Restriction map for
pF1KB6514
(Circular) (Six-base) MAPSORT of: pF1KB6514.seq Check: 7417 from: 1 to: 4226
pF1KB6514 4226 bp
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AccI GT'mk_AC
Cuts at: 983 1264 983
Size: 281 3945
Acc65I G'GTAC_C
Cuts at: 1160 1242 1160
Size: 82 4144
AclI AA'CG_TT
Cuts at: 4066 4066
Size: 4226
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2051 2483 3230 3498 2051
Size: 432 747 268 2779
Fragments arranged by size:
2779 747 432 268
AflIII A'CryG_T
Cuts at: 1578 2682 3659 1578
Size: 1104 977 2145
Fragments arranged by size:
2145 1104 977
AgeI A'CCGG_T
Cuts at: 2614 2614
Size: 4226
AleI CACnn'nnGTG
Cuts at: 631 631
Size: 4226
AlwNI CAG_nnn'CTG
Cuts at: 212 783 964 1531 3098 212
Size: 571 181 567 1567 1340
Fragments arranged by size:
1567 1340 571 567 181
ApaLI G'TGCA_C
Cuts at: 2996 2996
Size: 4226
ApoI r'AATT_y
Cuts at: 304 1230 3579 3835 304
Size: 926 2349 256 695
Fragments arranged by size:
2349 926 695 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4226
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4226
AvaI C'yCGr_G
Cuts at: 1246 1246
Size: 4226
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 576 609 576
Size: 33 4193
BamHI G'GATC_C
Cuts at: 1251 1251
Size: 4226
BanI G'GyrC_C
Cuts at: 1160 1242 1656 1904 1939 1160
Size: 82 414 248 35 3447
Fragments arranged by size:
3447 414 248 82 35
BanII G_rGCy'C
Cuts at: 499 1240 2270 499
Size: 741 1030 2455
Fragments arranged by size:
2455 1030 741
BbeI G_GCGC'C
Cuts at: 1660 1908 1660
Size: 248 3978
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1080 1114 1080
Size: 34 4192
BciVI GTATCCnnnnn_n'
Cuts at: 2124 2885 4226 2124
Size: 761 1341 2124
Fragments arranged by size:
2124 1341 761
BclI T'GATC_A
Cuts at: 618 1746 618
Size: 1128 3098
BglII A'GATC_T
Cuts at: 1741 3778 1741
Size: 2037 2189
BlpI GC'TnA_GC
Cuts at: 1338 1338
Size: 4226
Bme1580I G_kGCm'C
Cuts at: 1851 1944 3000 1851
Size: 93 1056 3077
Fragments arranged by size:
3077 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 974 1438 1519 1854 3998 974
Size: 464 81 335 2144 1202
Fragments arranged by size:
2144 1202 464 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2593 2593
Size: 4226
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 180 442 586 180
Size: 262 144 3820
Fragments arranged by size:
3820 262 144
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1402 2281 2773 3071 1402
Size: 879 492 298 2557
Fragments arranged by size:
2557 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 483 3930 483
Size: 3447 779
BsaAI yAC'GTr
Cuts at: 1579 1579
Size: 4226
BsaBI GATnn'nnATC
Cuts at: 6 1740 3777 6
Size: 1734 2037 455
Fragments arranged by size:
2037 1734 455
BsaHI Gr'CG_yC
Cuts at: 1657 1905 2607 4103 1657
Size: 248 702 1496 1780
Fragments arranged by size:
1780 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1415 1936 2614 2888 3035 3446 1415
Size: 521 678 274 147 411 2195
Fragments arranged by size:
2195 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 315 345 440 470 1163 1193 315
Size: 30 95 30 693 30 3348
Fragments arranged by size:
3348 693 95 30 30 30
BseYI C'CCAG_C
Cuts at: 715 760 791 1651 2409 2986 3482 715
Size: 45 31 860 758 577 496 1459
Fragments arranged by size:
1459 860 758 577 496 45 31
BsiEI CG_ry'CG
Cuts at: 104 591 1768 1814 3022 104
Size: 487 1177 46 1208 1308
Fragments arranged by size:
