Restriction map for
pF1KB6518
(Circular) (Six-base) MAPSORT of: pF1KB6518.seq Check: 2648 from: 1 to: 4271
pF1KB6518 4271 bp
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 402 962 1065 402
Size: 560 103 3608
Fragments arranged by size:
3608 560 103
AccI GT'mk_AC
Cuts at: 557 1309 557
Size: 752 3519
Acc65I G'GTAC_C
Cuts at: 1287 1287
Size: 4271
AclI AA'CG_TT
Cuts at: 4111 4111
Size: 4271
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 222 304 925 2096 2528 3275 3543 222
Size: 82 621 1171 432 747 268 950
Fragments arranged by size:
1171 950 747 621 432 268 82
AflIII A'CryG_T
Cuts at: 1623 2727 3704 1623
Size: 1104 977 2190
Fragments arranged by size:
2190 1104 977
AgeI A'CCGG_T
Cuts at: 2659 2659
Size: 4271
AleI CACnn'nnGTG
Cuts at: 211 211
Size: 4271
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 991 991
Size: 4271
AlwNI CAG_nnn'CTG
Cuts at: 245 483 612 1576 3143 245
Size: 238 129 964 1567 1373
Fragments arranged by size:
1567 1373 964 238 129
ApaI G_GGCC'C
Cuts at: 201 201
Size: 4271
ApaLI G'TGCA_C
Cuts at: 3041 3041
Size: 4271
ApoI r'AATT_y
Cuts at: 1275 3624 3880 1275
Size: 2349 256 1666
Fragments arranged by size:
2349 1666 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4271
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4271
AvaI C'yCGr_G
Cuts at: 129 1291 129
Size: 1162 3109
BamHI G'GATC_C
Cuts at: 1296 1296
Size: 4271
BanI G'GyrC_C
Cuts at: 1047 1287 1701 1949 1984 1047
Size: 240 414 248 35 3334
Fragments arranged by size:
3334 414 248 240 35
BanII G_rGCy'C
Cuts at: 122 201 754 1285 2315 122
Size: 79 553 531 1030 2078
Fragments arranged by size:
2078 1030 553 531 79
BbeI G_GCGC'C
Cuts at: 1705 1953 1705
Size: 248 4023
BciVI GTATCCnnnnn_n'
Cuts at: 2169 2930 4271 2169
Size: 761 1341 2169
Fragments arranged by size:
2169 1341 761
BclI T'GATC_A
Cuts at: 1791 1791
Size: 4271
BglI GCCn_nnn'nGGC
Cuts at: 1144 1159 1144
Size: 15 4256
BglII A'GATC_T
Cuts at: 1786 3823 1786
Size: 2037 2234
BlpI GC'TnA_GC
Cuts at: 1383 1383
Size: 4271
Bme1580I G_kGCm'C
Cuts at: 191 201 1052 1896 1989 3045 191
Size: 10 851 844 93 1056 1417
Fragments arranged by size:
1417 1056 851 844 93 10
BmrI ACTGGGnnnn_n'
Cuts at: 178 1185 1209 1483 1564 1899 4043 178
Size: 1007 24 274 81 335 2144 406
Fragments arranged by size:
2144 1007 406 335 274 81 24
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2638 2638
Size: 4271
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 147 403 910 147
Size: 256 507 3508
Fragments arranged by size:
3508 507 256
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1447 2326 2818 3116 1447
Size: 879 492 298 2602
Fragments arranged by size:
2602 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 225 583 625 3975 225
Size: 358 42 3350 521
Fragments arranged by size:
3350 521 358 42
BsaAI yAC'GTr
Cuts at: 657 1624 657
Size: 967 3304
BsaBI GATnn'nnATC
Cuts at: 6 1785 3822 6
Size: 1779 2037 455
Fragments arranged by size:
2037 1779 455
BsaHI Gr'CG_yC
Cuts at: 722 1702 1950 2652 4148 722
Size: 980 248 702 1496 845
Fragments arranged by size:
1496 980 845 702 248
BsaWI w'CCGG_w
Cuts at: 260 1460 1981 2659 2933 3080 3491 260
Size: 1200 521 678 274 147 411 1040
Fragments arranged by size:
1200 1040 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 220 391 525 826 220
Size: 171 134 301 3665
Fragments arranged by size:
3665 301 171 134
BseYI C'CCAG_C
Cuts at: 336 663 1107 1696 2454 3031 3527 336
Size: 327 444 589 758 577 496 1080
Fragments arranged by size:
1080 758 589 577 496 444 327
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 382 796 1093 382
Size: 414 297 3560
Fragments arranged by size:
3560 414 297
BsiEI CG_ry'CG
Cuts at: 104 705 866 1151 1813 1859 3067 104
Size: 601 161 285 662 46 1208 1308
Fragments arranged by size:
1308 1208 662 601 285 161 46
