Restriction map for
pF1KB6535
(Circular) (Six-base) MAPSORT of: pF1KB6535.seq Check: 7688 from: 1 to: 4214
pF1KB6535 4214 bp
With 182 enzymes: SgfI *
October 7, 2009 14:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 379 379
Size: 4214
AccI GT'mk_AC
Cuts at: 491 1252 491
Size: 761 3453
Acc65I G'GTAC_C
Cuts at: 1230 1230
Size: 4214
AclI AA'CG_TT
Cuts at: 4054 4054
Size: 4214
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 442 2039 2471 3218 3486 442
Size: 1597 432 747 268 1170
Fragments arranged by size:
1597 1170 747 432 268
AflIII A'CryG_T
Cuts at: 1566 2670 3647 1566
Size: 1104 977 2133
Fragments arranged by size:
2133 1104 977
AgeI A'CCGG_T
Cuts at: 2602 2602
Size: 4214
AleI CACnn'nnGTG
Cuts at: 394 583 919 1132 394
Size: 189 336 213 3476
Fragments arranged by size:
3476 336 213 189
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 861 1150 861
Size: 289 3925
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 622 654 622
Size: 32 4182
AlwNI CAG_nnn'CTG
Cuts at: 217 718 1519 3086 217
Size: 501 801 1567 1345
Fragments arranged by size:
1567 1345 801 501
ApaLI G'TGCA_C
Cuts at: 589 2984 589
Size: 2395 1819
ApoI r'AATT_y
Cuts at: 659 1218 3567 3823 659
Size: 559 2349 256 1050
Fragments arranged by size:
2349 1050 559 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4214
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4214
AvaI C'yCGr_G
Cuts at: 665 1234 665
Size: 569 3645
BamHI G'GATC_C
Cuts at: 1239 1239
Size: 4214
BanI G'GyrC_C
Cuts at: 895 1230 1644 1892 1927 895
Size: 335 414 248 35 3182
Fragments arranged by size:
3182 414 335 248 35
BanII G_rGCy'C
Cuts at: 672 877 1228 2258 672
Size: 205 351 1030 2628
Fragments arranged by size:
2628 1030 351 205
BbeI G_GCGC'C
Cuts at: 1648 1896 1648
Size: 248 3966
BbvCI CC'TCA_GC
Cuts at: 317 317
Size: 4214
BciVI GTATCCnnnnn_n'
Cuts at: 265 2112 2873 4214 265
Size: 1847 761 1341 265
Fragments arranged by size:
1847 1341 761 265
BclI T'GATC_A
Cuts at: 1734 1734
Size: 4214
BglI GCCn_nnn'nGGC
Cuts at: 751 751
Size: 4214
BglII A'GATC_T
Cuts at: 1729 3766 1729
Size: 2037 2177
BlpI GC'TnA_GC
Cuts at: 140 671 1326 140
Size: 531 655 3028
Fragments arranged by size:
3028 655 531
Bme1580I G_kGCm'C
Cuts at: 593 683 1839 1932 2988 593
Size: 90 1156 93 1056 1819
Fragments arranged by size:
1819 1156 1056 93 90
BmgBI CAC'GTC
Cuts at: 531 531
Size: 4214
BmrI ACTGGGnnnn_n'
Cuts at: 1426 1507 1842 3986 1426
Size: 81 335 2144 1654
Fragments arranged by size:
2144 1654 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2581 2581
Size: 4214
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 673 959 1004 673
Size: 286 45 3883
Fragments arranged by size:
3883 286 45
Bpu10I CC'TnA_GC
Cuts at: 317 317
Size: 4214
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1390 2269 2761 3059 1390
Size: 879 492 298 2545
Fragments arranged by size:
2545 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1083 3918 1083
Size: 2835 1379
BsaAI yAC'GTr
Cuts at: 1567 1567
Size: 4214
BsaBI GATnn'nnATC
Cuts at: 6 1728 3765 6
Size: 1722 2037 455
Fragments arranged by size:
2037 1722 455
BsaHI Gr'CG_yC
Cuts at: 1645 1893 2595 4091 1645
Size: 248 702 1496 1768
Fragments arranged by size:
1768 1496 702 248
BsaWI w'CCGG_w
Cuts at: 328 1403 1924 2602 2876 3023 3434 328
Size: 1075 521 678 274 147 411 1108
Fragments arranged by size:
1108 1075 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 622 652 953 983 622
Size: 30 301 30 3853
Fragments arranged by size:
3853 301 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1007 1007
Size: 4214
BseYI C'CCAG_C
Cuts at: 1159 1639 2397 2974 3470 1159
Size: 480 758 577 496 1903
Fragments arranged by size:
1903 758 577 496 480
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1156 1156
Size: 4214
BsiEI CG_ry'CG
Cuts at: 104 1756 1802 3010 104
Size: 1652 46 1208 1308
Fragments arranged by size:
1652 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 593 877 1228 2006 2196 2988 593
Size: 284 351 778 190 792 1819
Fragments arranged by size:
1819 792 778 351 284 190
Bsp1286I G_dGCh'C
Cuts at: 593 672 683 877 1228 1839 1932 2006 2196
Size: 79 11 194 351 611 93 74 190
Cuts at: 2196 2258 2988 593
Size: 62 730 1819
Fragments arranged by size:
1819 730 611 351 194 190 93 79
74 62 11
BspEI T'CCGG_A
Cuts at: 328 3434 328
Size: 3106 1108
BspHI T'CATG_A
Cuts at: 3390 3390
Size: 4214
BspMI ACCTGCnnnn'nnnn_
Cuts at: 379 776 853 1061 1264 1478 1780 2161 379
Size: 397 77 208 203 214 302 381 2432
Fragments arranged by size:
2432 397 381 302 214 208 203 77
BsrBI CCG'CTC
Cuts at: 50 2506 2560 4044 4209 50
Size: 2456 54 1484 165 55
Fragments arranged by size:
2456 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2126 2126
Size: 4214
BsrFI r'CCGG_y
Cuts at: 2212 2393 2602 2212
Size: 181 209 3824
Fragments arranged by size:
3824 209 181
BssSI C'ACGA_G
Cuts at: 2485 2843 2485
Size: 358 3856
BstAPI GCAn_nnn'nTGC
Cuts at: 542 835 1591 542
Size: 293 756 3165
Fragments arranged by size:
3165 756 293
BstBI TT'CG_AA
Cuts at: 2575 2575
Size: 4214
BstEII G'GTnAC_C
Cuts at: 1088 1088
Size: 4214
BstXI CCAn_nnnn'nTGG
Cuts at: 396 921 396
Size: 525 3689
BstYI r'GATC_y
Cuts at: 1 1239 1706 1729 2064 2310 3322 3408 3420
Size: 1238 467 23 335 246 1012 86 12
Cuts at: 3420 3766 1
Size: 346 449
Fragments arranged by size:
1238 1012 467 449 346 335 246 86
23 12
BstZ17I GTA'TAC
Cuts at: 492 492
Size: 4214
Bsu36I CC'TnA_GG
Cuts at: 1095 1095
Size: 4214
BtgI C'CryG_G
Cuts at: 485 920 485
Size: 435 3779
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 218 2164 2345 2508 218
Size: 1946 181 163 1924
Fragments arranged by size:
1946 1924 181 163
BtsI GCAGTG_nn'
Cuts at: 845 1457 845
Size: 612 3602
DraI TTT'AAA
Cuts at: 1213 1213
Size: 4214
DraIII CAC_nnn'GTG
Cuts at: 811 811
Size: 4214
DrdI GACnn_nn'nnGTC
Cuts at: 1920 2778 1920
Size: 858 3356
EaeI y'GGCC_r
Cuts at: 611 1799 1973 2364 2391 3736 611
Size: 1188 174 391 27 1345 1089
Fragments arranged by size:
1345 1188 1089 391 174 27
EagI C'GGCC_G
Cuts at: 1799 1799
Size: 4214
EarI CTCTTCn'nnn_
Cuts at: 2237 2447 2237
Size: 210 4004
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2422 2732 2878 4024 2422
Size: 310 146 1146 2612
Fragments arranged by size:
2612 1146 310 146
EcoICRI GAG'CTC
Cuts at: 875 1226 875
Size: 351 3863
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 442 673 959 1004 2039 2471 3218 3486 442
Size: 231 286 45 1035 432 747 268 1170
Fragments arranged by size:
1170 1035 747 432 286 268 231 45
EcoO109I rG'GnC_Cy
Cuts at: 1353 1353
Size: 4214
EcoRI G'AATT_C
Cuts at: 1218 1218
Size: 4214
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2233 2233
Size: 4214
FspI TGC'GCA
Cuts at: 535 1995 535
Size: 1460 2754
HaeII r_GCGC'y
Cuts at: 1648 1896 2918 3897 1648
Size: 248 1022 979 1965
Fragments arranged by size:
1965 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2056 2086 2088 2118 2056
Size: 30 2 30 4152
Fragments arranged by size:
4152 30 30 2
HincII GTy'rAC
Cuts at: 1253 1253
Size: 4214
HindIII A'AGCT_T
Cuts at: 817 817
Size: 4214
KasI G'GCGC_C
Cuts at: 1644 1892 1644
Size: 248 3966
KpnI G_GTAC'C
Cuts at: 1234 1234
Size: 4214
MluI A'CGCG_T
Cuts at: 3647 3647
Size: 4214
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2885 3069 2885
Size: 184 4030
MscI TGG'CCA
Cuts at: 1975 1975
Size: 4214
MslI CAynn'nnrTG
Cuts at: 394 583 919 1066 1072 1132 2203 2330 394
Size: 189 336 147 6 60 1071 127 2278
Fragments arranged by size:
2278 1071 336 189 147 127 60 6
MspA1I CmG'CkG
Cuts at: 420 459 570 651 979 1325 1639 1999 3012
Size: 39 111 81 328 346 314 360 1013
Cuts at: 3012 3257 420
Size: 245 1377
Fragments arranged by size:
1377 1013 360 346 328 314 245 111
81 39
NaeI GCC'GGC
Cuts at: 2395 2395
Size: 4214
NarI GG'CG_CC
Cuts at: 1645 1893 1645
Size: 248 3966
NgoMIV G'CCGG_C
Cuts at: 2393 2393
Size: 4214
NspI r_CATG'y
Cuts at: 433 1267 1767 2674 433
Size: 834 500 907 1973
Fragments arranged by size:
1973 907 834 500
PasI CC'CwG_GG
Cuts at: 175 175
Size: 4214
PciI A'CATG_T
Cuts at: 2670 2670
Size: 4214
PflMI CCAn_nnn'nTGG
Cuts at: 381 381
Size: 4214
PfoI T'CCnGG_A
Cuts at: 1020 1020
Size: 4214
PmeI GTTT'AAAC
Cuts at: 1213 1213
Size: 4214
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 622 654 3353 3385 622
Size: 32 2699 32 1451
Fragments arranged by size:
2699 1451 32 32
PstI C_TGCA'G
Cuts at: 1261 1946 1261
Size: 685 3529
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4214
PvuII CAG'CTG
Cuts at: 420 459 651 979 1639 1999 420
Size: 39 192 328 660 360 2635
Fragments arranged by size:
2635 660 360 328 192 39
RsrII CG'GwC_CG
Cuts at: 2409 2409
Size: 4214
SacI G_AGCT'C
Cuts at: 877 1228 877
Size: 351 3863
SalI G'TCGA_C
Cuts at: 1251 1251
Size: 4214
SapI GCTCTTCn'nnn_
Cuts at: 2237 2447 2237
Size: 210 4004
SbfI CC_TGCA'GG
Cuts at: 1261 1261
Size: 4214
ScaI AGT'ACT
Cuts at: 3731 3731
Size: 4214
SfcI C'TryA_G
Cuts at: 33 239 1257 1942 2935 3126 33
Size: 206 1018 685 993 191 1121
Fragments arranged by size:
1121 1018 993 685 206 191
SfoI GGC'GCC
Cuts at: 1646 1894 1646
Size: 248 3966
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4214
SmaI CCC'GGG
Cuts at: 667 1236 667
Size: 569 3645
SmlI C'TyrA_G
Cuts at: 1369 2284 2776 3038 1369
Size: 915 492 262 2545
Fragments arranged by size:
2545 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4214
SphI G_CATG'C
Cuts at: 433 1267 1767 433
Size: 834 500 2880
Fragments arranged by size:
2880 834 500
StyI C'CwwG_G
Cuts at: 265 1348 1702 265
Size: 1083 354 2777
Fragments arranged by size:
2777 1083 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1060 1797 1930 2389 2598 1060
Size: 737 133 459 209 2676
Fragments arranged by size:
2676 737 459 209 133
TatI w'GTAC_w
Cuts at: 3729 3729
Size: 4214
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 953 985 3731 3763 953
Size: 32 2746 32 1404
Fragments arranged by size:
2746 1404 32 32
Tth111I GACn'n_nGTC
Cuts at: 2011 2011
Size: 4214
XbaI T'CTAG_A
Cuts at: 1245 1245
Size: 4214
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 648 921 1709 648
Size: 273 788 3153
Fragments arranged by size:
3153 788 273
XmaI C'CCGG_G
Cuts at: 665 1234 665
Size: 569 3645
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PasI PciI PflMI PfoI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI ApaI AscI AvrII BaeI
BbsI BcgI BfrBI BmtI BsiWI BsmI BsmBI BsrGI
BssHII ClaI CspCI EcoNI EcoRV FseI FspAI HpaI
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI