Restriction map for
pF1KB6543
(Circular) (Six-base) MAPSORT of: pF1KB6543.seq Check: 9153 from: 1 to: 4220
pF1KB6543 4220 bp
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AccI GT'mk_AC
Cuts at: 1258 1258
Size: 4220
Acc65I G'GTAC_C
Cuts at: 1236 1236
Size: 4220
AclI AA'CG_TT
Cuts at: 4060 4060
Size: 4220
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 452 1006 2045 2477 3224 3492 452
Size: 554 1039 432 747 268 1180
Fragments arranged by size:
1180 1039 747 554 432 268
AflIII A'CryG_T
Cuts at: 764 1572 2676 3653 764
Size: 808 1104 977 1331
Fragments arranged by size:
1331 1104 977 808
AgeI A'CCGG_T
Cuts at: 2608 2608
Size: 4220
AlwNI CAG_nnn'CTG
Cuts at: 558 1525 3092 558
Size: 967 1567 1686
Fragments arranged by size:
1686 1567 967
ApaI G_GGCC'C
Cuts at: 1131 1185 1131
Size: 54 4166
ApaLI G'TGCA_C
Cuts at: 2990 2990
Size: 4220
ApoI r'AATT_y
Cuts at: 974 1224 3573 3829 974
Size: 250 2349 256 1365
Fragments arranged by size:
2349 1365 256 250
AseI AT'TA_AT
Cuts at: 20 20
Size: 4220
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4220
AvaI C'yCGr_G
Cuts at: 1240 1240
Size: 4220
BamHI G'GATC_C
Cuts at: 1245 1245
Size: 4220
BanI G'GyrC_C
Cuts at: 1236 1650 1898 1933 1236
Size: 414 248 35 3523
Fragments arranged by size:
3523 414 248 35
BanII G_rGCy'C
Cuts at: 347 576 1131 1185 1234 2264 347
Size: 229 555 54 49 1030 2303
Fragments arranged by size:
2303 1030 555 229 54 49
BbeI G_GCGC'C
Cuts at: 1654 1902 1654
Size: 248 3972
BbsI GAAGACnn'nnnn_
Cuts at: 215 215
Size: 4220
BciVI GTATCCnnnnn_n'
Cuts at: 2118 2879 4220 2118
Size: 761 1341 2118
Fragments arranged by size:
2118 1341 761
BclI T'GATC_A
Cuts at: 1740 1740
Size: 4220
BglII A'GATC_T
Cuts at: 1735 3772 1735
Size: 2037 2183
BlpI GC'TnA_GC
Cuts at: 809 1332 809
Size: 523 3697
Bme1580I G_kGCm'C
Cuts at: 1131 1185 1845 1938 2994 1131
Size: 54 660 93 1056 2357
Fragments arranged by size:
2357 1056 660 93 54
BmrI ACTGGGnnnn_n'
Cuts at: 693 852 1188 1432 1513 1848 3992 693
Size: 159 336 244 81 335 2144 921
Fragments arranged by size:
2144 921 336 335 244 159 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2587 2587
Size: 4220
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 487 560 1056 487
Size: 73 496 3651
Fragments arranged by size:
3651 496 73
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1396 2275 2767 3065 1396
Size: 879 492 298 2551
Fragments arranged by size:
2551 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3924 3924
Size: 4220
BsaAI yAC'GTr
Cuts at: 1573 1573
Size: 4220
BsaBI GATnn'nnATC
Cuts at: 6 1734 3771 6
Size: 1728 2037 455
Fragments arranged by size:
2037 1728 455
BsaHI Gr'CG_yC
Cuts at: 193 1651 1899 2601 4097 193
Size: 1458 248 702 1496 316
Fragments arranged by size:
1496 1458 702 316 248
BsaWI w'CCGG_w
Cuts at: 674 1409 1930 2608 2882 3029 3440 674
Size: 735 521 678 274 147 411 1454
Fragments arranged by size:
1454 735 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 162 347 611 1645 2403 2980 3476 162
Size: 185 264 1034 758 577 496 906
Fragments arranged by size:
1034 906 758 577 496 264 185
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 432 688 432
Size: 256 3964
BsiEI CG_ry'CG
Cuts at: 104 1762 1808 3016 104
Size: 1658 46 1208 1308
Fragments arranged by size:
1658 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 662 810 1234 2012 2202 2994 662
Size: 148 424 778 190 792 1888
Fragments arranged by size:
1888 792 778 424 190 148
Bsp1286I G_dGCh'C
Cuts at: 347 576 662 810 1131 1185 1234 1845 1938
Size: 229 86 148 321 54 49 611 93
Cuts at: 1938 2012 2202 2264 2994 347
Size: 74 190 62 730 1573
Fragments arranged by size:
1573 730 611 321 229 190 148 93
86 74 62 54 49
BspEI T'CCGG_A
Cuts at: 3440 3440
Size: 4220
BspHI T'CATG_A
Cuts at: 3396 3396
Size: 4220
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1270 1484 1786 2167 1270
Size: 214 302 381 3323
Fragments arranged by size:
3323 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2512 2566 4050 4215 50
Size: 2462 54 1484 165 55
Fragments arranged by size:
2462 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2132 2132
Size: 4220
BsrFI r'CCGG_y
Cuts at: 2218 2399 2608 2218
Size: 181 209 3830
Fragments arranged by size:
3830 209 181
BssSI C'ACGA_G
Cuts at: 2491 2849 2491
Size: 358 3862
BstAPI GCAn_nnn'nTGC
Cuts at: 666 1597 666
Size: 931 3289
BstBI TT'CG_AA
Cuts at: 2581 2581
Size: 4220
BstXI CCAn_nnnn'nTGG
Cuts at: 842 842
Size: 4220
BstYI r'GATC_y
Cuts at: 1 470 999 1245 1712 1735 2070 2316 3328
Size: 469 529 246 467 23 335 246 1012
Cuts at: 3328 3414 3426 3772 1
Size: 86 12 346 449
Fragments arranged by size:
1012 529 469 467 449 346 335 246
246 86 23 12
BtgI C'CryG_G
Cuts at: 511 893 914 1017 511
Size: 382 21 103 3714
Fragments arranged by size:
3714 382 103 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2170 2351 2514 2170
Size: 181 163 3876
Fragments arranged by size:
3876 181 163
BtsI GCAGTG_nn'
Cuts at: 1463 1463
Size: 4220
DraI TTT'AAA
Cuts at: 1219 1219
Size: 4220
DraIII CAC_nnn'GTG
Cuts at: 666 799 666
Size: 133 4087
DrdI GACnn_nn'nnGTC
Cuts at: 1926 2784 1926
Size: 858 3362
EaeI y'GGCC_r
Cuts at: 1805 1979 2370 2397 3742 1805
Size: 174 391 27 1345 2283
Fragments arranged by size:
2283 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1805 1805
Size: 4220
EarI CTCTTCn'nnn_
Cuts at: 2243 2453 2243
Size: 210 4010
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2428 2738 2884 4030 2428
Size: 310 146 1146 2618
Fragments arranged by size:
2618 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1232 1232
Size: 4220
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 452 487 560 1006 1056 2045 2477 3224 3492
Size: 35 73 446 50 989 432 747 268
Cuts at: 3492 452
Size: 1180
Fragments arranged by size:
1180 989 747 446 432 268 73 50
35
EcoNI CCTnn'n_nnAGG
Cuts at: 280 1135 280
Size: 855 3365
EcoO109I rG'GnC_Cy
Cuts at: 507 1127 1128 1141 1148 1182 1359 507
Size: 620 1 13 7 34 177 3368
Fragments arranged by size:
3368 620 177 34 13 7 1
EcoRI G'AATT_C
Cuts at: 974 1224 974
Size: 250 3970
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 750 2239 750
Size: 1489 2731
FspI TGC'GCA
Cuts at: 2001 2001
Size: 4220
HaeII r_GCGC'y
Cuts at: 1654 1902 2924 3903 1654
Size: 248 1022 979 1971
Fragments arranged by size:
1971 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 911 943 1082 1114 2062 2092 2094 2124 911
Size: 32 139 32 948 30 2 30 3007
Fragments arranged by size:
3007 948 139 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1259 1259
Size: 4220
KasI G'GCGC_C
Cuts at: 1650 1898 1650
Size: 248 3972
KpnI G_GTAC'C
Cuts at: 1240 1240
Size: 4220
MluI A'CGCG_T
Cuts at: 3653 3653
Size: 4220
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2891 3075 2891
Size: 184 4036
MscI TGG'CCA
Cuts at: 1981 1981
Size: 4220
MslI CAynn'nnrTG
Cuts at: 538 818 2209 2336 538
Size: 280 1391 127 2422
Fragments arranged by size:
2422 1391 280 127
MspA1I CmG'CkG
Cuts at: 153 220 695 1331 1645 2005 3018 3263 153
Size: 67 475 636 314 360 1013 245 1110
Fragments arranged by size:
1110 1013 636 475 360 314 245 67
NaeI GCC'GGC
Cuts at: 2401 2401
Size: 4220
NarI GG'CG_CC
Cuts at: 1651 1899 1651
Size: 248 3972
NcoI C'CATG_G
Cuts at: 914 914
Size: 4220
NgoMIV G'CCGG_C
Cuts at: 2399 2399
Size: 4220
NspI r_CATG'y
Cuts at: 1273 1773 2680 1273
Size: 500 907 2813
Fragments arranged by size:
2813 907 500
PasI CC'CwG_GG
Cuts at: 569 569
Size: 4220
PciI A'CATG_T
Cuts at: 2676 2676
Size: 4220
PflMI CCAn_nnn'nTGG
Cuts at: 654 799 654
Size: 145 4075
PfoI T'CCnGG_A
Cuts at: 503 503
Size: 4220
PmeI GTTT'AAAC
Cuts at: 1219 1219
Size: 4220
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 322 354 3359 3391 322
Size: 32 3005 32 1151
Fragments arranged by size:
3005 1151 32 32
PpuMI rG'GwC_Cy
Cuts at: 507 507
Size: 4220
PspOMI G'GGCC_C
Cuts at: 1127 1181 1127
Size: 54 4166
PstI C_TGCA'G
Cuts at: 152 554 1073 1267 1952 152
Size: 402 519 194 685 2420
Fragments arranged by size:
2420 685 519 402 194
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4220
PvuII CAG'CTG
Cuts at: 153 220 695 1645 2005 153
Size: 67 475 950 360 2368
Fragments arranged by size:
2368 950 475 360 67
RsrII CG'GwC_CG
Cuts at: 2415 2415
Size: 4220
SacI G_AGCT'C
Cuts at: 1234 1234
Size: 4220
SalI G'TCGA_C
Cuts at: 1257 1257
Size: 4220
SapI GCTCTTCn'nnn_
Cuts at: 2243 2453 2243
Size: 210 4010
SbfI CC_TGCA'GG
Cuts at: 1267 1267
Size: 4220
ScaI AGT'ACT
Cuts at: 3737 3737
Size: 4220
SfcI C'TryA_G
Cuts at: 33 148 550 1069 1080 1263 1948 2941 3132
Size: 115 402 519 11 183 685 993 191
Cuts at: 3132 33
Size: 1121
Fragments arranged by size:
1121 993 685 519 402 191 183 115
11
SfoI GGC'GCC
Cuts at: 1652 1900 1652
Size: 248 3972
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4220
SmaI CCC'GGG
Cuts at: 1242 1242
Size: 4220
SmlI C'TyrA_G
Cuts at: 1375 2290 2782 3044 1375
Size: 915 492 262 2551
Fragments arranged by size:
2551 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4220
SphI G_CATG'C
Cuts at: 1273 1773 1273
Size: 500 3720
StyI C'CwwG_G
Cuts at: 914 1131 1354 1708 914
Size: 217 223 354 3426
Fragments arranged by size:
3426 354 223 217
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 848 1803 1936 2395 2604 848
Size: 955 133 459 209 2464
Fragments arranged by size:
2464 955 459 209 133
TatI w'GTAC_w
Cuts at: 311 3735 311
Size: 3424 796
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3737 3769 3737
Size: 32 4188
Tth111I GACn'n_nGTC
Cuts at: 2017 2017
Size: 4220
XbaI T'CTAG_A
Cuts at: 1251 1251
Size: 4220
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1715 1715
Size: 4220
XmaI C'CCGG_G
Cuts at: 1240 1240
Size: 4220
XmnI GAAnn'nnTTC
Cuts at: 871 871
Size: 4220
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BsaXI BseRI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstZ17I Bsu36I ClaI CspCI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PmlI PshAI PsiI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI ZraI