1308 1208 1177 487 46
BsiHKAI G_wGCw'C
Cuts at: 499 1240 2018 2208 3000 499
Size: 741 778 190 792 1725
Fragments arranged by size:
1725 792 778 741 190
Bsp1286I G_dGCh'C
Cuts at: 499 1240 1851 1944 2018 2208 2270 3000 499
Size: 741 611 93 74 190 62 730 1725
Fragments arranged by size:
1725 741 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3446 3446
Size: 4226
BspHI T'CATG_A
Cuts at: 3402 3402
Size: 4226
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1276 1490 1792 2173 1276
Size: 214 302 381 3329
Fragments arranged by size:
3329 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2518 2572 4056 4221 50
Size: 2468 54 1484 165 55
Fragments arranged by size:
2468 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 192 2138 192
Size: 1946 2280
BsrFI r'CCGG_y
Cuts at: 812 2224 2405 2614 812
Size: 1412 181 209 2424
Fragments arranged by size:
2424 1412 209 181
BssSI C'ACGA_G
Cuts at: 2497 2855 2497
Size: 358 3868
BstAPI GCAn_nnn'nTGC
Cuts at: 1603 1603
Size: 4226
BstBI TT'CG_AA
Cuts at: 2587 2587
Size: 4226
BstXI CCAn_nnnn'nTGG
Cuts at: 645 645
Size: 4226
BstYI r'GATC_y
Cuts at: 1 1251 1718 1741 2076 2322 3334 3420 3432
Size: 1250 467 23 335 246 1012 86 12
Cuts at: 3432 3778 1
Size: 346 449
Fragments arranged by size:
1250 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 152 152
Size: 4226
BtgI C'CryG_G
Cuts at: 107 143 1136 107
Size: 36 993 3197
Fragments arranged by size:
3197 993 36
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1128 2176 2357 2520 1128
Size: 1048 181 163 2834
Fragments arranged by size:
2834 1048 181 163
BtsI GCAGTG_nn'
Cuts at: 1052 1469 1052
Size: 417 3809
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 914 949 914
Size: 35 4191
DraI TTT'AAA
Cuts at: 443 1197 1225 443
Size: 754 28 3444
Fragments arranged by size:
3444 754 28
DrdI GACnn_nn'nnGTC
Cuts at: 1932 2790 1932
Size: 858 3368
EaeI y'GGCC_r
Cuts at: 588 705 1133 1139 1811 1985 2376 2403 3748
Size: 117 428 6 672 174 391 27 1345
Cuts at: 3748 588
Size: 1066
Fragments arranged by size:
1345 1066 672 428 391 174 117 27
6
EagI C'GGCC_G
Cuts at: 588 1811 588
Size: 1223 3003
EarI CTCTTCn'nnn_
Cuts at: 322 2249 2459 322
Size: 1927 210 2089
Fragments arranged by size:
2089 1927 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2434 2744 2890 4036 2434
Size: 310 146 1146 2624
Fragments arranged by size:
2624 1146 310 146
EcoICRI GAG'CTC
Cuts at: 497 1238 497
Size: 741 3485
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 180 442 586 2051 2483 3230 3498 180
Size: 262 144 1465 432 747 268 908
Fragments arranged by size:
1465 908 747 432 268 262 144
EcoNI CCTnn'n_nnAGG
Cuts at: 507 507
Size: 4226
EcoO109I rG'GnC_Cy
Cuts at: 780 1365 780
Size: 585 3641
EcoRI G'AATT_C
Cuts at: 304 1230 304
Size: 926 3300
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2245 2245
Size: 4226
FspI TGC'GCA
Cuts at: 2007 2007
Size: 4226
HaeII r_GCGC'y
Cuts at: 819 1660 1908 2930 3909 819
Size: 841 248 1022 979 1136
Fragments arranged by size:
1136 1022 979 841 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 440 472 2068 2098 2100 2130 440
Size: 32 1596 30 2 30 2536
Fragments arranged by size:
2536 1596 32 30 30 2
HincII GTy'rAC
Cuts at: 273 399 1265 273
Size: 126 866 3234
Fragments arranged by size:
3234 866 126
HpaI GTT'AAC
Cuts at: 399 399
Size: 4226
KasI G'GCGC_C
Cuts at: 1656 1904 1656
