Restriction map for
pF1KB6553
(Circular) (Six-base) MAPSORT of: pF1KB6553.seq Check: 4845 from: 1 to: 4226
pF1KB6553 4226 bp
With 182 enzymes: SgfI *
October 7, 2009 14:21 ..
AccI GT'mk_AC
Cuts at: 1264 1264
Size: 4226
Acc65I G'GTAC_C
Cuts at: 1242 1242
Size: 4226
AclI AA'CG_TT
Cuts at: 4066 4066
Size: 4226
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2051 2483 3230 3498 2051
Size: 432 747 268 2779
Fragments arranged by size:
2779 747 432 268
AflIII A'CryG_T
Cuts at: 330 890 1578 2682 3659 330
Size: 560 688 1104 977 897
Fragments arranged by size:
1104 977 897 688 560
AgeI A'CCGG_T
Cuts at: 2614 2614
Size: 4226
AlwNI CAG_nnn'CTG
Cuts at: 901 1531 3098 901
Size: 630 1567 2029
Fragments arranged by size:
2029 1567 630
ApaLI G'TGCA_C
Cuts at: 2996 2996
Size: 4226
ApoI r'AATT_y
Cuts at: 1230 3579 3835 1230
Size: 2349 256 1621
Fragments arranged by size:
2349 1621 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4226
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4226
AvaI C'yCGr_G
Cuts at: 200 218 1136 1246 200
Size: 18 918 110 3180
Fragments arranged by size:
3180 918 110 18
BamHI G'GATC_C
Cuts at: 1251 1251
Size: 4226
BanI G'GyrC_C
Cuts at: 321 753 816 845 960 1005 1020 1242 1656
Size: 432 63 29 115 45 15 222 414
Cuts at: 1656 1904 1939 321
Size: 248 35 2608
Fragments arranged by size:
2608 432 414 248 222 115 63 45
35 29 15
BanII G_rGCy'C
Cuts at: 570 704 888 1240 2270 570
Size: 134 184 352 1030 2526
Fragments arranged by size:
2526 1030 352 184 134
BbeI G_GCGC'C
Cuts at: 849 1024 1660 1908 849
Size: 175 636 248 3167
Fragments arranged by size:
3167 636 248 175
BbsI GAAGACnn'nnnn_
Cuts at: 327 327
Size: 4226
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4192
BciVI GTATCCnnnnn_n'
Cuts at: 2124 2885 4226 2124
Size: 761 1341 2124
Fragments arranged by size:
2124 1341 761
BclI T'GATC_A
Cuts at: 1746 1746
Size: 4226
BfrBI ATG'CAT
Cuts at: 1084 1084
Size: 4226
BglI GCCn_nnn'nGGC
Cuts at: 311 364 406 843 866 311
Size: 53 42 437 23 3671
Fragments arranged by size:
3671 437 53 42 23
BglII A'GATC_T
Cuts at: 1741 3778 1741
Size: 2037 2189
BlpI GC'TnA_GC
Cuts at: 1338 1338
Size: 4226
Bme1580I G_kGCm'C
Cuts at: 324 963 1137 1851 1944 3000 324
Size: 639 174 714 93 1056 1550
Fragments arranged by size:
1550 1056 714 639 174 93
BmgBI CAC'GTC
Cuts at: 333 333
Size: 4226
BmrI ACTGGGnnnn_n'
Cuts at: 327 1438 1519 1854 3998 327
Size: 1111 81 335 2144 555
Fragments arranged by size:
2144 1111 555 335 81
BmtI G_CTAG'C
Cuts at: 217 217
Size: 4226
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2593 2593
Size: 4226
Bpu10I CC'TnA_GC
Cuts at: 265 265
Size: 4226
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1402 2281 2773 3071 1402
Size: 879 492 298 2557
Fragments arranged by size:
2557 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3930 3930
Size: 4226
BsaAI yAC'GTr
Cuts at: 891 1579 891
Size: 688 3538
BsaBI GATnn'nnATC
Cuts at: 6 1740 3777 6
Size: 1734 2037 455
Fragments arranged by size:
2037 1734 455
BsaHI Gr'CG_yC
Cuts at: 118 846 858 1021 1657 1905 2607 4103 118
Size: 728 12 163 636 248 702 1496 241
Fragments arranged by size:
1496 728 702 636 248 241 163 12
BsaWI w'CCGG_w
Cuts at: 1415 1936 2614 2888 3035 3446 1415
Size: 521 678 274 147 411 2195
Fragments arranged by size:
2195 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 875 905 875
Size: 30 4196
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 474 1185 474
Size: 711 3515
BseYI C'CCAG_C
Cuts at: 157 166 392 626 644 683 956 1153 1651
Size: 9 226 234 18 39 273 197 498
Cuts at: 1651 2409 2986 3482 157
Size: 758 577 496 901
Fragments arranged by size:
901 758 577 498 496 273 234 226
197 39 18 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 997 1047 1164 997
Size: 50 117 4059
Fragments arranged by size:
4059 117 50
BsiEI CG_ry'CG
Cuts at: 104 417 583 1768 1814 3022 104
Size: 313 166 1185 46 1208 1308
Fragments arranged by size:
1308 1208 1185 313 166 46
BsiHKAI G_wGCw'C
Cuts at: 788 1240 2018 2208 3000 788
Size: 452 778 190 792 2014
Fragments arranged by size:
2014 792 778 452 190
Bsp1286I G_dGCh'C
Cuts at: 324 570 704 788 888 963 1137 1240 1851
Size: 246 134 84 100 75 174 103 611
Cuts at: 1851 1944 2018 2208 2270 3000 324
Size: 93 74 190 62 730 1550
Fragments arranged by size:
1550 730 611 246 190 174 134 103
100 93 84 75 74 62
BspEI T'CCGG_A
Cuts at: 3446 3446
Size: 4226
BspHI T'CATG_A
Cuts at: 3402 3402
Size: 4226
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1276 1490 1792 2173 1276
Size: 214 302 381 3329
Fragments arranged by size:
3329 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2518 2572 4056 4221 50
Size: 2468 54 1484 165 55
Fragments arranged by size:
2468 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 276 2138 276
Size: 1862 2364
BsrFI r'CCGG_y
Cuts at: 305 578 582 2224 2405 2614 305
Size: 273 4 1642 181 209 1917
Fragments arranged by size:
1917 1642 273 209 181 4
BssSI C'ACGA_G
Cuts at: 2497 2855 2497
Size: 358 3868
BstAPI GCAn_nnn'nTGC
Cuts at: 1603 1603
Size: 4226
BstBI TT'CG_AA
Cuts at: 2587 2587
Size: 4226
BstXI CCAn_nnnn'nTGG
Cuts at: 114 510 562 114
Size: 396 52 3778
Fragments arranged by size:
3778 396 52
BstYI r'GATC_y
Cuts at: 1 1076 1251 1718 1741 2076 2322 3334 3420
Size: 1075 175 467 23 335 246 1012 86
Cuts at: 3420 3432 3778 1
Size: 12 346 449
Fragments arranged by size:
1075 1012 467 449 346 335 246 175
86 23 12
BtgI C'CryG_G
Cuts at: 368 423 555 368
Size: 55 132 4039
Fragments arranged by size:
4039 132 55
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2176 2357 2520 2176
Size: 181 163 3882
Fragments arranged by size:
3882 181 163
BtsI GCAGTG_nn'
Cuts at: 1469 1469
Size: 4226
DraI TTT'AAA
Cuts at: 1225 1225
Size: 4226
DrdI GACnn_nn'nnGTC
Cuts at: 1932 2790 1932
Size: 858 3368
EaeI y'GGCC_r
Cuts at: 342 356 365 500 580 905 1046 1202 1811
Size: 14 9 135 80 325 141 156 609
Cuts at: 1811 1985 2376 2403 3748 342
Size: 174 391 27 1345 820
Fragments arranged by size:
1345 820 609 391 325 174 156 141
135 80 27 14 9
EagI C'GGCC_G
Cuts at: 580 1811 580
Size: 1231 2995
EarI CTCTTCn'nnn_
Cuts at: 637 2249 2459 637
Size: 1612 210 2404
Fragments arranged by size:
2404 1612 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 238 2434 2744 2890 4036 238
Size: 2196 310 146 1146 428
Fragments arranged by size:
2196 1146 428 310 146
EcoICRI GAG'CTC
Cuts at: 1238 1238
Size: 4226
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 2051 2483 3230 3498 2051
Size: 432 747 268 2779
Fragments arranged by size:
2779 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 1013 1204 1013
Size: 191 4035
EcoO109I rG'GnC_Cy
Cuts at: 204 996 1162 1365 204
Size: 792 166 203 3065
Fragments arranged by size:
3065 792 203 166
EcoRI G'AATT_C
Cuts at: 1230 1230
Size: 4226
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2245 2245
Size: 4226
FspI TGC'GCA
Cuts at: 2007 2007
Size: 4226
HaeII r_GCGC'y
Cuts at: 235 849 1024 1660 1908 2930 3909 235
Size: 614 175 636 248 1022 979 552
Fragments arranged by size:
1022 979 636 614 552 248 175
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1201 1233 2068 2098 2100 2130 1201
Size: 32 835 30 2 30 3297
Fragments arranged by size:
3297 835 32 30 30 2
HincII GTy'rAC
Cuts at: 1265 1265
Size: 4226
HindIII A'AGCT_T
Cuts at: 1119 1119
Size: 4226
KasI G'GCGC_C
Cuts at: 845 1020 1656 1904 845
Size: 175 636 248 3167
Fragments arranged by size:
3167 636 248 175
KpnI G_GTAC'C
Cuts at: 1246 1246
Size: 4226
MluI A'CGCG_T
Cuts at: 3659 3659
Size: 4226
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2897 3081 2897
Size: 184 4042
MscI TGG'CCA
Cuts at: 344 502 907 1048 1204 1987 344
Size: 158 405 141 156 783 2583
Fragments arranged by size:
2583 783 405 158 156 141
MslI CAynn'nnrTG
Cuts at: 112 560 2215 2342 112
Size: 448 1655 127 1996
Fragments arranged by size:
1996 1655 448 127
MspA1I CmG'CkG
Cuts at: 133 210 396 668 901 981 1061 1337 1651
Size: 77 186 272 233 80 80 276 314
Cuts at: 1651 2011 3024 3269 133
Size: 360 1013 245 1090
Fragments arranged by size:
1090 1013 360 314 276 272 245 233
186 80 80 77
NaeI GCC'GGC
Cuts at: 307 2407 307
Size: 2100 2126
NarI GG'CG_CC
Cuts at: 846 1021 1657 1905 846
Size: 175 636 248 3167
Fragments arranged by size:
3167 636 248 175
NcoI C'CATG_G
Cuts at: 555 555
Size: 4226
NgoMIV G'CCGG_C
Cuts at: 305 2405 305
Size: 2100 2126
NheI G'CTAG_C
Cuts at: 213 213
Size: 4226
NsiI A_TGCA'T
Cuts at: 1086 1086
Size: 4226
NspI r_CATG'y
Cuts at: 477 1279 1779 2686 477
Size: 802 500 907 2017
Fragments arranged by size:
2017 907 802 500
PasI CC'CwG_GG
Cuts at: 704 762 704
Size: 58 4168
PciI A'CATG_T
Cuts at: 2682 2682
Size: 4226
PflMI CCAn_nnn'nTGG
Cuts at: 1073 1073
Size: 4226
PfoI T'CCnGG_A
Cuts at: 794 794
Size: 4226
PmeI GTTT'AAAC
Cuts at: 1225 1225
Size: 4226
PmlI CAC'GTG
Cuts at: 891 891
Size: 4226
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3365 3397 3365
Size: 32 4194
PpuMI rG'GwC_Cy
Cuts at: 204 996 1162 204
Size: 792 166 3268
Fragments arranged by size:
3268 792 166
PstI C_TGCA'G
Cuts at: 464 1273 1958 464
Size: 809 685 2732
Fragments arranged by size:
2732 809 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4226
PvuII CAG'CTG
Cuts at: 396 901 981 1061 1651 2011 396
Size: 505 80 80 590 360 2611
Fragments arranged by size:
2611 590 505 360 80 80
RsrII CG'GwC_CG
Cuts at: 2421 2421
Size: 4226
SacI G_AGCT'C
Cuts at: 1240 1240
Size: 4226
SalI G'TCGA_C
Cuts at: 1263 1263
Size: 4226
SanDI GG'GwC_CC
Cuts at: 204 996 1162 204
Size: 792 166 3268
Fragments arranged by size:
3268 792 166
SapI GCTCTTCn'nnn_
Cuts at: 637 2249 2459 637
Size: 1612 210 2404
Fragments arranged by size:
2404 1612 210
SbfI CC_TGCA'GG
Cuts at: 1273 1273
Size: 4226
ScaI AGT'ACT
Cuts at: 3743 3743
Size: 4226
SfcI C'TryA_G
Cuts at: 33 460 1269 1954 2947 3138 33
Size: 427 809 685 993 191 1121
Fragments arranged by size:
1121 993 809 685 427 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 311 364 406 311
Size: 53 42 4131
Fragments arranged by size:
4131 53 42
SfoI GGC'GCC
Cuts at: 847 1022 1658 1906 847
Size: 175 636 248 3167
Fragments arranged by size:
3167 636 248 175
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4226
SmaI CCC'GGG
Cuts at: 202 220 1138 1248 202
Size: 18 918 110 3180
Fragments arranged by size:
3180 918 110 18
SmlI C'TyrA_G
Cuts at: 1381 2296 2788 3050 1381
Size: 915 492 262 2557
Fragments arranged by size:
2557 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4226
SphI G_CATG'C
Cuts at: 1279 1779 1279
Size: 500 3726
SrfI GCCC'GGGC
Cuts at: 220 220
Size: 4226
StuI AGG'CCT
Cuts at: 837 837
Size: 4226
StyI C'CwwG_G
Cuts at: 555 1205 1360 1714 555
Size: 650 155 354 3067
Fragments arranged by size:
3067 650 354 155
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 339 1167 1809 1942 2401 2610 339
Size: 828 642 133 459 209 1955
Fragments arranged by size:
1955 828 642 459 209 133
TatI w'GTAC_w
Cuts at: 3741 3741
Size: 4226
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 875 907 953 985 3743 3775 875
Size: 32 46 32 2758 32 1326
Fragments arranged by size:
2758 1326 46 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2023 2023
Size: 4226
XbaI T'CTAG_A
Cuts at: 1257 1257
Size: 4226
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 249 1197 1721 249
Size: 948 524 2754
Fragments arranged by size:
2754 948 524
XmaI C'CCGG_G
Cuts at: 200 218 1136 1246 200
Size: 18 918 110 3180
Fragments arranged by size:
3180 918 110 18
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NheI NsiI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SfcI SfiI SfoI SgfI SmaI SmlI
SpeI SphI SrfI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BpmI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI
DraIII EcoRV FseI FspAI HpaI MfeI NdeI NotI
NruI PacI PshAI PsiI PspOMI PspXI PsrI SacII
SexAI SgrAI SnaBI SspI SwaI XhoI XmnI ZraI