Restriction map for
pF1KB6551
(Circular) (Six-base) MAPSORT of: pF1KB6551.seq Check: 317 from: 1 to: 4226
pF1KB6551 4226 bp
With 182 enzymes: SgfI *
April 24, 2009 13:06 ..
AccI GT'mk_AC
Cuts at: 1264 1264
Size: 4226
Acc65I G'GTAC_C
Cuts at: 1242 1242
Size: 4226
AclI AA'CG_TT
Cuts at: 4066 4066
Size: 4226
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 563 2051 2483 3230 3498 563
Size: 1488 432 747 268 1291
Fragments arranged by size:
1488 1291 747 432 268
AflIII A'CryG_T
Cuts at: 1578 2682 3659 1578
Size: 1104 977 2145
Fragments arranged by size:
2145 1104 977
AgeI A'CCGG_T
Cuts at: 2614 2614
Size: 4226
AhdI GACnn_n'nnGTC
Cuts at: 187 187
Size: 4226
AleI CACnn'nnGTG
Cuts at: 737 737
Size: 4226
AlwNI CAG_nnn'CTG
Cuts at: 1531 3098 1531
Size: 1567 2659
ApaI G_GGCC'C
Cuts at: 135 1145 135
Size: 1010 3216
ApaLI G'TGCA_C
Cuts at: 2996 2996
Size: 4226
ApoI r'AATT_y
Cuts at: 630 1230 3579 3835 630
Size: 600 2349 256 1021
Fragments arranged by size:
2349 1021 600 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4226
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4226
AvaI C'yCGr_G
Cuts at: 161 923 959 992 1058 1246 161
Size: 762 36 33 66 188 3141
Fragments arranged by size:
3141 762 188 66 36 33
BamHI G'GATC_C
Cuts at: 1251 1251
Size: 4226
BanI G'GyrC_C
Cuts at: 1007 1242 1656 1904 1939 1007
Size: 235 414 248 35 3294
Fragments arranged by size:
3294 414 248 235 35
BanII G_rGCy'C
Cuts at: 135 416 1145 1168 1240 2270 135
Size: 281 729 23 72 1030 2091
Fragments arranged by size:
2091 1030 729 281 72 23
BbeI G_GCGC'C
Cuts at: 1011 1660 1908 1011
Size: 649 248 3329
Fragments arranged by size:
3329 649 248
BbvCI CC'TCA_GC
Cuts at: 614 614
Size: 4226
BciVI GTATCCnnnnn_n'
Cuts at: 2124 2885 4226 2124
Size: 761 1341 2124
Fragments arranged by size:
2124 1341 761
BclI T'GATC_A
Cuts at: 783 1746 783
Size: 963 3263
BglII A'GATC_T
Cuts at: 949 1741 3778 949
Size: 792 2037 1397
Fragments arranged by size:
2037 1397 792
BlpI GC'TnA_GC
Cuts at: 1338 1338
Size: 4226
Bme1580I G_kGCm'C
Cuts at: 135 157 1145 1851 1944 3000 135
Size: 22 988 706 93 1056 1361
Fragments arranged by size:
1361 1056 988 706 93 22
BmrI ACTGGGnnnn_n'
Cuts at: 1438 1519 1854 3998 1438
Size: 81 335 2144 1666
Fragments arranged by size:
2144 1666 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2593 2593
Size: 4226
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1071 1173 1071
Size: 102 4124
Bpu10I CC'TnA_GC
Cuts at: 614 614
Size: 4226
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 858 1180 1402 2281 2773 3071 858
Size: 322 222 879 492 298 2013
Fragments arranged by size:
2013 879 492 322 298 222
BsaI GGTCTCn'nnnn_
Cuts at: 335 706 3930 335
Size: 371 3224 631
Fragments arranged by size:
3224 631 371
BsaAI yAC'GTr
Cuts at: 1579 1579
Size: 4226
BsaBI GATnn'nnATC
Cuts at: 6 1001 1740 3777 6
Size: 995 739 2037 455
Fragments arranged by size:
2037 995 739 455
BsaHI Gr'CG_yC
Cuts at: 933 1008 1657 1905 2607 4103 933
Size: 75 649 248 702 1496 1056
Fragments arranged by size:
1496 1056 702 649 248 75
BsaWI w'CCGG_w
Cuts at: 1415 1936 2614 2888 3035 3446 1415
Size: 521 678 274 147 411 2195
Fragments arranged by size:
2195 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1069 1099 1069
Size: 30 4196
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 855 858 865 880 1123 855
Size: 3 7 15 243 3958
Fragments arranged by size:
3958 243 15 7 3
BseYI C'CCAG_C
Cuts at: 256 1651 2409 2986 3482 256
Size: 1395 758 577 496 1000
Fragments arranged by size:
