Restriction map for
pF1KB6519
(Circular) (Six-base) MAPSORT of: pF1KB6519.seq Check: 6670 from: 1 to: 4199
pF1KB6519 4199 bp
With 182 enzymes: SgfI *
March 2, 2010 14:26 ..
AccI GT'mk_AC
Cuts at: 991 1237 991
Size: 246 3953
Acc65I G'GTAC_C
Cuts at: 1215 1215
Size: 4199
AclI AA'CG_TT
Cuts at: 4039 4039
Size: 4199
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 437 2024 2456 3203 3471 437
Size: 1587 432 747 268 1165
Fragments arranged by size:
1587 1165 747 432 268
AflIII A'CryG_T
Cuts at: 1551 2655 3632 1551
Size: 1104 977 2118
Fragments arranged by size:
2118 1104 977
AgeI A'CCGG_T
Cuts at: 2587 2587
Size: 4199
AlwNI CAG_nnn'CTG
Cuts at: 1504 3071 1504
Size: 1567 2632
ApaLI G'TGCA_C
Cuts at: 2969 2969
Size: 4199
ApoI r'AATT_y
Cuts at: 1203 3552 3808 1203
Size: 2349 256 1594
Fragments arranged by size:
2349 1594 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4199
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4199
AvaI C'yCGr_G
Cuts at: 506 1219 506
Size: 713 3486
BamHI G'GATC_C
Cuts at: 1224 1224
Size: 4199
BanI G'GyrC_C
Cuts at: 1215 1629 1877 1912 1215
Size: 414 248 35 3502
Fragments arranged by size:
3502 414 248 35
BanII G_rGCy'C
Cuts at: 279 350 507 1213 2243 279
Size: 71 157 706 1030 2235
Fragments arranged by size:
2235 1030 706 157 71
BbeI G_GCGC'C
Cuts at: 1633 1881 1633
Size: 248 3951
BbsI GAAGACnn'nnnn_
Cuts at: 1159 1159
Size: 4199
BciVI GTATCCnnnnn_n'
Cuts at: 993 2097 2858 4199 993
Size: 1104 761 1341 993
Fragments arranged by size:
1341 1104 993 761
BclI T'GATC_A
Cuts at: 1155 1719 1155
Size: 564 3635
BglI GCCn_nnn'nGGC
Cuts at: 131 232 131
Size: 101 4098
BglII A'GATC_T
Cuts at: 480 1714 3751 480
Size: 1234 2037 928
Fragments arranged by size:
2037 1234 928
BlpI GC'TnA_GC
Cuts at: 1311 1311
Size: 4199
Bme1580I G_kGCm'C
Cuts at: 1824 1917 2973 1824
Size: 93 1056 3050
Fragments arranged by size:
3050 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 270 1170 1411 1492 1827 3971 270
Size: 900 241 81 335 2144 498
Fragments arranged by size:
2144 900 498 335 241 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 222 2566 222
Size: 2344 1855
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 148 845 148
Size: 697 3502
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1375 2254 2746 3044 1375
Size: 879 492 298 2530
Fragments arranged by size:
2530 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3903 3903
Size: 4199
BsaAI yAC'GTr
Cuts at: 1552 1552
Size: 4199
BsaBI GATnn'nnATC
Cuts at: 6 896 1713 3750 6
Size: 890 817 2037 455
Fragments arranged by size:
2037 890 817 455
BsaHI Gr'CG_yC
Cuts at: 1630 1878 2580 4076 1630
Size: 248 702 1496 1753
Fragments arranged by size:
1753 1496 702 248
BsaWI w'CCGG_w
Cuts at: 300 1388 1909 2587 2861 3008 3419 300
Size: 1088 521 678 274 147 411 1080
Fragments arranged by size:
1088 1080 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 322 322
Size: 4199
BseYI C'CCAG_C
Cuts at: 1624 2382 2959 3455 1624
Size: 758 577 496 2368
Fragments arranged by size:
2368 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 212 1741 1787 2995 104
Size: 108 1529 46 1208 1308
Fragments arranged by size:
1529 1308 1208 108 46
BsiHKAI G_wGCw'C
Cuts at: 796 1213 1991 2181 2973 796
Size: 417 778 190 792 2022
Fragments arranged by size:
2022 792 778 417 190
Bsp1286I G_dGCh'C
Cuts at: 279 350 507 796 1213 1824 1917 1991 2181
Size: 71 157 289 417 611 93 74 190
Cuts at: 2181 2243 2973 279
Size: 62 730 1505
Fragments arranged by size:
1505 730 611 417 289 190 157 93
74 71 62
BspEI T'CCGG_A
Cuts at: 3419 3419
Size: 4199
BspHI T'CATG_A
Cuts at: 627 3375 627
Size: 2748 1451
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1249 1463 1765 2146 1249
Size: 214 302 381 3302
Fragments arranged by size:
3302 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2491 2545 4029 4194 50
Size: 2441 54 1484 165 55
Fragments arranged by size:
2441 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 981 2111 981
Size: 1130 3069
BsrFI r'CCGG_y
Cuts at: 2197 2378 2587 2197
Size: 181 209 3809
Fragments arranged by size:
3809 209 181
BsrGI T'GTAC_A
Cuts at: 533 533
Size: 4199
BssSI C'ACGA_G
Cuts at: 2470 2828 2470
Size: 358 3841
BstAPI GCAn_nnn'nTGC
Cuts at: 1576 1576
Size: 4199
BstBI TT'CG_AA
Cuts at: 2560 2560
Size: 4199
BstEII G'GTnAC_C
Cuts at: 915 915
Size: 4199
BstXI CCAn_nnnn'nTGG
Cuts at: 114 114
Size: 4199
BstYI r'GATC_y
Cuts at: 1 480 1224 1691 1714 2049 2295 3307 3393
Size: 479 744 467 23 335 246 1012 86
Cuts at: 3393 3405 3751 1
Size: 12 346 449
Fragments arranged by size:
1012 744 479 467 449 346 335 246
86 23 12
BstZ17I GTA'TAC
Cuts at: 992 992
Size: 4199
Bsu36I CC'TnA_GG
Cuts at: 167 167
Size: 4199
BtgI C'CryG_G
Cuts at: 107 107
Size: 4199
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 236 2149 2330 2493 236
Size: 1913 181 163 1942
Fragments arranged by size:
1942 1913 181 163
BtsI GCAGTG_nn'
Cuts at: 236 1442 236
Size: 1206 2993
DraI TTT'AAA
Cuts at: 842 1198 842
Size: 356 3843
DrdI GACnn_nn'nnGTC
Cuts at: 1905 2763 1905
Size: 858 3341
EaeI y'GGCC_r
Cuts at: 248 1784 1958 2349 2376 3721 248
Size: 1536 174 391 27 1345 726
Fragments arranged by size:
1536 1345 726 391 174 27
EagI C'GGCC_G
Cuts at: 1784 1784
Size: 4199
EarI CTCTTCn'nnn_
Cuts at: 149 589 1076 2222 2432 149
Size: 440 487 1146 210 1916
Fragments arranged by size:
1916 1146 487 440 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 163 2407 2717 2863 4009 163
Size: 2244 310 146 1146 353
Fragments arranged by size:
2244 1146 353 310 146
EcoICRI GAG'CTC
Cuts at: 1211 1211
Size: 4199
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 148 437 845 2024 2456 3203 3471 148
Size: 289 408 1179 432 747 268 876
Fragments arranged by size:
1179 876 747 432 408 289 268
EcoO109I rG'GnC_Cy
Cuts at: 1338 1338
Size: 4199
EcoRI G'AATT_C
Cuts at: 1203 1203
Size: 4199
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2218 2218
Size: 4199
FspI TGC'GCA
Cuts at: 1980 1980
Size: 4199
HaeII r_GCGC'y
Cuts at: 317 1633 1881 2903 3882 317
Size: 1316 248 1022 979 634
Fragments arranged by size:
1316 1022 979 634 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2041 2071 2073 2103 2041
Size: 30 2 30 4137
Fragments arranged by size:
4137 30 30 2
HincII GTy'rAC
Cuts at: 363 1238 363
Size: 875 3324
KasI G'GCGC_C
Cuts at: 1629 1877 1629
Size: 248 3951
KpnI G_GTAC'C
Cuts at: 1219 1219
Size: 4199
MluI A'CGCG_T
Cuts at: 3632 3632
Size: 4199
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2870 3054 2870
Size: 184 4015
MscI TGG'CCA
Cuts at: 1960 1960
Size: 4199
MslI CAynn'nnrTG
Cuts at: 746 2188 2315 746
Size: 1442 127 2630
Fragments arranged by size:
2630 1442 127
MspA1I CmG'CkG
Cuts at: 1310 1624 1984 2997 3242 1310
Size: 314 360 1013 245 2267
Fragments arranged by size:
2267 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2380 2380
Size: 4199
NarI GG'CG_CC
Cuts at: 1630 1878 1630
Size: 248 3951
NcoI C'CATG_G
Cuts at: 107 107
Size: 4199
NgoMIV G'CCGG_C
Cuts at: 2378 2378
Size: 4199
NspI r_CATG'y
Cuts at: 447 745 808 1252 1752 2659 447
Size: 298 63 444 500 907 1987
Fragments arranged by size:
1987 907 500 444 298 63
PciI A'CATG_T
Cuts at: 2655 2655
Size: 4199
PmeI GTTT'AAAC
Cuts at: 1198 1198
Size: 4199
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3338 3370 3338
Size: 32 4167
PspXI vC'TCGA_Gb
Cuts at: 506 506
Size: 4199
PstI C_TGCA'G
Cuts at: 1246 1931 1246
Size: 685 3514
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4199
PvuII CAG'CTG
Cuts at: 1624 1984 1624
Size: 360 3839
RsrII CG'GwC_CG
Cuts at: 2394 2394
Size: 4199
SacI G_AGCT'C
Cuts at: 1213 1213
Size: 4199
SalI G'TCGA_C
Cuts at: 1236 1236
Size: 4199
SapI GCTCTTCn'nnn_
Cuts at: 2222 2432 2222
Size: 210 3989
SbfI CC_TGCA'GG
Cuts at: 1246 1246
Size: 4199
ScaI AGT'ACT
Cuts at: 3716 3716
Size: 4199
SfcI C'TryA_G
Cuts at: 33 556 872 1242 1927 2920 3111 33
Size: 523 316 370 685 993 191 1121
Fragments arranged by size:
1121 993 685 523 370 316 191
SfoI GGC'GCC
Cuts at: 1631 1879 1631
Size: 248 3951
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4199
SmaI CCC'GGG
Cuts at: 1221 1221
Size: 4199
SmlI C'TyrA_G
Cuts at: 506 1354 2269 2761 3023 506
Size: 848 915 492 262 1682
Fragments arranged by size:
1682 915 848 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4199
SphI G_CATG'C
Cuts at: 808 1252 1752 808
Size: 444 500 3255
Fragments arranged by size:
3255 500 444
SspI AAT'ATT
Cuts at: 468 1061 1190 468
Size: 593 129 3477
Fragments arranged by size:
3477 593 129
StyI C'CwwG_G
Cuts at: 107 317 1333 1687 107
Size: 210 1016 354 2619
Fragments arranged by size:
2619 1016 354 210
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 198 1782 1915 2374 2583 198
Size: 1584 133 459 209 1814
Fragments arranged by size:
1814 1584 459 209 133
TatI w'GTAC_w
Cuts at: 533 3714 533
Size: 3181 1018
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3716 3748 3716
Size: 32 4167
Tth111I GACn'n_nGTC
Cuts at: 1996 1996
Size: 4199
XbaI T'CTAG_A
Cuts at: 1230 1230
Size: 4199
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 945 1694 945
Size: 749 3450
XhoI C'TCGA_G
Cuts at: 506 506
Size: 4199
XmaI C'CCGG_G
Cuts at: 1219 1219
Size: 4199
XmnI GAAnn'nnTTC
Cuts at: 566 566
Size: 4199
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PmeI PpiI PspXI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI
BmtI Bpu10I BsaXI BsgI BsiWI BsmI BsmBI BssHII
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
StuI SwaI ZraI