430 863 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2482 2536 4020 4185 50
Size: 2432 54 1484 165 55
Fragments arranged by size:
2432 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 725 2102 725
Size: 1377 2813
BsrFI r'CCGG_y
Cuts at: 2188 2369 2578 2188
Size: 181 209 3800
Fragments arranged by size:
3800 209 181
BssSI C'ACGA_G
Cuts at: 2461 2819 2461
Size: 358 3832
BstAPI GCAn_nnn'nTGC
Cuts at: 1567 1567
Size: 4190
BstBI TT'CG_AA
Cuts at: 2551 2551
Size: 4190
BstYI r'GATC_y
Cuts at: 1 339 1215 1682 1705 2040 2286 3298 3384
Size: 338 876 467 23 335 246 1012 86
Cuts at: 3384 3396 3742 1
Size: 12 346 449
Fragments arranged by size:
1012 876 467 449 346 338 335 246
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2140 2321 2484 2140
Size: 181 163 3846
Fragments arranged by size:
3846 181 163
BtsI GCAGTG_nn'
Cuts at: 1433 1433
Size: 4190
DraI TTT'AAA
Cuts at: 1189 1189
Size: 4190
DrdI GACnn_nn'nnGTC
Cuts at: 1896 2754 1896
Size: 858 3332
EaeI y'GGCC_r
Cuts at: 902 1775 1949 2340 2367 3712 902
Size: 873 174 391 27 1345 1380
Fragments arranged by size:
1380 1345 873 391 174 27
EagI C'GGCC_G
Cuts at: 1775 1775
Size: 4190
EarI CTCTTCn'nnn_
Cuts at: 566 661 2213 2423 566
Size: 95 1552 210 2333
Fragments arranged by size:
2333 1552 210 95
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2398 2708 2854 4000 2398
Size: 310 146 1146 2588
Fragments arranged by size:
2588 1146 310 146
EcoICRI GAG'CTC
Cuts at: 671 1202 671
Size: 531 3659
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 439 494 547 550 685 1122 2015 2447 3194
Size: 55 53 3 135 437 893 432 747
Cuts at: 3194 3462 439
Size: 268 1167
Fragments arranged by size:
1167 893 747 437 432 268 135 55
53 3
EcoO109I rG'GnC_Cy
Cuts at: 1329 1329
Size: 4190
EcoRI G'AATT_C
Cuts at: 1194 1194
Size: 4190
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2209 2209
Size: 4190
FspI TGC'GCA
Cuts at: 1971 1971
Size: 4190
HaeII r_GCGC'y
Cuts at: 1624 1872 2894 3873 1624
Size: 248 1022 979 1941
Fragments arranged by size:
1941 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 880 912 2032 2062 2064 2094 880
Size: 32 1120 30 2 30 2976
Fragments arranged by size:
2976 1120 32 30 30 2
HincII GTy'rAC
Cuts at: 447 888 1229 447
Size: 441 341 3408
Fragments arranged by size:
3408 441 341
HindIII A'AGCT_T
Cuts at: 946 946
Size: 4190
KasI G'GCGC_C
Cuts at: 1620 1868 1620
Size: 248 3942
KpnI G_GTAC'C
Cuts at: 1210 1210
Size: 4190
MluI A'CGCG_T
Cuts at: 3623 3623
Size: 4190
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 479 1008 2861 3045 479
Size: 529 1853 184 1624
Fragments arranged by size:
1853 1624 529 184
MscI TGG'CCA
Cuts at: 904 1951 904
Size: 1047 3143
MslI CAynn'nnrTG
Cuts at: 584 598 791 2179 2306 584
Size: 14 193 1388 127 2468
Fragments arranged by size:
2468 1388 193 127 14
MspA1I CmG'CkG
Cuts at: 278 528 663 1301 1615 1975 2988 3233 278
Size: 250 135 638 314 360 1013 245 1235
Fragments arranged by size:
1235 1013 638 360 314 250 245 135
NaeI GCC'GGC
Cuts at: 2371 2371
Size: 4190
NarI GG'CG_CC
Cuts at: 1621 1869 1621
Size: 248 3942
NgoMIV G'CCGG_C
Cuts at: 2369 2369
Size: 4190
NheI G'CTAG_C
Cuts at: 823 823
Size: 4190
NspI r_CATG'y
Cuts at: 1243 1743 2650 1243
Size: 500 907 2783
Fragments arranged by size:
2783 907 500
PciI A'CATG_T
Cuts at: 2646 2646
Size: 4190
PfoI T'CCnGG_A
Cuts at: 471 471
Size: 4190
PmeI GTTT'AAAC
Cuts at: 1189 1189
Size: 4190
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3329 3361 3329
Size: 32 4158
PstI C_TGCA'G
Cuts at: 1237 1922 1237
Size: 685 3505
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4190
PvuII CAG'CTG
Cuts at: 528 1615 1975 528
Size: 1087 360 2743
Fragments arranged by size:
2743 1087 360
RsrII CG'GwC_CG
Cuts at: 2385 2385
Size: 4190
SacI G_AGCT'C
Cuts at: 673 1204 673
Size: 531 3659
SalI G'TCGA_C
Cuts at: 1227 1227
Size: 4190
SapI GCTCTTCn'nnn_
Cuts at: 661 2213 2423 661
Size: 1552 210 2428
Fragments arranged by size:
2428 1552 210
SbfI CC_TGCA'GG
Cuts at: 1237 1237
Size: 4190
ScaI AGT'ACT
Cuts at: 1114 3707 1114
Size: 2593 1597
SexAI A'CCwGG_T
Cuts at: 960 960
Size: 4190
SfcI C'TryA_G
Cuts at: 33 1022 1233 1918 2911 3102 33
Size: 989 211 685 993 191 1121
Fragments arranged by size:
1121 993 989 685 211 191
SfoI GGC'GCC
Cuts at: 1622 1870 1622
Size: 248 3942
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4190
SmaI CCC'GGG
Cuts at: 1212 1212
Size: 4190
SmlI C'TyrA_G
Cuts at: 755 1345 2260 2752 3014 755
Size: 590 915 492 262 1931
Fragments arranged by size:
1931 915 590 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4190
SphI G_CATG'C
Cuts at: 1243 1743 1243
Size: 500 3690
StyI C'CwwG_G
Cuts at: 255 1324 1678 255
Size: 1069 354 2767
Fragments arranged by size:
2767 1069 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1773 1906 2365 2574 1773
Size: 133 459 209 3389
Fragments arranged by size:
3389 459 209 133
TatI w'GTAC_w
Cuts at: 983 1112 3705 983
Size: 129 2593 1468
Fragments arranged by size:
2593 1468 129
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 735 767 3707 3739 735
Size: 32 2940 32 1186
Fragments arranged by size:
2940 1186 32 32
Tth111I GACn'n_nGTC
Cuts at: 1987 1987
Size: 4190
XbaI T'CTAG_A
Cuts at: 1221 1221
Size: 4190
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1685 1685
Size: 4190
XmaI C'CCGG_G
Cuts at: 1210 1210
Size: 4190
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI
BanI BanII BbeI BbsI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NheI NspI PciI PfoI PmeI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI BbvCI BcgI BfrBI BmgBI BsaXI BseRI BsgI
BsiWI BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I
BtgI ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HpaI MfeI NcoI NdeI NotI NruI NsiI PacI
PasI PflMI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI