Restriction map for
pF1KB6517
(Circular) (Six-base) MAPSORT of: pF1KB6517.seq Check: 9096 from: 1 to: 4199
pF1KB6517 4199 bp
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AccI GT'mk_AC
Cuts at: 168 1237 168
Size: 1069 3130
Acc65I G'GTAC_C
Cuts at: 1215 1215
Size: 4199
AclI AA'CG_TT
Cuts at: 4039 4039
Size: 4199
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1152 2024 2456 3203 3471 1152
Size: 872 432 747 268 1880
Fragments arranged by size:
1880 872 747 432 268
AflIII A'CryG_T
Cuts at: 1551 2655 3632 1551
Size: 1104 977 2118
Fragments arranged by size:
2118 1104 977
AgeI A'CCGG_T
Cuts at: 2587 2587
Size: 4199
AhdI GACnn_n'nnGTC
Cuts at: 914 914
Size: 4199
AlwNI CAG_nnn'CTG
Cuts at: 1504 3071 1504
Size: 1567 2632
ApaLI G'TGCA_C
Cuts at: 122 2969 122
Size: 2847 1352
ApoI r'AATT_y
Cuts at: 855 1115 1158 1203 3552 3808 855
Size: 260 43 45 2349 256 1246
Fragments arranged by size:
2349 1246 260 256 45 43
AseI AT'TA_AT
Cuts at: 20 20
Size: 4199
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4199
AvaI C'yCGr_G
Cuts at: 1219 1219
Size: 4199
BamHI G'GATC_C
Cuts at: 694 1224 694
Size: 530 3669
BanI G'GyrC_C
Cuts at: 1215 1629 1877 1912 1215
Size: 414 248 35 3502
Fragments arranged by size:
3502 414 248 35
BanII G_rGCy'C
Cuts at: 1213 2243 1213
Size: 1030 3169
BbeI G_GCGC'C
Cuts at: 1633 1881 1633
Size: 248 3951
BbsI GAAGACnn'nnnn_
Cuts at: 139 310 715 139
Size: 171 405 3623
Fragments arranged by size:
3623 405 171
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4165
BciVI GTATCCnnnnn_n'
Cuts at: 2097 2858 4199 2097
Size: 761 1341 2097
Fragments arranged by size:
2097 1341 761
BclI T'GATC_A
Cuts at: 1719 1719
Size: 4199
BglII A'GATC_T
Cuts at: 700 1077 1714 3751 700
Size: 377 637 2037 1148
Fragments arranged by size:
2037 1148 637 377
BlpI GC'TnA_GC
Cuts at: 1311 1311
Size: 4199
Bme1580I G_kGCm'C
Cuts at: 126 440 1824 1917 2973 126
Size: 314 1384 93 1056 1352
Fragments arranged by size:
1384 1352 1056 314 93
BmrI ACTGGGnnnn_n'
Cuts at: 1411 1492 1827 3971 1411
Size: 81 335 2144 1639
Fragments arranged by size:
2144 1639 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2566 2566
Size: 4199
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 365 920 365
Size: 555 3644
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 894 1117 1375 2254 2746 3044 894
Size: 223 258 879 492 298 2049
Fragments arranged by size:
2049 879 492 298 258 223
BsaI GGTCTCn'nnnn_
Cuts at: 3903 3903
Size: 4199
BsaAI yAC'GTr
Cuts at: 1552 1552
Size: 4199
BsaBI GATnn'nnATC
Cuts at: 6 699 1713 3750 6
Size: 693 1014 2037 455
Fragments arranged by size:
2037 1014 693 455
BsaHI Gr'CG_yC
Cuts at: 1630 1878 2580 4076 1630
Size: 248 702 1496 1753
Fragments arranged by size:
1753 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1388 1909 2587 2861 3008 3419 1388
Size: 521 678 274 147 411 2168
Fragments arranged by size:
2168 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 265 295 265
Size: 30 4169
BseYI C'CCAG_C
Cuts at: 1624 2382 2959 3455 1624
Size: 758 577 496 2368
Fragments arranged by size:
2368 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 390 528 588 390
Size: 138 60 4001
Fragments arranged by size:
4001 138 60
BsiEI CG_ry'CG
Cuts at: 104 1741 1787 2995 104
Size: 1637 46 1208 1308
Fragments arranged by size:
1637 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 126 1213 1991 2181 2973 126
Size: 1087 778 190 792 1352
Fragments arranged by size:
1352 1087 792 778 190
BsmI GAATG_Cn'
Cuts at: 661 661
Size: 4199
Bsp1286I G_dGCh'C
Cuts at: 126 440 1213 1824 1917 1991 2181 2243 2973
Size: 314 773 611 93 74 190 62 730
Cuts at: 2973 126
Size: 1352
Fragments arranged by size:
1352 773 730 611 314 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3419 3419
Size: 4199
BspHI T'CATG_A
Cuts at: 348 816 905 3375 348
Size: 468 89 2470 1172
Fragments arranged by size:
2470 1172 468 89
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1249 1463 1765 2146 1249
Size: 214 302 381 3302
Fragments arranged by size:
3302 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2491 2545 4029 4194 50
Size: 2441 54 1484 165 55
Fragments arranged by size:
2441 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2111 2111
Size: 4199
BsrFI r'CCGG_y
Cuts at: 2197 2378 2587 2197
Size: 181 209 3809
Fragments arranged by size:
3809 209 181
BssSI C'ACGA_G
Cuts at: 2470 2828 2470
Size: 358 3841
BstAPI GCAn_nnn'nTGC
Cuts at: 1576 1576
Size: 4199
BstBI TT'CG_AA
Cuts at: 936 2560 936
Size: 1624 2575
BstXI CCAn_nnnn'nTGG
Cuts at: 291 291
Size: 4199
BstYI r'GATC_y
Cuts at: 1 694 700 1077 1224 1691 1714 2049 2295
Size: 693 6 377 147 467 23 335 246
Cuts at: 2295 3307 3393 3405 3751 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 693 467 449 377 346 335 246
147 86 23 12 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2149 2330 2493 2149
Size: 181 163 3855
Fragments arranged by size:
3855 181 163
BtsI GCAGTG_nn'
Cuts at: 1442 1442
Size: 4199
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 197 232 197
Size: 35 4164
DraI TTT'AAA
Cuts at: 1198 1198
Size: 4199
DraIII CAC_nnn'GTG
Cuts at: 680 680
Size: 4199
DrdI GACnn_nn'nnGTC
Cuts at: 409 1905 2763 409
Size: 1496 858 1845
Fragments arranged by size:
1845 1496 858
EaeI y'GGCC_r
Cuts at: 354 1784 1958 2349 2376 3721 354
Size: 1430 174 391 27 1345 832
Fragments arranged by size:
1430 1345 832 391 174 27
EagI C'GGCC_G
Cuts at: 1784 1784
Size: 4199
EarI CTCTTCn'nnn_
Cuts at: 754 2222 2432 754
Size: 1468 210 2521
Fragments arranged by size:
2521 1468 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2407 2717 2863 4009 2407
Size: 310 146 1146 2597
Fragments arranged by size:
2597 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1211 1211
Size: 4199
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 365 920 1152 2024 2456 3203 3471 365
Size: 555 232 872 432 747 268 1093
Fragments arranged by size:
1093 872 747 555 432 268 232
EcoO109I rG'GnC_Cy
Cuts at: 1338 1338
Size: 4199
EcoRI G'AATT_C
Cuts at: 1203 1203
Size: 4199
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1111 2218 1111
Size: 1107 3092
FspI TGC'GCA
Cuts at: 1980 1980
Size: 4199
HaeII r_GCGC'y
Cuts at: 1633 1881 2903 3882 1633
Size: 248 1022 979 1950
Fragments arranged by size:
1950 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 265 297 679 711 900 932 1062 1094 2041
Size: 32 382 32 189 32 130 32 947
Cuts at: 2041 2071 2073 2103 265
Size: 30 2 30 2361
Fragments arranged by size:
2361 947 382 189 130 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 870 1238 870
Size: 368 3831
HindIII A'AGCT_T
