Restriction map for
pF1KB6523
(Circular) (Six-base) MAPSORT of: pF1KB6523.seq Check: 9293 from: 1 to: 4205
pF1KB6523 4205 bp
With 182 enzymes: SgfI *
October 7, 2009 14:20 ..
AccI GT'mk_AC
Cuts at: 1148 1243 1148
Size: 95 4110
Acc65I G'GTAC_C
Cuts at: 1221 1221
Size: 4205
AclI AA'CG_TT
Cuts at: 4045 4045
Size: 4205
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 806 2030 2462 3209 3477 806
Size: 1224 432 747 268 1534
Fragments arranged by size:
1534 1224 747 432 268
AflIII A'CryG_T
Cuts at: 1032 1557 2661 3638 1032
Size: 525 1104 977 1599
Fragments arranged by size:
1599 1104 977 525
AgeI A'CCGG_T
Cuts at: 2593 2593
Size: 4205
AleI CACnn'nnGTG
Cuts at: 658 658
Size: 4205
AlwNI CAG_nnn'CTG
Cuts at: 1510 3077 1510
Size: 1567 2638
ApaI G_GGCC'C
Cuts at: 284 284
Size: 4205
ApaLI G'TGCA_C
Cuts at: 1036 2975 1036
Size: 1939 2266
ApoI r'AATT_y
Cuts at: 379 1209 3558 3814 379
Size: 830 2349 256 770
Fragments arranged by size:
2349 830 770 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4205
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4205
AvaI C'yCGr_G
Cuts at: 1225 1225
Size: 4205
BamHI G'GATC_C
Cuts at: 1230 1230
Size: 4205
BanI G'GyrC_C
Cuts at: 1221 1635 1883 1918 1221
Size: 414 248 35 3508
Fragments arranged by size:
3508 414 248 35
BanII G_rGCy'C
Cuts at: 245 284 1219 2249 245
Size: 39 935 1030 2201
Fragments arranged by size:
2201 1030 935 39
BbeI G_GCGC'C
Cuts at: 1639 1887 1639
Size: 248 3957
BbsI GAAGACnn'nnnn_
Cuts at: 817 817
Size: 4205
BciVI GTATCCnnnnn_n'
Cuts at: 2103 2864 4205 2103
Size: 761 1341 2103
Fragments arranged by size:
2103 1341 761
BclI T'GATC_A
Cuts at: 662 1725 662
Size: 1063 3142
BglII A'GATC_T
Cuts at: 606 1720 3757 606
Size: 1114 2037 1054
Fragments arranged by size:
2037 1114 1054
BlpI GC'TnA_GC
Cuts at: 1317 1317
Size: 4205
Bme1580I G_kGCm'C
Cuts at: 284 429 1040 1830 1923 2979 284
Size: 145 611 790 93 1056 1510
Fragments arranged by size:
1510 1056 790 611 145 93
BmrI ACTGGGnnnn_n'
Cuts at: 665 1417 1498 1833 3977 665
Size: 752 81 335 2144 893
Fragments arranged by size:
2144 893 752 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2572 2572
Size: 4205
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 148 910 1046 148
Size: 762 136 3307
Fragments arranged by size:
3307 762 136
Bpu10I CC'TnA_GC
Cuts at: 137 353 137
Size: 216 3989
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1075 1381 2260 2752 3050 1075
Size: 306 879 492 298 2230
Fragments arranged by size:
2230 879 492 306 298
BsaI GGTCTCn'nnnn_
Cuts at: 3909 3909
Size: 4205
BsaAI yAC'GTr
Cuts at: 1558 1558
Size: 4205
BsaBI GATnn'nnATC
Cuts at: 6 1719 3756 6
Size: 1713 2037 455
Fragments arranged by size:
2037 1713 455
BsaHI Gr'CG_yC
Cuts at: 1636 1884 2586 4082 1636
Size: 248 702 1496 1759
Fragments arranged by size:
1759 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1394 1915 2593 2867 3014 3425 1394
Size: 521 678 274 147 411 2174
Fragments arranged by size:
2174 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 727 757 1019 1049 727
Size: 30 262 30 3883
Fragments arranged by size:
3883 262 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 337 340 343 346 745 748 337
Size: 3 3 3 399 3 3794
Fragments arranged by size:
3794 399 3 3 3 3
BseYI C'CCAG_C
Cuts at: 150 271 1630 2388 2965 3461 150
Size: 121 1359 758 577 496 894
Fragments arranged by size:
1359 894 758 577 496 121
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1132 1132
Size: 4205
BsiEI CG_ry'CG
Cuts at: 104 1158 1747 1793 3001 104
Size: 1054 589 46 1208 1308
Fragments arranged by size:
1308 1208 1054 589 46
BsiHKAI G_wGCw'C
Cuts at: 1040 1219 1997 2187 2979 1040
Size: 179 778 190 792 2266
Fragments arranged by size:
2266 792 778 190 179
BsmBI CGTCTCn'nnnn_
Cuts at: 1115 1115
Size: 4205
Bsp1286I G_dGCh'C
Cuts at: 245 284 429 1040 1219 1830 1923 1997 2187
Size: 39 145 611 179 611 93 74 190
Cuts at: 2187 2249 2979 245
Size: 62 730 1471
Fragments arranged by size:
1471 730 611 611 190 179 145 93
74 62 39
BspEI T'CCGG_A
Cuts at: 3425 3425
Size: 4205
BspHI T'CATG_A
Cuts at: 3381 3381
Size: 4205
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1255 1469 1771 2152 1255
Size: 214 302 381 3308
Fragments arranged by size:
3308 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2497 2551 4035 4200 50
Size: 2447 54 1484 165 55
Fragments arranged by size:
2447 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 633 2117 633
Size: 1484 2721
BsrFI r'CCGG_y
Cuts at: 1153 2203 2384 2593 1153
Size: 1050 181 209 2765
Fragments arranged by size:
2765 1050 209 181
BssSI C'ACGA_G
Cuts at: 2476 2834 2476
Size: 358 3847
BstAPI GCAn_nnn'nTGC
Cuts at: 1582 1582
Size: 4205
BstBI TT'CG_AA
Cuts at: 2566 2566
Size: 4205
BstXI CCAn_nnnn'nTGG
Cuts at: 253 253
Size: 4205
BstYI r'GATC_y
Cuts at: 1 536 606 1051 1230 1697 1720 2055 2301
Size: 535 70 445 179 467 23 335 246
Cuts at: 2301 3313 3399 3411 3757 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 535 467 449 445 346 335 246
179 86 70 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2155 2336 2499 2155
Size: 181 163 3861
Fragments arranged by size:
3861 181 163
BtsI GCAGTG_nn'
Cuts at: 269 1448 269
Size: 1179 3026
DraI TTT'AAA
Cuts at: 1204 1204
Size: 4205
DraIII CAC_nnn'GTG
Cuts at: 1044 1044
Size: 4205
DrdI GACnn_nn'nnGTC
Cuts at: 1911 2769 1911
Size: 858 3347
EaeI y'GGCC_r
Cuts at: 746 1155 1790 1964 2355 2382 3727 746
Size: 409 635 174 391 27 1345 1224
Fragments arranged by size:
1345 1224 635 409 391 174 27
EagI C'GGCC_G
Cuts at: 1155 1790 1155
Size: 635 3570
EarI CTCTTCn'nnn_
Cuts at: 299 305 314 2228 2438 299
Size: 6 9 1914 210 2066
Fragments arranged by size:
2066 1914 210 9 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2413 2723 2869 4015 2413
Size: 310 146 1146 2603
Fragments arranged by size:
2603 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1217 1217
Size: 4205
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 148 806 910 1046 2030 2462 3209 3477 148
Size: 658 104 136 984 432 747 268 876
Fragments arranged by size:
984 876 747 658 432 268 136 104
EcoNI CCTnn'n_nnAGG
Cuts at: 892 892
Size: 4205
EcoO109I rG'GnC_Cy
Cuts at: 1344 1344
Size: 4205
EcoRI G'AATT_C
Cuts at: 1209 1209
Size: 4205
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 162 2224 162
Size: 2062 2143
FspI TGC'GCA
Cuts at: 652 1986 652
Size: 1334 2871
HaeII r_GCGC'y
Cuts at: 1639 1887 2909 3888 1639
Size: 248 1022 979 1956
Fragments arranged by size:
1956 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 727 759 911 943 2047 2077 2079 2109 727
Size: 32 152 32 1104 30 2 30 2823
Fragments arranged by size:
2823 1104 152 32 32 30 30 2
HincII GTy'rAC
Cuts at: 363 1244 363
Size: 881 3324
KasI G'GCGC_C
Cuts at: 1635 1883 1635
Size: 248 3957
KpnI G_GTAC'C
Cuts at: 1225 1225
Size: 4205
MluI A'CGCG_T
Cuts at: 3638 3638
Size: 4205
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 966 2876 3060 966
Size: 1910 184 2111
Fragments arranged by size:
2111 1910 184
MscI TGG'CCA
Cuts at: 1966 1966
Size: 4205
MslI CAynn'nnrTG
Cuts at: 658 2194 2321 658
Size: 1536 127 2542
Fragments arranged by size:
2542 1536 127
MspA1I CmG'CkG
Cuts at: 180 1316 1630 1990 3003 3248 180
Size: 1136 314 360 1013 245 1137
Fragments arranged by size:
1137 1136 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2386 2386
Size: 4205
NarI GG'CG_CC
Cuts at: 1636 1884 1636
Size: 248 3957
NgoMIV G'CCGG_C
Cuts at: 2384 2384
Size: 4205
NspI r_CATG'y
Cuts at: 1258 1758 2665 1258
Size: 500 907 2798
Fragments arranged by size:
2798 907 500
PciI A'CATG_T
Cuts at: 2661 2661
Size: 4205
PflMI CCAn_nnn'nTGG
Cuts at: 1107 1107
Size: 4205
PmeI GTTT'AAAC
Cuts at: 1204 1204
Size: 4205
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3344 3376 3344
Size: 32 4173
PspOMI G'GGCC_C
Cuts at: 280 280
Size: 4205
PstI C_TGCA'G
Cuts at: 1252 1937 1252
Size: 685 3520
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4205
PvuII CAG'CTG
Cuts at: 1630 1990 1630
Size: 360 3845
RsrII CG'GwC_CG
Cuts at: 2400 2400
Size: 4205
SacI G_AGCT'C
Cuts at: 1219 1219
Size: 4205
SalI G'TCGA_C
Cuts at: 1242 1242
Size: 4205
SapI GCTCTTCn'nnn_
Cuts at: 2228 2438 2228
Size: 210 3995
SbfI CC_TGCA'GG
Cuts at: 1252 1252
Size: 4205
ScaI AGT'ACT
Cuts at: 3722 3722
Size: 4205
SexAI A'CCwGG_T
Cuts at: 543 543
Size: 4205
SfcI C'TryA_G
Cuts at: 33 175 209 561 576 713 1248 1933 2926
Size: 142 34 352 15 137 535 685 993
Cuts at: 2926 3117 33
Size: 191 1121
Fragments arranged by size:
1121 993 685 535 352 191 142 137
34 15
SfoI GGC'GCC
Cuts at: 1637 1885 1637
Size: 248 3957
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4205
SmaI CCC'GGG
Cuts at: 1227 1227
Size: 4205
SmlI C'TyrA_G
Cuts at: 1090 1360 2275 2767 3029 1090
Size: 270 915 492 262 2266
Fragments arranged by size:
2266 915 492 270 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4205
SphI G_CATG'C
Cuts at: 1258 1758 1258
Size: 500 3705
StyI C'CwwG_G
Cuts at: 1339 1693 1339
Size: 354 3851
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1788 1921 2380 2589 1788
Size: 133 459 209 3404
Fragments arranged by size:
3404 459 209 133
TatI w'GTAC_w
Cuts at: 3720 3720
Size: 4205
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 487 519 533 565 1019 1051 3722 3754 487
Size: 32 14 32 454 32 2671 32 938
Fragments arranged by size:
2671 938 454 32 32 32 32 14
Tth111I GACn'n_nGTC
Cuts at: 2002 2002
Size: 4205
XbaI T'CTAG_A
Cuts at: 1188 1236 1188
Size: 48 4157
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1700 1700
Size: 4205
XmaI C'CCGG_G
Cuts at: 1225 1225
Size: 4205
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PciI PflMI PmeI PpiI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI
BsiWI BsmI BsrGI BssHII BstEII BstZ17I Bsu36I BtgI
ClaI CspCI EcoRV FseI FspAI HindIII HpaI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PfoI PmlI PpuMI PshAI PsiI PspXI PsrI SacII
SanDI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI