Restriction map for
pF1KB6529
(Circular) (Six-base) MAPSORT of: pF1KB6529.seq Check: 3765 from: 1 to: 4208
pF1KB6529 4208 bp
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AatII G_ACGT'C
Cuts at: 149 149
Size: 4208
AccI GT'mk_AC
Cuts at: 1246 1246
Size: 4208
Acc65I G'GTAC_C
Cuts at: 668 1224 668
Size: 556 3652
AclI AA'CG_TT
Cuts at: 4048 4048
Size: 4208
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 355 580 619 1137 2033 2465 3212 3480 355
Size: 225 39 518 896 432 747 268 1083
Fragments arranged by size:
1083 896 747 518 432 268 225 39
AflII C'TTAA_G
Cuts at: 520 520
Size: 4208
AflIII A'CryG_T
Cuts at: 182 1560 2664 3641 182
Size: 1378 1104 977 749
Fragments arranged by size:
1378 1104 977 749
AgeI A'CCGG_T
Cuts at: 2596 2596
Size: 4208
AhdI GACnn_n'nnGTC
Cuts at: 243 726 243
Size: 483 3725
AlwNI CAG_nnn'CTG
Cuts at: 495 813 855 891 1513 3080 495
Size: 318 42 36 622 1567 1623
Fragments arranged by size:
1623 1567 622 318 42 36
ApaLI G'TGCA_C
Cuts at: 547 2978 547
Size: 2431 1777
ApoI r'AATT_y
Cuts at: 1212 3561 3817 1212
Size: 2349 256 1603
Fragments arranged by size:
2349 1603 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4208
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4208
AvaI C'yCGr_G
Cuts at: 679 1228 679
Size: 549 3659
BamHI G'GATC_C
Cuts at: 1233 1233
Size: 4208
BanI G'GyrC_C
Cuts at: 668 828 1181 1224 1638 1886 1921 668
Size: 160 353 43 414 248 35 2955
Fragments arranged by size:
2955 414 353 248 160 43 35
BanII G_rGCy'C
Cuts at: 368 678 1222 2252 368
Size: 310 544 1030 2324
Fragments arranged by size:
2324 1030 544 310
BbeI G_GCGC'C
Cuts at: 1185 1642 1890 1185
Size: 457 248 3503
Fragments arranged by size:
3503 457 248
BbsI GAAGACnn'nnnn_
Cuts at: 369 369
Size: 4208
BbvCI CC'TCA_GC
Cuts at: 691 691
Size: 4208
BciVI GTATCCnnnnn_n'
Cuts at: 2106 2867 4208 2106
Size: 761 1341 2106
Fragments arranged by size:
2106 1341 761
BclI T'GATC_A
Cuts at: 1728 1728
Size: 4208
BglI GCCn_nnn'nGGC
Cuts at: 1009 1190 1009
Size: 181 4027
BglII A'GATC_T
Cuts at: 296 1723 3760 296
Size: 1427 2037 744
Fragments arranged by size:
2037 1427 744
BlpI GC'TnA_GC
Cuts at: 274 1320 274
Size: 1046 3162
Bme1580I G_kGCm'C
Cuts at: 551 1833 1926 2982 551
Size: 1282 93 1056 1777
Fragments arranged by size:
1777 1282 1056 93
BmgBI CAC'GTC
Cuts at: 198 984 198
Size: 786 3422
BmrI ACTGGGnnnn_n'
Cuts at: 1420 1501 1836 3980 1420
Size: 81 335 2144 1648
Fragments arranged by size:
2144 1648 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2575 2575
Size: 4208
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 982 994 982
Size: 12 4196
Bpu10I CC'TnA_GC
Cuts at: 691 691
Size: 4208
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1384 2263 2755 3053 1384
Size: 879 492 298 2539
Fragments arranged by size:
2539 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3912 3912
Size: 4208
BsaAI yAC'GTr
Cuts at: 183 654 1561 183
Size: 471 907 2830
Fragments arranged by size:
2830 907 471
BsaBI GATnn'nnATC
Cuts at: 6 1722 3759 6
Size: 1716 2037 455
Fragments arranged by size:
2037 1716 455
BsaHI Gr'CG_yC
Cuts at: 146 635 1182 1639 1887 2589 4085 146
Size: 489 547 457 248 702 1496 269
Fragments arranged by size:
1496 702 547 489 457 269 248
BsaWI w'CCGG_w
Cuts at: 119 1397 1918 2596 2870 3017 3428 119
Size: 1278 521 678 274 147 411 899
Fragments arranged by size:
1278 899 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 381 493 381
Size: 112 4096
BseYI C'CCAG_C
Cuts at: 384 538 1067 1633 2391 2968 3464 384
Size: 154 529 566 758 577 496 1128
Fragments arranged by size:
1128 758 577 566 529 496 154
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 868 868
Size: 4208
BsiEI CG_ry'CG
Cuts at: 104 1004 1750 1796 3004 104
Size: 900 746 46 1208 1308
Fragments arranged by size:
1308 1208 900 746 46
BsiHKAI G_wGCw'C
Cuts at: 229 551 1222 2000 2190 2982 229
Size: 322 671 778 190 792 1455
Fragments arranged by size:
1455 792 778 671 322 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1051 1051
Size: 4208
Bsp1286I G_dGCh'C
Cuts at: 229 368 551 678 1222 1833 1926 2000 2190
Size: 139 183 127 544 611 93 74 190
Cuts at: 2190 2252 2982 229
Size: 62 730 1455
Fragments arranged by size:
1455 730 611 544 190 183 139 127
93 74 62
BspEI T'CCGG_A
Cuts at: 119 3428 119
Size: 3309 899
BspHI T'CATG_A
Cuts at: 3384 3384
Size: 4208
BspMI ACCTGCnnnn'nnnn_
Cuts at: 965 1258 1472 1774 2155 965
Size: 293 214 302 381 3018
Fragments arranged by size:
3018 381 302 293 214
BsrBI CCG'CTC
Cuts at: 50 2500 2554 4038 4203 50
Size: 2450 54 1484 165 55
Fragments arranged by size:
2450 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1160 2120 1160
Size: 960 3248
BsrFI r'CCGG_y
Cuts at: 2206 2387 2596 2206
Size: 181 209 3818
Fragments arranged by size:
3818 209 181
BssHII G'CGCG_C
Cuts at: 309 309
Size: 4208
BssSI C'ACGA_G
Cuts at: 1096 2479 2837 1096
Size: 1383 358 2467
Fragments arranged by size:
2467 1383 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1585 1585
Size: 4208
BstBI TT'CG_AA
Cuts at: 2569 2569
Size: 4208
BstEII G'GTnAC_C
Cuts at: 657 657
Size: 4208
BstXI CCAn_nnnn'nTGG
Cuts at: 538 538
Size: 4208
BstYI r'GATC_y
Cuts at: 1 296 444 485 809 1233 1700 1723 2058
Size: 295 148 41 324 424 467 23 335
Cuts at: 2058 2304 3316 3402 3414 3760 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 467 449 424 346 335 324 295
246 148 86 41 23 12
Bsu36I CC'TnA_GG
Cuts at: 1049 1049
Size: 4208
BtgI C'CryG_G
Cuts at: 931 931
Size: 4208
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2158 2339 2502 2158
Size: 181 163 3864
Fragments arranged by size:
3864 181 163
BtsI GCAGTG_nn'
Cuts at: 1451 1451
Size: 4208
DraI TTT'AAA
Cuts at: 1207 1207
Size: 4208
DrdI GACnn_nn'nnGTC
Cuts at: 183 1914 2772 183
Size: 1731 858 1619
Fragments arranged by size:
1731 1619 858
EaeI y'GGCC_r
Cuts at: 230 260 869 1001 1793 1967 2358 2385 3730
Size: 30 609 132 792 174 391 27 1345
Cuts at: 3730 230
Size: 708
Fragments arranged by size:
1345 792 708 609 391 174 132 30
27
EagI C'GGCC_G
Cuts at: 1001 1793 1001
Size: 792 3416
EarI CTCTTCn'nnn_
Cuts at: 1049 2231 2441 1049
Size: 1182 210 2816
Fragments arranged by size:
2816 1182 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2416 2726 2872 4018 2416
Size: 310 146 1146 2606
Fragments arranged by size:
2606 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1220 1220
Size: 4208
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 355 580 619 982 994 1137 2033 2465 3212
Size: 225 39 363 12 143 896 432 747
Cuts at: 3212 3480 355
Size: 268 1083
Fragments arranged by size:
1083 896 747 432 363 268 225 143
39 12
EcoO109I rG'GnC_Cy
Cuts at: 209 555 1011 1044 1347 209
Size: 346 456 33 303 3070
Fragments arranged by size:
3070 456 346 303 33
EcoRI G'AATT_C
Cuts at: 1212 1212
Size: 4208
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2227 2227
Size: 4208
FspI TGC'GCA
Cuts at: 400 1989 400
Size: 1589 2619
HaeII r_GCGC'y
Cuts at: 996 1185 1642 1890 2912 3891 996
Size: 189 457 248 1022 979 1313
Fragments arranged by size:
1313 1022 979 457 248 189
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 229 261 712 744 2050 2080 2082 2112 229
Size: 32 451 32 1306 30 2 30 2325
Fragments arranged by size:
2325 1306 451 32 32 30 30 2
HincII GTy'rAC
Cuts at: 150 1247 150
Size: 1097 3111
KasI G'GCGC_C
Cuts at: 1181 1638 1886 1181
Size: 457 248 3503
Fragments arranged by size:
3503 457 248
KpnI G_GTAC'C
Cuts at: 672 1228 672
Size: 556 3652
MluI A'CGCG_T
Cuts at: 3641 3641
Size: 4208
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2879 3063 2879
Size: 184 4024
MscI TGG'CCA
Cuts at: 262 1969 262
Size: 1707 2501
MslI CAynn'nnrTG
Cuts at: 463 2197 2324 463
Size: 1734 127 2347
Fragments arranged by size:
2347 1734 127
MspA1I CmG'CkG
Cuts at: 206 665 695 801 924 1319 1633 1993 3006
Size: 459 30 106 123 395 314 360 1013
Cuts at: 3006 3251 206
Size: 245 1163
Fragments arranged by size:
1163 1013 459 395 360 314 245 123
106 30
NaeI GCC'GGC
Cuts at: 2389 2389
Size: 4208
NarI GG'CG_CC
Cuts at: 1182 1639 1887 1182
Size: 457 248 3503
Fragments arranged by size:
3503 457 248
NgoMIV G'CCGG_C
Cuts at: 2387 2387
Size: 4208
NspI r_CATG'y
Cuts at: 345 631 1261 1761 2668 345
Size: 286 630 500 907 1885
Fragments arranged by size:
1885 907 630 500 286
PciI A'CATG_T
Cuts at: 2664 2664
Size: 4208
PflMI CCAn_nnn'nTGG
Cuts at: 495 717 495
Size: 222 3986
PfoI T'CCnGG_A
Cuts at: 368 1040 368
Size: 672 3536
PmeI GTTT'AAAC
Cuts at: 1207 1207
Size: 4208
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3347 3379 3347
Size: 32 4176
PpuMI rG'GwC_Cy
Cuts at: 555 1044 555
Size: 489 3719
PshAI GACnn'nnGTC
Cuts at: 196 196
Size: 4208
PstI C_TGCA'G
Cuts at: 452 1255 1940 452
Size: 803 685 2720
Fragments arranged by size:
2720 803 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4208
PvuII CAG'CTG
Cuts at: 695 801 924 1633 1993 695
Size: 106 123 709 360 2910
Fragments arranged by size:
2910 709 360 123 106
RsrII CG'GwC_CG
Cuts at: 2403 2403
Size: 4208
SacI G_AGCT'C
Cuts at: 1222 1222
Size: 4208
SalI G'TCGA_C
Cuts at: 1245 1245
Size: 4208
SanDI GG'GwC_CC
Cuts at: 1044 1044
Size: 4208
SapI GCTCTTCn'nnn_
Cuts at: 2231 2441 2231
Size: 210 3998
SbfI CC_TGCA'GG
Cuts at: 1255 1255
Size: 4208
ScaI AGT'ACT
Cuts at: 3725 3725
Size: 4208
SexAI A'CCwGG_T
Cuts at: 419 494 419
Size: 75 4133
SfcI C'TryA_G
Cuts at: 33 448 1251 1936 2929 3120 33
Size: 415 803 685 993 191 1121
Fragments arranged by size:
1121 993 803 685 415 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 1009 1009
Size: 4208
SfoI GGC'GCC
Cuts at: 1183 1640 1888 1183
Size: 457 248 3503
Fragments arranged by size:
3503 457 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4208
SmaI CCC'GGG
Cuts at: 1230 1230
Size: 4208
SmlI C'TyrA_G
Cuts at: 520 1363 2278 2770 3032 520
Size: 843 915 492 262 1696
Fragments arranged by size:
1696 915 843 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4208
SphI G_CATG'C
Cuts at: 1261 1761 1261
Size: 500 3708
StuI AGG'CCT
Cuts at: 518 518
Size: 4208
StyI C'CwwG_G
Cuts at: 1342 1696 1342
Size: 354 3854
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1033 1791 1924 2383 2592 1033
Size: 758 133 459 209 2649
Fragments arranged by size:
2649 758 459 209 133
TatI w'GTAC_w
Cuts at: 524 3723 524
Size: 3199 1009
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3725 3757 3725
Size: 32 4176
Tth111I GACn'n_nGTC
Cuts at: 2005 2005
Size: 4208
XbaI T'CTAG_A
Cuts at: 1239 1239
Size: 4208
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1703 1703
Size: 4208
XmaI C'CCGG_G
Cuts at: 1228 1228
Size: 4208
XmnI GAAnn'nnTTC
Cuts at: 913 913
Size: 4208
ZraI GAC'GTC
Cuts at: 147 147
Size: 4208
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AleI AlfI AloI ApaI AscI AvrII
BaeI BcgI BfrBI BmtI BsaXI BsiWI BsmI BsrGI
BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NcoI NdeI NheI NotI NruI
NsiI PacI PasI PmlI PsiI PspOMI PspXI PsrI
SacII SgrAI SnaBI SrfI SspI SwaI XhoI