Restriction map for
pF1KB6522
(Circular) (Six-base) MAPSORT of: pF1KB6522.seq Check: 8735 from: 1 to: 4274
pF1KB6522 4274 bp
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AccI GT'mk_AC
Cuts at: 1312 1312
Size: 4274
Acc65I G'GTAC_C
Cuts at: 1290 1290
Size: 4274
AclI AA'CG_TT
Cuts at: 4114 4114
Size: 4274
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 108 1277 2099 2531 3278 3546 108
Size: 1169 822 432 747 268 836
Fragments arranged by size:
1169 836 822 747 432 268
AflIII A'CryG_T
Cuts at: 1626 2730 3707 1626
Size: 1104 977 2193
Fragments arranged by size:
2193 1104 977
AgeI A'CCGG_T
Cuts at: 2662 2662
Size: 4274
AlwNI CAG_nnn'CTG
Cuts at: 863 1033 1579 3146 863
Size: 170 546 1567 1991
Fragments arranged by size:
1991 1567 546 170
ApaLI G'TGCA_C
Cuts at: 3044 3044
Size: 4274
ApoI r'AATT_y
Cuts at: 292 307 475 530 708 734 1278 3627 3883
Size: 15 168 55 178 26 544 2349 256
Cuts at: 3883 292
Size: 683
Fragments arranged by size:
2349 683 544 256 178 168 55 26
15
AseI AT'TA_AT
Cuts at: 20 20
Size: 4274
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4274
AvaI C'yCGr_G
Cuts at: 1294 1294
Size: 4274
BamHI G'GATC_C
Cuts at: 1299 1299
Size: 4274
BanI G'GyrC_C
Cuts at: 1290 1704 1952 1987 1290
Size: 414 248 35 3577
Fragments arranged by size:
3577 414 248 35
BanII G_rGCy'C
Cuts at: 1288 2318 1288
Size: 1030 3244
BbeI G_GCGC'C
Cuts at: 1708 1956 1708
Size: 248 4026
BbsI GAAGACnn'nnnn_
Cuts at: 527 527
Size: 4274
BbvCI CC'TCA_GC
Cuts at: 1166 1166
Size: 4274
BciVI GTATCCnnnnn_n'
Cuts at: 2172 2933 4274 2172
Size: 761 1341 2172
Fragments arranged by size:
2172 1341 761
BclI T'GATC_A
Cuts at: 495 1794 495
Size: 1299 2975
BglII A'GATC_T
Cuts at: 1789 3826 1789
Size: 2037 2237
BlpI GC'TnA_GC
Cuts at: 592 1386 592
Size: 794 3480
Bme1580I G_kGCm'C
Cuts at: 1899 1992 3048 1899
Size: 93 1056 3125
Fragments arranged by size:
3125 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1486 1567 1902 4046 1486
Size: 81 335 2144 1714
Fragments arranged by size:
2144 1714 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2641 2641
Size: 4274
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 285 285
Size: 4274
Bpu10I CC'TnA_GC
Cuts at: 1166 1166
Size: 4274
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1450 2329 2821 3119 1450
Size: 879 492 298 2605
Fragments arranged by size:
2605 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 965 3978 965
Size: 3013 1261
BsaAI yAC'GTr
Cuts at: 260 930 1205 1627 260
Size: 670 275 422 2907
Fragments arranged by size:
2907 670 422 275
BsaBI GATnn'nnATC
Cuts at: 6 1034 1788 3825 6
Size: 1028 754 2037 455
Fragments arranged by size:
2037 1028 754 455
BsaHI Gr'CG_yC
Cuts at: 1705 1953 2655 4151 1705
Size: 248 702 1496 1828
Fragments arranged by size:
1828 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1463 1984 2662 2936 3083 3494 1463
Size: 521 678 274 147 411 2243
Fragments arranged by size:
2243 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 103 133 103
Size: 30 4244
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 653 673 1063 1183 653
Size: 20 390 120 3744
Fragments arranged by size:
3744 390 120 20
BseYI C'CCAG_C
Cuts at: 1699 2457 3034 3530 1699
Size: 758 577 496 2443
Fragments arranged by size:
2443 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 527 527
Size: 4274
BsiEI CG_ry'CG
Cuts at: 104 1816 1862 3070 104
Size: 1712 46 1208 1308
Fragments arranged by size:
1712 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1288 2066 2256 3048 1288
Size: 778 190 792 2514
Fragments arranged by size:
2514 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 315 315
Size: 4274
Bsp1286I G_dGCh'C
Cuts at: 1288 1899 1992 2066 2256 2318 3048 1288
Size: 611 93 74 190 62 730 2514
Fragments arranged by size:
2514 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3494 3494
Size: 4274
BspHI T'CATG_A
Cuts at: 3450 3450
Size: 4274
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1324 1538 1840 2221 1324
Size: 214 302 381 3377
Fragments arranged by size:
3377 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2566 2620 4104 4269 50
Size: 2516 54 1484 165 55
Fragments arranged by size:
2516 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 391 2186 391
Size: 1795 2479
BsrFI r'CCGG_y
Cuts at: 2272 2453 2662 2272
Size: 181 209 3884
Fragments arranged by size:
3884 209 181
BsrGI T'GTAC_A
Cuts at: 918 918
Size: 4274
BssSI C'ACGA_G
Cuts at: 284 2545 2903 284
Size: 2261 358 1655
Fragments arranged by size:
2261 1655 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1651 1651
Size: 4274
BstBI TT'CG_AA
Cuts at: 2635 2635
Size: 4274
BstYI r'GATC_y
Cuts at: 1 198 816 1299 1766 1789 2124 2370 3382
Size: 197 618 483 467 23 335 246 1012
Cuts at: 3382 3468 3480 3826 1
Size: 86 12 346 449
Fragments arranged by size:
1012 618 483 467 449 346 335 246
197 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4274
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2224 2405 2568 2224
Size: 181 163 3930
Fragments arranged by size:
3930 181 163
BtsI GCAGTG_nn'
Cuts at: 539 1517 539
Size: 978 3296
DraI TTT'AAA
Cuts at: 194 1273 194
Size: 1079 3195
DrdI GACnn_nn'nnGTC
Cuts at: 1980 2838 1980
Size: 858 3416
EaeI y'GGCC_r
Cuts at: 1859 2033 2424 2451 3796 1859
Size: 174 391 27 1345 2337
Fragments arranged by size:
2337 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1859 1859
Size: 4274
EarI CTCTTCn'nnn_
Cuts at: 208 567 806 963 2297 2507 208
Size: 359 239 157 1334 210 1975
Fragments arranged by size:
1975 1334 359 239 210 157
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2482 2792 2938 4084 2482
Size: 310 146 1146 2672
Fragments arranged by size:
2672 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1286 1286
Size: 4274
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 108 285 1277 2099 2531 3278 3546 108
Size: 177 992 822 432 747 268 836
Fragments arranged by size:
992 836 822 747 432 268 177
EcoO109I rG'GnC_Cy
Cuts at: 580 903 1413 580
Size: 323 510 3441
Fragments arranged by size:
3441 510 323
EcoRI G'AATT_C
Cuts at: 1278 1278
Size: 4274
EcoRV GAT'ATC
Cuts at: 328 1140 328
Size: 812 3462
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 219 355 2293 219
Size: 136 1938 2200
Fragments arranged by size:
2200 1938 136
FspI TGC'GCA
Cuts at: 2055 2055
Size: 4274
HaeII r_GCGC'y
Cuts at: 1708 1956 2978 3957 1708
Size: 248 1022 979 2025
Fragments arranged by size:
2025 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 103 135 312 344 709 741 2116 2146 2148
Size: 32 177 32 365 32 1375 30 2
Cuts at: 2148 2178 103
Size: 30 2199
Fragments arranged by size:
2199 1375 365 177 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1313 1313
Size: 4274
HindIII A'AGCT_T
Cuts at: 548 548
Size: 4274
KasI G'GCGC_C
Cuts at: 1704 1952 1704
Size: 248 4026
KpnI G_GTAC'C
Cuts at: 1294 1294
Size: 4274
MluI A'CGCG_T
Cuts at: 3707 3707
Size: 4274
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 526 2945 3129 526
Size: 2419 184 1671
Fragments arranged by size:
2419 1671 184
MscI TGG'CCA
Cuts at: 2035 2035
Size: 4274
MslI CAynn'nnrTG
Cuts at: 112 670 1184 2263 2390 112
Size: 558 514 1079 127 1996
Fragments arranged by size:
1996 1079 558 514 127
MspA1I CmG'CkG
Cuts at: 848 1385 1699 2059 3072 3317 848
Size: 537 314 360 1013 245 1805
Fragments arranged by size:
1805 1013 537 360 314 245
NaeI GCC'GGC
Cuts at: 2455 2455
Size: 4274
NarI GG'CG_CC
Cuts at: 1705 1953 1705
Size: 248 4026
NcoI C'CATG_G
Cuts at: 107 107
Size: 4274
NgoMIV G'CCGG_C
Cuts at: 2453 2453
Size: 4274
NspI r_CATG'y
Cuts at: 1327 1827 2734 1327
Size: 500 907 2867
Fragments arranged by size:
2867 907 500
PciI A'CATG_T
Cuts at: 2730 2730
Size: 4274
PmeI GTTT'AAAC
Cuts at: 1273 1273
Size: 4274
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3413 3445 3413
Size: 32 4242
PpuMI rG'GwC_Cy
Cuts at: 580 580
Size: 4274
PsiI TTA'TAA
Cuts at: 740 740
Size: 4274
PstI C_TGCA'G
Cuts at: 1321 2006 1321
Size: 685 3589
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4274
PvuII CAG'CTG
Cuts at: 848 1699 2059 848
Size: 851 360 3063
Fragments arranged by size:
3063 851 360
RsrII CG'GwC_CG
Cuts at: 2469 2469
Size: 4274
SacI G_AGCT'C
Cuts at: 1288 1288
Size: 4274
SalI G'TCGA_C
Cuts at: 1311 1311
Size: 4274
SapI GCTCTTCn'nnn_
Cuts at: 2297 2507 2297
Size: 210 4064
SbfI CC_TGCA'GG
Cuts at: 1321 1321
Size: 4274
ScaI AGT'ACT
Cuts at: 3791 3791
Size: 4274
SfcI C'TryA_G
Cuts at: 33 1317 2002 2995 3186 33
Size: 1284 685 993 191 1121
Fragments arranged by size:
1284 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1706 1954 1706
Size: 248 4026
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4274
SmaI CCC'GGG
Cuts at: 1296 1296
Size: 4274
SmlI C'TyrA_G
Cuts at: 1429 2344 2836 3098 1429
Size: 915 492 262 2605
Fragments arranged by size:
2605 915 492 262
SnaBI TAC'GTA
Cuts at: 260 260
Size: 4274
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4274
SphI G_CATG'C
Cuts at: 1327 1827 1327
Size: 500 3774
SspI AAT'ATT
Cuts at: 757 757
Size: 4274
StyI C'CwwG_G
Cuts at: 107 1408 1762 107
Size: 1301 354 2619
Fragments arranged by size:
2619 1301 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 943 1857 1990 2449 2658 943
Size: 914 133 459 209 2559
Fragments arranged by size:
2559 914 459 209 133
TatI w'GTAC_w
Cuts at: 918 3789 918
Size: 2871 1403
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3791 3823 3791
Size: 32 4242
Tth111I GACn'n_nGTC
Cuts at: 2071 2071
Size: 4274
XbaI T'CTAG_A
Cuts at: 1305 1305
Size: 4274
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1239 1769 1239
Size: 530 3744
XmaI C'CCGG_G
Cuts at: 1294 1294
Size: 4274
XmnI GAAnn'nnTTC
Cuts at: 121 121
Size: 4274
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PmeI PpiI PpuMI
PsiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI SspI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BcgI BfrBI BglI BmgBI
BmtI BsiWI BsmI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI CspCI DraIII EcoNI FseI FspAI HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmlI PshAI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SrfI StuI SwaI XhoI ZraI