BsiHKAI G_wGCw'C
Cuts at: 754 1285 2063 2253 3045 754
Size: 531 778 190 792 1980
Fragments arranged by size:
1980 792 778 531 190
BsmI GAATG_Cn'
Cuts at: 491 491
Size: 4271
Bsp1286I G_dGCh'C
Cuts at: 122 191 201 754 1052 1285 1896 1989 2063
Size: 69 10 553 298 233 611 93 74
Cuts at: 2063 2253 2315 3045 122
Size: 190 62 730 1348
Fragments arranged by size:
1348 730 611 553 298 233 190 93
74 69 62 10
BspEI T'CCGG_A
Cuts at: 3491 3491
Size: 4271
BspHI T'CATG_A
Cuts at: 3447 3447
Size: 4271
BspMI ACCTGCnnnn'nnnn_
Cuts at: 402 569 962 1065 1321 1535 1837 2218 402
Size: 167 393 103 256 214 302 381 2455
Fragments arranged by size:
2455 393 381 302 256 214 167 103
BsrBI CCG'CTC
Cuts at: 50 2563 2617 4101 4266 50
Size: 2513 54 1484 165 55
Fragments arranged by size:
2513 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 509 723 1208 2183 509
Size: 214 485 975 2597
Fragments arranged by size:
2597 975 485 214
BsrFI r'CCGG_y
Cuts at: 1153 2269 2450 2659 1153
Size: 1116 181 209 2765
Fragments arranged by size:
2765 1116 209 181
BssSI C'ACGA_G
Cuts at: 2542 2900 2542
Size: 358 3913
BstAPI GCAn_nnn'nTGC
Cuts at: 1227 1648 1227
Size: 421 3850
BstBI TT'CG_AA
Cuts at: 2632 2632
Size: 4271
BstXI CCAn_nnnn'nTGG
Cuts at: 216 216
Size: 4271
BstYI r'GATC_y
Cuts at: 1 522 933 1296 1763 1786 2121 2367 3379
Size: 521 411 363 467 23 335 246 1012
Cuts at: 3379 3465 3477 3823 1
Size: 86 12 346 449
Fragments arranged by size:
1012 521 467 449 411 363 346 335
246 86 23 12
BtgI C'CryG_G
Cuts at: 177 755 805 860 1139 1198 177
Size: 578 50 55 279 59 3250
Fragments arranged by size:
3250 578 279 59 55 50
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 125 2221 2402 2565 125
Size: 2096 181 163 1831
Fragments arranged by size:
2096 1831 181 163
BtsI GCAGTG_nn'
Cuts at: 1514 1514
Size: 4271
DraI TTT'AAA
Cuts at: 1270 1270
Size: 4271
DrdI GACnn_nn'nnGTC
Cuts at: 1977 2835 1977
Size: 858 3413
EaeI y'GGCC_r
Cuts at: 203 474 769 863 1136 1148 1190 1856 2030
Size: 271 295 94 273 12 42 666 174
Cuts at: 2030 2421 2448 3793 203
Size: 391 27 1345 681
Fragments arranged by size:
1345 681 666 391 295 273 271 174
94 42 27 12
EagI C'GGCC_G
Cuts at: 863 1148 1856 863
Size: 285 708 3278
Fragments arranged by size:
3278 708 285
EarI CTCTTCn'nnn_
Cuts at: 259 286 2294 2504 259
Size: 27 2008 210 2026
Fragments arranged by size:
2026 2008 210 27
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 294 1195 2479 2789 2935 4081 294
Size: 901 1284 310 146 1146 484
Fragments arranged by size:
1284 1146 901 484 310 146
EcoICRI GAG'CTC
Cuts at: 752 1283 752
Size: 531 3740
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 147 222 304 403 910 925 2096 2528 3275
Size: 75 82 99 507 15 1171 432 747
Cuts at: 3275 3543 147
Size: 268 875
Fragments arranged by size:
1171 875 747 507 432 268 99 82
75 15
EcoNI CCTnn'n_nnAGG
Cuts at: 958 958
Size: 4271
EcoO109I rG'GnC_Cy
Cuts at: 133 198 648 741 1410 133
Size: 65 450 93 669 2994
Fragments arranged by size:
2994 669 450 93 65
EcoRI G'AATT_C
Cuts at: 1275 1275
Size: 4271
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2290 2290
Size: 4271
FspI TGC'GCA
Cuts at: 2052 2052
Size: 4271
HaeII r_GCGC'y
Cuts at: 1705 1953 2975 3954 1705
Size: 248 1022 979 2022
Fragments arranged by size:
2022 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 694 726 2113 2143 2145 2175 694
Size: 32 1387 30 2 30 2790
Fragments arranged by size:
2790 1387 32 30 30 2
HincII GTy'rAC
Cuts at: 1310 1310
Size: 4271
HindIII A'AGCT_T
Cuts at: 823 1031 823
Size: 208 4063
KasI G'GCGC_C
Cuts at: 1701 1949 1701
Size: 248 4023
KpnI G_GTAC'C
Cuts at: 1291 1291
Size: 4271
MluI A'CGCG_T
Cuts at: 3704 3704
Size: 4271
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 253 2942 3126 253
Size: 2689 184 1398
Fragments arranged by size:
2689 1398 184
MscI TGG'CCA
Cuts at: 205 476 1192 2032 205
Size: 271 716 840 2444
Fragments arranged by size:
2444 840 716 271
MslI CAynn'nnrTG
Cuts at: 211 688 2260 2387 211
Size: 477 1572 127 2095
Fragments arranged by size:
2095 1572 477 127
MspA1I CmG'CkG
Cuts at: 242 480 807 1200 1382 1696 2056 3069 3314
Size: 238 327 393 182 314 360 1013 245
Cuts at: 3314 242
Size: 1199
Fragments arranged by size:
1199 1013 393 360 327 314 245 238
182
NaeI GCC'GGC
Cuts at: 1155 2452 1155
Size: 1297 2974
NarI GG'CG_CC
Cuts at: 1702 1950 1702
Size: 248 4023
NgoMIV G'CCGG_C
Cuts at: 1153 2450 1153
Size: 1297 2974
NotI GC'GGCC_GC
Cuts at: 1148 1148
Size: 4271
NspI r_CATG'y
Cuts at: 1188 1324 1824 2731 1188
Size: 136 500 907 2728
Fragments arranged by size:
2728 907 500 136
PasI CC'CwG_GG
Cuts at: 194 194
Size: 4271
PciI A'CATG_T
Cuts at: 2727 2727
Size: 4271
PflMI CCAn_nnn'nTGG
Cuts at: 483 483
Size: 4271
PfoI T'CCnGG_A
Cuts at: 113 887 113
Size: 774 3497
PmeI GTTT'AAAC
Cuts at: 1270 1270
Size: 4271
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3410 3442 3410
Size: 32 4239
PpuMI rG'GwC_Cy
Cuts at: 648 741 648
Size: 93 4178
PshAI GACnn'nnGTC
Cuts at: 164 164
Size: 4271
PspOMI G'GGCC_C
Cuts at: 197 197
Size: 4271
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1006 1038 1006
Size: 32 4239
PstI C_TGCA'G
Cuts at: 446 797 962 1318 2003 446
Size: 351 165 356 685 2714
Fragments arranged by size:
2714 685 356 351 165
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4271
PvuII CAG'CTG
Cuts at: 242 480 1696 2056 242
Size: 238 1216 360 2457
Fragments arranged by size:
2457 1216 360 238
RsrII CG'GwC_CG
Cuts at: 1239 2466 1239
Size: 1227 3044
SacI G_AGCT'C
Cuts at: 754 1285 754
Size: 531 3740
SacII CC_GC'GG
Cuts at: 808 1201 808
Size: 393 3878
SalI G'TCGA_C
Cuts at: 1308 1308
Size: 4271
SanDI GG'GwC_CC
Cuts at: 741 741
Size: 4271
SapI GCTCTTCn'nnn_
Cuts at: 259 286 2294 2504 259
Size: 27 2008 210 2026
Fragments arranged by size:
2026 2008 210 27
SbfI CC_TGCA'GG
Cuts at: 1318 1318
Size: 4271
ScaI AGT'ACT
Cuts at: 448 3788 448
Size: 3340 931
SfcI C'TryA_G
Cuts at: 33 442 793 958 1314 1999 2992 3183 33
Size: 409 351 165 356 685 993 191 1121
Fragments arranged by size:
1121 993 685 409 356 351 191 165
SfoI GGC'GCC
Cuts at: 1703 1951 1703
Size: 248 4023
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4271
SgrAI Cr'CCGG_yG
Cuts at: 1153 1153
Size: 4271
SmaI CCC'GGG
Cuts at: 131 1293 131
Size: 1162 3109
SmlI C'TyrA_G
Cuts at: 1426 2341 2833 3095 1426
Size: 915 492 262 2602
Fragments arranged by size:
2602 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4271
SphI G_CATG'C
Cuts at: 1324 1824 1324
Size: 500 3771
StuI AGG'CCT
Cuts at: 227 781 227
Size: 554 3717
StyI C'CwwG_G
Cuts at: 518 593 1405 1759 518
Size: 75 812 354 3030
Fragments arranged by size:
3030 812 354 75
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1256 1854 1987 2446 2655 1256
Size: 598 133 459 209 2872
Fragments arranged by size:
2872 598 459 209 133
TatI w'GTAC_w
Cuts at: 446 3786 446
Size: 3340 931
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3788 3820 3788
Size: 32 4239
Tth111I GACn'n_nGTC
Cuts at: 1060 2068 1060
Size: 1008 3263
XbaI T'CTAG_A
Cuts at: 1302 1302
Size: 4271
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 191 213 779 1131 1766 191
Size: 22 566 352 635 2696
Fragments arranged by size:
2696 635 566 352 22
XmaI C'CCGG_G
Cuts at: 129 1291 129
Size: 1162 3109
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PspOMI PsrI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AloI AscI AvrII BaeI
BbsI BbvCI BcgI BfrBI BmgBI BmtI Bpu10I BsaXI
BsiWI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI
CspCI DraIII EcoRV FseI FspAI HpaI MfeI NcoI
NdeI NheI NruI NsiI PacI PmlI PsiI PspXI
SexAI SfiI SnaBI SrfI SspI SwaI XhoI XmnI
ZraI