Size: 248 3978
KpnI G_GTAC'C
Cuts at: 1164 1246 1164
Size: 82 4144
MluI A'CGCG_T
Cuts at: 3659 3659
Size: 4226
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 193 2897 3081 193
Size: 2704 184 1338
Fragments arranged by size:
2704 1338 184
MscI TGG'CCA
Cuts at: 707 1141 1987 707
Size: 434 846 2946
Fragments arranged by size:
2946 846 434
MslI CAynn'nnrTG
Cuts at: 631 2215 2342 631
Size: 1584 127 2515
Fragments arranged by size:
2515 1584 127
MspA1I CmG'CkG
Cuts at: 719 1337 1651 2011 3024 3269 719
Size: 618 314 360 1013 245 1676
Fragments arranged by size:
1676 1013 618 360 314 245
NaeI GCC'GGC
Cuts at: 2407 2407
Size: 4226
NarI GG'CG_CC
Cuts at: 1657 1905 1657
Size: 248 3978
NcoI C'CATG_G
Cuts at: 107 143 1136 107
Size: 36 993 3197
Fragments arranged by size:
3197 993 36
NdeI CA'TA_TG
Cuts at: 483 483
Size: 4226
NgoMIV G'CCGG_C
Cuts at: 2405 2405
Size: 4226
NspI r_CATG'y
Cuts at: 1279 1779 2686 1279
Size: 500 907 2819
Fragments arranged by size:
2819 907 500
PciI A'CATG_T
Cuts at: 2682 2682
Size: 4226
PflMI CCAn_nnn'nTGG
Cuts at: 113 113
Size: 4226
PmeI GTTT'AAAC
Cuts at: 1225 1225
Size: 4226
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3365 3397 3365
Size: 32 4194
PstI C_TGCA'G
Cuts at: 1273 1958 1273
Size: 685 3541
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4226
PvuII CAG'CTG
Cuts at: 1651 2011 1651
Size: 360 3866
RsrII CG'GwC_CG
Cuts at: 2421 2421
Size: 4226
SacI G_AGCT'C
Cuts at: 499 1240 499
Size: 741 3485
SalI G'TCGA_C
Cuts at: 1263 1263
Size: 4226
SapI GCTCTTCn'nnn_
Cuts at: 2249 2459 2249
Size: 210 4016
SbfI CC_TGCA'GG
Cuts at: 1273 1273
Size: 4226
ScaI AGT'ACT
Cuts at: 3743 3743
Size: 4226
SfcI C'TryA_G
Cuts at: 33 1269 1954 2947 3138 33
Size: 1236 685 993 191 1121
Fragments arranged by size:
1236 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1658 1906 1658
Size: 248 3978
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4226
SmaI CCC'GGG
Cuts at: 1248 1248
Size: 4226
SmlI C'TyrA_G
Cuts at: 1381 2296 2788 3050 1381
Size: 915 492 262 2557
Fragments arranged by size:
2557 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4226
SphI G_CATG'C
Cuts at: 1279 1779 1279
Size: 500 3726
StyI C'CwwG_G
Cuts at: 107 143 1136 1360 1714 107
Size: 36 993 224 354 2619
Fragments arranged by size:
2619 993 354 224 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 956 1809 1942 2401 2610 956
Size: 853 133 459 209 2572
Fragments arranged by size:
2572 853 459 209 133
TatI w'GTAC_w
Cuts at: 3741 3741
Size: 4226
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 315 347 1163 1195 3743 3775 315
Size: 32 816 32 2548 32 766
Fragments arranged by size:
2548 816 766 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2023 2023
Size: 4226
XbaI T'CTAG_A
Cuts at: 1257 1257
Size: 4226
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1721 1721
Size: 4226
XmaI C'CCGG_G
Cuts at: 1246 1246
Size: 4226
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BcgI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HpaI KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NspI PciI PflMI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BbsI BbvCI BfrBI BglI BmgBI BmtI
Bpu10I BseRI BsgI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstZ17I ClaI DraIII EcoRV FseI FspAI HindIII
MfeI NheI NotI NruI NsiI PacI PasI PfoI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI