Restriction map for
pF1KB6550
(Circular) (Six-base) MAPSORT of: pF1KB6550.seq Check: 2697 from: 1 to: 4289
pF1KB6550 4289 bp
With 182 enzymes: SgfI *
April 24, 2009 13:06 ..
AccI GT'mk_AC
Cuts at: 1327 1327
Size: 4289
Acc65I G'GTAC_C
Cuts at: 1305 1305
Size: 4289
AclI AA'CG_TT
Cuts at: 4129 4129
Size: 4289
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 479 563 1280 2114 2546 3293 3561 479
Size: 84 717 834 432 747 268 1207
Fragments arranged by size:
1207 834 747 717 432 268 84
AflIII A'CryG_T
Cuts at: 480 1641 2745 3722 480
Size: 1161 1104 977 1047
Fragments arranged by size:
1161 1104 1047 977
AgeI A'CCGG_T
Cuts at: 2677 2677
Size: 4289
AlwNI CAG_nnn'CTG
Cuts at: 1594 3161 1594
Size: 1567 2722
ApaLI G'TGCA_C
Cuts at: 3059 3059
Size: 4289
ApoI r'AATT_y
Cuts at: 193 630 1293 3642 3898 193
Size: 437 663 2349 256 584
Fragments arranged by size:
2349 663 584 437 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4289
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4289
AvaI C'yCGr_G
Cuts at: 161 995 1309 161
Size: 834 314 3141
Fragments arranged by size:
3141 834 314
BamHI G'GATC_C
Cuts at: 1314 1314
Size: 4289
BanI G'GyrC_C
Cuts at: 154 1305 1719 1967 2002 154
Size: 1151 414 248 35 2441
Fragments arranged by size:
2441 1151 414 248 35
BanII G_rGCy'C
Cuts at: 1258 1303 2333 1258
Size: 45 1030 3214
Fragments arranged by size:
3214 1030 45
BbeI G_GCGC'C
Cuts at: 1723 1971 1723
Size: 248 4041
BbsI GAAGACnn'nnnn_
Cuts at: 1250 1250
Size: 4289
BbvCI CC'TCA_GC
Cuts at: 880 880
Size: 4289
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 649 683 649
Size: 34 4255
BciVI GTATCCnnnnn_n'
Cuts at: 2187 2948 4289 2187
Size: 761 1341 2187
Fragments arranged by size:
2187 1341 761
BclI T'GATC_A
Cuts at: 729 1809 729
Size: 1080 3209
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 4289
BglII A'GATC_T
Cuts at: 1804 3841 1804
Size: 2037 2252
BlpI GC'TnA_GC
Cuts at: 1401 1401
Size: 4289
Bme1580I G_kGCm'C
Cuts at: 157 1914 2007 3063 157
Size: 1757 93 1056 1383
Fragments arranged by size:
1757 1383 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1501 1582 1917 4061 1501
Size: 81 335 2144 1729
Fragments arranged by size:
2144 1729 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 998 2656 998
Size: 1658 2631
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1011 1011
Size: 4289
Bpu10I CC'TnA_GC
Cuts at: 880 880
Size: 4289
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 425 1465 2344 2836 3134 425
Size: 1040 879 492 298 1580
Fragments arranged by size:
1580 1040 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1124 3993 1124
Size: 2869 1420
BsaAI yAC'GTr
Cuts at: 964 1642 964
Size: 678 3611
BsaBI GATnn'nnATC
Cuts at: 6 398 990 1803 3840 6
Size: 392 592 813 2037 455
Fragments arranged by size:
2037 813 592 455 392
BsaHI Gr'CG_yC
Cuts at: 1720 1968 2670 4166 1720
Size: 248 702 1496 1843
Fragments arranged by size:
1843 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1478 1999 2677 2951 3098 3509 1478
Size: 521 678 274 147 411 2258
Fragments arranged by size:
2258 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 897 990 1119 1266 897
Size: 93 129 147 3920
Fragments arranged by size:
3920 147 129 93
BseYI C'CCAG_C
Cuts at: 1714 2472 3049 3545 1714
Size: 758 577 496 2458
Fragments arranged by size:
2458 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 733 733
