Restriction map for
pF1KB6510
(Circular) (Six-base) MAPSORT of: pF1KB6510.seq Check: 7171 from: 1 to: 4265
pF1KB6510 4265 bp
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AccI GT'mk_AC
Cuts at: 1303 1303
Size: 4265
Acc65I G'GTAC_C
Cuts at: 1281 1281
Size: 4265
AclI AA'CG_TT
Cuts at: 4105 4105
Size: 4265
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 166 667 794 2090 2522 3269 3537 166
Size: 501 127 1296 432 747 268 894
Fragments arranged by size:
1296 894 747 501 432 268 127
AflIII A'CryG_T
Cuts at: 1617 2721 3698 1617
Size: 1104 977 2184
Fragments arranged by size:
2184 1104 977
AgeI A'CCGG_T
Cuts at: 2653 2653
Size: 4265
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 404 998 404
Size: 594 3671
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 782 814 782
Size: 32 4233
AlwNI CAG_nnn'CTG
Cuts at: 658 1570 3137 658
Size: 912 1567 1786
Fragments arranged by size:
1786 1567 912
ApaLI G'TGCA_C
Cuts at: 329 3035 329
Size: 2706 1559
ApoI r'AATT_y
Cuts at: 217 246 1269 3618 3874 217
Size: 29 1023 2349 256 608
Fragments arranged by size:
2349 1023 608 256 29
AseI AT'TA_AT
Cuts at: 20 20
Size: 4265
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4265
AvaI C'yCGr_G
Cuts at: 1285 1285
Size: 4265
BamHI G'GATC_C
Cuts at: 1290 1290
Size: 4265
BanI G'GyrC_C
Cuts at: 1075 1281 1695 1943 1978 1075
Size: 206 414 248 35 3362
Fragments arranged by size:
3362 414 248 206 35
BanII G_rGCy'C
Cuts at: 215 1088 1279 2309 215
Size: 873 191 1030 2171
Fragments arranged by size:
2171 1030 873 191
BbeI G_GCGC'C
Cuts at: 1699 1947 1699
Size: 248 4017
BciVI GTATCCnnnnn_n'
Cuts at: 2163 2924 4265 2163
Size: 761 1341 2163
Fragments arranged by size:
2163 1341 761
BclI T'GATC_A
Cuts at: 1785 1785
Size: 4265
BglI GCCn_nnn'nGGC
Cuts at: 475 475
Size: 4265
BglII A'GATC_T
Cuts at: 279 1125 1780 3817 279
Size: 846 655 2037 727
Fragments arranged by size:
2037 846 727 655
BlpI GC'TnA_GC
Cuts at: 371 1377 371
Size: 1006 3259
Bme1580I G_kGCm'C
Cuts at: 333 878 1890 1983 3039 333
Size: 545 1012 93 1056 1559
Fragments arranged by size:
1559 1056 1012 545 93
BmrI ACTGGGnnnn_n'
Cuts at: 432 1477 1558 1893 4037 432
Size: 1045 81 335 2144 660
Fragments arranged by size:
2144 1045 660 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2632 2632
Size: 4265
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 397 859 397
Size: 462 3803
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1441 2320 2812 3110 1441
Size: 879 492 298 2596
Fragments arranged by size:
2596 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3969 3969
Size: 4265
BsaAI yAC'GTr
Cuts at: 1618 1618
Size: 4265
BsaBI GATnn'nnATC
Cuts at: 6 791 1779 3816 6
Size: 785 988 2037 455
Fragments arranged by size:
2037 988 785 455
BsaHI Gr'CG_yC
Cuts at: 1696 1944 2646 4142 1696
Size: 248 702 1496 1819
Fragments arranged by size:
1819 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1454 1975 2653 2927 3074 3485 1454
Size: 521 678 274 147 411 2234
Fragments arranged by size:
2234 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 880 910 1004 1034 880
Size: 30 94 30 4111
Fragments arranged by size:
4111 94 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 298 922 925 298
Size: 624 3 3638
Fragments arranged by size:
3638 624 3
BseYI C'CCAG_C
Cuts at: 1690 2448 3025 3521 1690
Size: 758 577 496 2434
Fragments arranged by size:
2434 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 289 671 994 289