1395 1000 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1768 1814 3022 104
Size: 1664 46 1208 1308
Fragments arranged by size:
1664 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 416 1168 1240 2018 2208 3000 416
Size: 752 72 778 190 792 1642
Fragments arranged by size:
1642 792 778 752 190 72
BsmBI CGTCTCn'nnnn_
Cuts at: 164 164
Size: 4226
Bsp1286I G_dGCh'C
Cuts at: 135 157 416 1145 1168 1240 1851 1944 2018
Size: 22 259 729 23 72 611 93 74
Cuts at: 2018 2208 2270 3000 135
Size: 190 62 730 1361
Fragments arranged by size:
1361 730 729 611 259 190 93 74
72 62 23 22
BspEI T'CCGG_A
Cuts at: 3446 3446
Size: 4226
BspHI T'CATG_A
Cuts at: 3402 3402
Size: 4226
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1276 1490 1792 2173 1276
Size: 214 302 381 3329
Fragments arranged by size:
3329 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1095 1124 2518 2572 4056 4221 50
Size: 1045 29 1394 54 1484 165 55
Fragments arranged by size:
1484 1394 1045 165 55 54 29
BsrDI GCAATG_nn'
Cuts at: 371 2138 371
Size: 1767 2459
BsrFI r'CCGG_y
Cuts at: 1210 2224 2405 2614 1210
Size: 1014 181 209 2822
Fragments arranged by size:
2822 1014 209 181
BssSI C'ACGA_G
Cuts at: 2497 2855 2497
Size: 358 3868
BstAPI GCAn_nnn'nTGC
Cuts at: 666 1603 666
Size: 937 3289
BstBI TT'CG_AA
Cuts at: 2587 2587
Size: 4226
BstXI CCAn_nnnn'nTGG
Cuts at: 739 739
Size: 4226
BstYI r'GATC_y
Cuts at: 1 767 949 1251 1718 1741 2076 2322 3334
Size: 766 182 302 467 23 335 246 1012
Cuts at: 3334 3420 3432 3778 1
Size: 86 12 346 449
Fragments arranged by size:
1012 766 467 449 346 335 302 246
182 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2176 2357 2520 2176
Size: 181 163 3882
Fragments arranged by size:
3882 181 163
BtsI GCAGTG_nn'
Cuts at: 1469 1469
Size: 4226
DraI TTT'AAA
Cuts at: 1225 1225
Size: 4226
DrdI GACnn_nn'nnGTC
Cuts at: 1932 2790 1932
Size: 858 3368
EaeI y'GGCC_r
Cuts at: 1811 1985 2376 2403 3748 1811
Size: 174 391 27 1345 2289
Fragments arranged by size:
2289 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1811 1811
Size: 4226
EarI CTCTTCn'nnn_
Cuts at: 814 1193 2249 2459 814
Size: 379 1056 210 2581
Fragments arranged by size:
2581 1056 379 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 102 1143 2434 2744 2890 4036 102
Size: 1041 1291 310 146 1146 292
Fragments arranged by size:
1291 1146 1041 310 292 146
EcoICRI GAG'CTC
Cuts at: 414 1166 1238 414
Size: 752 72 3402
Fragments arranged by size:
3402 752 72
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 563 1071 1173 2051 2483 3230 3498 563
Size: 508 102 878 432 747 268 1291
Fragments arranged by size:
1291 878 747 508 432 268 102
EcoO109I rG'GnC_Cy
Cuts at: 132 1141 1142 1365 132
Size: 1009 1 223 2993
Fragments arranged by size:
2993 1009 223 1
EcoRI G'AATT_C
Cuts at: 1230 1230
Size: 4226
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 768 2245 768
Size: 1477 2749
FspI TGC'GCA
Cuts at: 2007 2007
Size: 4226
HaeII r_GCGC'y
Cuts at: 1011 1660 1908 2930 3909 1011
Size: 649 248 1022 979 1328
Fragments arranged by size:
1328 1022 979 649 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 173 205 1084 1116 2068 2098 2100 2130 173
Size: 32 879 32 952 30 2 30 2269
Fragments arranged by size:
2269 952 879 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1265 1265
Size: 4226
HindIII A'AGCT_T
Cuts at: 778 778
Size: 4226
KasI G'GCGC_C
Cuts at: 1007 1656 1904 1007
Size: 649 248 3329
Fragments arranged by size:
3329 649 248
KpnI G_GTAC'C
Cuts at: 1246 1246
Size: 4226
MluI A'CGCG_T
Cuts at: 3659 3659
Size: 4226
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 916 1193 2897 3081 916
Size: 277 1704 184 2061
Fragments arranged by size:
2061 1704 277 184
MscI TGG'CCA
Cuts at: 1987 1987
Size: 4226
MslI CAynn'nnrTG
Cuts at: 710 737 2215 2342 710
Size: 27 1478 127 2594
Fragments arranged by size:
2594 1478 127 27
MspA1I CmG'CkG
Cuts at: 1337 1651 2011 3024 3269 1337
Size: 314 360 1013 245 2294
Fragments arranged by size:
2294 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1212 2407 1212
Size: 1195 3031
NarI GG'CG_CC
Cuts at: 1008 1657 1905 1008
Size: 649 248 3329
Fragments arranged by size:
3329 649 248
NgoMIV G'CCGG_C
Cuts at: 1210 2405 1210
Size: 1195 3031
NspI r_CATG'y
Cuts at: 1279 1779 2686 1279
Size: 500 907 2819
Fragments arranged by size:
2819 907 500
PciI A'CATG_T
Cuts at: 2682 2682
Size: 4226
PflMI CCAn_nnn'nTGG
Cuts at: 150 300 150
Size: 150 4076
PfoI T'CCnGG_A
Cuts at: 1024 1024
Size: 4226
PmeI GTTT'AAAC
Cuts at: 1225 1225
Size: 4226
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3365 3397 3365
Size: 32 4194
PspOMI G'GGCC_C
Cuts at: 131 1141 131
Size: 1010 3216
PstI C_TGCA'G
Cuts at: 1273 1958 1273
Size: 685 3541
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4226
PvuII CAG'CTG
Cuts at: 1651 2011 1651
Size: 360 3866
RsrII CG'GwC_CG
Cuts at: 2421 2421
Size: 4226
SacI G_AGCT'C
Cuts at: 416 1168 1240 416
Size: 752 72 3402
Fragments arranged by size:
3402 752 72
SalI G'TCGA_C
Cuts at: 1263 1263
Size: 4226
SapI GCTCTTCn'nnn_
Cuts at: 2249 2459 2249
Size: 210 4016
SbfI CC_TGCA'GG
Cuts at: 1273 1273
Size: 4226
ScaI AGT'ACT
Cuts at: 3743 3743
Size: 4226
SfcI C'TryA_G
Cuts at: 33 1269 1954 2947 3138 33
Size: 1236 685 993 191 1121
Fragments arranged by size:
1236 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1009 1658 1906 1009
Size: 649 248 3329
Fragments arranged by size:
3329 649 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4226
SmaI CCC'GGG
Cuts at: 925 1060 1248 925
Size: 135 188 3903
Fragments arranged by size:
3903 188 135
SmlI C'TyrA_G
Cuts at: 837 1159 1381 2296 2788 3050 837
Size: 322 222 915 492 262 2013
Fragments arranged by size:
2013 915 492 322 262 222
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4226
SphI G_CATG'C
Cuts at: 1279 1779 1279
Size: 500 3726
StyI C'CwwG_G
Cuts at: 696 1360 1714 696
Size: 664 354 3208
Fragments arranged by size:
3208 664 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1079 1809 1942 2401 2610 1079
Size: 730 133 459 209 2695
Fragments arranged by size:
2695 730 459 209 133
TatI w'GTAC_w
Cuts at: 483 3741 483
Size: 3258 968
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 364 396 3743 3775 364
Size: 32 3347 32 815
Fragments arranged by size:
3347 815 32 32
Tth111I GACn'n_nGTC
Cuts at: 208 1046 2023 208
Size: 838 977 2411
Fragments arranged by size:
2411 977 838
XbaI T'CTAG_A
Cuts at: 1257 1257
Size: 4226
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 426 1176 1721 426
Size: 750 545 2931
Fragments arranged by size:
2931 750 545
XmaI C'CCGG_G
Cuts at: 923 1058 1246 923
Size: 135 188 3903
Fragments arranged by size:
3903 188 135
XmnI GAAnn'nnTTC
Cuts at: 590 590
Size: 4226
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PciI PflMI PfoI PmeI PpiI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AlfI AloI AscI AvrII
BaeI BbsI BcgI BfrBI BglI BmgBI BmtI BsgI
BsiWI BsmI BsrGI BssHII BstEII BstZ17I Bsu36I BtgI
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HpaI
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PmlI PpuMI PshAI PsiI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI ZraI