Cuts at: 1185 1185
Size: 4199
KasI G'GCGC_C
Cuts at: 1629 1877 1629
Size: 248 3951
KpnI G_GTAC'C
Cuts at: 1219 1219
Size: 4199
MluI A'CGCG_T
Cuts at: 3632 3632
Size: 4199
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 850 2870 3054 850
Size: 2020 184 1995
Fragments arranged by size:
2020 1995 184
MscI TGG'CCA
Cuts at: 356 1960 356
Size: 1604 2595
MslI CAynn'nnrTG
Cuts at: 350 2188 2315 350
Size: 1838 127 2234
Fragments arranged by size:
2234 1838 127
MspA1I CmG'CkG
Cuts at: 1310 1624 1984 2997 3242 1310
Size: 314 360 1013 245 2267
Fragments arranged by size:
2267 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2380 2380
Size: 4199
NarI GG'CG_CC
Cuts at: 1630 1878 1630
Size: 248 3951
NgoMIV G'CCGG_C
Cuts at: 2378 2378
Size: 4199
NspI r_CATG'y
Cuts at: 785 1252 1752 2659 785
Size: 467 500 907 2325
Fragments arranged by size:
2325 907 500 467
PasI CC'CwG_GG
Cuts at: 141 141
Size: 4199
PciI A'CATG_T
Cuts at: 2655 2655
Size: 4199
PflMI CCAn_nnn'nTGG
Cuts at: 1188 1188
Size: 4199
PmeI GTTT'AAAC
Cuts at: 1198 1198
Size: 4199
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3338 3370 3338
Size: 32 4167
PstI C_TGCA'G
Cuts at: 1246 1931 1246
Size: 685 3514
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4199
PvuII CAG'CTG
Cuts at: 1624 1984 1624
Size: 360 3839
RsrII CG'GwC_CG
Cuts at: 2394 2394
Size: 4199
SacI G_AGCT'C
Cuts at: 1213 1213
Size: 4199
SalI G'TCGA_C
Cuts at: 1236 1236
Size: 4199
SapI GCTCTTCn'nnn_
Cuts at: 2222 2432 2222
Size: 210 3989
SbfI CC_TGCA'GG
Cuts at: 1246 1246
Size: 4199
ScaI AGT'ACT
Cuts at: 3716 3716
Size: 4199
SfcI C'TryA_G
Cuts at: 33 314 1242 1927 2920 3111 33
Size: 281 928 685 993 191 1121
Fragments arranged by size:
1121 993 928 685 281 191
SfoI GGC'GCC
Cuts at: 1631 1879 1631
Size: 248 3951
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4199
SmaI CCC'GGG
Cuts at: 1221 1221
Size: 4199
SmlI C'TyrA_G
Cuts at: 873 1132 1354 2269 2761 3023 873
Size: 259 222 915 492 262 2049
Fragments arranged by size:
2049 915 492 262 259 222
SpeI A'CTAG_T
Cuts at: 67 1144 67
Size: 1077 3122
SphI G_CATG'C
Cuts at: 1252 1752 1252
Size: 500 3699
StuI AGG'CCT
Cuts at: 341 341
Size: 4199
StyI C'CwwG_G
Cuts at: 284 807 1333 1687 284
Size: 523 526 354 2796
Fragments arranged by size:
2796 526 523 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 744 1782 1915 2374 2583 744
Size: 1038 133 459 209 2360
Fragments arranged by size:
2360 1038 459 209 133
TatI w'GTAC_w
Cuts at: 458 771 1099 3714 458
Size: 313 328 2615 943
Fragments arranged by size:
2615 943 328 313
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3716 3748 3716
Size: 32 4167
Tth111I GACn'n_nGTC
Cuts at: 985 1996 985
Size: 1011 3188
XbaI T'CTAG_A
Cuts at: 1230 1230
Size: 4199
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 705 749 1694 705
Size: 44 945 3210
Fragments arranged by size:
3210 945 44
XmaI C'CCGG_G
Cuts at: 1219 1219
Size: 4199
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BtgZI BtsI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PasI PciI PflMI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BfrBI BglI BmgBI BmtI
Bpu10I BseRI BsiWI BsmBI BsrGI BssHII BstEII BstZ17I
Bsu36I BtgI ClaI EcoNI EcoRV FseI FspAI HpaI
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI XmnI ZraI