Size: 382 323 3560
Fragments arranged by size:
3560 382 323
BsiEI CG_ry'CG
Cuts at: 104 1807 1853 3061 104
Size: 1703 46 1208 1308
Fragments arranged by size:
1703 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 333 1088 1279 2057 2247 3039 333
Size: 755 191 778 190 792 1559
Fragments arranged by size:
1559 792 778 755 191 190
Bsp1286I G_dGCh'C
Cuts at: 215 333 878 1088 1279 1890 1983 2057 2247
Size: 118 545 210 191 611 93 74 190
Cuts at: 2247 2309 3039 215
Size: 62 730 1441
Fragments arranged by size:
1441 730 611 545 210 191 190 118
93 74 62
BspEI T'CCGG_A
Cuts at: 3485 3485
Size: 4265
BspHI T'CATG_A
Cuts at: 903 3441 903
Size: 2538 1727
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1042 1315 1529 1831 2212 1042
Size: 273 214 302 381 3095
Fragments arranged by size:
3095 381 302 273 214
BsrBI CCG'CTC
Cuts at: 50 2557 2611 4095 4260 50
Size: 2507 54 1484 165 55
Fragments arranged by size:
2507 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2177 2177
Size: 4265
BsrFI r'CCGG_y
Cuts at: 2263 2444 2653 2263
Size: 181 209 3875
Fragments arranged by size:
3875 209 181
BssSI C'ACGA_G
Cuts at: 2536 2894 2536
Size: 358 3907
BstAPI GCAn_nnn'nTGC
Cuts at: 1642 1642
Size: 4265
BstBI TT'CG_AA
Cuts at: 2626 2626
Size: 4265
BstYI r'GATC_y
Cuts at: 1 279 752 1125 1290 1757 1780 2115 2361
Size: 278 473 373 165 467 23 335 246
Cuts at: 2361 3373 3459 3471 3817 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 473 467 449 373 346 335 278
246 165 86 23 12
BtgI C'CryG_G
Cuts at: 107 172 107
Size: 65 4200
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2215 2396 2559 2215
Size: 181 163 3921
Fragments arranged by size:
3921 181 163
BtsI GCAGTG_nn'
Cuts at: 1508 1508
Size: 4265
ClaI AT'CG_AT
Cuts at: 183 183
Size: 4265
DraI TTT'AAA
Cuts at: 1264 1264
Size: 4265
DrdI GACnn_nn'nnGTC
Cuts at: 1971 2829 1971
Size: 858 3407
EaeI y'GGCC_r
Cuts at: 286 444 1091 1214 1850 2024 2415 2442 3787
Size: 158 647 123 636 174 391 27 1345
Cuts at: 3787 286
Size: 764
Fragments arranged by size:
1345 764 647 636 391 174 158 123
27
EagI C'GGCC_G
Cuts at: 1850 1850
Size: 4265
EarI CTCTTCn'nnn_
Cuts at: 770 2288 2498 770
Size: 1518 210 2537
Fragments arranged by size:
2537 1518 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2473 2783 2929 4075 2473
Size: 310 146 1146 2663
Fragments arranged by size:
2663 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1086 1277 1086
Size: 191 4074
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 166 397 667 794 859 2090 2522 3269 3537
Size: 231 270 127 65 1231 432 747 268
Cuts at: 3537 166
Size: 894
Fragments arranged by size:
1231 894 747 432 270 268 231 127
65
EcoNI CCTnn'n_nnAGG
Cuts at: 904 904
Size: 4265
EcoO109I rG'GnC_Cy
Cuts at: 1404 1404
Size: 4265
EcoRI G'AATT_C
Cuts at: 1269 1269
Size: 4265
EcoRV GAT'ATC
Cuts at: 522 522
Size: 4265
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 994 2284 994
Size: 1290 2975
FspI TGC'GCA
Cuts at: 2046 2046
Size: 4265
HaeII r_GCGC'y
Cuts at: 1699 1947 2969 3948 1699
Size: 248 1022 979 2016
Fragments arranged by size:
2016 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 506 538 574 606 880 912 2107 2137 2139
Size: 32 36 32 274 32 1195 30 2
Cuts at: 2139 2169 506
Size: 30 2602
Fragments arranged by size:
2602 1195 274 36 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1304 1304
Size: 4265
KasI G'GCGC_C
Cuts at: 1695 1943 1695
Size: 248 4017
KpnI G_GTAC'C
Cuts at: 1285 1285
Size: 4265
