Restriction map for
pF1KB6537
(Circular) (Six-base) MAPSORT of: pF1KB6537.seq Check: 6945 from: 1 to: 4214
pF1KB6537 4214 bp
With 182 enzymes: SgfI *
October 7, 2009 14:20 ..
AccI GT'mk_AC
Cuts at: 1252 1252
Size: 4214
Acc65I G'GTAC_C
Cuts at: 1230 1230
Size: 4214
AclI AA'CG_TT
Cuts at: 4054 4054
Size: 4214
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2039 2471 3218 3486 2039
Size: 432 747 268 2767
Fragments arranged by size:
2767 747 432 268
AfeI AGC'GCT
Cuts at: 214 502 1090 214
Size: 288 588 3338
Fragments arranged by size:
3338 588 288
AflIII A'CryG_T
Cuts at: 1566 2670 3647 1566
Size: 1104 977 2133
Fragments arranged by size:
2133 1104 977
AgeI A'CCGG_T
Cuts at: 2602 2602
Size: 4214
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 233 1190 233
Size: 957 3257
AlwNI CAG_nnn'CTG
Cuts at: 1519 3086 1519
Size: 1567 2647
ApaLI G'TGCA_C
Cuts at: 2984 2984
Size: 4214
ApoI r'AATT_y
Cuts at: 892 958 1218 3567 3823 892
Size: 66 260 2349 256 1283
Fragments arranged by size:
2349 1283 260 256 66
AseI AT'TA_AT
Cuts at: 20 20
Size: 4214
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4214
AvaI C'yCGr_G
Cuts at: 386 571 953 1234 386
Size: 185 382 281 3366
Fragments arranged by size:
3366 382 281 185
BamHI G'GATC_C
Cuts at: 1239 1239
Size: 4214
BanI G'GyrC_C
Cuts at: 159 1230 1644 1892 1927 159
Size: 1071 414 248 35 2446
Fragments arranged by size:
2446 1071 414 248 35
BanII G_rGCy'C
Cuts at: 393 458 797 1066 1228 2258 393
Size: 65 339 269 162 1030 2349
Fragments arranged by size:
2349 1030 339 269 162 65
BbeI G_GCGC'C
Cuts at: 163 1648 1896 163
Size: 1485 248 2481
Fragments arranged by size:
2481 1485 248
BbsI GAAGACnn'nnnn_
Cuts at: 1211 1211
Size: 4214
BciVI GTATCCnnnnn_n'
Cuts at: 2112 2873 4214 2112
Size: 761 1341 2112
Fragments arranged by size:
2112 1341 761
BclI T'GATC_A
Cuts at: 1734 1734
Size: 4214
BglII A'GATC_T
Cuts at: 1729 3766 1729
Size: 2037 2177
BlpI GC'TnA_GC
Cuts at: 1326 1326
Size: 4214
Bme1580I G_kGCm'C
Cuts at: 1839 1932 2988 1839
Size: 93 1056 3065
Fragments arranged by size:
3065 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1426 1507 1842 3986 1426
Size: 81 335 2144 1654
Fragments arranged by size:
2144 1654 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2581 2581
Size: 4214
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1146 1146
Size: 4214
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1390 2269 2761 3059 1390
Size: 879 492 298 2545
Fragments arranged by size:
2545 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3918 3918
Size: 4214
BsaAI yAC'GTr
Cuts at: 425 531 1567 425
Size: 106 1036 3072
Fragments arranged by size:
3072 1036 106
BsaBI GATnn'nnATC
Cuts at: 6 1728 3765 6
Size: 1722 2037 455
Fragments arranged by size:
2037 1722 455
BsaHI Gr'CG_yC
Cuts at: 160 1645 1893 2595 4091 160
Size: 1485 248 702 1496 283
Fragments arranged by size:
1496 1485 702 283 248
BsaWI w'CCGG_w
Cuts at: 590 990 1403 1924 2602 2876 3023 3434 590
Size: 400 413 521 678 274 147 411 1370
Fragments arranged by size:
1370 678 521 413 411 400 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 868 898 934 964 868
Size: 30 36 30 4118
Fragments arranged by size:
4118 36 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 162 958 162
Size: 796 3418
BseYI C'CCAG_C
Cuts at: 1066 1149 1639 2397 2974 3470 1066
Size: 83 490 758 577 496 1810
Fragments arranged by size:
1810 758 577 496 490 83
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 229 239 229
Size: 10 4204
BsiEI CG_ry'CG
Cuts at: 104 1756 1802 3010 104
