Restriction map for
pF1KB6531
(Circular) (Six-base) MAPSORT of: pF1KB6531.seq Check: 6548 from: 1 to: 4211
pF1KB6531 4211 bp
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AccI GT'mk_AC
Cuts at: 1249 1249
Size: 4211
Acc65I G'GTAC_C
Cuts at: 1227 1227
Size: 4211
AclI AA'CG_TT
Cuts at: 4051 4051
Size: 4211
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2036 2468 3215 3483 2036
Size: 432 747 268 2764
Fragments arranged by size:
2764 747 432 268
AflIII A'CryG_T
Cuts at: 1563 2667 3644 1563
Size: 1104 977 2130
Fragments arranged by size:
2130 1104 977
AgeI A'CCGG_T
Cuts at: 2599 2599
Size: 4211
AlwNI CAG_nnn'CTG
Cuts at: 496 606 1083 1516 3083 496
Size: 110 477 433 1567 1624
Fragments arranged by size:
1624 1567 477 433 110
ApaI G_GGCC'C
Cuts at: 340 889 982 340
Size: 549 93 3569
Fragments arranged by size:
3569 549 93
ApaLI G'TGCA_C
Cuts at: 781 2981 781
Size: 2200 2011
ApoI r'AATT_y
Cuts at: 679 915 1215 3564 3820 679
Size: 236 300 2349 256 1070
Fragments arranged by size:
2349 1070 300 256 236
AseI AT'TA_AT
Cuts at: 20 20
Size: 4211
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4211
AvaI C'yCGr_G
Cuts at: 803 1231 803
Size: 428 3783
BamHI G'GATC_C
Cuts at: 1236 1236
Size: 4211
BanI G'GyrC_C
Cuts at: 789 970 1227 1641 1889 1924 789
Size: 181 257 414 248 35 3076
Fragments arranged by size:
3076 414 257 248 181 35
BanII G_rGCy'C
Cuts at: 340 535 859 889 964 982 1010 1225 2255
Size: 195 324 30 75 18 28 215 1030
Cuts at: 2255 340
Size: 2296
Fragments arranged by size:
2296 1030 324 215 195 75 30 28
18
BbeI G_GCGC'C
Cuts at: 1645 1893 1645
Size: 248 3963
BbsI GAAGACnn'nnnn_
Cuts at: 244 865 244
Size: 621 3590
BbvCI CC'TCA_GC
Cuts at: 371 566 371
Size: 195 4016
BciVI GTATCCnnnnn_n'
Cuts at: 2109 2870 4211 2109
Size: 761 1341 2109
Fragments arranged by size:
2109 1341 761
BclI T'GATC_A
Cuts at: 1731 1731
Size: 4211
BglII A'GATC_T
Cuts at: 1726 3763 1726
Size: 2037 2174
BlpI GC'TnA_GC
Cuts at: 1057 1323 1057
Size: 266 3945
Bme1580I G_kGCm'C
Cuts at: 340 785 889 975 982 1173 1836 1929 2985
Size: 445 104 86 7 191 663 93 1056
Cuts at: 2985 340
Size: 1566
Fragments arranged by size:
1566 1056 663 445 191 104 93 86
7
BmgBI CAC'GTC
Cuts at: 1032 1032
Size: 4211
BmrI ACTGGGnnnn_n'
Cuts at: 499 1423 1504 1839 3983 499
Size: 924 81 335 2144 727
Fragments arranged by size:
2144 924 727 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 144 2578 144
Size: 2434 1777
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 430 442 430
Size: 12 4199
Bpu10I CC'TnA_GC
Cuts at: 371 566 371
Size: 195 4016
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1168 1387 2266 2758 3056 1168
Size: 219 879 492 298 2323
Fragments arranged by size:
2323 879 492 298 219
BsaI GGTCTCn'nnnn_
Cuts at: 3915 3915
Size: 4211
BsaAI yAC'GTr
Cuts at: 1564 1564
Size: 4211
BsaBI GATnn'nnATC
Cuts at: 6 1725 3762 6
Size: 1719 2037 455
Fragments arranged by size:
2037 1719 455
BsaHI Gr'CG_yC
Cuts at: 241 1642 1890 2592 4088 241
Size: 1401 248 702 1496 364
Fragments arranged by size:
1496 1401 702 364 248
BsaWI w'CCGG_w
Cuts at: 474 1400 1921 2599 2873 3020 3431 474
Size: 926 521 678 274 147 411 1254
Fragments arranged by size:
1254 926 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 826 856 826
Size: 30 4181
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 133 1030 1066 133
Size: 897 36 3278
Fragments arranged by size:
3278 897 36
BseYI C'CCAG_C
Cuts at: 340 1100 1636 2394 2971 3467 340
Size: 760 536 758 577 496 1084
Fragments arranged by size:
1084 760 758 577 536 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 309 634 309
Size: 325 3886
BsiEI CG_ry'CG
Cuts at: 104 1753 1799 3007 104
Size: 1649 46 1208 1308