Size: 4289
BsiEI CG_ry'CG
Cuts at: 104 1831 1877 3085 104
Size: 1727 46 1208 1308
Fragments arranged by size:
1727 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1258 1303 2081 2271 3063 1258
Size: 45 778 190 792 2484
Fragments arranged by size:
2484 792 778 190 45
Bsp1286I G_dGCh'C
Cuts at: 157 1258 1303 1914 2007 2081 2271 2333 3063
Size: 1101 45 611 93 74 190 62 730
Cuts at: 3063 157
Size: 1383
Fragments arranged by size:
1383 1101 730 611 190 93 74 62
45
BspEI T'CCGG_A
Cuts at: 3509 3509
Size: 4289
BspHI T'CATG_A
Cuts at: 243 486 705 3465 243
Size: 243 219 2760 1067
Fragments arranged by size:
2760 1067 243 219
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1339 1553 1855 2236 1339
Size: 214 302 381 3392
Fragments arranged by size:
3392 381 302 214
BsrBI CCG'CTC
Cuts at: 50 313 1025 2581 2635 4119 4284 50
Size: 263 712 1556 54 1484 165 55
Fragments arranged by size:
1556 1484 712 263 165 55 54
BsrDI GCAATG_nn'
Cuts at: 386 2201 386
Size: 1815 2474
BsrFI r'CCGG_y
Cuts at: 2287 2468 2677 2287
Size: 181 209 3899
Fragments arranged by size:
3899 209 181
BssHII G'CGCG_C
Cuts at: 129 129
Size: 4289
BssSI C'ACGA_G
Cuts at: 1184 2560 2918 1184
Size: 1376 358 2555
Fragments arranged by size:
2555 1376 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1666 1666
Size: 4289
BstBI TT'CG_AA
Cuts at: 2650 2650
Size: 4289
BstYI r'GATC_y
Cuts at: 1 270 916 1108 1314 1781 1804 2139 2385
Size: 269 646 192 206 467 23 335 246
Cuts at: 2385 3397 3483 3495 3841 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 646 467 449 346 335 269 246
206 192 86 23 12
BtgI C'CryG_G
Cuts at: 365 365
Size: 4289
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 148 919 994 2239 2420 2583 148
Size: 771 75 1245 181 163 1854
Fragments arranged by size:
1854 1245 771 181 163 75
BtsI GCAGTG_nn'
Cuts at: 1532 1532
Size: 4289
ClaI AT'CG_AT
Cuts at: 945 945
Size: 4289
DraI TTT'AAA
Cuts at: 1288 1288
Size: 4289
DraIII CAC_nnn'GTG
Cuts at: 1186 1186
Size: 4289
DrdI GACnn_nn'nnGTC
Cuts at: 1995 2853 1995
Size: 858 3431
EaeI y'GGCC_r
Cuts at: 416 1874 2048 2439 2466 3811 416
Size: 1458 174 391 27 1345 894
Fragments arranged by size:
1458 1345 894 391 174 27
EagI C'GGCC_G
Cuts at: 1874 1874
Size: 4289
EarI CTCTTCn'nnn_
Cuts at: 440 840 845 1256 2312 2522 440
Size: 400 5 411 1056 210 2207
Fragments arranged by size:
2207 1056 411 400 210 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2497 2807 2953 4099 2497
Size: 310 146 1146 2687
Fragments arranged by size:
2687 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1256 1301 1256
Size: 45 4244
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 479 563 1011 1280 2114 2546 3293 3561 479
Size: 84 448 269 834 432 747 268 1207
Fragments arranged by size:
1207 834 747 448 432 269 268 84
EcoO109I rG'GnC_Cy
Cuts at: 887 1019 1428 887
Size: 132 409 3748
Fragments arranged by size:
3748 409 132
EcoRI G'AATT_C
Cuts at: 630 1293 630
Size: 663 3626
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2308 2308
Size: 4289
FspI TGC'GCA
Cuts at: 2070 2070
Size: 4289
HaeII r_GCGC'y
Cuts at: 1723 1971 2993 3972 1723
Size: 248 1022 979 2040
Fragments arranged by size:
2040 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1129 1161 2131 2161 2163 2193 1129
Size: 32 970 30 2 30 3225
Fragments arranged by size:
3225 970 32 30 30 2
HincII GTy'rAC
Cuts at: 184 1328 184
Size: 1144 3145
HindIII A'AGCT_T
Cuts at: 778 778
Size: 4289
KasI G'GCGC_C
Cuts at: 1719 1967 1719
Size: 248 4041
KpnI G_GTAC'C
Cuts at: 1309 1309
Size: 4289
MluI A'CGCG_T
Cuts at: 3722 3722
Size: 4289
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2960 3144 2960
Size: 184 4105
MscI TGG'CCA
Cuts at: 418 2050 418
Size: 1632 2657
MslI CAynn'nnrTG
Cuts at: 710 2278 2405 710
Size: 1568 127 2594
Fragments arranged by size:
2594 1568 127
MspA1I CmG'CkG
Cuts at: 1400 1714 2074 3087 3332 1400
Size: 314 360 1013 245 2357
Fragments arranged by size:
2357 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2470 2470
Size: 4289
NarI GG'CG_CC
Cuts at: 1720 1968 1720
Size: 248 4041
NcoI C'CATG_G
Cuts at: 365 365
Size: 4289
NgoMIV G'CCGG_C
Cuts at: 2468 2468
Size: 4289
NspI r_CATG'y
Cuts at: 1342 1842 2749 1342
Size: 500 907 2882
Fragments arranged by size:
2882 907 500
PciI A'CATG_T
Cuts at: 2745 2745
Size: 4289
PflMI CCAn_nnn'nTGG
Cuts at: 150 300 150
Size: 150 4139
PmeI GTTT'AAAC
Cuts at: 1288 1288
Size: 4289
PmlI CAC'GTG
Cuts at: 964 964
Size: 4289
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3428 3460 3428
Size: 32 4257
PpuMI rG'GwC_Cy
Cuts at: 887 1019 887
Size: 132 4157
PstI C_TGCA'G
Cuts at: 380 1336 2021 380
Size: 956 685 2648
Fragments arranged by size:
2648 956 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4289
PvuII CAG'CTG
Cuts at: 1714 2074 1714
Size: 360 3929
RsrII CG'GwC_CG
Cuts at: 2484 2484
Size: 4289
SacI G_AGCT'C
Cuts at: 1258 1303 1258
Size: 45 4244
SalI G'TCGA_C
Cuts at: 1326 1326
Size: 4289
SapI GCTCTTCn'nnn_
Cuts at: 1256 2312 2522 1256
Size: 1056 210 3023
Fragments arranged by size:
3023 1056 210
SbfI CC_TGCA'GG
Cuts at: 1336 1336
Size: 4289
ScaI AGT'ACT
Cuts at: 3806 3806
Size: 4289
SfcI C'TryA_G
Cuts at: 33 376 1332 2017 3010 3201 33
Size: 343 956 685 993 191 1121
Fragments arranged by size:
1121 993 956 685 343 191
SfoI GGC'GCC
Cuts at: 1721 1969 1721
Size: 248 4041
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4289
SmaI CCC'GGG
Cuts at: 163 1311 163
Size: 1148 3141
SmlI C'TyrA_G
Cuts at: 440 1444 2359 2851 3113 440
Size: 1004 915 492 262 1616
Fragments arranged by size:
1616 1004 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4289
SphI G_CATG'C
Cuts at: 1342 1842 1342
Size: 500 3789
StyI C'CwwG_G
Cuts at: 365 521 1423 1777 365
Size: 156 902 354 2877
Fragments arranged by size:
2877 902 354 156
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1872 2005 2464 2673 1872
Size: 133 459 209 3488
Fragments arranged by size:
3488 459 209 133
TatI w'GTAC_w
Cuts at: 3804 3804
Size: 4289
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3806 3838 3806
Size: 32 4257
Tth111I GACn'n_nGTC
Cuts at: 2086 2086
Size: 4289
XbaI T'CTAG_A
Cuts at: 1075 1320 1075
Size: 245 4044
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1784 1784
Size: 4289
XmaI C'CCGG_G
Cuts at: 161 1309 161
Size: 1148 3141
XmnI GAAnn'nnTTC
Cuts at: 482 482
Size: 4289
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI ClaI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PflMI PmeI
PmlI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BfrBI BmgBI BmtI BsaXI
BsiWI BsmI BsmBI BsrGI BstEII BstXI BstZ17I Bsu36I
CspCI EcoNI EcoRV FseI FspAI HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PfoI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI ZraI