Size: 1652 46 1208 1308
Fragments arranged by size:
1652 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 393 458 797 1002 1228 2006 2196 2988 393
Size: 65 339 205 226 778 190 792 1619
Fragments arranged by size:
1619 792 778 339 226 205 190 65
BsmI GAATG_Cn'
Cuts at: 555 555
Size: 4214
Bsp1286I G_dGCh'C
Cuts at: 393 458 797 1002 1066 1228 1839 1932 2006
Size: 65 339 205 64 162 611 93 74
Cuts at: 2006 2196 2258 2988 393
Size: 190 62 730 1619
Fragments arranged by size:
1619 730 611 339 205 190 162 93
74 65 64 62
BspEI T'CCGG_A
Cuts at: 990 3434 990
Size: 2444 1770
BspHI T'CATG_A
Cuts at: 3390 3390
Size: 4214
BspMI ACCTGCnnnn'nnnn_
Cuts at: 776 1264 1478 1780 2161 776
Size: 488 214 302 381 2829
Fragments arranged by size:
2829 488 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2506 2560 4044 4209 50
Size: 2456 54 1484 165 55
Fragments arranged by size:
2456 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2126 2126
Size: 4214
BsrFI r'CCGG_y
Cuts at: 2212 2393 2602 2212
Size: 181 209 3824
Fragments arranged by size:
3824 209 181
BssSI C'ACGA_G
Cuts at: 2485 2843 2485
Size: 358 3856
BstAPI GCAn_nnn'nTGC
Cuts at: 1591 1591
Size: 4214
BstBI TT'CG_AA
Cuts at: 2575 2575
Size: 4214
BstYI r'GATC_y
Cuts at: 1 1239 1706 1729 2064 2310 3322 3408 3420
Size: 1238 467 23 335 246 1012 86 12
Cuts at: 3420 3766 1
Size: 346 449
Fragments arranged by size:
1238 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4214
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2164 2345 2508 2164
Size: 181 163 3870
Fragments arranged by size:
3870 181 163
BtsI GCAGTG_nn'
Cuts at: 227 453 519 645 1457 227
Size: 226 66 126 812 2984
Fragments arranged by size:
2984 812 226 126 66
DraI TTT'AAA
Cuts at: 1213 1213
Size: 4214
DrdI GACnn_nn'nnGTC
Cuts at: 1920 2778 1920
Size: 858 3356
EaeI y'GGCC_r
Cuts at: 581 1799 1973 2364 2391 3736 581
Size: 1218 174 391 27 1345 1059
Fragments arranged by size:
1345 1218 1059 391 174 27
EagI C'GGCC_G
Cuts at: 1799 1799
Size: 4214
EarI CTCTTCn'nnn_
Cuts at: 139 2237 2447 139
Size: 2098 210 1906
Fragments arranged by size:
2098 1906 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 777 2422 2732 2878 4024 777
Size: 1645 310 146 1146 967
Fragments arranged by size:
1645 1146 967 310 146
EcoICRI GAG'CTC
Cuts at: 391 456 795 1226 391
Size: 65 339 431 3379
Fragments arranged by size:
3379 431 339 65
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1146 2039 2471 3218 3486 1146
Size: 893 432 747 268 1874
Fragments arranged by size:
1874 893 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 285 285
Size: 4214
EcoO109I rG'GnC_Cy
Cuts at: 1353 1353
Size: 4214
EcoRI G'AATT_C
Cuts at: 958 1218 958
Size: 260 3954
EcoRV GAT'ATC
Cuts at: 1188 1188
Size: 4214
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2233 2233
Size: 4214
FspI TGC'GCA
Cuts at: 1995 1995
Size: 4214
HaeII r_GCGC'y
Cuts at: 163 216 504 1092 1648 1896 2918 3897 163
Size: 53 288 588 556 248 1022 979 480
Fragments arranged by size:
1022 979 588 556 480 288 248 53
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 868 900 934 966 2056 2086 2088 2118 868
Size: 32 34 32 1090 30 2 30 2964
Fragments arranged by size:
2964 1090 34 32 32 30 30 2
HincII GTy'rAC
Cuts at: 267 846 1253 267
Size: 579 407 3228
Fragments arranged by size:
3228 579 407
KasI G'GCGC_C
Cuts at: 159 1644 1892 159
Size: 1485 248 2481
Fragments arranged by size:
2481 1485 248
KpnI G_GTAC'C
Cuts at: 1234 1234
Size: 4214
MluI A'CGCG_T
Cuts at: 3647 3647
Size: 4214
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 709 2885 3069 709
Size: 2176 184 1854
Fragments arranged by size:
2176 1854 184
MscI TGG'CCA
Cuts at: 1975 1975
Size: 4214
MslI CAynn'nnrTG
Cuts at: 484 2203 2330 484
Size: 1719 127 2368
Fragments arranged by size:
2368 1719 127
MspA1I CmG'CkG
Cuts at: 1325 1639 1999 3012 3257 1325
Size: 314 360 1013 245 2282
Fragments arranged by size:
2282 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2395 2395
Size: 4214
NarI GG'CG_CC
Cuts at: 160 1645 1893 160
Size: 1485 248 2481
Fragments arranged by size:
2481 1485 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4214
NdeI CA'TA_TG
Cuts at: 1010 1010
Size: 4214
NgoMIV G'CCGG_C
Cuts at: 2393 2393
Size: 4214
NruI TCG'CGA
Cuts at: 1085 1085
Size: 4214
NspI r_CATG'y
Cuts at: 678 1267 1767 2674 678
Size: 589 500 907 2218
Fragments arranged by size:
2218 907 589 500
PciI A'CATG_T
Cuts at: 2670 2670
Size: 4214
PmeI GTTT'AAAC
Cuts at: 1213 1213
Size: 4214
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3353 3385 3353
Size: 32 4182
PsiI TTA'TAA
Cuts at: 653 653
Size: 4214
PstI C_TGCA'G
Cuts at: 773 1043 1179 1261 1946 773
Size: 270 136 82 685 3041
Fragments arranged by size:
3041 685 270 136 82
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4214
PvuII CAG'CTG
Cuts at: 1639 1999 1639
Size: 360 3854
RsrII CG'GwC_CG
Cuts at: 2409 2409
Size: 4214
SacI G_AGCT'C
Cuts at: 393 458 797 1228 393
Size: 65 339 431 3379
Fragments arranged by size:
3379 431 339 65
SalI G'TCGA_C
Cuts at: 1251 1251
Size: 4214
SapI GCTCTTCn'nnn_
Cuts at: 2237 2447 2237
Size: 210 4004
SbfI CC_TGCA'GG
Cuts at: 1261 1261
Size: 4214
ScaI AGT'ACT
Cuts at: 3731 3731
Size: 4214
SfcI C'TryA_G
Cuts at: 33 769 1039 1175 1257 1942 2935 3126 33
Size: 736 270 136 82 685 993 191 1121
Fragments arranged by size:
1121 993 736 685 270 191 136 82
SfoI GGC'GCC
Cuts at: 161 1646 1894 161
Size: 1485 248 2481
Fragments arranged by size:
2481 1485 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4214
SmaI CCC'GGG
Cuts at: 1236 1236
Size: 4214
SmlI C'TyrA_G
Cuts at: 1369 2284 2776 3038 1369
Size: 915 492 262 2545
Fragments arranged by size:
2545 915 492 262
SnaBI TAC'GTA
Cuts at: 425 425
Size: 4214
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4214
SphI G_CATG'C
Cuts at: 1267 1767 1267
Size: 500 3714
SspI AAT'ATT
Cuts at: 359 359
Size: 4214
StyI C'CwwG_G
Cuts at: 107 128 1348 1702 107
Size: 21 1220 354 2619
Fragments arranged by size:
2619 1220 354 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1797 1930 2389 2598 1797
Size: 133 459 209 3413
Fragments arranged by size:
3413 459 209 133
TatI w'GTAC_w
Cuts at: 3729 3729
Size: 4214
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 103 135 560 592 3731 3763 103
Size: 32 425 32 3139 32 554
Fragments arranged by size:
3139 554 425 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 511 2011 511
Size: 1500 2714
XbaI T'CTAG_A
Cuts at: 1245 1245
Size: 4214
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1709 1709
Size: 4214
XmaI C'CCGG_G
Cuts at: 1234 1234
Size: 4214
XmnI GAAnn'nnTTC
Cuts at: 412 896 412
Size: 484 3730
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NruI NspI PciI PmeI PpiI
PsiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI SspI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI
Bpu10I BsiWI BsmBI BsrGI BssHII BstEII BstXI BstZ17I
Bsu36I ClaI CspCI DraIII FseI FspAI HindIII HpaI
MfeI NheI NotI NsiI PacI PasI PflMI PfoI
PmlI PpuMI PshAI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SrfI StuI SwaI XhoI ZraI