MluI A'CGCG_T
Cuts at: 3698 3698
Size: 4265
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2936 3120 2936
Size: 184 4081
MscI TGG'CCA
Cuts at: 288 446 1093 1216 2026 288
Size: 158 647 123 810 2527
Fragments arranged by size:
2527 810 647 158 123
MslI CAynn'nnrTG
Cuts at: 365 764 2254 2381 365
Size: 399 1490 127 2249
Fragments arranged by size:
2249 1490 399 127
MspA1I CmG'CkG
Cuts at: 174 384 1376 1690 2050 3063 3308 174
Size: 210 992 314 360 1013 245 1131
Fragments arranged by size:
1131 1013 992 360 314 245 210
NaeI GCC'GGC
Cuts at: 2446 2446
Size: 4265
NarI GG'CG_CC
Cuts at: 1696 1944 1696
Size: 248 4017
NcoI C'CATG_G
Cuts at: 107 107
Size: 4265
NgoMIV G'CCGG_C
Cuts at: 2444 2444
Size: 4265
NruI TCG'CGA
Cuts at: 163 163
Size: 4265
NspI r_CATG'y
Cuts at: 1318 1818 2725 1318
Size: 500 907 2858
Fragments arranged by size:
2858 907 500
PciI A'CATG_T
Cuts at: 2721 2721
Size: 4265
PfoI T'CCnGG_A
Cuts at: 134 134
Size: 4265
PmeI GTTT'AAAC
Cuts at: 1264 1264
Size: 4265
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3404 3436 3404
Size: 32 4233
PstI C_TGCA'G
Cuts at: 434 456 484 623 1312 1997 434
Size: 22 28 139 689 685 2702
Fragments arranged by size:
2702 689 685 139 28 22
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4265
PvuII CAG'CTG
Cuts at: 384 1690 2050 384
Size: 1306 360 2599
Fragments arranged by size:
2599 1306 360
RsrII CG'GwC_CG
Cuts at: 2460 2460
Size: 4265
SacI G_AGCT'C
Cuts at: 1088 1279 1088
Size: 191 4074
SacII CC_GC'GG
Cuts at: 175 175
Size: 4265
SalI G'TCGA_C
Cuts at: 1302 1302
Size: 4265
SapI GCTCTTCn'nnn_
Cuts at: 2288 2498 2288
Size: 210 4055
SbfI CC_TGCA'GG
Cuts at: 1312 1312
Size: 4265
ScaI AGT'ACT
Cuts at: 571 3782 571
Size: 3211 1054
SfcI C'TryA_G
Cuts at: 33 430 452 480 619 745 1308 1993 2986
Size: 397 22 28 139 126 563 685 993
Cuts at: 2986 3177 33
Size: 191 1121
Fragments arranged by size:
1121 993 685 563 397 191 139 126
28 22
SfoI GGC'GCC
Cuts at: 1697 1945 1697
Size: 248 4017
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4265
SmaI CCC'GGG
Cuts at: 1287 1287
Size: 4265
SmlI C'TyrA_G
Cuts at: 1420 2335 2827 3089 1420
Size: 915 492 262 2596
Fragments arranged by size:
2596 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4265
SphI G_CATG'C
Cuts at: 1318 1818 1318
Size: 500 3765
StyI C'CwwG_G
Cuts at: 107 392 1399 1753 107
Size: 285 1007 354 2619
Fragments arranged by size:
2619 1007 354 285
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 547 1848 1981 2440 2649 547
Size: 1301 133 459 209 2163
Fragments arranged by size:
2163 1301 459 209 133
TatI w'GTAC_w
Cuts at: 569 1232 3780 569
Size: 663 2548 1054
Fragments arranged by size:
2548 1054 663
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3782 3814 3782
Size: 32 4233
Tth111I GACn'n_nGTC
Cuts at: 2062 2062
Size: 4265
XbaI T'CTAG_A
Cuts at: 1296 1296
Size: 4265
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1086 1760 1086
Size: 674 3591
XmaI C'CCGG_G
Cuts at: 1285 1285
Size: 4265
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI ClaI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NruI NspI PciI PfoI PmeI PpiI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI ApaI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BmgBI BmtI
Bpu10I BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I CspCI DraIII FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NsiI PacI PasI PflMI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI