Restriction map for
pF1KB6520
(Circular) (Six-base) MAPSORT of: pF1KB6520.seq Check: 833 from: 1 to: 4271
pF1KB6520 4271 bp
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AatII G_ACGT'C
Cuts at: 998 998
Size: 4271
AccI GT'mk_AC
Cuts at: 1309 1309
Size: 4271
Acc65I G'GTAC_C
Cuts at: 380 881 1287 380
Size: 501 406 3364
Fragments arranged by size:
3364 501 406
AclI AA'CG_TT
Cuts at: 4111 4111
Size: 4271
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 781 2096 2528 3275 3543 781
Size: 1315 432 747 268 1509
Fragments arranged by size:
1509 1315 747 432 268
AflIII A'CryG_T
Cuts at: 1623 2727 3704 1623
Size: 1104 977 2190
Fragments arranged by size:
2190 1104 977
AgeI A'CCGG_T
Cuts at: 2659 2659
Size: 4271
AhdI GACnn_n'nnGTC
Cuts at: 618 673 618
Size: 55 4216
AleI CACnn'nnGTG
Cuts at: 860 860
Size: 4271
AlwNI CAG_nnn'CTG
Cuts at: 1109 1576 3143 1109
Size: 467 1567 2237
Fragments arranged by size:
2237 1567 467
ApaI G_GGCC'C
Cuts at: 944 944
Size: 4271
ApaLI G'TGCA_C
Cuts at: 508 3041 508
Size: 2533 1738
ApoI r'AATT_y
Cuts at: 1275 3624 3880 1275
Size: 2349 256 1666
Fragments arranged by size:
2349 1666 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4271
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4271
AvaI C'yCGr_G
Cuts at: 199 490 498 750 937 1291 199
Size: 291 8 252 187 354 3179
Fragments arranged by size:
3179 354 291 252 187 8
BamHI G'GATC_C
Cuts at: 169 1051 1296 169
Size: 882 245 3144
Fragments arranged by size:
3144 882 245
BanI G'GyrC_C
Cuts at: 380 835 881 1287 1701 1949 1984 380
Size: 455 46 406 414 248 35 2667
Fragments arranged by size:
2667 455 414 406 248 46 35
BanII G_rGCy'C
Cuts at: 146 182 944 1116 1165 1285 2315 146
Size: 36 762 172 49 120 1030 2102
Fragments arranged by size:
2102 1030 762 172 120 49 36
BbeI G_GCGC'C
Cuts at: 839 1705 1953 839
Size: 866 248 3157
Fragments arranged by size:
3157 866 248
BbsI GAAGACnn'nnnn_
Cuts at: 263 704 992 263
Size: 441 288 3542
Fragments arranged by size:
3542 441 288
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 440 474 440
Size: 34 4237
BciVI GTATCCnnnnn_n'
Cuts at: 2169 2930 4271 2169
Size: 761 1341 2169
Fragments arranged by size:
2169 1341 761
BclI T'GATC_A
Cuts at: 1791 1791
Size: 4271
BglI GCCn_nnn'nGGC
Cuts at: 112 485 112
Size: 373 3898
BglII A'GATC_T
Cuts at: 213 1786 3823 213
Size: 1573 2037 661
Fragments arranged by size:
2037 1573 661
BlpI GC'TnA_GC
Cuts at: 1383 1383
Size: 4271
Bme1580I G_kGCm'C
Cuts at: 512 944 1896 1989 3045 512
Size: 432 952 93 1056 1738
Fragments arranged by size:
1738 1056 952 432 93
BmgBI CAC'GTC
Cuts at: 156 156
Size: 4271
BmrI ACTGGGnnnn_n'
Cuts at: 534 1119 1483 1564 1899 4043 534
Size: 585 364 81 335 2144 762
Fragments arranged by size:
2144 762 585 364 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2638 2638
Size: 4271
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 268 1033 268
Size: 765 3506
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 168 583 655 1447 2326 2818 3116 168
Size: 415 72 792 879 492 298 1323
Fragments arranged by size:
1323 879 792 492 415 298 72
BsaI GGTCTCn'nnnn_
Cuts at: 3975 3975
Size: 4271
BsaAI yAC'GTr
Cuts at: 1624 1624
Size: 4271
BsaBI GATnn'nnATC
Cuts at: 6 1785 3822 6
Size: 1779 2037 455
Fragments arranged by size:
2037 1779 455
BsaHI Gr'CG_yC
Cuts at: 836 995 1702 1950 2652 4148 836
Size: 159 707 248 702 1496 959
Fragments arranged by size:
1496 959 707 702 248 159
BsaWI w'CCGG_w
Cuts at: 409 1460 1981 2659 2933 3080 3491 409
Size: 1051 521 678 274 147 411 1189
Fragments arranged by size:
1189 1051 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 678 820 678
Size: 142 4129
BseYI C'CCAG_C
Cuts at: 120 145 874 1089 1696 2454 3031 3527 120
Size: 25 729 215 607 758 577 496 864
Fragments arranged by size:
864 758 729 607 577 496 215 25
BsiEI CG_ry'CG
Cuts at: 104 615 1813 1859 3067 104
Size: 511 1198 46 1208 1308
Fragments arranged by size:
1308 1208 1198 511 46
BsiHKAI G_wGCw'C
Cuts at: 182 512 1165 1285 2063 2253 3045 182
Size: 330 653 120 778 190 792 1408
Fragments arranged by size:
1408 792 778 653 330 190 120
BsmI GAATG_Cn'
Cuts at: 1068 1068
Size: 4271
BsmBI CGTCTCn'nnnn_
Cuts at: 409 409
Size: 4271
Bsp1286I G_dGCh'C
Cuts at: 146 182 512 944 1116 1165 1285 1896 1989
Size: 36 330 432 172 49 120 611 93
Cuts at: 1989 2063 2253 2315 3045 146
Size: 74 190 62 730 1372
Fragments arranged by size:
1372 730 611 432 330 190 172 120
93 74 62 49 36
BspEI T'CCGG_A
Cuts at: 409 3491 409
Size: 3082 1189
BspHI T'CATG_A
Cuts at: 3447 3447
Size: 4271
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1321 1535 1837 2218 1321
Size: 214 302 381 3374
Fragments arranged by size:
3374 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2563 2617 4101 4266 50
Size: 2513 54 1484 165 55
Fragments arranged by size:
2513 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2183 2183
Size: 4271
BsrFI r'CCGG_y
Cuts at: 899 2269 2450 2659 899
Size: 1370 181 209 2511
Fragments arranged by size:
2511 1370 209 181
BssSI C'ACGA_G
Cuts at: 2542 2900 2542
Size: 358 3913
BstAPI GCAn_nnn'nTGC
Cuts at: 272 452 1648 272
Size: 180 1196 2895
Fragments arranged by size:
2895 1196 180
BstBI TT'CG_AA
Cuts at: 644 2632 644
Size: 1988 2283
BstXI CCAn_nnnn'nTGG
Cuts at: 1194 1194
Size: 4271
BstYI r'GATC_y
Cuts at: 1 169 213 320 1051 1296 1763 1786 2121
Size: 168 44 107 731 245 467 23 335
Cuts at: 2121 2367 3379 3465 3477 3823 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 731 467 449 346 335 246 245
168 107 86 44 23 12
BtgI C'CryG_G
Cuts at: 107 1044 107
Size: 937 3334
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2221 2402 2565 2221
Size: 181 163 3927
Fragments arranged by size:
3927 181 163
BtsI GCAGTG_nn'
Cuts at: 1514 1514
Size: 4271
DraI TTT'AAA
Cuts at: 1270 1270
Size: 4271
DrdI GACnn_nn'nnGTC
Cuts at: 994 1977 2835 994
Size: 983 858 2430
Fragments arranged by size:
2430 983 858
EaeI y'GGCC_r
Cuts at: 113 1175 1856 2030 2421 2448 3793 113
Size: 1062 681 174 391 27 1345 591
Fragments arranged by size:
1345 1062 681 591 391 174 27
EagI C'GGCC_G
Cuts at: 1856 1856
Size: 4271
EarI CTCTTCn'nnn_
Cuts at: 364 2294 2504 364
Size: 1930 210 2131
Fragments arranged by size:
2131 1930 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1064 2479 2789 2935 4081 1064
Size: 1415 310 146 1146 1254
Fragments arranged by size:
1415 1254 1146 310 146
EcoICRI GAG'CTC
Cuts at: 180 1163 1283 180
Size: 983 120 3168
Fragments arranged by size:
3168 983 120
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 268 781 1033 2096 2528 3275 3543 268
Size: 513 252 1063 432 747 268 996
Fragments arranged by size:
1063 996 747 513 432 268 252
EcoO109I rG'GnC_Cy
Cuts at: 494 814 941 1410 494
Size: 320 127 469 3355
Fragments arranged by size:
3355 469 320 127
EcoRI G'AATT_C
Cuts at: 1275 1275
Size: 4271
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1224 2290 1224
Size: 1066 3205
FspI TGC'GCA
Cuts at: 2052 2052
Size: 4271
HaeII r_GCGC'y
Cuts at: 839 1705 1953 2975 3954 839
Size: 866 248 1022 979 1156
Fragments arranged by size:
1156 1022 979 866 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 604 636 659 691 985 1017 2113 2143 2145
Size: 32 23 32 294 32 1096 30 2
Cuts at: 2145 2175 604
Size: 30 2700
Fragments arranged by size:
2700 1096 294 32 32 32 30 30
23 2
HincII GTy'rAC
Cuts at: 1310 1310
Size: 4271
KasI G'GCGC_C
Cuts at: 835 1701 1949 835
Size: 866 248 3157
Fragments arranged by size:
3157 866 248
KpnI G_GTAC'C
Cuts at: 384 885 1291 384
Size: 501 406 3364
Fragments arranged by size:
3364 501 406
MluI A'CGCG_T
Cuts at: 3704 3704
Size: 4271
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 556 602 2942 3126 556
Size: 46 2340 184 1701
Fragments arranged by size:
2340 1701 184 46
MscI TGG'CCA
Cuts at: 2032 2032
Size: 4271
MslI CAynn'nnrTG
Cuts at: 154 860 2260 2387 154
Size: 706 1400 127 2038
Fragments arranged by size:
2038 1400 706 127
MspA1I CmG'CkG
Cuts at: 878 1382 1696 2056 3069 3314 878
Size: 504 314 360 1013 245 1835
Fragments arranged by size:
1835 1013 504 360 314 245
NaeI GCC'GGC
Cuts at: 2452 2452
Size: 4271
NarI GG'CG_CC
Cuts at: 836 1702 1950 836
Size: 866 248 3157
Fragments arranged by size:
3157 866 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4271
NdeI CA'TA_TG
Cuts at: 225 225
Size: 4271
NgoMIV G'CCGG_C
Cuts at: 2450 2450
Size: 4271
NspI r_CATG'y
Cuts at: 769 1324 1824 2731 769
Size: 555 500 907 2309
Fragments arranged by size:
2309 907 555 500
PasI CC'CwG_GG
Cuts at: 849 849
Size: 4271
PciI A'CATG_T
Cuts at: 2727 2727
Size: 4271
PfoI T'CCnGG_A
Cuts at: 347 1010 347
Size: 663 3608
PmeI GTTT'AAAC
Cuts at: 1270 1270
Size: 4271
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3410 3442 3410
Size: 32 4239
PpuMI rG'GwC_Cy
Cuts at: 494 814 494
Size: 320 3951
PspOMI G'GGCC_C
Cuts at: 940 940
Size: 4271
PstI C_TGCA'G
Cuts at: 572 911 1318 2003 572
Size: 339 407 685 2840
Fragments arranged by size:
2840 685 407 339
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4271
PvuII CAG'CTG
Cuts at: 878 1696 2056 878
Size: 818 360 3093
Fragments arranged by size:
3093 818 360
RsrII CG'GwC_CG
Cuts at: 2466 2466
Size: 4271
SacI G_AGCT'C
Cuts at: 182 1165 1285 182
Size: 983 120 3168
Fragments arranged by size:
3168 983 120
SalI G'TCGA_C
Cuts at: 1308 1308
Size: 4271
SanDI GG'GwC_CC
Cuts at: 814 814
Size: 4271
SapI GCTCTTCn'nnn_
Cuts at: 2294 2504 2294
Size: 210 4061
SbfI CC_TGCA'GG
Cuts at: 1318 1318
Size: 4271
ScaI AGT'ACT
Cuts at: 3788 3788
Size: 4271
SfcI C'TryA_G
Cuts at: 33 568 907 1314 1999 2992 3183 33
Size: 535 339 407 685 993 191 1121
Fragments arranged by size:
1121 993 685 535 407 339 191
SfoI GGC'GCC
Cuts at: 837 1703 1951 837
Size: 866 248 3157
Fragments arranged by size:
3157 866 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4271
SmaI CCC'GGG
Cuts at: 201 492 752 939 1293 201
Size: 291 260 187 354 3179
Fragments arranged by size:
3179 354 291 260 187
SmlI C'TyrA_G
Cuts at: 183 598 634 1426 2341 2833 3095 183
Size: 415 36 792 915 492 262 1359
Fragments arranged by size:
1359 915 792 492 415 262 36
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4271
SphI G_CATG'C
Cuts at: 1324 1824 1324
Size: 500 3771
SspI AAT'ATT
Cuts at: 1228 1228
Size: 4271
StuI AGG'CCT
Cuts at: 604 604
Size: 4271
StyI C'CwwG_G
Cuts at: 107 1405 1759 107
Size: 1298 354 2619
Fragments arranged by size:
2619 1298 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 516 629 1854 1987 2446 2655 516
Size: 113 1225 133 459 209 2132
Fragments arranged by size:
2132 1225 459 209 133 113
TatI w'GTAC_w
Cuts at: 3786 3786
Size: 4271
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1036 1068 3788 3820 1036
Size: 32 2720 32 1487
Fragments arranged by size:
2720 1487 32 32
Tth111I GACn'n_nGTC
Cuts at: 2068 2068
Size: 4271
XbaI T'CTAG_A
Cuts at: 1302 1302
Size: 4271
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 123 846 1766 123
Size: 723 920 2628
Fragments arranged by size:
2628 920 723
XmaI C'CCGG_G
Cuts at: 199 490 750 937 1291 199
Size: 291 260 187 354 3179
Fragments arranged by size:
3179 354 291 260 187
ZraI GAC'GTC
Cuts at: 996 996
Size: 4271
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PasI
PciI PfoI PmeI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AlfI AloI AscI AvrII BaeI
BbvCI BfrBI BmtI Bpu10I BsaXI BsgI BsiWI BsrGI
BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NheI NotI
NruI NsiI PacI PflMI PmlI PshAI PsiI PspXI
PsrI SacII SexAI SfiI SgrAI SnaBI SrfI SwaI
XhoI XmnI