Fragments arranged by size:
1649 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 597 785 1010 1225 2003 2193 2985 597
Size: 188 225 215 778 190 792 1823
Fragments arranged by size:
1823 792 778 225 215 190 188
BsmI GAATG_Cn'
Cuts at: 759 759
Size: 4211
Bsp1286I G_dGCh'C
Cuts at: 340 535 597 785 859 889 964 975 982
Size: 195 62 188 74 30 75 11 7
Cuts at: 982 1010 1173 1225 1836 1929 2003 2193 2255
Size: 28 163 52 611 93 74 190 62
Cuts at: 2255 2985 340
Size: 730 1566
Fragments arranged by size:
1566 730 611 195 190 188 163 93
75 74 74 62 62 52 30 28
11 7
BspEI T'CCGG_A
Cuts at: 3431 3431
Size: 4211
BspHI T'CATG_A
Cuts at: 3387 3387
Size: 4211
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1261 1475 1777 2158 1261
Size: 214 302 381 3314
Fragments arranged by size:
3314 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2503 2557 4041 4206 50
Size: 2453 54 1484 165 55
Fragments arranged by size:
2453 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 819 2123 819
Size: 1304 2907
BsrFI r'CCGG_y
Cuts at: 414 1104 2209 2390 2599 414
Size: 690 1105 181 209 2026
Fragments arranged by size:
2026 1105 690 209 181
BssSI C'ACGA_G
Cuts at: 2482 2840 2482
Size: 358 3853
BstAPI GCAn_nnn'nTGC
Cuts at: 496 789 1588 496
Size: 293 799 3119
Fragments arranged by size:
3119 799 293
BstBI TT'CG_AA
Cuts at: 876 2572 876
Size: 1696 2515
BstEII G'GTnAC_C
Cuts at: 303 303
Size: 4211
BstXI CCAn_nnnn'nTGG
Cuts at: 658 741 852 658
Size: 83 111 4017
Fragments arranged by size:
4017 111 83
BstYI r'GATC_y
Cuts at: 1 932 1236 1703 1726 2061 2307 3319 3405
Size: 931 304 467 23 335 246 1012 86
Cuts at: 3405 3417 3763 1
Size: 12 346 449
Fragments arranged by size:
1012 931 467 449 346 335 304 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 464 464
Size: 4211
BtgI C'CryG_G
Cuts at: 140 539 1149 140
Size: 399 610 3202
Fragments arranged by size:
3202 610 399
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2161 2342 2505 2161
Size: 181 163 3867
Fragments arranged by size:
3867 181 163
BtsI GCAGTG_nn'
Cuts at: 457 1454 457
Size: 997 3214
DraI TTT'AAA
Cuts at: 1210 1210
Size: 4211
DraIII CAC_nnn'GTG
Cuts at: 789 1115 789
Size: 326 3885
DrdI GACnn_nn'nnGTC
Cuts at: 654 1917 2775 654
Size: 1263 858 2090
Fragments arranged by size:
2090 1263 858
EaeI y'GGCC_r
Cuts at: 176 761 1106 1796 1970 2361 2388 3733 176
Size: 585 345 690 174 391 27 1345 654
Fragments arranged by size:
1345 690 654 585 391 345 174 27
EagI C'GGCC_G
Cuts at: 1796 1796
Size: 4211
EarI CTCTTCn'nnn_
Cuts at: 110 459 2234 2444 110
Size: 349 1775 210 1877
Fragments arranged by size:
1877 1775 349 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2419 2729 2875 4021 2419
Size: 310 146 1146 2609
Fragments arranged by size:
2609 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1008 1223 1008
Size: 215 3996
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 430 442 2036 2468 3215 3483 430
Size: 12 1594 432 747 268 1158
Fragments arranged by size:
1594 1158 747 432 268 12
EcoNI CCTnn'n_nnAGG
Cuts at: 211 211
Size: 4211
EcoO109I rG'GnC_Cy
Cuts at: 336 337 885 979 1350 336
Size: 1 548 94 371 3197
Fragments arranged by size:
3197 548 371 94 1
EcoRI G'AATT_C
Cuts at: 679 1215 679
Size: 536 3675
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2230 2230
Size: 4211
FspI TGC'GCA
Cuts at: 1992 1992
Size: 4211
HaeII r_GCGC'y
Cuts at: 1645 1893 2915 3894 1645
Size: 248 1022 979 1962
Fragments arranged by size:
1962 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 826 858 917 949 2053 2083 2085 2115 826
Size: 32 59 32 1104 30 2 30 2922
Fragments arranged by size:
2922 1104 59 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1250 1250
Size: 4211
KasI G'GCGC_C
Cuts at: 1641 1889 1641
Size: 248 3963
KpnI G_GTAC'C
Cuts at: 1231 1231
Size: 4211
MluI A'CGCG_T
Cuts at: 3644 3644
Size: 4211
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2882 3066 2882
Size: 184 4027
MscI TGG'CCA
Cuts at: 1972 1972
Size: 4211
MslI CAynn'nnrTG
Cuts at: 850 2200 2327 850
Size: 1350 127 2734
Fragments arranged by size:
2734 1350 127
MspA1I CmG'CkG
Cuts at: 323 370 496 1322 1636 1996 3009 3254 323
Size: 47 126 826 314 360 1013 245 1280
Fragments arranged by size:
1280 1013 826 360 314 245 126 47
NaeI GCC'GGC
Cuts at: 416 1106 2392 416
Size: 690 1286 2235
Fragments arranged by size:
2235 1286 690
NarI GG'CG_CC
Cuts at: 1642 1890 1642
Size: 248 3963
NcoI C'CATG_G
Cuts at: 539 539
Size: 4211
NgoMIV G'CCGG_C
Cuts at: 414 1104 2390 414
Size: 690 1286 2235
Fragments arranged by size:
2235 1286 690
NspI r_CATG'y
Cuts at: 408 1264 1764 2671 408
Size: 856 500 907 1948
Fragments arranged by size:
1948 907 856 500
PasI CC'CwG_GG
Cuts at: 957 966 975 957
Size: 9 9 4193
Fragments arranged by size:
4193 9 9
PciI A'CATG_T
Cuts at: 2667 2667
Size: 4211
PflMI CCAn_nnn'nTGG
Cuts at: 1083 1083
Size: 4211
PmeI GTTT'AAAC
Cuts at: 1210 1210
Size: 4211
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3350 3382 3350
Size: 32 4179
PspOMI G'GGCC_C
Cuts at: 336 885 978 336
Size: 549 93 3569
Fragments arranged by size:
3569 549 93
PstI C_TGCA'G
Cuts at: 215 492 551 602 1178 1258 1943 215
Size: 277 59 51 576 80 685 2483
Fragments arranged by size:
2483 685 576 277 80 59 51
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4211
PvuII CAG'CTG
Cuts at: 323 370 496 1636 1996 323
Size: 47 126 1140 360 2538
Fragments arranged by size:
2538 1140 360 126 47
RsrII CG'GwC_CG
Cuts at: 2406 2406
Size: 4211
SacI G_AGCT'C
Cuts at: 1010 1225 1010
Size: 215 3996
SalI G'TCGA_C
Cuts at: 1248 1248
Size: 4211
SapI GCTCTTCn'nnn_
Cuts at: 2234 2444 2234
Size: 210 4001
SbfI CC_TGCA'GG
Cuts at: 1258 1258
Size: 4211
ScaI AGT'ACT
Cuts at: 3728 3728
Size: 4211
SfcI C'TryA_G
Cuts at: 33 211 488 515 547 598 1174 1254 1939
Size: 178 277 27 32 51 576 80 685
Cuts at: 1939 2932 3123 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 685 576 277 191 178 80
51 32 27
SfoI GGC'GCC
Cuts at: 1643 1891 1643
Size: 248 3963
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4211
SmaI CCC'GGG
Cuts at: 805 1233 805
Size: 428 3783
SmlI C'TyrA_G
Cuts at: 1183 1366 2281 2773 3035 1183
Size: 183 915 492 262 2359
Fragments arranged by size:
2359 915 492 262 183
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4211
SphI G_CATG'C
Cuts at: 408 1264 1764 408
Size: 856 500 2855
Fragments arranged by size:
2855 856 500
StuI AGG'CCT
Cuts at: 1024 1024
Size: 4211
StyI C'CwwG_G
Cuts at: 539 1345 1699 539
Size: 806 354 3051
Fragments arranged by size:
3051 806 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 958 1114 1794 1927 2386 2595 958
Size: 156 680 133 459 209 2574
Fragments arranged by size:
2574 680 459 209 156 133
TatI w'GTAC_w
Cuts at: 638 3726 638
Size: 3088 1123
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3728 3760 3728
Size: 32 4179
Tth111I GACn'n_nGTC
Cuts at: 2008 2008
Size: 4211
XbaI T'CTAG_A
Cuts at: 1242 1242
Size: 4211
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 844 896 1706 844
Size: 52 810 3349
Fragments arranged by size:
3349 810 52
XmaI C'CCGG_G
Cuts at: 803 1231 803
Size: 428 3783
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PflMI PmeI PpiI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BcgI BfrBI BglI BmtI BsiWI
BsmBI BsrGI BssHII BstZ17I ClaI CspCI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PfoI PmlI PpuMI PshAI PsiI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI XmnI ZraI